NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 49, Issue 2
Displaying 1-25 of 25 articles from this issue
  • Tasuku WATANABE
    1983 Volume 49 Issue 2 Pages 157-163
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Persistent infection in YNK cells derived from Yamame Oncorhynchus masou kidney with a non-pathogenic variant of infectious hematopoietic necrosis virus (IHNV) was established. The virus used to initiate persistent infection was isolated from rainbow trout Salmo gairdneri. Indirect fluorescent antibody studies showed that the persistently infected cells, YNK (SE-IHNVL), had similar antigenic properties to wild-type IHNV. YNK (SE-IHNVL) cells are presumably infected with a temperature-sensitive mutant of IHNV, because of a normal cell appearance and multiplication occur at 20°C but cytopathic effect (CPE) and destruction of the cells follows incubation at 15°C. YNK (SE-IHNVL) cells produce the virus (HS7912 IHNV) with weak cytopathogenicity on tissue culture cells constantly (103.8-104.8 50% tissue culture infective dose perml). This virus destroys cell monolayers at 15°C but not at 20°C. The infection of HS7912 IHNV was neutralized by anti-wild-type IHNV serum. HS7912 IHNV had so little pathogenicity against rainbow trout fry that only 1% of inoculated fish were killed, while the mortality of fish infected with IHNV was 40%. By superinfection study, HS7912 IHNV was found to infect tissue and to interfere with the infection of wild-type IHNV.
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  • Morihiko KUWA
    1983 Volume 49 Issue 2 Pages 165-175
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Samples of galvanized steel wire netting under water used in floating cage of chain link wire netting for culturing yellow tail Seriola quinqueradiata and Japanse striped knifejaw Oplegnathus fasciatus were observed for corrosion and tested to evaluate the cathodic protection by aluminum alloy anode method, and the following results were obtained.
    In galvanized steel wire netting, the galvanization service life was about 15 months in wire netting galvanized with 400g/m2, bout 10 months with 260g/m2 and about 9 months with 250g/m2. The wire gauge corrosion rate of these samples after consumption of galvanization was 0.4 to 0.6∅mm/yr.
    Although obvious differences were not observed in the species and volume of fouling organisms deposited on the wire netting due to differences in the corrosion or protection, the fouling organisms on the wire netting of cages culturing yellow tail were removed by predacious activities of Oplegnathus sp. crowding around the floating cages, and the state of removal of fouling organisms was similar to the condition observed on the wire netting surface of cages culturing Japanese striped knifejaw alone. In all those fish culturing cages in which aluminum alloy anodes were installed, the life of wire netting was notably extended thanks to the effect of cathodic protection.
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  • Mineo OKAMOTO
    1983 Volume 49 Issue 2 Pages 177-182
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Artificial seaweeds farm plant, experimental floating reef made of steel, had been anchored at the depth of fourty-three meters off Tajima in the Japan Sea. Mean component of the plant was a shelf (22×22m square) settled at ten meter depth layer and an observation tower rose at the center of the shelf toward the surface. Two years after the plant had been anchored, the activity of fishes around the plant was investigated by direct observation and by some automatic recording instruments. As results, some phenomena concerning the behavior of fishes were found as follows.
    (1) Around the plant, seventeen kinds of fishes were found and the dominants were Seriola aureovittata, Oplegnathus fasciatus, Sebastes inermis and Navodon modestus.
    (2) Distribution and activity of the fishes around the plant varied in the daytime and in the nighttime.
    (3) Fishes were classified into all day dependant type and daytime dependant type to the plant, but this classification seemed to vary according to the life-stage of each kind of fishes.
    (4) Most of the fishes, including young Navodon modestus, belonged to all day dependant type, and they distributed within twenty meters of the plant horizontally and vertically, all day.
    (5) Seriola aureovittata and adult Navodon modestus belonged to daytime dependant type, and they distributed thirty meters or more from the plant, in the daytime. In the nighttime, they were not observed swimming around the plant, and Navodon modestus was considered to rest on the bottom under the plant.
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  • Toshikuni NAKATANI, Tatsuaki MAEDA
    1983 Volume 49 Issue 2 Pages 183-187
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Form the end of 1979 to the early spring of 1980, the spawning ground of walleye pollock Theragra chalcogramma (PALLAS) was formed in the northeastern area from Shiraoi to Mukawa in contrast to that in recent years. The eggs spawned were transported toward the bay and many of them hatched befor they reached the inner area of the bay. The main food organisms of the larvae at the initial feeding stage were the eggs and nuaplii of copepods and most of them in the early spring were produced by the spawners of Pseudocalanus minutus in this study area. During the same period, the surface water of the Oyashio current was found only at the enerance of the bay, and the Tsugaru Warm Water occupied the inner area of the bay. For this reason, P. munutus were concentrated at the entrance of the bay and the distribution pattern of P. minutus coincided with that of walleye pollock larvae.
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  • Toshio OKAZAKI
    1983 Volume 49 Issue 2 Pages 189-196
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Chum salmon populations which distributed extensively from North America via the U. S. S. R. to Japan were investigated. Several regional populations consisting of adjacent river populations were identified from the gene frequency data. It is presumed that the accumulation of the genetic intermingling for generations lead to genetic similarity among river populations within one region. Chum salmon population is unique among the species of genus Oncorhynchus in having several regional populations. This characteristic presents a striking contrast to sockeye salmon and others in which the genetic isolation of river populations is more evident.
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  • Haruo SUGITA, Yoshiaki DEGUCHI
    1983 Volume 49 Issue 2 Pages 197-201
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Microflora in the contents of gastrointestine of soft-shelled turtle Trionyx sinensis was investigated using 7 different agar media. Citrobacter freundi, Edwardsiella tarda, Aeromonas hydrophil, Plesiomonas shigelloides, Streptococcus faecalis were prdominant, and total viable counts ranged from 107g-1 to 108g-1, in the stomach and small intestine. In the large intestine, obligate anaerobes, Bacteroidaceae and Clostridium, also predominated in addition to facultative anaerobes. Total viable cuntds ranged from 109g-1 to 1010g-1. In the diet, Bacillus, Flavobacterium, Micrococcus and Clostridium were predominant; the total viable count was 103g-1.
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  • Kaneshisa HASHIMOTO, Shugo WATABE
    1983 Volume 49 Issue 2 Pages 203-206
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Meat blocks (3×3×3cm) were prepared from a very fresh specimen of the bigeye tuan Thunnus obesus, and stored at -20°C, -40°C, -60°C, and -80°C, for one year. At one to three month intervals, the meat blocks were analyzed for the change in color, taking the metmyoglobin (metMb) to total Mb ratio as a parameter, and for the change in water holding capacity, taking the exprssible, cooking, and free drips as parameters.
    The MetMb to total Mb ratio increased from 3.9% at the start to 5-15% after one year storage at -40°C to -80°C. At -20°C, the metMb formation was clearly faster: 25% after 9 months and 56% after one year.
    The pH of the meat remained at a relatively constant value near 6.5 throughout the whole period, irrespective of the storage temperatures. The amounts of exprssible, cooking, and free drips from the meat showed some changes during the storage, which did not depend upon the storage temperatures.
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  • Reiji YOSHINAKA, Tohru SUZUKI, Mamoru SATO, Shizunori IKEDA
    1983 Volume 49 Issue 2 Pages 207-212
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Active form of trypsin was not found in the extract of the pancreas of the catfish Parasilurus asotus. Zymogens were activated autocatalytically to maximal level when the extract was incubated at pH 8.0 and 4°C for 4 days in the presence of calcium ions.
    The presence of two types of trypsin, anionic and cationic forms, in the autoactivated preparation of the extract was demonstrated by DEAE-cellulose column chromatography. Sephadex G-75 gel filtration and DEAE-cellulose column rechromatography were used for furhter purification. In the present study, an anionic trypsin was purfied to homogeneity.
    The molecular weight of catfish anionic trypsin was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to be 26, 000. The isoelectric point of this enzyme was determined to be 5.4 by isoelectric focusing. The amino acid composition of this enzyme was similar to that of anionic trypsins from some species of vertebrates, including fishes.
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  • Mutsuo HATANO, KOZO TAKAMA, Hirobumi KOJIMA, Koichi ZAMA
    1983 Volume 49 Issue 2 Pages 213-218
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The chemical composition of fall chum salmon Oncorhynchus keta muscle was determined and compared with that of feeding migrating migrating one (summer chum) in order to characterize the muscle of fall chum in respect to its utilization.
    Proximate compositions of the muscles of fall and summer chum were 16% and 20% protein, 1.6% and 8.5% lipid, and 80% and 72% moisture, respectively.
    The data calculated per whole fish muscle showed that the protein amout of fall chum was nearly comparable to that of summer chum. Lipid amount of fall chum was 130-140g less than that of summer chum.
    The amount of muscle (edible portion) of fall chum was 640-950g more than that of summer chum, the increment roughly matching that of the moisture in the fall muscle.
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  • Takashi Taneda, Takahiro WATANABE, Nobuo SEKI
    1983 Volume 49 Issue 2 Pages 219-228
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Calpain was purified from carp ordinary muscle by chromatography on DEAE-cellulose and Ultrogel ACA 34. The molecular weight was 80, 000 and the optimum pH 7.0. The half maximal activation of this calpain was obtained at 0.6-1.0mM Ca2+ and 4-5mM Sr2+, whereas Mg2+ did not activate this proteinase. Alkylating agents such as iodoacetic acid and N-ethylmaleimide inhibited the activity. From these rsults, carp calpain was classifined as calpain type II.
    Phase microscopic observations showed that carp myofibrils were shortened and fragmented when incubated with the calpain. SDS-polyacrylamide gel electrophoresis showed that the calpain released from carp myofibrils α-actinin and some components whose molecular weights were >200, 000, 200, 000 43, 000, 37, 000, 19, 000, and 14, 000. The bands corredponding to troponin T and troponin I became fainter than those in intact myofibrils during incubation of myofibrils with this calpain.
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  • Shigeru KIMURA, Hiroyuki TANAKA
    1983 Volume 49 Issue 2 Pages 229-232
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    The foot muscle collagen of top shell Turbo cornutus of the class Gastropoda was isolated by limited pepsin digestion of the muscular tissue, follwed by selective salt precipitation at 0.40M NaCl. Characterization of the isolated muscle collagen has suggested that it comprised three identical α1 chains and may correspond to Type I trimer of vertebrate collagens.
    We have recently reported that the arm muscle collagen of octopus belonging to the class Cephalopoda was compoed of two α1 chains and one α2 chain. Thus, the combined results demonstrated the interclass differences in chain composition of molluscan muscle collagens.
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  • Junichi MARUYAMA, Tamao NOGUCHI, Yoshio ONOUE, Yochi UEDA, Kanehisa HA ...
    1983 Volume 49 Issue 2 Pages 233-235
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    During 1980-1981, highly PSP-infested specimens of the scallop Patinopecten yessoensis were collected from Ofunato Bay. Iwate Prefecture. The scallop specimens were shucked, dissected, and examined on the anatomical distribution of the toxicity. Toxins were prpared from the fresh or frozen digestive glands and analyzed by TLC and electrophoresis.
    Toxification of the scallop in 1981 was much more intense than before. The toxicity reached 2, 100-3, 500MU/g in digestive gland, 210-600MU/g in rectum, 160-230MU/g in foot, 110-160MU/g in gonad, 75-110MU/g in mantle, 71-110MU/g in gill, and 16-43MU/g in addutuctor muscle. The toxicity of 1981 scallops was about 10 times higher in degestive gland and 2-4 times higher in other parts (except mantle) than that of 1980 scallops.
    In 1980 and 1981 scallops, gonyautoxin 1 was predominant; gonyautoxins 2-5, saxitoxin and neosaxitoxin were also found.
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  • Atsushi WAKAMEDA, Seiko NOZAWA, Ken-ichi ARAI
    1983 Volume 49 Issue 2 Pages 237-243
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The effects of neutral salts, such as KCl, NaCl and LiCl, in the thermal inactivations of myofibrillar Ca-ATPase of white croaker and carp have been studied. The myofibrils were kept at a fixed temperature in the range of 25-38°C with various concentrations (0.035-2.0M) of KCl or NaCl and the first order rate constants (kD) for inactivation of Ca-ATPase were estimated.
    Upon increasing the concentration (M) of neutral salt in the medium, the increase in kD proceeded in three first order steps: a very slow rate of increase from 0.035 to 0.30M, a rapid rate of increase from 0.30 to 1.1M (for white croaker) or to 1.5M (for carp), and remaining constant at a maximum above 1.1M (for white croaker) or above 1.5M (for carp). The linear rate plots of log kD vs. salt concentration (0.3-1.0M) showed that white croaker myofibrils were 2.5 times more susceptible to neutral salt than carp myofibrils. The following relative kD values for inactivation of Ca-ATPase by neutral salts were found: LiCl>NaCl>KCl.
    The effect of neutral salts on the kD for inactivation of its Ca-ATPase was identical for myosin B and for myofibrils from the same fish species.
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  • Eiji NIWA, Teruo NAKAYAMA, Iwao HAMADA
    1983 Volume 49 Issue 2 Pages 245-249
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The reason why the fish flesh gel (kamaboko) is hardened by prheating the fish flesh sol (surimi) at relatively low temperature (setting, suwari), was studied. The compression elastic modulus of the actomyosin (AM) gel, which was prepared from AM sol by heating at 80°C for 20min after preheating at 40°C for 60min, was higher than that of the gel prepard without preheating. The instantaneous shear moduli of AM gels, one prepared by heating AM sol at 40°C for 60min and the other prepared by further heating the first at 80°C for 20min, were almost the same at the respective heating temperatures, but they were higher than that of the gel prepared by heating the sol only at 80°C for 20min. The shear moduli of both gels prepared at 80°C were increased when the gels were cooled to 0°C, but the increase was remarkable for the gel from the preheated AM sol.
    Based on these results, the effect of setting on the network structure of protein in the fish flesh gel is discussed. With the progress of the setting, the proteins form a finely dispersed net-work structure without much liberation of water. This finely dispersed structure is kept even after heating the set gel to shape the fish flesh gel, and such a structure is conjectured to be essential for the elasticity of the fish flesh gel.
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  • Akiyoshi KAMATANI, Masanori TAKANO, Yoshimi MORITA
    1983 Volume 49 Issue 2 Pages 251-258
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    Six stations in Tokyo Bay were selected for studying the vertical and seasonal variations in the particulate matter chemistry. In summer the suspended particles above the thermocline were abundant in organic matter, and below the amount of the particles drastically diminished. In winter season the suspended particles enrichde with inorganic matter-mostly derived from the sea floor by the action of wind-driven mixing-distributed homogeneously throughout the water column.
    The avaiable silica depletion in the euphotic zone during summer within the Tokyo Bay ecosystem, which is highly eutrophied by phosphorus and nitogen compounds, was observed to act as a limiting factor for the diatom growth: diatoms will be replaced, at least partially, by other phytoplankton that do not consume silica because diatoms will not be able to compete with green and blue-green algea for avaiable pools of other nutrients when the silica supply is limited. Accordingly the ratios of biogenic silica to organic carbon in the suspended matter were changed markedly.
    The high constructions of reactive-silica and-phosphate in the bottom waters during summer suggested the fact that the nutrients liberated from the moiety of plankton accumulated on the bottom floor had their upward diffusion blocked by the presence of a discontinuity layer.
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  • Nobukazu SHIBATA
    1983 Volume 49 Issue 2 Pages 259-264
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    This work was carried out to examine the effects of fishing season, body length, and body section of Antarctic krill Euphausia superba on the content, component, and fatty acid composition of lipid which was extracted from krill immediatedy after catch.
    The lipid content increased from 1.11% of wen weight krill in the initial season of fishing (December) to 3.95% in the terminal season (February, next year). Phospholipid occupied 63.6% of total lipid in the intial season, but decreased to 44.5% in the ternimal one. Triglyceride increased from 5.5% to 30.1%. Per cent of free fatty acid in total lipid ranged from 0.4 to 4.2. Total cholesterol was constant for different fishing seasons. Fatty acid composition was different according to fishing season: C14:0, C16:1, and C17:1 increased, while C20:5 and C22:6 decreased, toward the terminal season of fishing.
    Almost no differences in lipid content, lipid composition, or fatty acid composition were recognized in krill of various body length, except gravid krill, caught in the same fishing net.
    The lipid conten of cephalothorax was about 2 times that of abdomen. In the lipid of cephalothorax, phospholipid was 44.0% and trglyceride was 36.0%; in that of abdomen, phospholipid was 52.0% and triglyceride 29.1%.
    Astaxanthin decreased in the total lipid toward the terminal season of fishing, but ranged in 3-4mg against 100g wet weight krill through the fishing season.
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  • Shugo WATABE, Yoshihiro OCHIAI, Satoshi KANOH, Kanehisa HASHIMOTO
    1983 Volume 49 Issue 2 Pages 265-268
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Proximate and Protein compositions of the ordinary muscle of requiem shark Triakis scyllia were examined. The proximate composition of the dorsal muscle was: moisture 76.0%, crude protein 24.9%, crude fat 2.7%, and crude ash 1.3%, The proein content corrected for urea (5.4mgN/g) and for non-protein nitrogenous compounds (11.2mgN/g) was 21.5% and 17.5%, respectively.
    The protein compositions of the dorsal and ventral muscle remained unchanged essentially through pre-, in-, and post-rigor stages: In the dorsal part, sarcoplasmic 22.1-25.9% (av. 24.5%), myofibrillar 60.1-66.8% (63.2%), alkail-soluble 4.1-5.5% (4.7%), and stroma fraction 6.5-8.9% (7.6%). In the ventral part, corresponding data were 20.4-26.1% (24.2%), 58.4-67.2% (63.5%), 2.9-9.2% (6.1%), and 3.8-8.2% (6.1%), respectively. The protein composition of each muscle did not show any significant changes after a 2-week frozen storage at -20°C.
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  • Shinichi NAGATA
    1983 Volume 49 Issue 2 Pages 269-271
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    Degradation rates of n-alkane substrate were obtained as functions of salinity (0-5%) and temperature (10°C-50°C) for marine petroleum-degrading bacteria, Corynebacterium sp. and Flavobacterium sp. For both strains, only a slight dependency on salinity was found in the range of 0-5% NaCl. Temperature dependency, however, was quite large, i.e., while the most appropriate temperature for n-hexadecane degradation was at ca. 30°C for both isolates, the activity was lost completely at 10°C and at 50°C.
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  • Michiko TANIGUCHI
    1983 Volume 49 Issue 2 Pages 273-281
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    In December, 1974, cultivated Monostroma were damaged in the estuary area of the Matsuda river, located in Sukumo City, Kochi Pref., in the western part of Shikoku Island of Japan. The plants of Monostroma were covered with a floating bacterial matrix, so that the production of Monostroma remarkably decreased. Based on the microscopic observation of damaged plants, water and mud analysis, and culture experiments of Monostroma plants using the floating bacterial matrix, it was found that the damage of the Monostroma was caused by the floating bacterial matrix. The floating bacterial matirix consisted of flocks of bacteria agglutinating with much sludge. The external appearance of damaged plants were as follows: (1) plants become fragile and ragged on the edge showing symptoms of a spot disease caused by the bacterial flock, (2) plants changed colour to deep green, (3) plants were covered with the slimy sludge. It was concluded that the floating bacterial matrixes originated from the area near the outlet of the night soil treatment plant which was located at the branch of the Matsuda river.
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  • Yuichi KOTAKI, Masahiro TAJIRI, Yasukatsu OSHIMA, Takeshi YASUMOTO
    1983 Volume 49 Issue 2 Pages 283-286
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Search for the origin of paralytic shellfish toxins occurring in coral reef crabs and gastropods resulted in a discovery of a calcareous red alga Jania sp. as the toxin progenitor. The alga was confirmed to contain gonyautoxins I, II and III, the first being the major component. The presence of this alga in the stomach of two toxic crabs Zosimus aeneus and Atergatis floridus and four gastropods Turbo marmorata, Turbo argyrostoma, Tectus pyramis and Tectus nilotica maxima evidenced that Jania sp. was the primary source of the toxins. Seasonal observation indicated that the toxin content in the alga reached the maximum in the spring.
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  • Hiroo YAMAMOTO, Shuichi SATOH, Toshio TAKEUCHI, Takeshi WATANABE
    1983 Volume 49 Issue 2 Pages 287-293
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    A long-term feeding experiment (60-62 weeks) was conducted to examine the effects of the deletion of manganese (Mn) or of the total deletion of trace elements from the mineral mixture in white fish meal diets on growth and chemical compositions of rainbow trout.
    The total deletion of trace elements from the diet results in reduced growth, lens cataract, and short body dwarfism as the most noticeable gross external signs. In addition the contents of Zn in vertebrae, testis, and ovary were also found to be very low, although a substantial amount of the Zn in vertebrae, testis, and ovary were also found to be very low, although a substantial amount of the Zn required by the fish was contained in the diet derived from the white fish meal used. These results suggest that the availability ot rainbow trout of Zn contained in white fish meal is very low.
    The fish fed on the Mn-deficient diet developed lens cataracts and short body dwarfism, but the growth was not much affected by Mn deficiency. The contents of Mn and Zn vertebrae, testis, and ovary of this group were significantly lower than those in the fish receiving the control diet. On the other hand, feeding of the fish with a white fish meal diet without a supplement of riboflavin caused a deficiency symptom.
    These results have shown that even though white fish meal containing various kinds of minerals and riboflavin is used as a protein source, a supplement of dietary Mn and Zn or of riboflavin is essential for rainbow trout.
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  • Hiroyuki YAMAMOTO, Yoshio EZURA, Takahisa KIMURA
    1983 Volume 49 Issue 2 Pages 295-300
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
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    Changes of bacterial flora in natural seawater and in seawater inculated with Vibro parahaemolyticus during storage were examined. In both samples, the dominant group of bacteria changed rapidly, although the total number of viable bacteria maintained a constant level after the initial increase. The organic substrate decomposer groups, such as Alteromonas and Vibio, which were initially the predominant groups in the natural seawater, were rapidly replaced by the organic substrate nondecomposer groups, such as Pseudomonas, Moraxella and Acinetobacter.
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  • Shoji KONOSU, Katsumi YAMAGUCHI, Shinya FUKE, Takaaki SHIRAI
    1983 Volume 49 Issue 2 Pages 301-304
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In order to examine extractive components of salmonids not only from the biochemical aspect but also from the food chemical point of view, we analyzed the amino acids and related compounds in the 80% ethanol extracts of the dorsal, ventral, and caudal muscles of both sexes of chum salmon as the first step of a series of investigation. The most remarkable feature was that a large anount of dipeptide anserine was present with fairly lower levels of histidine, taurine, alanine, and glycine in each extract. These five constituents comprised about 90% of the total free amino acids and related compounds. The composition of the constituents was almost the same, irrespective of part and sex. The heating of the muscles before extraction and the acid hydrolysis of the extracts also gave little change in the composition. However, the heating increased the amount of extractive nitrogen without exception. This result suggests the formation of low-molecular-weight nitro-genous compounds other than the amino acids and related compounds.
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  • Yoshiaki HARADA
    1983 Volume 49 Issue 2 Pages 305
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • Tasuku WATANABE, Kunio YAMAMORI
    1983 Volume 49 Issue 2 Pages 307
    Published: February 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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