巻号一覧
44 巻, 6 号
選択された号の論文の21件中1～21を表示しています
- |<
- <
- 1
- >
- >|
-
川村 軍蔵, 椛山 明, 米盛 亨1978 年 44 巻 6 号 p. 567-570
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーHorizontal eyes and head movements made by a crucian carp swimming in an experimental channel set in the circulating tank were recorded cinematographically and analyzed frame-by-frame. And the width of a cone of the animal used was measured histologically and an angular extension of a cone against a lens was calculated. Though the eyes sometimes moved with the head, the approximate stable orientations of eyes, making slight fluctuations within one or two degrees, were observed in the traces of eye movements. Depending on an angular extension of a cone in temporal retinal region against a lens (0.7 degree), it was confirmed that these slight fluctuations do not interfere with vision but sharpen the edge of a retinal image, and so the compensation was substantially perfect. And it was concluded that the carp is capable of keeping retinal image stable for 200-800 ms while swimming even at relatively high speed.抄録全体を表示PDF形式でダウンロード (277K) -
産卵、孵化及び宿主上での発育今田 良造, 室賀 清邦1978 年 44 巻 6 号 p. 571-576
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe influence of water temperature on oviposition, hatching and development of P. microrchis were studied.
The results obtained are as follows:
1) The average number of eggs laid by an individual parasite per day was 1.2 at 10°C, 9.6 (4.9-19.0) at 20°C and 7.7 (3.0-9.8) at 28°C, respectively.
2) The oncomiracidium of the species hatched out mostly in 5-8 days at 20°C and 2-5 days at 28-30°C, but did not hatch out at 10°C in the experimental period of 40 days.
3) A Pseudodactylogyrus-itee eel (A. anguilla) was brought into contact with the swimming oncomiracidia in a small aquarium, and sequential observations of the young forms attaching to the host were made at 28°C. As a result, the young stages could be divided into 5.
Stage 1 (0-1 day after attachment to the host): the shape and the size of the anchor were almost the same as those of the swimming larva (oncomiracidium). Stage 2 (1-2 days): the anchor developed in size. Stage 3 (2-3 days): the bar of the anchor appeared. Stage 4 (3-4 days): the ventral and the dorsal processes of the anchor appeared. Stage 5 (4-6 days): the reflexed part of the ventral process was formed.
In 6-7 days after attaching to the host, the worm becamé adult morphologically, and actually laid eggs.抄録全体を表示PDF形式でダウンロード (428K) -
西田 睦1978 年 44 巻 6 号 p. 577-585
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe spawning habits of the dwarf Ayu-fish, Plecoglossus altivelis, in Lake Biwa were studied in the autumn of 1972-1974. Spawning grounds were mainly located in shallow riffles of lower reaches of rivers flowing into the lake. As is the case with the amphidromous type, the principal physical characters indicating an area as the spawning ground of the dwarf type seemed to be the type of substrate and water velocity. General conditions of the spawning grounds were unsettled sandy-pebble bottoms, 10-30cm in depth and 30-70cm/s in water velocity. Spawning usually occurred in the nighttime. Spawners crowded together in the spawning grounds. Prior to extruding eggs, one of the males followed and nudged a female. The female then pushed her snout to the bed of the river. Simultaneously the male dashed and swam in parallel with the female, and both fishes quivered closer together.
As to the sex ratio, males predominated in the spawners crowded in the spawning ground, while females predominated in the pool near the spawning ground in the nighttime. In contrast, in the daytime a greater proportion of males was recorded in the pool as compared with the spawning ground. Gonad conditions and stomach contents of the Ayu-fish taken from the spawning ground differed from those in the pool. These facts suggest that spawners acting in the nighttime move to the pool in the daytime. Whereas, immatures resting in the pool in the nighttime move to the riffle and feed on algae attached to stones in the daytime.抄録全体を表示PDF形式でダウンロード (819K) -
西田 清義1978 年 44 巻 6 号 p. 587-593
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーAttempts were made to prepare the myofibrils from the striated and smooth adductor muscles of giant ezo scallop. Patinopecten yessoensis. In order to eliminate the membraneous contaminants, myofibrils were washed with a buffer solution containing 1% Triton X-100.
(1) By phase-contrast microscopy, the striated structure was evident in the myofibrils from the striated adductor muscles but those from the smooth adductor muscles showed a vague feature with a relatively larger size than the former.
(2) Both myofibril preparations, which were proved homogeneous, represented essentially the same ATPase activity profiles, such as activation by Ca2+ as well as Mg2+, KCl dependency, activation by urea, and Ca-sensitivity. The measurement of inhibition of myofibrillar ATPase by azide, ouabain or quinidine concluded that the contamination of the membraneous ATPase in these myofibril preparations was negligible.
(3) Comparison of the first order rate constants for the inactivation of myofibrillar Ca2+ ATPase at 35°C showed that the stability of both myofibrils seemed to be identical.抄録全体を表示PDF形式でダウンロード (2158K) -
篠田 正俊, リン ・パン・ヨン, タン ・セン・ミン1978 年 44 巻 6 号 p. 595-600
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーSamples of trash fish were collected monthly from trawlers at Kangkar Fish Market in Singapore for 1974-1975. The amount of trash fish was 1, 574.1 ton in 1974 and 1, 155.5 ton in 1975, which constituted 35.2% and 28.8% of total Inadings, respectively.
The specimens were classified into species level or at least to the genus; 100 species representing 51 families were identified. The major components of trash fish were Mullidae, Sciaendidae, Nemipteridae, Synodontidae, Gerridae, Leiognathidae and Parapercidae, together comprising about 80% in weight of the total trash fish sampled. The composition of the trash fish can be divided into three categories, juveniles of commercially important species (31.7%) of the total trash fish in weight, low-valued species (47.6%) and species unaccepted for human consumption (21.6%).
On the size analysis of important species which form the main resources for commercial trawl fisheries, it was found that the fishes were too small to exploit from the viewpoint of the optimum size at which the population attains its maximum weight. Capture of the juveniles seems to be due mainly to the small mesh net of cod-end commonly used in the region.抄録全体を表示PDF形式でダウンロード (388K) -
劉 秀美, 橋本 周久, 瀬崎 啓次郎, 小林 弘, 中村 守純1978 年 44 巻 6 号 p. 601-606
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーIn order to contribute to the studies on ginbuna Carassius auratus langsdorfi, some attempts were made to develop a convenient method for determination of polyploidy, using 62 ginbuna specimens collected from 12 localities in Japan and one in Korea.
By karyotype analysis, it was disclosed that they were composed of 17 diploids and 45 triploids. Then a variety of tissue proteins and enzymes of those fishes were analyzed electrophoretically on starch gel and the electropherograms obtained were compared to look for any significant difference between the diploids and triploids.
Results showed that myogen and creatine kinase are useful markers for determining di- and triploidy, as far as the specimens collected from localities other than Lake Kasumigaura were concerned. The determination method which is based on the differences in their erythrocytic sizes was also satisfactory. The specimens collected from Lake Kasumigaura were all found to be triploid by karyotype analysis but some of them were erroneously determined to be diploids by the simplified techniques.抄録全体を表示PDF形式でダウンロード (392K) -
小川 和夫, 江草 周三1978 年 44 巻 6 号 p. 607-612
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーDescriptions and identifications are made of three species of Lamellodiscus parasitizing the gills of the Japanese black sea bream, Acanthopagrus schlegeli (BLEEKER) cultured at farms in Hiroshima and Shizuoka Prefs., Japan. Among the three species, one is identified as L. spari ZHUKOV, 1970 and the other two, L. takitai n.sp. and L. japonicus n.sp. L. takitai n.sp. somewhat resembles L. verberis EUZET et OLIVER, 1967 in the shape of the copulatory organ, but may be distinguished from the latter species in the shape of the vagina. L. japonicus n.sp. may differ from all other Lamellodiscus species in the shape of the copulatory organ.
Morphologically, the following characteristics should be noted. 1) The vas deferens turns round the left intestine. This may possibly be common to all Lamellodiscus species, although it has been described as not turning round the intestine in all previous papers. 2) The prostatic reservoir is longitudinally bipartite. 3) In L. spari, the vagina opens on the right side of the body, which seems to be an exceptional case in the genus.抄録全体を表示PDF形式でダウンロード (476K) -
小川 和夫, 江草 周三1978 年 44 巻 6 号 p. 613-618
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーDescriptions and identifications are made of seven species of Gyrodactylus collected from four species of cultured freshwater fish. Three species of Gyrodactylus were obtained from the ayu (Plecoglossus altivelis); G. japonucus KIKUCHI, 1929, G. tominagai n.sp. and G. plecoglossi n.sp. G. tominagai n.sp. is closely related to G. japonicus, but may be distinguished mainly by the shape and size of the marginal book. G. plecoglossi n.sp. resembles G. japonicus and G. tominagai, but differs in the smaller anchor and different shape and size of the marginal hook. G. kherulensis ERGENS, 1974 and G. sprostonae LING, 1962 were collected from the carp (Cyprinus carpio); they are the first recorded cases in Japan. G. nipponensis n.sp. is described from the Japanese eel (Anguilla japonica). This differs in the larger anchor and the smaller sickle of the marginal hook from the most closely related species, G. anguillae ERGENS, 1960, which is redescribed for comparative study based on the specimens from the European eel (Anguilla anguilla) in England.抄録全体を表示PDF形式でダウンロード (387K) -
谷口 順彦, 田島 健司1978 年 44 巻 6 号 p. 619-622
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーA distinct variation was found in the electropherogram of esterase of the red sea bream Pagrus major (TEMMINCK et SCHLEGEL) collected from the waters of Japan and the offsprings bred artificially by natural spawnieg method in the culture pond. It was presumed that there are eight alleles at the esterase locus on the basis of the electrophoretic polymorphism. Seventeen of 36 phenotypes theoretically expected were found. The validity of genetic model presumed on the esterase locus was ascertained by the test of goodness-of-fit of the phenotype frequencies and the Hardy-Weinberg expectations. The frequencies of allele d ranged from 0.116 to 0.138, allele e from 0.531 to 0.643, allele f from 0.185 to 0.281, in natural samples. In the frequencies of alleles d, e and f, there was no statistical difference among the natural samples. On the other hand, variation in the allele frequencies of esterase locus caused by the “WRIGHT effect” (random genetic drift) was actually observed among the samples obtained by artificial breeding.抄録全体を表示PDF形式でダウンロード (1098K) -
橋本 康平, 佐々木 幸夫, 小原 佑一, 松沢 淑雄1978 年 44 巻 6 号 p. 623-629
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe absorption, tissue distribution, and excretion of 2-mercaptobenzothiazole (M) were investigated using 14C-M.
When a carp was starved after administration of 14C-M, after 1-h the radioactivity which remained in the intestine was 45% of the dose, and after 72-h the activity in the bile was about 9% and in the other tissues was less than 0.05% of the dose.
This radioactive chemical in the bile was determined to be benzothiazole-2-mercaptoglucuronide.
On the other hand, when a carp was fed after administration of 14C-M, after 72-h the radioactivity in the bile was less than 0.3% and in the other tissues was negligible in amount. Thus, about 100% of the dose was excreted into the water.抄録全体を表示PDF形式でダウンロード (1558K) -
山口 勝巳, 緒方 武比古1978 年 44 巻 6 号 p. 631-637
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーA dark blue chromoprotein was isolated and purified from the ovary of a turban shell, Turbo cornutus, by ammonium sulfate fractionation and Sepharose-4B gel fltration. The chromoprotein behaved homogeneously both in starch gel electrophoresis and in ultracentrifugation. Its absorption maxima appeared at 278, 374, 535-540, and 600-605 nm in acidic solution. The molecular weight was 540, 000 with s20, w of 16S. The protein moiety which had been separated from the chromophore by the HCl-acetone treatment was analyzed for amino acid composition. The chromophore was proved to be a new bilatriene on the basis of its U. V. and visible spectra, its thin layer chromatographic behavior, a coloration test, and a chromic acid-dichromate degradation test.抄録全体を表示PDF形式でダウンロード (1002K) -
吉中 礼二, 佐藤 守, 池田 静徳1978 年 44 巻 6 号 p. 639-641
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe collagenolytic activity was assayed in the contents of the alimentary canal of rainbow trout, weighing about 200g, which had been given either eviscerated carp fingerlings or a commercial diet for rainbow trout. In both cases, practically no activity was detected in the contents of the stomach, whereas high activity was detected in the contents of the pyloric caeca and the intestine. When considered with the finding in a previous study that collagenase in the digestive system of fishes is possibly of pancreatic origin, this finding indicates that collagenase is secreted into the intestine at a point close to the pylorus.
In order to ascertain further the participation of collagenase in the digestion of collagen by rainbow trout, the digestibility of the collagen in the freeze-dried carp meal was examined by an indirect method using chromic oxide. It was found that its apparent digestibility was about 45%, and that the ingested collagen was digested and absorbed in the course of passage through the pyloric caeca and the pre- and mid-intestine.
These findings indicate that collagenase in the alimentary canal of rainbow trout participates in the digestion of the ingested collagen.抄録全体を表示PDF形式でダウンロード (281K) -
Enterobacter cloacae UFF-107による褐変前駆物質の生成機構藤田 八束, 松本 寿子, 松田 敏生1978 年 44 巻 6 号 p. 643-651
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe brown discoloration by microorganisms in fish jelly products is very interesting as a reaction involved in the microbial deterioration of foods. In this paper, the mechanism of brown discoloration caused by the microorganism Enterobacter cloacae UFF-107 was studied, and the following results were obtained.
1. The browning precursor which was participating in the reaction with various amino acids or proteins to form the brown substance was confirmed to be formed from glucose or 2-ketogluconic acid by intact cells of E. cloacae UFF-107.
2. We tried to isolate the browning precursor from the fermented liquid containing glucose. This was not successful because the browning precursor was very unstable and decomposed very easily. So, we attempted to isolate it according to KATZNELSON et al., AIDA et al., or KONDO's method. By use of this method, Ca-2, 5-diketogluconate was isolated from the fermented liquid containing Ca-2-ketogluconate. The obtained substance was a pale yellow Casalt. The aqueous solution containing the Ca-salt and glycine formed a brown substance when it was heated for 1 minute at 100°C or incubated for 1 hour at 37°C.
3. We tried to detect the Ca-salt by descending paper chromatography using a solvent system of water saturated isobutylic acid. The RG value was 0.52. KATZNELSON et al. or HENDERSON et al. reported that the RG value for this compound was 0.54 or from 0.57 to 0.61. With p-anisidine hydrochloride as a developing agent, the Ca-salt appeared as a pale yellow spot. This Ca-salt reduces FEHLING's solution and ammoniacal silver nitrate in the cold. PINOFF's reaction, SELIWANOFF's reaction, and sodium nitroprusside reaction were all positive. The ferric chloride reaction and orcinol test was negative.
These results agreed well with those reported by KATZNELSON et al. and AIDA et al. The preparation obtained from the fermented liquid of Gluconobacter melanogenus IAM-1836 showed a behavior similar to that of Ca-2, 5-diketogluconate. From these data, The browning precursor obtained was identified as 2, 5-diketogluconic acid.
4. Gluconobacter suboxydans IAM-1829, G. gluconicus IAM-1815, G. melanogenus IAM-1836, and G. melanogenus IAM-1822 were inoculated individually into broth media containing glucose, and were incubated for 2 days at 30°C. After incubation, various amino acids or proteins were then added to the culture media. Only the culture media fermented by G. melanogenus IAM-1836 or G. melanogenus IAM-1822 formed a brown substance when heated at 100°C for 7 minutes.抄録全体を表示PDF形式でダウンロード (777K) -
サンマによるハマチの餌料性疾患とB1の投与効果石原 忠, 原 研治, 中山 英則, 保田 正人1978 年 44 巻 6 号 p. 653-657
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe existence of thiaminase I in anchovy, Engraulis japonica, and saury, Coiolabis saira, was elucidated in a previous paper. And it is well known that yellowtail which are fed only anchovy for a long period develop a feedinduced disease.
The present study investigated whether the yellowtail fed with saury showed the same disease as the yellowtail fed with anchovy or not. Yellowtail with an average weight of 13.3g were fed saury with or without added thiamine nitrate for 47 days. The thiamine nitrate used was coated with fat in order to prevent its decomposition by thiaminase.
The yellowtail fed saury showed a feedinduced disease, with symptoms such as anorexia, dark colored body, retarded growth, loss of equilibrium, and high mortality. In these fish, the contents of thiamine in ordinary muscle, the triglyceride and cholesterol in serum, the hematocrit value and hemoglobin in the blood all significantly decreased, while the pyruvic acid in the blood increased remarkably. These symptoms of the yellowtail were in agreement with those of the yellowtail fed with anchovy. On the other hand, the yellowtail fed during the entire period with the saury to which was added the thiamine nitrate showed good growth and no mortality.抄録全体を表示PDF形式でダウンロード (336K) -
カタクチイワシによつて飼育したハマチのチアミン要求量石原 忠, 原 研治, 八木 基明, 保田 正人1978 年 44 巻 6 号 p. 659-664
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe thiamine requirement of the yellowtail fed with anchovy was investigated. Yellowtail with an average weight of 13.3g were fed anchovy with or without added thiamine nitrate for 48days. The tiamine nitrate used was coated with fat in order to prevent its decomposition by the thiaminase I present in the anchovy.
Both the fish that were fed anchovy as the sole food source during the entire period and those that received an additional 0.25mg of thiamine nitrate per 1kg of body weight per day, showed retardation of growth and high mortality. At the end of the experiment, the survival rate of the above fish were 4.4 and 30%, respectively. In these fish, the thiamine in ordinary muscle, serum triglyceride, serum cholesterol, hemoglobin, the hematocrit value, and the number of erythrocytes all decreased remarkably, and the pyruvic acid in the blood increased. However, these signs of thiamine defferency could be completely prevented by administering more than 1mg per day of thiamine nitrate per 1kg of body weight of the yellowtail.抄録全体を表示PDF形式でダウンロード (441K) -
森下 日出旗, 佐野 利彦, 神谷 典清, 奥田 正男1978 年 44 巻 6 号 p. 665-671
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe effect of hot water extract (CE, I) of chlorella cells on the growth and protein synthesis of Vibrio alginolyticus was studied and compared with its effect on of Vibrio parahaemolyticus, a slightly halophilic bacterium, and Escherichia coli, a non-halophilic bacterium. A remarkable growth-promoting effect of CE was found for V. alginolyticus but not for E. coli. The CE was fractionated by Sephadex G-25 column chromatography into two fractions (II and III). Fraction II was composed of high molecular weight (above 5, 000) compounds, and fraction III of low molecular weight (below 5, 000) compounds. Fraction II contained amino acids in higher concentration than fraction III, but it contained a smaller amount of S-containing amino acids. Fraction III exhibited a marked stimulatory effect on the growth of V. alginolyticus; fraction II did not show such a remarkable effect. This effect of fraction III might be due to easily utilizable amino acids, especially the S-containing amino acids contained in the fraction. Fraction II was further fractionated into four fractions (IV, V, VI, and VII) by DEAE-cellulose column chromatography. Fractions IV, V, and VI contained neutral sugar and protein in relatively high concentrations, but nucleic acids in very low concentration. Fraction VII showed the highest concentration of nucleic acids among the four fractions. Fractions V and VII had a growth-stimulating effect, but fractions IV and VI did not. Fraction VII showed the most remarkable effect on the growth and protein synthesis of V. alginolyticus.
Based on these results, it is suggested that (1) high molecular weight fractions of CE, which contain thymine, adenine, guanine, cytosine and uracil as their main components, play an important role in the stimulation of growth and protein synthesis of V. alginolyticus, and that (2) amino acids in the low molecular weight fraction, especially S-containing amino acids, also stimulate the growth.抄録全体を表示PDF形式でダウンロード (478K) -
逆井 直利, 林 和也, 中島 義一, 斉藤 伸生, 柴 真1978 年 44 巻 6 号 p. 673-675
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーA convenient assay method for protease of the Antarctic Krill, Euphausia superba, which is well suited for measurements on a ship is proposed.
A paper disk (diameter: 8mm) containing extracted juice of a Krill sample was put on the surface of a 10ml-casein-agar plate (1.5% agar 0.4% casein, 0.1M phosphate buffer, pH 7.0) and kept at 30°C for a definite time. The diameter (mm) of the clear halo which appeared after the treatment of the plate with 5% trichloroacetic acid was measured.
In order to calculate the proteolytic activity (PU) of Krill samples, the solution of pancreatin of known activity was used as a reference standard. The relationship between the logarithm of the enzyme concentration and the diameter of the halo was definitely linear.抄録全体を表示PDF形式でダウンロード (404K) -
竹内 俊郎, 渡辺 武, 荻野 珍吉1978 年 44 巻 6 号 p. 677-681
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーFeeding experiments were conducted twice, Experiments I and II, in order to determine the effect of increasing the levels of dietary lipids in a high protein diet on the growth of rainbow trout. In both experiments, fish were fed on 54% casein diets supplemented with lipids ranging from 5-20%, which contained enough ω3 fatty acids to satisfy the EFA requirement of rainbow trout. In Experiment I, there was no significant difference in the growth rate among the experimental groups, although a slightly better growth was obtained in fish receiving 15% lipid. In Experiment II, both the growth rate and feed conversion were excellent in fish fed a diet containing 20% lipid, low in fish fed a diet containing 5% lipid, and there was little difference between the weight gain of fish fed on diets containing 10% or 15% lipid.
Fish receiving 20% lipid, however, showed a great increase in the amount of fat in the peritoneal cavity and viscera. This suggests that the excessive energy of the diet was deposited as body fat, causing an increase in fish weight. The fact that although the growth rate, feed conversion, and protein efficiency ratio were slightly lower in fish fed a diet containing 5% lipid, there were, in general, no significant differences among experimental fish receiving diets containing more than 10% lipids, suggests that addition of approximately 10% lipid to the high protein diets is sufficient to maintain normal fish growth.抄録全体を表示PDF形式でダウンロード (334K) -
竹内 俊郎, 渡辺 武, 荻野 珍吉1978 年 44 巻 6 号 p. 683-688
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーA feeding trial was conducted in order to determine the optimum ratio of protein to lipid in diets of rainbow trout by feeding them various diets containing different amounts of lipids (5-20%) in different protein levels (16-48%). The weight gain and feed conversion improved in each dietary protein level as the dietary lipid levels were increased, reaching a maximum in the 35% protein diets with 15-20% lipid. The higher the lipid content or the lower the protein content in the diets, the higher the rate of protein which was retained, suggesting that protein was efficiently utilized by increased levels of dietary lipids. The lower the lipid content or the higher the protein content in the diets, the higher the retention rate of lipid which was observed, suggesting that some part of the dietary protein was converted to body fat. The lower the protein content, the higher the values which were obtained for PER and NPU. These values of casein determined at different protein and lipid levels indicate that the addition of more than 10% dietary lipids is necessary in order to increase the efficiency of protein utilization.
It has thus been shown that the optimum ratio of protein to lipid in diets of rainbow trout is 35% to 15-20%. and that at these levels of lipids dietary protein can be reduced from 48% to 35% with no loss in weight gain.抄録全体を表示PDF形式でダウンロード (385K) -
原 彰彦1978 年 44 巻 6 号 p. 689-693
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーThe sexual differences in serum proteins of mature chum salmon, Oncorhynchus keta, were studied by polyacrylamide disc electrophoresis and thin-layer gel chromatography on Sephadex G-200. A protein band was observed specifically in the serum of female fish by disc electrophoresis. Thin-layer gel chromatography combined with autoradiography with 59Fe disclosed that this female-specific serum protein migrated as a protein of large molecular size (around 600, 000) and that the 59Fe added was specifically bound to this protein, which was clearly different from transferrin.
The protein was purified with hydroxylapatite column chromatography followed by gel filtration on Sepharose 6B. The sedimentation and diffusion coefficients were 12.5S and 1.87×10-7 cm2/s respectively, from which the molecular weight was calculated as 630, 000, in good agreement with the value obtained by gel filtration. Some chemical properties of the female-specific serum protein seemed to be similar to those of amphibian vitellogenin, which is a precursor of egg yolk proteins.抄録全体を表示PDF形式でダウンロード (947K) -
関口 秀夫1978 年 44 巻 6 号 p. 695
発行日: 1978/06/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (130K)
- |<
- <
- 1
- >
- >|