日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
49 巻, 11 号
選択された号の論文の25件中1~25を表示しています
  • 原 一郎
    1983 年 49 巻 11 号 p. 1619-1625
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The method of integral geometry is developed to estimate fish density. We can estimate two dimensional measure using measurement of one dimensional quantity. In this papar, we apply this method called line intercept method. The conditions using this method are as follows: searching individuals by observer, numrous readily visible individuals, large individuals and interception of object by line. In this research, it was recognized that the use of an echo sounder as a searching observer is good for the line intercept method. There are some problems in estimating biomass, but the method is a good way to estimate number of fish schools. We think this method may be used widely in the future, though it has not yet been put sufficiently into practical use in the field of fisheries.
  • 工藤 重治, 木村 紀彦
    1983 年 49 巻 11 号 p. 1627-1633
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The process of recovery from bacterial gill disease, which is characterized by a hyperplasia of the gill epithelium, was examined by transmission and scanning electron microscopy. An attempt to effect recovery from the disease was made by immersing naturally infected rainbow trout fingerlings for 1min in a 5% solution of sodium chloride. This treatment was very effective for the removal of bacterial cells and subsequent recovery. The removal resulted in the degeneration of epithelial and chloride cells in the outermost layer and the subsequent breakdown or exfoliation from the surface of the hyperplastic epithelium. In the superficial layers of epithelial cells in hyperplastic lesions, electron lucent or pale granules (275nm in average diameter) appeared singly or in groups; these are formed by Golgi complexes. The opening of the granules may accelerate the exfoliation of degenerating cells from the hyperplastic epithelium. These processes resulted in the complete recovery of the gill epithelium. A cytoplasmic “cutting-away” or “fragmentation” of epithelial and chloride cells was observed in the deep interior of the lesions.
  • 工藤 重治, 木村 紀彦
    1983 年 49 巻 11 号 p. 1635-1641
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The process of the recovery from bacterial gill disease induced by an artificial infection was examined by transmission and scanning electron microscopy. Artificial infection was accomplished by immersing healthy trout fingerlings for 60min in a bacterial suspension. Five days later, the diseased fingerlings were immersed for one minute in a 5% solution of sodium chloride, followed by fixation at intervals of 2h, 1, 2, 3, 4, and 5 weeks. Bacterial gill disease induced by the artificial infection was characterized by a hyperplasia similar to that due to a natural infection. Epithelial and chloride cells, which were enclosed in the interior of the lesions by the fusion of gill lamellae, showed a cytoplasmic “cutting-away” or “fragmentation” at the cell perphery. This phenomenon was most striking in chloride cells, but was not found in mucous cells. One week after the salt solution treatment, epithelial and chloride cells in the outermost layer of the lesions had begun to degenerate or break down and then exfoliate. The increase of lysosomes was striking in the enclosed cells. In the superficial layers of epithelial cells, electron lucent or pale granules (275nm in average diameter) appeared in the cytoplasm. The granule formation and subsequent opening were most striking in cases two weeks after the salt solution treatment, thus the opening may help to accelerate healing of the lesions. With the passing of time, the hyperplastic lesions progressively healed and complete recovery was seen five weeks after the salt solution treatment. The present experiments reaffirmed that the bacterium which we have isolated is a causative agent of bacterial gill disease.
  • 安藤 芳明, 砂川 紘之, 都築 俊文, 大石 圭一
    1983 年 49 巻 11 号 p. 1643-1648
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    Since the role of Thermoactinomyces vulgaris in the etiology of the so-called “Kombu asthma” breaking out among fishermen engaging in Kombu production was strongly suspected, its occurrence and distribution in the free environment of the fishing area of Hakodate city were studied. Mud, sand, soil, water, and Kombu (Laminaria japonica) were collected from sea shore, its tributary rivers, and from land areas around sea. The results are summerized as follows:
    1) Thermoactinomyces sp. was detected in the surface of freshly harvested Kombu, in particular, was most abundant in parts of the blade infested with a kind of Bryozoa (“Kokemushi”), but was scarce in normal or some other parts infested with a kind of Hydrozoa (“Hige”).
    2) All of 8 samples of sea water and 17 of 25 samples of sea sand showed the presence of Thermoactnomyces sp. spores, viable counts being 5-410 spores/l and 5-47 spores/g dry wt, respectively.
    3) All of 14 samples of river water and 25 of 30 samples of river mud collected at different sampling stations on the four rivers had 3-1000 spores/l and 7-1800 spores/g dry wt, respectively.
    4) All of 14 soil samples taken from the places of composts gave relatively high spore numbers of Thermoactinomyces sp.; 4 of the soil samples had as many as 106 spores/g dry wt and the remaining 10 samples had over 200 spores/g dry wt.
    Therefore, it appears that the major source of Thermoactinomyces sp. in the sea area where Kombu blades grow is the organisms being washed-in from the surrounding land areas.
  • 北川 大二, 長洞 幸夫
    1983 年 49 巻 11 号 p. 1649-1654
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    Physiculus maximowiczi is common in the coastal waters of Iwate Prefecture, northeastern Japan. A total of 1274 individuals were collected from the continental shelf at a depth of 50m to 180m from 1976 to 1980 and were examined for length frequency distribution, sex ratio and GSI. The mean body length of females was significantly larger (P<0.05) than that of males through the year. The mean body length increased in the summer and exceeded 300mm and 270mm in femles and males, respectively. It decreased markedly in November. Females outnumbered males, and the total sex ratio was 19.4%. The ratio increased during the summer and decreased in November. The GSI increased from September to November and remained at a low level from December to August. But there were no mature ovaries during the year. Comparison of the GSI from two samples collected from 130m and 330m in depth during November and December, respectively, showed that the ovaries of fish from 330m were more developed than those of fish from 130m. The present results may suggest that P. maximowiczi spawns during the winter in deeper water than the continental shelf.
  • 谷口 順彦, 関 伸吾, 稲田 善和
    1983 年 49 巻 11 号 p. 1655-1663
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The samples from 6 natural populations of amphidromous race collected in Kochi Prefecture, 2 from landlocked populations, and 2 artificially propagated populations of Ayu, Plecoglossus altivelis were used for examining isozyme markers in order to clarify genic variability and differentiation of populations. Among 20 loci examined, 14 loci were polymorphic in total samples. The genic variability was not low in comparsion with the other fish species, as shown in the number of alleles per locus ranging from 1.25 to 1.60, the proportion of polymorphic loci per population from 0.250 to 0.450, and heterozygosity from 0.034 to 0.090. The level of genic variability of landlocked form was lower than the amphidromous form. The allele frequency was very similar within a race as shown by the genetic distance (D=0.0001-0.0008 in amphidromous form, D=0.0003 in landlocked form). On the other hand, the genetic distance between amphidromous and landlocked forms was relatively large (D=0.0035). Distinct change was observed in the allele frequencies and genic variability in the hatchery population, possible effects of founder population and selection being suggested.
  • 手島 和之, 原 政子
    1983 年 49 巻 11 号 p. 1665-1668
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    Morphology of intestinal epithelial cells in the freshwater elasmobranch Potamotrygon magdalenae was studied by electron microscopy. Columnar cells lining the internal surface of the intestine as a single layer in P. magdalenae are characterized by microvilli densely arranged on the free surface, intermicrovillous invaginations, numerous vesicles beneath the microvilli and granules of varying sizes. These morphological characteristics suggest that columnar cells with microvilii function as absorptive cells. Interdigitations of the lateral plasma membranes, which are not found in teleosts, occur at junctions of adjacent cells in the P. magdalenae intestine. It is shown that these interdigitations exist not only in marine, but also in freshwater elasmobranchs.
  • 中村 薫
    1983 年 49 巻 11 号 p. 1669-1672
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The suboesophageal ganglion of the prawn Penaeus japonicus was composed of five neuropiles, locally distributed nerve cells, and dorsal axon bundles connecting with the superior and inferior nervous systems. The nerve cells held between giant axons were divided into two dorso-median groups at the surface of the posterior neuropiles. Procion yellow was injected antidromically into neurons connected with the midgut gland. The axons injected with the dye were traced to comparatively large cell bodies in the dorso-median groups. These neurons were derived from two different sites, i.e., two paris of the dorso-lateral and a few members of the dorso-median of the ganglion. A part of the ventral wall of anterior midgut gland received both distal ends of the neurons and a cord-like spongy body connected with the anterior of the ganglion. Little has been known on the routes connecting the midgut gland with the ganglion. These results would provide a possible existence of the motor function of the midgut gland.
  • 青木 宙, 竹下 淳, 北尾 忠利
    1983 年 49 巻 11 号 p. 1673-1677
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The susceptibility of various chemotherapeutic agents against five hundred and sixty one strains of non-hemolytic Streptococcus sp. were tested. These strains were collected from streptococcal infection of marine fish, yellowtail Seriola quinqueradiata, from 1974 to 1981 in various districts of Japan. The fifteen kinds of chemotherapeutics used were ampicillin (ABP), cephalexin (CEX), cephazoline (CEZ), chloramphenicol (CM), tetracycline, streptomycin (SM), kanamycin (KM), erythromycin (EM), leucomycin (LM), josamycin (JM), triacetyloleandomycin (TAO), lincomycin (LIM), nalidixic acid (NA), furazolidone (NF), and sulfamonomethoxine (SA). Four hundred and eleven strains isolated from 1978 to 1981 were additionally tested for sensitivity to doxycycline, trimethoprim (TMP), and ormethoprim (OMP), and one hundred and fifty nine strains isolated in 1981 were also tested for sensitivity to spiramycin (SP).
    EM was the most effective against the strains among the tested drugs, with all strains inhibited at a concentration of 0.1μg/ml or less. Successively, ABP, tetracycline derivatives, JM, TAO, and LIM had high antibacterial activity, and their MIC values for 90% of the strains were 1.24μg/ml or less.
    These strains were sensitive to CM, CEZ, KM, and SP, and moderately sensitive to CEX, SM, NF, TMP, and OMP. The strains of Streptococcus sp. were intrinsically resistant to NA and SA.
    Key words: yellowtail, fish disease, fish-pathogenic bacteria, streptococcal infection, non-hemolytic Streptococcus sp., drug susceptibility.
  • 小栗 幹郎
    1983 年 49 巻 11 号 p. 1679-1681
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    In comparison with normal rainbow trout, considerable reduction of number was observed in the dermal melanophores of its “cobalt” variant, which is devoid of normal pituitary gland and has only pituitary remnant, detached from or adherent to the base of the brain. Furthermore, in the “cobalt” variant remarkable reduction of number was detectable in the renal melanincontaining cells, which are abundantly present in the renal interstitial tissue of normal rainbow trout. This suggests that pituitary gland has an important role in the cell proliferation and melanin synthesis, not only in the dermal melanophores, but also in the renal melanin-containing cells of rainbow trout.
  • 阿部 宏喜
    1983 年 49 巻 11 号 p. 1683-1687
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    By high-performance liquid chromatography the distribution of L-histidine, carnosine, anserine, and balenine was examined in the white and red muscles of nine species of marine fishes. The L-histidine contents of white muscle were 15.8-92.8μmol/g in dark-fleshed fishes, 0.0734-0.931μmolg in white-fleshed fishes, and 4.06-20.3μmol/g in intermediate fishes. The L-histidien contents fo red muscle were lower than that of white muscle. Balenine as well as 1-and 3-methy ?? -L-histidine was detected only in a trace amount in a few species. In contrast, carnosine and anserine were found in the white and red muscles of almost all species, in which skipjack white muscle showed the highest contents of 2.46-5.57μmol/g (carnosine) and 7.69-21.2μmol/g (anserine).
    In skipjack tuan, L-histidine, carnosine, and anserine were distributed at somewhat higher levels in dorsal white muscle than in ventral muscle. The level of each compound differed significantly among the anterior, middle, and posterior portions of skipjack red muscle. In skipjack tuna, L-histidine was also detected in other tissues, such as intestine, stomach, pyloric caecum, with the highest level of 20.9μmol/g in pyloric caecum. Carnosine and anserine were also present in these tissues, but in smaller amounts.
  • Golam SARWAR, 坂田 泰造, 柿本 大壱
    1983 年 49 巻 11 号 p. 1689-1694
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The production and characteristics of carrageenan decomposing enzyme were studied for the cell free medium of a marine Cytophaga 1k-C783.
    Artificial sea water (ASW) components were necessary for enzyme production as well as bacterial growth. But glucose, galactose and cellobiose showed inhibitory effect for bacterial growth and enzyme production.
    Optimal activity was found at pH 7 and a temperature range from 25 to 50°C. However, above 50°C enzyme activity became unstable and it was totally inactivated by heat treatment at 55°C for 10min. It was also found that HgCl2, AgNO3 (1mM) and NaCl (1.5% to above) completely inhibited the enzyme activity.
  • 松宮 政弘, 大竹 茂夫
    1983 年 49 巻 11 号 p. 1695-1699
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The homogenates of the pyloric caeca and the intestine of common mackerel Scomber japonicus were fractionated by DEAE-cellulose column chromatography. The fractions exhibiting diamine oxidase activity were searched and some properties of the enzymes were examined.
    Three protein fractions were observed in both the chromatogram, and the diamine oxidase activities which were assayed by using histamine and cadaverine as the substrates which were recognized among the two.
    The follwing properties were identical in the two fractions from both the organs:
    The optimal pH values of the enzymes were at 7.3 and 8.0 when histamine and cadaverine were utilized as the substrates respectively. From the examination on the substrate specificity it was observed that the activities on histamine and cadaverien were higher than those on agmatine, and the highest activity was shown on cadaverine. The activities were inhibited slightly by EDTA and strongly by aminoguanidine.
    The properties of the diamine oxidase in the pyloric caeca and the intestine were indentical, but different from those of the liver enzyme in the fractionation patterns, optimal pH values and substrate specificities.
  • 田中 宗彦, 鈴木 康策, 田口 武
    1983 年 49 巻 11 号 p. 1701-1705
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    To recover proteins as a spun product from sardine viscera and heads for the use of food ingredient, factors affecting the extraction of proteins were investigated. Factors examined were extraction time, temperature, NaCl concentration, and effect of pH. The most appropriate extraction was made by water at 22°C for 30min at pH 10.5 (final value). In order to concentrate proteins, the extracted proteins were precipitated out by adjusting the pH to 5.0, and the precipitates were dissolved in NaOH solution for the preparation of spinning dope. The coagulation solution containing 20% NaCl or 20% CaCl2 in 1 N acetic acid gave 40-59% recovery of proteins in spun products. On the other hand, a spun product prepared by using 1 N acetic acid in ethanol brought about 70% protein recovery and its lipid content was fairly low.
  • 村上 正忠, 藤沢 浩明
    1983 年 49 巻 11 号 p. 1707-1715
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    In the bottom sediments of Hiro Bay, which is polluted with kraft pulp mill effluent, the distribution of heterotrophic bacteria and hemicellulose-decomposing bacteria, and the evironmental conditions were investigated five times from 1979 to 1981. The results on aerobic bacteria were as follows: The number of heterotrophic bacteria, glucomannan-decomposing bacteria, xylan-decomposing becteria and agar-decomposing bacterai per g of wet sediments were 104 to 107, 103 to 107, 103 to 105 and 102 to 104, respectively. The results on anaerobic bacteria were as follows: The number of heterotrophic bacteria, glucomannan-decomposing bacteria, xylan-decomposing bacteria and sulfate-reducing bacteria per g of wet sediments, were 104 to 106, 102 to 107, 108 to 106 and 103 to 107, respectively. The number of aerobic xylan-decomposing bacteria and sulfate-reducing bacteria in Hiro Bay were higher than those in the unpolluted control area (Yoshimi Bay), and the ratio of the number of hemicellulose-decomposing bacteria to that of heterotrophic bacteria in Hiro Bay were also high.
  • 荒木 繁, 小川 廣男, 大房 剛, 鹿山 光, 小林 彰夫
    1983 年 49 巻 11 号 p. 1717-1720
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The flavor of dried laver “Nori”, Porphyra yezoensis which was stored for 10 days under different water activities at 20°C, was studied by gas-liquid chromatography (GLC).
    By the head space gas analysis a single peak which was found on GLC equipped with the flame photometric detector, was identified as dimethyl sulfide (DMC).
    The amount of DMS production was dependent on water activity (aω); DMS was slightly detected at lower aω than 0.2, but abruptly increased above aω 0.3.
    Some parallelisms were found between DMS production and organoleptic evaluation, that is, while no flavor was felt at aω 0 or 0.1, off-flavor was recognized at higher aω than 0.4.
    Some peaks were also found on GLC through the flame ionization detector, and the changes of these GLC patterns were also related to water activity.
    From these results, it was suggested that the flavor of “Nori” can be preserved most effectively at aω below 0.1, and that its quality can be judged from the extent of DMS production.
  • 柴田 宣和, 尾崎 弘忠, 藤井 豊
    1983 年 49 巻 11 号 p. 1721-1729
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The muscle collected from fresh Antarctic krill Euphausia superba was stored at 2°C. The protein extracted with a medium of I=0.05 from the stored muscle was used to study the physicochemical and biochemical properties or elution profile in Sepharose C1-4B gel filtration of muscular protein.
    Extractability, viscosity, dependency of viscosity on protein concentration, ATP-sensitivity, flow birefringence and Mg-ATPase activity of muscular protein were low for the protein extracted from fresh muscle. The elution profile in gel filtration indicated that a large amount of the actomyosin extracted from fresh muscle had relatively small molecular size.
    The values of the above-mentioned physicochemical and biochemical properties became high with the lapse of storage time, reaching to a maximum within 2-3 days and then began to decrease.
    The Ca-ATPase and Mg-ATPase activities decreased very rapidly, whereas extractability and viscosity number were kept constant for a long time, and tended to decrease after 9 days of storage. The elutin profile of gel filtration of the extact from muscle stored for 9 days suggested that actomyosin of large molecular size occupied a large portion of the elute.
  • 上田 正, 武田 道夫
    1983 年 49 巻 11 号 p. 1731-1735
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    In 29 individuals of two species of sharks Mustelus griseus and Mustelus manazo levels of total mercury (T-Hg), methyl mercury (MeHg) and selenium (Se) in muscle tissue were determined. The range of T-Hg, MeHg and Se were as follows: 0.13-0.70, 0.11-0.68 and 0.17-0.27μg/g wet weight in Mustelus griseus, respectively: and 0.13-0.98, 0.12-0.88 and 0.21-0.46μg/g wet weight in Mustelus manazo, respectively.
    The correlation coefficient between T-Hg and length was positive and statistically significant in both species, while for Se insignificant correlation was observed. In Mustelus manzo, T-Hg depended closely on the age of the fish. The correlation coefficient between Se/Hg in molar basis and T-Hg was negative and statistically highly significant in both species. In embryo, both mercury levels were considerably low, while Se was nearly the same in comparison with those of the mother.
  • 杉田 治男, 出口 吉昭
    1983 年 49 巻 11 号 p. 1737-1740
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    Six culture media including 2 general and 4 selective agar media were examined to select the media for the investigation of aerobic microflora in aquatic environments. As criteria for the selection, the number of developed colonies, genus diversity of grown bacteria and viable counts of each genus on each medium was adopted using water, sediment and organism samples taken from the Tama River.
    In the course of this study it was found that (1) the composition of medium influenced not only viable counts but also generic composition of the bacteria grown on it; (2) A medium with lower nutrient (1/20 PYBGF agar) supported a vareity of bacteria with high population densities; (3) viable counts of each genus varied with the composition of media; (4) Many kinds of bacteria other than the target organisms were recovered on a selective medium. It is thus suggested from the above results that the combination of 1/20 PYBGF, PYBGF, MacConkey and PEA agars is suitable to isolate and investigate the aerobic microflora in freshwater environments.
  • 黒川 孝雄
    1983 年 49 巻 11 号 p. 1741-1746
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    A seasonal variation of the amounts of chemical components of sardine, Sardinops melanosticta, caught in the coast of Nagasaki Prefecture from January to May of 1981 and 1982 were investigated. The results were as follows.
    Wide seasonal varitions in the moisture and lipid contents of sardine were found, while the sum of percentages of moisture and lipid contents was nearly constant throughout the season, being 79.9±1.1% Correlation coefficient between moisture and lipid contents was -0.979.
    The seasonal variation in the contents of crude protein and crude ash is small, the value wes 16.7±1.2 and 3.3±0.3%, while the crude protein content in the sardine having high moisture or lipid was low in level.
  • 鈴木 健, 須山 三千三
    1983 年 49 巻 11 号 p. 1747-1753
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    The deistribution of free and conjugated amino acids in the trichloroacetic acid extracts of mature eggs of fish, such as chum salmon, trout, sweet smelt, pond smelt, goldfish, carp, saury, flying fish, puffer, and flathead flounder was examied. The composition of these amino acids fluctuated extensively, but a similarity of amino acid patterns was found among the eggs of fish which are taxonomically close to each other. The eggs spawned in sea water, except for puffer, had higher levels of free amino acids than the others. All eggs contained phosphopeptides, although the yield and amino acid composition of the phosphopeptides varied consierably from species to species; chum salmon and trout were rich in phosphopeptides compared with the other fish eggs. The amino acid pattern of ethanol precipittes obtained from the trichloroacetic acid extracts of many fish eggs resembled that of the phosphopeptides.
  • 渡辺 勝子, 前沢 博之, 中村 春夫, 鴻巣 章二
    1983 年 49 巻 11 号 p. 1755-1758
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
    Extractive nitrogen and free amino acids in the muscle of the cultured ascidian Halocynthia roretzi were analyzed on the specimens collected bimonthly from May 1980 to September 1981 at Okkirai Bay, Iwate Prefecture, and on the specimens collected in May and November 1980 at Onagawa Bay, Miyagi Prefecture, The ascidian muscles were rich in free amino acids, especially in taurine, proline, glutamic acid, glycine and alanine, but scanty in arginine, Most amino acids as well as extractive nitrogen showed a marked seasonal variation and lowest values were found in winter. Significant differences in some amino acids were observed between the specimens collected from the two localities in May.
    The ascidian seems to bear both characteristics of marine vertebrates and invertebrates with respect to the free amino acid composition of the muscle in line with its taxonomic position. It is suspected that the palatability of the ascidian muscle is closely related to the free amino acids, as their contents were considerably higher in the season when its flavor is better.
  • 渡辺 真利代, 大石 真之
    1983 年 49 巻 11 号 p. 1759
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 池田 弥生, 藤方 明
    1983 年 49 巻 11 号 p. 1761
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 丹羽 栄二, 中山 照雄, 浜田 巌
    1983 年 49 巻 11 号 p. 1763
    発行日: 1983/11/25
    公開日: 2008/02/29
    ジャーナル フリー
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