日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
48 巻, 10 号
選択された号の論文の25件中1~25を表示しています
  • 宗清 正廣, 篠田 正俊, 杉村 治
    1982 年 48 巻 10 号 p. 1371-1374
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    A method was described for reading rings appearing on vertebral centra. This method was to make Polyvinyl alcohol replicas from vertebra centra for convenience of reading rings on their concave sufaces. As far as the materials examined by the authors is concerned, polyvinyl alcohol was found to be best. For making replica, polyvinyl alcohol solution is first applied on the vertebral centrum, after removing the remnant tissue and fat. Then the film is peeled off after drying, and mounted betweem two glass slides for reading rings with binocular microscope.
    As a preliminary work, the method was used to make replicas from vertebral centra of two species, yellowtail Seriola quinqueradiata and ribbon fish Trichiurus lepturus. These replicas seemed to be better than opercular bone and otolith which have been generally used for age estimation of these species. Full advantage could be taken of the replica of vertebral centrum on ageing fish, especially those whose otolith, opercular bone and scale did not show any age marks.
  • 桑原 昭彦, 鈴木 重喜
    1982 年 48 巻 10 号 p. 1375-1381
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Examinations were made of the vertical distribution and gut contents of larvae of flounder Paralichthys olivaceus (TEMMINCK et SCHLEGEL) collected with larvae nets (160cm mouth diameter and GG54 mesh size) in the western Wakasa Bay belonging to the Japan Sea during a period from May to July 1981.
    The larvae distributed abundantly in the shallow waters, especially in the waters between 25m and 50m depth. From estimation of the vertical distribution of the larvae, less than 3.0mm BL with a yolk-sac, it was implied that the flounder eggs hatched out around a depth of 25m.
    The larvae fed mainly on Oikopleura longicauda, Evadne nordmanni, Penilia avirostris, Paracalanus parvus and copepod nauplii. There appeared a variety of diets found in the gut through the survey period. The monthly varation may closely related with a species composition of plankton community in the area. According to the relationship between the gut contents and plankton compositions in the area, the larvae has selection for plankton species. The size of prey increased with the growth of larvae in body length, and the larvae showed a favorable size selection of prey.
  • 池田 弥生, 見奈美 輝彦
    1982 年 48 巻 10 号 p. 1383-1388
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The changes in various blood constituents were examined in both yellowtail Seriola quinqueradiata infected naturally and inoculated with Streptococcus sp. The fish weighing about 150g were injected intraperitoneally with 107 cells of Streptococcus per fish. Examination was carried out in September. The water temperature was about 26°C.
    The size of spleen, kidney and heart (% of body weight) in naturally infected fish increaded, but that of hepatopancreas did not. Increase in neutrophil count was recognized in both naturally infected and inoculated fish that the symptoms were noticeable, but increase in lymphocyte count was recognized in only the former. Streptococcus sp. infection was associated with increcially proportional to the degree of external symptoms. Erythrocyte count, hrmoglobin level and serum levels of activities of total protein, albumin, globulin, GOT and GPT, remained unchanged. The low nutritional state in naturally infected fish was not especially recognized, but decrease in serum urea-N level suggests that protein metabolism in the fish is low.
  • 北原 武
    1982 年 48 巻 10 号 p. 1389-1395
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    This paper attempts to derive a reasonable method for simply estimating the size of an exploited population which migrates in a certain direction, under the assumption that emigrant number in an area is equal to the immigrant number in its adjacent areas. In deriving the method, it was assumed that the emigration rate was the same in the considered areas and that the natural mortality rate was negligibly small as compared with the emigration rate. An application of the derived method gave about 0.1 during 5 days an an estimate of the emigration rate of the swimming crab Portunus trituberculatus population along the Buzen District, Fukuoka Pref., in 1978 and 1979. The present method can not be used for the populations that immigrate continuously.
  • 川村 軍蔵, 清原 貞夫, 米盛 亨, 柿本 亮, 折田 昭一
    1982 年 48 巻 10 号 p. 1397-1400
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    This study was conducted to examine a turnover of fish school attracted to a fish lamp during fishing operation. Fishes drawn to a fish lamp were marked by being fed dyed chum bait (minced anchovy with food coloring) and then hooked, having stomach contents examined on board. The catch was recorded at certain intervals of time and the school was monitored by a fish finder. The food coloring appeared to have an adverse effect on spooted macherel. Those hooked before and after the use of dyed chum bait showed clear differences in stomach contents or body size. It was evident that a turnover of shcools under the fish lamp took place. The chemical effect of the food coloring was tested electrophysiologically on the minnow.
  • 吉田 陽一, 沼田 克己
    1982 年 48 巻 10 号 p. 1401-1405
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Lgrge scale red tides of Chattonella sp., which is smaller than Chattonella antiqua (HADA) ONO, took plave at the northern part of Kagoshima Bay in 1977 and at Suho Nada, which is located in the western part of the Seto Inland Sea, in 1979.
    In order to clarify the mechanisms of the accumulation and movement of Chattonella sp. and of the occurrence and disappearance of these red tides, the relations between the accumulation and factors such as velocity and direction of tide and wind were examined.
    The results suggested that the tidal current and wind acted togerher during the accumulation of Chattonella sp. in the Kagoshima Bay, whereas the tidal current mainly acted alone during the accumulation in the Suho Nada. The extraordinary high number (138, 000cells/ml) of the plankton which were found at the innermost part of Kagoshima Bay would be caused by the tidal current and wind acting together with very strong force.
  • 塩見 一雄, 稲岡 計, 山中 英明, 菊池 武昭
    1982 年 48 巻 10 号 p. 1407-1410
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The toxins of xanthid crab Atergatis floridus from Chiba were purified successively on activated charcoal, Bio-Gel P-2, and Bio-Rex 70, and then analyzed by thin layer chromatography and electrophoresis. No significant different difference was observed between August and November specimens. In Bio-Rex 70 column chromatography, 99% of the toxins were eluted with 0-0.03 and 1% with1.5N acetiacid; these results indicated that the crabs in Chiba contain gonyautoxins as major toxic components, differing from those in okinawa in which saxitoxin and neosaxitoxin are predominant. three toxins, none of which were in accord with the standards used, were detected in the gonyautoxin fraction. One of them was the major toxic component, since its relative ratio to the total toxicity was as high as 85-91%.
  • 佐藤 実, 佐藤 美和
    1982 年 48 巻 10 号 p. 1411-1414
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The following method was established for quantitative determination of two analogous compounds, meso-α-iminodipropionic acid (IDPA) and D-α-iminopropioacetic acid (IPAA), found in marine molluscs by the authors. The procedure is to combine electrophoretic separation with densitometric determination. This gave a high recovery, over 96 per cent for both IDPA and IPAA. The distribution of the two compounds was studied in a variety of aquatic organisms by using this method. It was found that they had a very limited distribution of the species belong-ing only to the two phyla of animal kingdom, Mollusca and Annelida, which were all organismspertaining to the sea.in mollusca, IDPA and IPAA were detected in many species of three main classes: Gastropoda, Pelecypoda, and Cephalopoda. Although there is no obvious relationship between their distributions and class, it can be seen that both compounds are distributed more widely in Pelecypoda than in other classes. The distribution pattern of the two compounds strongly resembled that of octopine.
  • 佐藤 実, 佐藤 美和, 土屋 靖彦
    1982 年 48 巻 10 号 p. 1415-1419
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The biosynthesizing ability of both meso-α-iminodipropionic acid (IDPA) and D-α-imino-propioacetic acid (IPAA) in scallop Patinopecten yessoensis was investigated by incubating four 14C-labeled compounds: L-alanine, glycine, propionate and pyruvate, with the adductor muscle and mid-gut gland homogenates. Incorporation of radioactivity into both IDPA and IPAA from three of these radioactive compounds (not from propionate) was confirmed in the adductor muscle, but not in the mid-gut gland. The incorporation rates and ratios of each radioactive compound in IDPA and IPAA suggest that their common precursor was pyruvate and that L-alanine or glycine participated as the partner. And also their metabolisms seem quite active judged from the incor-poration rates of 14C-labeled L-alanine and glycine, which amounts added to the homogenates were extremely small as compared with their native contents.
    The biological role of IDPA and IPAA in molluscs, is like that of octopine, contributing to the regulation of the redox balance of invertebrate muscle tissue on behalf of the lactate in vertebrate. For they were biosynthesized only in the muscle tissue under the presence of NADH from amino acids and pyruvate, which is an end product of glycolysis.
  • 神谷 久男, 緒方 京子
    1982 年 48 巻 10 号 p. 1421-1425
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Three different molecular species of agglutinins were found to be persent in the coelomic fluid of Balanus (Megabalanus) roseus. By affinity chromatography on acid-treated Sepharose 4B and subsequent gel filtration on Sephadex G-100, tow of them were obtained in purified forms. A major active component, BRA-3, reacted with rabbit erythrocytes at a concentration as low as 0.36 μg/ml. A minor component, BRA-2 weakly reacted with rabbit blood cells but strongly with human O cells. the molecular weights of BRA-2 and BRA-3 were found to be 120000 and 43000 daltons, respectively. The purified agglutinins were inhibited in varying degrees by galactose lactose, glucuronic acid, galacturonic acid, and N-acetyIneuraminic acid.
  • 山本 啓之, 絵面 良男, 木村 喬久
    1982 年 48 巻 10 号 p. 1427-1431
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Antibacterial action in seawater was examined. When bacterial cells were inoculated intonatural seawater or 8 μm filtrated seawater, not only Escherichia coli but also the five species of halophilic or marine bacteria inoculated showed a rapid decline in number or turbidity. On the other hand, the bacteria inoculated did not decrease in autoclaved or 0.22 μm filtrated seawater. This phenomenon was observed in all tested seawater. These results suggest that natural seawater contains some biological agents which control the bacterial population.
  • 山本 啓之, 絵面 良男, 木村 喬久
    1982 年 48 巻 10 号 p. 1433-1439
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Changes of microbial population were studied in seawater and in seawater containing sand; both were inoculated with Vibrio parahaemolyticus. The viable cell number of V. paranaemolyticus decreased in all samples of the natural seawater and of the seawater containing natural sea sand. Flagellates and ciliates were observed in these samples . And amoebae, bdellovibrio and myxobacteria, plaque-forming microorganisms, increased in number in these samples following a decline of V. parahaemolyticus. But the effectiveness of predation and parasitism decreased in the samples containing sand. The results obtained in this study indicate that the decline of V. parahaemolyticus can be attributed to indigenous predators and parasites in the sea.
  • 金沢 昭夫, 手島 新一, 今田 中伸哉, 今田 克, 井上 顕信
    1982 年 48 巻 10 号 p. 1441-1444
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The present paper deals with the uptake of radioactivity into the organs and tissues in the red sea bream Chrysophrys major, 24h after injection of [14C] eicosapentaenoic acid (20:5ω3). [14C] 20:5ω3 was biologically prepared by incubating a marine Chlorella with[1-14C] linolenic acid. The incorporation of radioactivity into the organs and tissues was determined by radioactive measurements with a liquid scintillation counter after combustion of each tissue or organ and also by autoradiography of the total body of the red sea bream.
    The results of the present study showed that radioactivity from [14C] 20:5ω3 was mainly incorporated into the gall bladder, swim bladder, liver, and pyloric caecum, suggesting that dietary 20:5ω3 may take part in some physiologically important metabolism in these organs.
  • 伊東 信, 森永 哲夫, 北御門 学
    1982 年 48 巻 10 号 p. 1445-1452
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    A new method for the detection of keratan sulfate in connective tissue has been developed without its prior isolation from a mixture of glycosaminoglycan. The method relies on the specificaction of an endo-β-galactosidase from Flavobacterium sp. on keratan sulfate.
    Besides being found in mammals, keratan sulfate is widely distributed in the connective tissues of teleost; it is especially abundant in the skin, cornea, and caudal fin of Pacific mackerel, and in the cornea of horse mackerel.
    From the skin of Pacific mackerel, a crude preparation of polysaccharide was obtained, which contained a large amount of carbohydrate chains susceptible to endo-β-galactosidase. The fraction with the carbohydrate chains was isolated by treating the preparation with hyaluronidase and chondroitinase ABC. The purified substance was characterized using chemical and infrared spectrum analyses, and was indentified as keratan sulfate. This paper is the first report to demonstrate the presence of keratan sulfate in skin tissue.
  • 山田 真知子, 武居 薫, 鶴田 新生
    1982 年 48 巻 10 号 p. 1453-1456
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Dokai Bay (Kitakyushu City, Japan) is classified as an extremely enutrophic area; it receives various industrial waste waters. Four kinds of industrial effluents, three from the processes of producing dyestuff, coke, vegetable oil and fat, and the fourth from secondary sewage treatment facilities, are the main sources of the organic pollution of this bay.
    In the cases of five species and strains of marine phytoplanktonm cultured by the medium containing three of these effluents not sewage effluent at the concentrations of 0.01, 0.1, 1.0, and 10 percent, the growth of Skeletonema costatum and Olisthodiscus sp., which cause red tides in Dokai Bay, were stimulated at the early logarithmic phase by 1.2 to 2.6 times compared with those of their controls. And maximum cell numbers of two strains of S. costatum and two strains of Olisthodiscus sp. which were isolated from Dokai Bay and the other seas increased by 1.2 to 14.6 times compared with those of their controls. In the case of the effluent of the secondary treated sewage, the cell numbers of most species and strains of phytoplankton tested increased at both the early logarithmic and the stationary phase. S. costatum and Olisthodiscus sp., which were isolated from Dokai Bay. were more tolerant of higher concentrations of matters contained in the three industrial effluents than were the other three species and strains.
  • 山田 真知子, 武居 薫, 鶴田 新生
    1982 年 48 巻 10 号 p. 1457-1461
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Dokai Bay (Kitayushu City, Japan) receives the effluents from two different chromate processes of steel, an expensive-nonferrous-metal recovery process of iron sulfide, and an inorganic manure-producing process: these are the main sources of inorganic pollutants.
    Among five phytoplankton tested, the growths of the two species, Skeletonema costatum and Olisthodiscus sp., which cause the red tides in Dokai Bay were stimulated at the early logarithmic phase as well as the stationary phase by the addition of four kinds of effluents, compared with those of their controls. These two species were more tolerant of the inhibitory effects of the effluents than were the other three species and strains: Chaetoceros curvisetus which was isolated from Dokai Bay and S. costatum and Olisthodiscus sp. which were isolated elsewhere. These patterns of the growth response of the five species and strains of plankton to the effluents which mainly contained inorganics were similar to those patterns of the growth response to the the effluents which mainly contained orgaincs reported in previous paper.
    These results suggest that the growth specificities of the two kinds of plankton which caused the red tides in Dokai Bay to a variety of industrial effluents discharged into the bay could be sufficient to account for outbreaks of the red tide here.
  • Kok Leong WEE, Albert G. J. TACON
    1982 年 48 巻 10 号 p. 1463-1468
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    The dietary protein requirement of juvenile snakehead Channa micropeltes was in vestigated by feediung eight semipurified diets containing varying levels of dietary protein within a range of 25.5 to 56.5% by weight over a 56-day feeding period at 28°C.
    On the basis of daily weight gain and daily protein deposition these results indicate that the requirement by juvenile snakehead for dietary protein was about 52% of protein in the diet when fish meal is used as the sole protein source. The relationship between dietary protein level and food conversion ratio, protein efficiency ratio, apparent net protein utilisation, apparent protein digesti-bility, apparent dry matter digestibility, hepatosomatic index, and gross carcass composition were determined and discussed in relation to other fish species.
  • 杉田 治男, 店網 秀男, 出口 吉昭
    1982 年 48 巻 10 号 p. 1469-1471
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Sediments collected in the Edo river mouth, a polluted area, were quantitatively diluted by seawater diluent, smeated on glass slides, and Gram stained. Under a light microscope at 1500 magnigficaion, bacterial cells with a definite boundary were counted and divided into 4 groups on the basis of cell morphology and Gram reaction. Also, diluted sediments were inoculated onto PYBG and 1/20 PYBG agar plates, and incubated at 20°C for 5 days under aerobic and anaeribic conditions.
    Total direct counts ranged from 108 to 109g-1 in the sediments; Gram-negative rods were dominant. 60-70% of the bacteria observed were attached to organic matter. Total viable counts were 105 to 107g-1, which were 0.03 to 2.1% of the total direct counts. Major components were aerobic and facultatively anaerobic Gram-negative rods and bacilli.
  • 門谷 茂, 岡市 友利
    1982 年 48 巻 10 号 p. 1473-1479
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Organic carbon, nitrogen, and iron (total-Fe, reactive-Fe) in sedinents and iron in pore water were determined, using 23 sediment samples collected at the Harima-Nada, Seto Inland Sea, Japan.
    Vertical profiles of organic-C and reactive-Fe were similar and a high correlation coefficient (r=0.86) was detected between organic-C and reactive-Fe. A relatively high correlation coe-fficient (r=0.66) was also obtained between reactive-Fe in sediments and total-Fe in pore water. These results suggest that when a reductive condition is achieved in marine sedinents, some Parts of iron conjugated with organic matter are eluted to the pore water and subsequently returned to the overlying sea water.
    Orgnic iron in pore water of the sediments was separated with Sephdex G-10 chromatography. The growth-promoting activities to Chattonella aniqua with sepatated iron fractions in pore water were estimated. Considerable activites were found in the two iron fractions which eluted before the NaCl fraction.
  • 安元 健, 中村 宗知, 大島 泰克, 高畑 寿太郎
    1982 年 48 巻 10 号 p. 1481-1483
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Based on the fact that tetrodotoxin yields fluorescent 2-amino-quinazoline derivatives by alkali treatment, a continuous tetrodotoxin analyzer was constructed. The toxin was first separated from contaminants on a column of a cation exchange gel with a citrate buffer system. The eluate was mixed with an equal volume of 4 N sodium hydroxide and the toxin was converted to fluorescent compounds while passing through Teflon tubing immersed in a boiling water bath. Both the retention time of the toxin and the fluorescence intensity were recorded automatically by a fluoromonitor. The toxicity-fluorescence response showed good linearity in a wide range between 0.1 and 20 mouse units. The results were reproducible within a variation of 3%.
  • 岡野 正義, 深宮 斉彦, 荒谷 孝昭
    1982 年 48 巻 10 号 p. 1485-1490
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Polar constituents of the green alga Cladophora densa HARVEY, which grows in the tidal zone of the Seto Inland Sea, were identified by GC, HPLC, IR, 1H NMR, MS, synthesis, and chemical transformations to known compounds.
    The methanol extract of the dried algae, from which nonpolar constituents were removed beforehand by n-hexane extraction, consisted of large amouts of monoglycerides with C16-and C18-unsaturated fatty acids and L-alanine, and small amounts of glycerol, L-phenylalanine, Ltyrosine, 2, 3, 5, 6-tetraiodo-DL-tyrosine, and galactoside of β-sitosterol.
  • 松野 隆男, 勝山 政明
    1982 年 48 巻 10 号 p. 1491-1493
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    An attempt was made to determine whether or not Tilapia was able to metabolize zeaxanthin to its principal carotenoid, rhodoxanthin.
    Test diets containing zeaxanthin were fed to Tilapia for 30 days.
    The control group and the zeaxanthin fed group were similar to one another in the carotenoid patterns, but were different in the percentage compositions and the amounts of carotenoids, In the zeaxanthin group, rhodoxanthin accumulated and amounted to four times that of the control. It was concluded that zeaxanthin was metabolized to rhodoxanthin in vivo.
  • 鈴木 讓, 中原 元和, 中村 良一, 上田 泰司
    1982 年 48 巻 10 号 p. 1495-1500
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
    Radiocobalt tracer experiments were carried out to obtain further information on the biochemical behavior of this nuclide in sea cucumber and prawn. The biological half lives of 60Co (inorganic cobalt in sea water) were calculated at 73 days for sea cucumber and 24 days for prawn. The 60Co-binding constituents in the organs and tissues of these organisms were extracted with a Tris-acetate buffer solution and separated using gel filtration on Sephadex G-75. Although most radioactivity (86%) in the crude extraction of sea cucumber (without intestine)was found in a relatively high molecular weight fraction (more than 80000), that in prawn liver was observed in a low molecular weight fraction (less than 5000). From analysis for carbohydrates, a large portion of carbohydrates in the prawn liver was found in the high molecular fraction, however, the presence was observed in the low molecular weight fraction, too. Further characterization by electrophoresis showed that this fraction contained at least four 60Co-binding proteins or peptides and significant quantities of 60Co-binding carbohydrates.
    The biological role and detailed chemical nature of the components await further study.
  • 牧之段 保夫, 豊原 治彦, 池田 静徳
    1982 年 48 巻 10 号 p. 1501
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 小長谷 史郎
    1982 年 48 巻 10 号 p. 1503
    発行日: 1982/10/25
    公開日: 2008/02/29
    ジャーナル フリー
feedback
Top