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Volume 49, Issue 1
Displaying 1-26 of 26 articles from this issue
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Osamu IMADA1983 Volume 49 Issue 1 Pages 1-5
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPhotometer was invented to measure light intensity for photosynthesis in culture vessel of hydrophyte, mainly laver Porphyra sp. The photometer was composed of Cd-S photo-sensor covered by photospersion medium (in practice, milk white polyethylene glass) in order to ger uniform electromotive force caused by all direction light in aquatic system. Lead line from Cd-S photo-sensor was connected to lead cable, which were connected to micro-ampere meter and recorder. The photometer could be used under 5m depth of water, and applied to measure artificial and field culture condition of laver. Differences of photo-conditions; in or out of water, upper or lower site of culture net were determined on field culture of laver.View full abstractDownload PDF (496K) -
Hiromichi IMABAYASHI1983 Volume 49 Issue 1 Pages 7-15
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSEffects of diminished dissolved oxygen supply on the benthic communities were studied in eutrophicated Hiuchi-Nada and Harima-Nada of the Seto Inland Sea, where stratification was formed in summer, 1981.
The dissolved oxygen concentration was more markedly diminished at the bottom-layer, located 0.5 meter above the soft muddy sediment, in late summer than in early summer, but not so above the other sediments. Most of the benthos, found commonly in the bays or coastal waters of Japan, were molluscs and polychaetes with Theora lubrica and Paraprionospio sp. dominating, respectively.
Species composition of the benthic communities didn't indicate a striking change during the summer. On the other hand, the number of species and individuals decreased logarithmically with depletion of ambient dissolved oxygen, and also the species diversity decreased gradually. This was due to a decline in number of the small bivalve Theora lubrica, inhabiting the extreme surface sediment.View full abstractDownload PDF (606K) -
Takashi AOKI, Tadatoshi KITAO, Naoto IEMURA, Yasutami MITOMA, Tetsuich ...1983 Volume 49 Issue 1 Pages 17-22
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOne hundred and seventy-five strains of Aeromons salmonicida isolated in cultured salmonid fish and wild salmonid fish from rivers in Japan from 1979 to 1981 were tested for theri susceptibility to 12 chemotherapeutics. Five of 129 (3.9%) strains isolated in the cultured fish were sensitive to all the drugs tested. The remaining 124 strains were resistant to from one to six drugs; in particular, nalidixic acid derivatives and/or nitrofuran derivatives. Transferable R plasmids were detected in only 2 out of the 124 resistant strains. The resistant marker of the detected R plasmids determined as a combination of chloramphenicol (CM), streptomycin and sulfonamides (SA). The molecular weight of the R plasmids was 29M daltons. Non-transferable R plasmids resistant to TC were detected in two A. salmonicida strains habouring transferable R plasmid and had a molecular weight of 7.6M daltons.
On the other hand, 32 strains (69.6%) isolated in wild salmonid fish were sensitive to all the drugs tested. The remaining strains were resistant to SA, and to a combination of CM, colistin and SA. These resistant strains had no transferable R plasmids.View full abstractDownload PDF (1381K) -
Shin OIKAWA, Yasuo ITAZAWA1983 Volume 49 Issue 1 Pages 23-26
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTechniques for manometric determination of the rate of tissue respiration were examined with various tissues of carp, focussing on substrate solution, oxygen pressure of gas phase, and method of preparing tissue. Physiological saline was suffcient as the solution for a short period determination. Pyruvate, fumarate, glutamate, glucose, or calcium were not necessary to add to the solution for the manometry of 30min. Ordinary air (PO2=155mmHg) gave, compared with 3% oxygen (PO2=22mmHg), higher values for hepatopancreas and ordinary muscle, and lower values for blood, while roughly same values for testis and ovary. Homogenized and pestled tissues gave much lower values than scissored or intact ones for various tissues. Scissored preparation made by scissoring tissue in a chilled vessel about 400 times until it becomes pasty is considered to give the most reliable results for various tissues.View full abstractDownload PDF (306K) -
Wann-Nian TZENG, Osame TABETA1983 Volume 49 Issue 1 Pages 27-32
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSForty-two elvers of the short-finned eel Anguilla bicolor pacifica SCHMIDT were found among 1, 915 anguillid elvers caught from the estuaries in southern and northern Taiwan druing November 1980-April 1981. The pigmentation, body measurements and vertebral counts were examined. The short distance of ano-dorsal length and lower counts of ano-dorsal vertebrae distinguish them from elvers of A. japonica and A. marmorata, two other species commonly found in Taiwan. This first record from Taiwan extends the northernmost distribution of this subspecies.View full abstractDownload PDF (601K) -
Mitsuhiro NAGATA1983 Volume 49 Issue 1 Pages 33-40
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSEnergy transformations through the population of rocky intertidal herbivores, Strongylocentrotus intermedius, Chlorostoma argyrostoma turbinatum and Omphalius rusticus, were analyzed from Oct. 1978 to Oct. 1979, using estimates of average weight and numbers of individuals in each size-group and observations on respiratory rates. The energy entering the population, which is the sum of the energy assimilated as food and the rate of immigrants, was 118.4 Kcal m-2 yr-1 for the Strongylocentrotus population, 12.6 Kcal m-2 yr-1 for the Chlorostoma population, and 6.6 Kcal m-2 yr-1 for the Omphalius population. The amount of energy lost from the population, expressed as respiration, gametes ejected, predation, mortality and emigration, was estimated at 119.6, 13.4 and 6.5 Kcal m-2 yr-1 for the Strongylocentrotus, Chlorostoma and Omphalius populations, respectively.View full abstractDownload PDF (831K) -
Kohei KIHARA1983 Volume 49 Issue 1 Pages 41-47
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe recurrent groups were analyzed in order to clarify the mean pattern of the benthic species communities in the eastern Bering Sea druing the summers from 1966 to 1977 excluding 1972 and to elucidate the variations of these communities during the summers from 1966 to 1971 excluding 1969. The mutual affinities between L. aspera, T. chalcogramma, G. macrocephalus and P. quadrituberculatus were great and stable through these summers of five years. These species groups were defined as the core species group of the benthic species communities in the central region of this continental shelf. The structures of these communities are considered to be formed and to change according to the stable and the unstable relations between the species and the core species group.View full abstractDownload PDF (475K) -
Kohei KIHARA1983 Volume 49 Issue 1 Pages 49-54
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSInfluences of water temperature and salinity on the bottom layer upon the formation of the benthic species community were elucidated by analyzing fluctuations of the abundance, the affinity, the biomass diversity and the recurrent group of the benthic species in the eastern Bering Sea through the summers from 1966 to 1971 excluding 1969. Benthic species communities are supposed to be formed by these mutual relations between the stable core species and the unstable species to the environment such as A. stomias. The Alaskan Stream Extension Water and teh Shelf Water were recognized to have significant effects on this process.View full abstractDownload PDF (500K) -
Gunzo KAWAMURA, Takashi SHIMOWADA1983 Volume 49 Issue 1 Pages 55-60
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThis study was conducted to examine the ability of Japanese parrotfish Oplegnathus fasciatus (TEMMINCK and SCHLEGEL) to discriminate their vertically striped body coloration which could be a visual signal for intraspecific communication. The fish were trained to discriminate a shape which resembles Japanese parrotfish in countour and stripe coloration. After the training, transfer tests were run. The fish were not able to discriminate between shapes having five and seven vertical stripes. The difference between the two shapes employed was the presence of the 6th and 7th vertical stripes in the caudal area. The fish trained on the square shape having vertival stripes or horizontal stripes gave no evidence fo having learned to descriminate the exact number of stripes on each nor of having developed the higher ability to discriminate vertical stripes from horizontal ones. The ability of the fish to discriminate striped shapes by photopic vision was the same as that by scotopic vision at 0.01-0.03lx. From the results obtained, it was concluded that theri body coloration can be an intraspecific visual signal but the 6th and 7th vertical stripes might not be important for their social communication.View full abstractDownload PDF (414K) -
Kiyoshi ASAHINA, Isao HANYU1983 Volume 49 Issue 1 Pages 61-67
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe rose bitterling (Rhodeus ocellatus ocellatus, Cyprinidae), a spring and summer breeder, was exposed to various temperature and photoperiod regimes during the different phases of the annual reproductive cycle. In early spring, warm temperature stimulated the recrudescence of the gonads regardless of photoperiod. But during late summer and autumn, a long photoperiod was required to maintain or initiate the gonadal maturation especially at high temperatures (22-28°C). These results indicate that the initiating factor of the breeding season in this species is a rising temperature, whereas the terminating one is the decreasing daylength at high temperatures. Therefore, in the rose bitterling, the responsiveness of the gonads to photoperiod varies clearly with season. The critical photoperiod for maturation falls between 13 and 14hours of light per day. This critical photoperiod could be changed by a pretreatment of artificial photoperiod during the breeding season.View full abstractDownload PDF (545K) -
Masashi TSUKAMOTO, Takayuki ONOHARA, Keizo USHIROGAWA, Shuhei MATSUURA1983 Volume 49 Issue 1 Pages 69-74
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe mud skipper Boleophthalmus chinensisi is an amphibious fish. In Japan, its distribution is limited only to the inner part of the Ariake Sea and a part of Yatsushiro Bay.
The present study was aimed at selecting the most suitable age character for age determina-tion of this species from among the pectoral radials, coracoid, pelvic girdle, scale, otolith, and vertebrae.
The marks of the pectoral radials, coracoid, and pelvic girdle were prominent enough to make age determination, while those of the scale, otolith, and vertebrae were obscure. Among the former three characters, the center of mark-forming (focus) was observable most clearly in the size and pectoral radials, particularly in the 2nd one.
All eight pectoral radials from same individual showed good correlation between their mark-size and their radial-size.
The relationship between the 2nd pectoral radial-size (R: mm) and the total length (TL: mm) was expressed by the following equation;
R=0.023 TL-0.135 r=0.987.
Therefore, the 2nd pectoral radial was the most suitable age character of the mud skipper.View full abstractDownload PDF (592K) -
Akira SHINAGAWA, Kazuo SHIOMI, Hideaki YAMANAKA, Takeaki KIKUCHI1983 Volume 49 Issue 1 Pages 75-78
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSSelective determination of inorganic arsenic (III), (V) and organic arsenic by the solvent extraction method was done with 20 species of marine organisms, including fish, ascidian, holo-thurian, sea urchin, crustacean, shellfish, cephalopod, polychaete, and seweed. In all species, inorganic arsenic, which is more highly toxic than organic arsenic, comprised only a small part (0-7%) of the total arsenic; the one exception was in a seaweed Hizikia fusiforme, in which inorganic arsenic accounted for 60% of the total arsenic. Furthermore, when arsenic in water-soluble and fat-soluble fractions was determined, the ratio of water-soluble arsenic reached 58-97% of the total arsenic. Especially in the cephalopod (3 species) and fish (6 species), more than 84% of the total arsenic was found in the water-soluble fraction. These results strongly suggested that most arsenicals in marine organisms are present in water-soluble organic forms.View full abstractDownload PDF (292K) -
Kazuo SHIOMI, Akira SHINAGAWA, Hideaki YAMANAKA, Takeaki KIKUCHI1983 Volume 49 Issue 1 Pages 79-83
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe major arsenic compound in the muscle of an octopus Paroctopus dofleini was purified by column chromatographies on ion-exchangers and Bio-Gel P-2. The arsenic content of the purified compound was estimated to be 44%; this is close to that (41%) of arsenobetaine. In thin layer chromatography and electrophoresis, the purified arsenic compound gave a single spot positive to the Dragendorff reagent at the same Rf and mobility as arsenobetaine. No characteristic UV absorption was observed with the purified compound. The 1H-NMR spectrum of the purified compound showed two significant signals at δ 1.88 (s, 9H) and 3.31 (s, 2H), which corresponded to those at δ 1.87 (s, (CH3)3 AS) and 3.28 (s, CH2) reported for arsenobetanine. No difference was found between the IR spectra of the purified compound and arsenobetaine. Judging from these results, the major arsenic compound in the muscle of P. dofleini was concluded to be arsenobetaine (CH3)3AS+CH2COO-. Moreover, arsenobetaine accounted for more than 90% of the total arsenic in P. dofleini.View full abstractDownload PDF (329K) -
Hiroshi NIKI, Tsuneo WADA, Seiichiro IGARASHI1983 Volume 49 Issue 1 Pages 85-89
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn producing Active Fish Prothein Powder (AFPP) by spray-drying, one of the most impor-tant problems is the recovery of fish meat powder.
It was presumed that the recovery of AFPP is affected by its moisture content and moisture is affected by the viscosity of fish meat sol and the temperature of spray-drying.
Accordingly, the influence that the viscosity of fish meat sol and inlet and outlet air tempera-ture of spray-drying exert on the recovery, the moisture, and the particle size of AFPP were researched.
The recovery of AFPP was related to its moisture content and increased under the condition that the moisture of AFPP is reduced. In order to reduce the moisture of AFPP, the lower viscosity of fish meat sol, the lower inlet air temperature, and the raised outlet air temperature were needed.
The particle size of AFPP was affected by the viscosity of fish meat sol. The high viscosity allowed big drops of fish meat sol to grow on spraying and produced big particles of AFPP. The big drops collided with the wall and the bottom of drying-chamber directly and caused the recovery of AFPP to decrease.View full abstractDownload PDF (444K) -
Hiroshi NIKI, Tsuneo KATO, Eiki DEYA, Seiichiro IGARASHI1983 Volume 49 Issue 1 Pages 91-96
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSActive Fish Protein Powder (AFPP) produced by spray drying has gel-forming, water holding and emulsifying properties that are useful for foodstuffs.
The water holding capacity and emulsifying capacity of AFPP were influenced by pH and fish meat content. AFPP was able to hold 40 grams of water per one gram of fish meat (centri-fugal method (10, 000xg)) and to emulsify 180 ml of oil per one gram of fish meat (continuous emulsifying method).
The water holding capacity was related to the kamaboko-forming property (gel strength and bending test) of AFPP. This can be a criterion for judging the quality of AFPP.
A feature of AFPP is its convenience for transport and storage. AFPP maintained its capacities for 6 months at 5°C, but lost them rapidly at 30°C. The low moisture content of AFPP and the elimination of oxygen from the package prevented the deterioration of its quality during storage.
In order to increase the storage ability of AFPP, it was necessary to make low moisture AFPP and to store at low temperature and under deoxygenating.View full abstractDownload PDF (377K) -
Keijiro SEZAKI, Shugo WATABE, Kanehisa HASHIMOTO1983 Volume 49 Issue 1 Pages 97-101
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe diploid and triploid populations of “ginbuna” Carassius auratus langsdorfi were com-pared in terms of several consituents in the muscle and blood. The muscles of both populations did not differ clearly from each other in the proximate and protein compositions. No significant differences were found in the hematocrit value and hemoglobin level of the blood either.
Activities of some erythrocyte glycolytic enzymes such as hexokinase and phosphofructokinase were 1.26-1.35 times higher in the triploid than in the diploid, on a per cell basis. However, the activities of each enzyme matched well each other on a unit blood volume basis. This, together with other results, suggested that a regulation system is retained in the triploid so that its metabolic rate is controlled at the diploid level.View full abstractDownload PDF (588K) -
Keishichi YOKOYA1983 Volume 49 Issue 1 Pages 103-107
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDried pieces of natural, cultured and force cultured “makombu” Laminaria japonica were dipped for a certain time in distilled water (30°C), amino acid and acids, and their rehydrated volume and rate constant as well as texture in boiling at 90±2°C were examined.
By rehydration with distilled water per gram in equilibrium (dry matter), 4.45g of water was absorbed by natural, 4.21g by cultured, and 4.45g by force cultured “makombu”, the values were somewhat dependent on growth. In amino acid solutions absorption was 4.32-4.45g in alanine and 4.27-4.35g in glycine, showing a trend of swelling in the rehydrated texture. In acid solutions, absorption was 1.60-1.67g in acetic acid and 2.03-2.23g in lactic acid, these values were lower than that of amino acid solutions, but marked softening of texture was noted.
Rehydration rate constants were within a range of 4.14-8.29×10-2(min-1) in distilled water, 2.99-6.44×10-2 (min-1) in amino acid solutions and 4.60-9.45×10-2 (min-1) in acetic acid and lactic acid, each of such K values had the highest value for the force cultured “makombu”. Meanwhile, reproduction of texture after rehydration was favorable and hardness after rehydra-tion showed nearly a linear increase up to 20 minutes. After that, it held the characteristic hardness in the respective acid solution. In acetic acid, a decreasing trend was notableView full abstractDownload PDF (407K) -
Yasuo MAKINODAN, Haruhiko TOYOHARA, Shizunori IKEDA1983 Volume 49 Issue 1 Pages 109-112
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe existence of acid, neutral, and alkaline proteinases in fish muscle was examined. The species used were common mackerel, sardine, horse mackerel, white croaker, lizardfish, and deep-sea fish such as black halibut, soft eelpout, pectral rattail, and threadfin hakeling. Each proteinase activity was recognized in all fish ested. Specific activity of acid and neutral proteinases was large in the deep-sea fish, but tissue activity was small. The alkaline proteinase activity was recognized also in common mackerel and sardine, though the activity had not been detected previously in these fish. There was a fair difference among species in this specific activity; especially in sardines the activity was very large.View full abstractDownload PDF (245K) -
Tooru OOIZUMI, Eiko USUKI, Kazuo YABE, Ken-ichi ARAI1983 Volume 49 Issue 1 Pages 113-121
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA change in the muscular protein of Antarctic krill with its digestive protease of hepatopancreas has been studied by measuring the viscosity of actomyosin. Taking account of the instability of actomyosin, the decrease in viscosity was assayed in the presence and absence of sorbitol. The decrease in viscosity was thus found to proceed in two distinct stages: a fast first-order decrease in an early stage, followed by a relatively slow first-order decrease. The first-order rate constant (K') at the early stage was then used as a measure of digestibility of actomyosin.
In the presence of 1.5 M sorbitol, actomyosin was digested with various concentrations of crude protease and at various temperatures. It was thus found that actomyosin of krill was several times more rapidly digestible than that of carp was. When the digestion was performed in the presence of various concentrations of sorbitol (0-2.5 M), the rate constant (K') was found to decrease with increasing molarity of sorbitol. From the linear relation between log K' and sorbitol (M), the K' value for actomyosin of krill without sorbitol was theoretically obtained.
SDS-gel electrohporetic patterns of these digests showed that myosin heavy chain of krill was rapidly decomposed by its digestive protease, but this decomposition was effectively prevented by sorbitol.View full abstractDownload PDF (908K) -
Toshiaki OHSHIMA, Shun WADA, Chiaki KOIZUMI1983 Volume 49 Issue 1 Pages 123-130
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPhosphatidylcholine (PC) and phosphatidylethanolamine (PE) of cod Gadus morhua were spearated by HPLC and analyzed for the fatty acid compositions and the compositions based on total carbon in fatty acyl chains by GLC. From the results obtained, the possible fatty acid combinations of PC and PE were estimated.
Without regard to the number of double bonds in the fatty acid, eight possible fatty acid combinations: (C18, C22), (C18, C20), (C18, C18), (C20, C20), (C20, C22), (C18, C22), (C22, C22), and (C14, C20), were estimated for the PC. The combination of (C16, C22), was most prominent, acounting for 44.2% in the PC. Similarly, nine possible fatty acid combinations: (C18, C22), (C16, C22), (C20, C20), (C20, C22), (C18, C20), (C22, C22), (C14, C22) (C16, C20), and (C18, C18), were estimated for PE. The combination of (C18, C22) was most prominet, accounting for 23.5% in the PE.
From the fatty acid compositions, the major fatty acid combinations of PC and PE were tentatively estimated to be as follows: (C16:0, C22:6), (C16:0, C20:5), (C18:1, C18:1), (C20:5, C20:5), (C20:5, C22:6), (C18:1, C22:6), (C22:6, C22:6), and (C14:0, C20:5) for PC and (C18:1, C22:6), (C16:0, C22:6), (C20:5, C20:5), (C20:5, C22:6), (C18:1, C20:5), (C22:6, C22:6), (C14:0, C22:6), (C16:0, C20:5), and (C18:1, C18:1) for PE.View full abstractDownload PDF (556K) -
Atsushi MOCHIZUKI, Masahiro MATSUMIYA1983 Volume 49 Issue 1 Pages 131-135
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSLysozyme distributions in the shellfishes Tapes philippinarum, Meretrix lusoria, Corbiculina leana, Crassostrea gigas, Nordotis discus, scapharca broughtnii, Mytilus edulis, Anodonta japonica, Cristaria plicata, Charonia sauliae and Batillus cornutus were investigated.
Lysozyme was chromatographed on a chitin-coated cellulose column. The enzyme activity was assayed with Micrococcus lysodeikticus cells and by the ferricyanide method with glycol chitin as substrate.
Lysozyme was found in the liver and muscle of 5 shellfish species and in the gill and mucus of 4 species. High lysozyme activity was found in the whole body of M. edulis and C. plicata. The C. leana lysozyme showed maximum activity at pH 4.5 and 65°C. The enzyme remained active for 30 min up to 90°C at pH 4.8. At temperatures above 90°C, the enzyme was completely inactive. The activity of C. leana lysozyme was inhibited by incubation with Fe2+, Fe3+, Mn2+, Hg2+, Cd2+, Cu2+ and Ni2+ but slightly enhanced by Mg2+, Ba2+ and Zn2+.View full abstractDownload PDF (370K) -
Takumi SOEJIMA, Kenneth L. SIMPSON, Teruhisa KATAYAMA1983 Volume 49 Issue 1 Pages 137-139
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAustralian Artemia (Shark Bay) were fed rice bran for 10 days, then fed purified astacene 6 days, then returned to rice bran for 6 days. Artemia were shown to be able to bioaccumulate astacene to the same extent as astaxanthin. While the occurrence of astacene may be the result of oxidation during the analysis, these results show that its occurrence may also be the result of bio-accumulation. No other pigments than astacene, echinenone and canthaxanthin were isolated from Artemia fed astacene.View full abstractDownload PDF (237K) -
Satoshi HIKICHI, Takao OJIMA, Satoru KAKUDATE, Kiyoyoshi NISHITA1983 Volume 49 Issue 1 Pages 141-148
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSMyosins were successfully isolated from the smooth muscles of Akazara adductor and surf-clam foot by the method of EBASHI, which has been used for preparation of chichen gizzard myosin.
The properties of ATPase activity of these myosins were similar to those of Akazara striated adductor myosin and of rabbit skeletal myosin, except for the specific activity level and its KCl concentration dependence.
Judging from the SDS-and urea-gel electrophoretic patterns, Akazara smooth muscle myosin was shown to contain two different kinds of regulatory light chains (so-called EDTA-LC) besides heavy chain and essential light chain (so-called SH-LC), while surf-clam foot muscle myosin was found to contain only one kind of regulatory light chain, as other muscle myosins do.
The regulatory light chains can be reversibly and completely removed from both smooth muscle myosins by washing with 10mM EDTA at 0-4°C, resulting in complete loss of Ca2+ -sensitivity of Mg-ATPase activity and of superprecipitaion ability. From these results, both smooth muscle myosins appeared to be grouped into the same type of Akazara and scallop striated adductor myosins, whose regulatory light chains are readily removable.View full abstractDownload PDF (3414K) -
Keiko YOSHIOKA1983 Volume 49 Issue 1 Pages 149
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (113K) -
Keiko YOSHIOKA, Manabu KITAMIKADO1983 Volume 49 Issue 1 Pages 151
Published: January 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (1071K) -
1983 Volume 49 Issue 1 Pages 156
Published: 1983
Released on J-STAGE: April 22, 2008
JOURNAL FREE ACCESSDownload PDF (56K)
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