巻号一覧
49 巻, 3 号
選択された号の論文の25件中1～25を表示しています
- |<
- <
- 1
- >
- >|
-
糸洌 長敬, 三木 誠1983 年 49 巻 3 号 p. 339-346
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーField observations and analysis of wave-induced circulation in Masawa, Hachijyo Island were made and the generating meachanism of the circulatin was investigated by introducing the radiation stress conept. Our results show that circulation cells exist constantly, and the differences between the gradient of the radiation stress and mean water level gradient, which are the driving forces to induce wave current, are produced by the differences of local wave height assoiated with the rafrac-tion of wave due to the undulation of seabottom topography. The survey results show that when the offshore current flwo is great, the circulation cell on the downstream side is strengthened, while that on the upstream side is weakened. More over, in the clodkwise and anticlockwise cell forming area, the marine algae community and the area densely inhabited by ormer Sulculus diversicolor diversicolor correspond well with one another.抄録全体を表示PDF形式でダウンロード (570K) -
坂井 勝信1983 年 49 巻 3 号 p. 347-351
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーSera of fish, including a wide of species from elasmobranchs to teleosts, possess two kinds of hemolytic activities against heterlolgous red blood cells: one is a spontaneous (non-specific antibody-independent) hemolytic activity (SH50) against unsensitized red blood cells; and the other is a hemolytic complement (specific antibody-dependent) activity (CH50) against red blood cells sensitized with their homologous antibodies. SH50 activity of rainbow trout serum completely disappeared by heating at 44°C for 20min, a condition which is specific to the heat inactivation of this complment. Pronase, LPS (isolated from Salmonella enteritidis) and zymosan, which are wellused chemical agents of non-specific activators for an alternative pathway of a mammalian complement system, drastically reduced SH50 and CH50 activities when the serum was reacted at 30°C for 2 hours with these chemicals. An effect similar to the results in vitro could be produced by the intraperitoneal administration of pronase and LPS into rainbow trout. However, zymosan did not have the ability to lower the SH50 and CH50 activities, because of water-insoluble undiffusive particles. These data show that SH50 activity is based on alternative pathway activation and, therefore, that an alternative pathway is evidently present the complement sytem of rain bow trout serum.抄録全体を表示PDF形式でダウンロード (372K) -
西村 昭史, 関 政夫1983 年 49 巻 3 号 p. 353-358
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーEffects of liming for the improvement of mariculture grounds were studied in a model system.
Lime (CaO) increased pH of the sea water and consequently sulfide and ammonium ion were eluted from the bottom mud. Lime transmuted gradually into magnesium hydroxide as a result of the exchange fo Ca2+ for Mg2+. Lime and magnesium hydroxide efficiently inhibited the growth of sulfate-reducing bacteria for a long term, and accordingly the formation of hydrogen sulfide was reduced. Amount of lime required to prevent the formation of hydrogen sulfide in mariculture grounds was estimated to be 100-200g/m2.
These results suggest that lime is an effective material for the improvement of mariculture grounds.抄録全体を表示PDF形式でダウンロード (443K) -
乾 靖夫, 松里 寿彦1983 年 49 巻 3 号 p. 359-362
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーA new preparation for in vitro study of muscle metabolism of the eel was described. A thin muscle layer was easily isolated from the operculum of the eel, with the isolated tissue exhibiting a thickness fo 344±14μm. Freshly prepared muscle appeared hitologically intact. After short term (2hr) incubation, the muscle showed focal liquefaction degeneration and the degenerated area increased with the duration of incubation. However, a subtantial portion of the muscle remaied largly intace even after long term (15hr) incubation. From the initiation of incubation, inulin space increased for 30min and reached a level of 35-40, which was maintained approximately constant over the subsequent in cubation period of 15hr.
These results indicate that the isolated opercular muscle of the eel is comparable to mam-malian cut diaphragm and that the eel tissue may be useful for in vitro studies of muscle metabolism of fish.抄録全体を表示PDF形式でダウンロード (469K) -
谷口 道子1983 年 49 巻 3 号 p. 363-366
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーTo clarify the effects of food quality on the appearance of yellowtail streptococcicosis, the tolerance of the fish fed with various quality of foods during the experimental period was examined bsed on the artificial peroral inoculation method. The vitamin mixture was surely effectve to decrease mortality from the diseases during hot season. When garlic extract was fed besides the vitamin mixture, seriously infected individuals were small in number. The incidence of the disease was very low in the experimental group fed with frozen fish in comparison with the experimental group fed with thawed fish. The experimental groups fed with washed fish or the thawed fish mixed with ethoxyquin also showed low incidnce of the diseases compared with the group fed with thawed fish. Conclusively, feeding with frozen with frozen fish before thawing was the best among the other groups fed with thawed fish.抄録全体を表示PDF形式でダウンロード (315K) -
川崎 健, 橋本 博明, 本多 仁, 大竹 敦1983 年 49 巻 3 号 p. 367-377
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーGrowth rate of snailfish is very high, eggs produced in late December-Janmary becoming 400mm in body length for male and 320mm for female in the same period of the next year, and their life span is one year. Food habits shift from “number selection” depending manily on crustaceans to “size selection” relying in particular on sandeel in October when growth is the fastest. Spawning seems to be carried out in several batches of eggs 1.4-1.5mm across and the fertility is estimated to be a hundred thousand and tens of thousands. Snailfish supposedly raises the probability for eggs to survive through caring for them by their fathers. Short life of snailfish along with parental care for eggs brings about a rise of the intrinsic rate of natural increase rm, resulting in an elaborate regulation of population numbers.抄録全体を表示PDF形式でダウンロード (620K) -
梨本 勝昭, 宮沢 晴彦, 平石 智徳1983 年 49 巻 3 号 p. 379-385
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーThe teeth on the dredge for catching Jpanese surf clams Pseudocardium sybillae are known to be highly selective. In this paper, the various factors affecting the selective action of the teecth of the dredge are analyzed, and the probability of the Japanese surf clams not sifting through the gaps between teeth was calculated for the various shell lengths. Considering that this probability is in proportion to the relative catching effciency, selectivity curves for the Japanese surf clam dredges were estimated for various gaps between teeth. The ratio of the shell length of the Japanese surf clam ot the distance between teeth indicating the relative catching efficienciesof 0%, 50% and 100% respectively, were calculated by using the selective curves. These ratios showed a slight tendency to decrease as the distance between the teeth increased, but for a distance of 4.0cm, these values were 1.22, 1.34 and 1.83 respectively.抄録全体を表示PDF形式でダウンロード (605K) -
Roland BILLARD, 隆島 史夫1983 年 49 巻 3 号 p. 387-392
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーPerliminary investigations on the loss of spermatozoa in the sperm duct of rainbow trout during the post-spawning period showed that the spermatozoa degenerated in situ in the duct lumen or were phagocytized there by free phagocytes and by the epithelial cells. Drasstic alterations occurring in the epithelium between March and April were characterized by excretory activity in March and intensive phagocytic activity in April when the process of sperm release had nearly stopped. A possible relation between the stuctural and functional changes in the sperm duct and endocrine balance were discussed.抄録全体を表示PDF形式でダウンロード (883K) -
池田 弥生, 太田 裕達, 尾崎 久雄1983 年 49 巻 3 号 p. 393-397
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーAs ?? a ?? fundamental step to settle the haemochemical assessment of health or disease in fish, the variation of plasma constituent levels on the storage time or temperature was investigated in carp Cyprinus carpio, of 500-1, 000g in weight. The blood samples were taken by heart puncture using sodium heparin as anticoagulant. After 10min centrifugation at 3, 000rpm immediately after sampling, plasma samples were extracted and divided into 11 or 12 small tubes and stored at 5°C or -20°C. One sample was pool plasma from 2 or 3 fishes. 6 Constituent levels and 7 enzyme activities were measured in plasma samples stored at 5°C or -20°C for 0, 1, 3, 7, 15, and 30 days. And further, 7 enzyne activities were measured in plasma samples stored at -20°C for 3 hours immediately after sampling.
Activities fo GPT, CPK and LDH decreased quickly with the lapse fo stored time. GPT activity decreased to about 90% after 24 hours at 5°C, and to about 70% after only 3 hours at -20°C. CPK activity decreased to about 85% after 24 hours at both 5°C and -20°C. Therefore, it is desirable that these levels are mesured immediately after blood sampling. GOT and Al-P activities were stable for 7 days at 5°C, and for 30 days to -20°C. Acid P activity and levels of glucose and uric acid were stable for 15 days at 5°C. LAP activity and levels fo urea-N, total protein, ablumin and inorganic P were stable for 30 days at both 5°C and -20°C.抄録全体を表示PDF形式でダウンロード (391K) -
今田 克, 斉藤 祐一1983 年 49 巻 3 号 p. 399-407
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーIn tank culture of laver Porphyra, powdered materials were experimentally applied to substratum for germination of conchospore, instead of netting twine. As the first screening objects, 17 materials were picked, adequate sizes of these materials were determined to make the most uniformly suspended state in a culture vessel. Laver germlings grown on the powdered material were cultured under definite aritficial conditions using 2 liter vessel. Screeing of powdered materials as substratum was performed by comparison of the values of diurnal growth rate calculated from final thallus area of germlings. Under the most uniformly suspended state of powdered material, the laver genmlings showed the same growth rate as the cuture using netting twine and the hightest growth ratc was obtained in the case using powdered material substrata having rough surface and high density. As the rresult, in respect of germination of conchospores and growth of thallus, powder made from pearl-oyster shells were suitable for substratum; in respect of suppression of contamination by other algae, bone bladk and charcoal powder were suitable. Finally, pearl-oyster shell dyed black showed the best result, gremlings grown on them grew into young plants 5-10mm in length and 2-3mm in width for 30 days.抄録全体を表示PDF形式でダウンロード (1120K) -
C. P. ANAND, 柿本 大壱, 坂田 泰造1983 年 49 巻 3 号 p. 409-414
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーThree strains, namely the K+ and K- (Kanagawa positive and negative) strains of. V. parahae-molyticus and a strain of V. alginolyticus were used to study the varation of flagellar morphology in different cultural conditions. All three strains showed M-(monotrichous) flagella in liquid media in different studies. But on solid media, the flagellar morphology varied depending on the type of meadia, levels of pH, NaCl concentrations, growth temperatures and other cultural conditions. Examination of flagella during the growth period of the statins revealed that V. alginolyticus and K+ strain of V. parahaemolyticus fist showed PM-flagella and then changed to P-(peritrichous) flagella, whereas K-strain showed P-flagella throughtout the growth period. The strains developed M-flagella when they were grown on TS3% NaCl medium containing gelatin (7%) and agar (0.75%) as solidigying agents. However, the same strains showed either mixed type or P-flagella when they were grown on ZoBell 2216E medium made with either different concentrations of agar of carrageenan. It was observed that the relation between swarming and P-flagellation depended on cultural conditions. The results obtained in the present study indicate the need for standardization of cutural conditions when estabishing flagellar morphology.抄録全体を表示PDF形式でダウンロード (1023K) -
大谷 修, 引地 聰, 西田 清義, 関井 利之, 新井 健一1983 年 49 巻 3 号 p. 415-424
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーWhen myofibrils were prepared from various muscles of shell-fishes and of fishes by repeated washing with low ionic strength medium, more actin was released from the shell-fish myofibrils rather than from the fish myofibrils. This conclusion was based on the following results. (1) The inactivation mode of Ca-ATPase activity changed from the typical fist-order process to biphasic first-ored behavior: the rate for inactvation increased as the washing repeated. (2) The typical fisrt-ored inactivation of Ca-ATPase was reversibly restored by the addition of rabbit skeletal F-actin to the washed myofibrils. (3) A component coresponding to acin on the SDS-gel electro-phoresis was specifically decreased in the washed myofibrils, while it was increased in the supernatant and precipitated upon complexing the myosin.
The same results show that the loss of actin occurred more readily in the fish dorsal musule myosin B than in the rabbit skeletal muscle myosin B.抄録全体を表示PDF形式でダウンロード (1008K) -
佐藤 秀一, 山本 裕夫, 竹内 俊郎, 渡辺 武1983 年 49 巻 3 号 p. 425-429
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーRainbow trout were fed on fish meal diets without a supplement of one trace element (Zn, Mn, Cu, Co) or magnesium (Mg) for 93 weeks in order to investigate the availability to the fish of these minerals contained in white fish meal.
Feeding a diet without a supplement of Zn caused cataracts (100%) together with short body dwarfism (80%) and depressed growth. These results were found to be very similar to those obtained in the fish receiving the diet without a supplement of any trace elements. The deletion of Mn or Mg from the mineral mixture in fish meal diets resulted in cataracts; the abnormality was 80% and 29% respectively at the end of the feeding experiment. On the other hand, the growth was not much affected by the deletion of Mn or Co from the diet.
The deletion of Zn also resulted in reduced levels of Mn and Cu, and that of Mn, Cu or Co resulted in a low level of Zn in vertebrae. suggesting an interaction between Zn and Mn, or Cu or Co in the fish.
Thus, each supplement of dietary Zn, Mn, Cu or Mg is found to be essential for rainbow trout, even though white fish meals contain various kinds of minerals.抄録全体を表示PDF形式でダウンロード (509K) -
佐藤 秀一, 山本 裕夫, 竹内 俊郎, 渡辺 武1983 年 49 巻 3 号 p. 431-435
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーA long-term feeding experiment (83 weeks) was conducted to examine the effect of the deletion of each trace element (Zn, Mn, Cu, Co) or magnesium (Mg) from the mineral mixture in white fish meal diets on growth and mineral composition of common carp.
The deletion of Mn from the fish meal diet caused cataracts (70%) together with short body dwarfism (90%) and depressed growth. These results were found to be very similar to those obtained in the fish receiving the diet without a supplement of any trace elements. The deletion of the other elements from the diets also resulted in cataracts (40-70%) and reduced growth. On the other hand, short dody dwarfism was not induced by the deletion of Zn from the fish meal diet, quite different from the case of rainbow trout. The deletion of Mn resulted in reduced levels of crude ash, Ca, Mg, P, and Mn in vertebrae. Furthermore, the content of Mn in vertebrae of carp was found to be significantly lower than that of rainbow trout.
These results have shown that the supplement of dietary Zn, Mn, Cu and Mg to fish meal diets are each essential for common carp.抄録全体を表示PDF形式でダウンロード (766K) -
小玉 修嗣, 今野 久仁彦1983 年 49 巻 3 号 p. 437-442
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーMyosin was separated from squid brachial muscle by essentially the same method as has been used for squid mantle myosin. Brachial myosin thus partially purified was contaminated with large amounts of paramyosin, which was, in turn, eliminated by the method of KIMURA et al. SDS-polyacrylamide slab gel electrophoresis showed that molecular weights of a heavy chain and two light chains of this myosin are identical to those of corresponding chains of squid mantle myosin.
Ca-, Mg- and EDTA-ATPase activities of the brachial myosin were similar to those of the mantle myosin in terms of the dependency on pH, KCl concentration, temperature, and actin activation of Mg-ATPase.
The Mg-ATPase activity was as Ca2+-sensitive as that of the mantle myosin, half the maximal activation being obtained at 3 μM Ca. The Ca-sensitivity was lost when 1.5M urea, 0.45M guanidine-HCl, or 3.6% n-butyl alcohol was present in the reaction mixture. Trinitrophenylation deprived the brachial myosin of Ca-senstitvity.
These results indicated the identity of both squid myosins in enzymatic properties including Ca-regulatory aspects.抄録全体を表示PDF形式でダウンロード (713K) -
佐藤 守, 近藤 隆雄, 吉中 禮二, 池田 静徳1983 年 49 巻 3 号 p. 443-446
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーThe effect of rearing water temperature on the skeletal deformity in ascorbic acid-deficient rainbow trout, weighing 0.17g on average, was investigated.
Three groups of fish were fed a diet at one of three ascorbic acid levels, i.e., 0, 2, and 200mg of L-ascorbic acid per 100g of diet, and were maintained either at 16°C or at 20°C for 20 weeks. Two other groups were given a diet at one of two ascorbic acid levels, i.e., 0 and 200mg of L-ascorbic acid per 100g of diet, and maintained at 16°C for the first 10 weeks and at 20°C for the last 10 weeks.
Lower growth was observed in the groups maintained at 20°C irrespective of dietary ascorbic acid levels. The skeletal deformity was observed only in the ascorbic acid-deficient groups. The rate of development of skeletal deformity increased markedly in the group which was maintained initially at 16°C with subsequent rise of water temperature to 20°C. These results indicate that underhydroxylated collagen in the tissues of ascorbic acid-deficient fish is denatured and digested quickly at higher water temperature, resulting in the low collagen content of the connective tissues, and finally developing a fragile bone structure.抄録全体を表示PDF形式でダウンロード (313K) -
城 久1983 年 49 巻 3 号 p. 447-454
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーThis report discusses fractionation of phosphorus in sediment cores of Osaka Bay and the releasable fraction from sediment to sea water in anaerobic condition.
Inorganic phosphate in sediment mud was fractionated into four types: soluble-P, Al-P, Fe-P, and Ca-P by the method of CHANG et al..
Concentrations of Al-P and Fe-P indicate the highest value in 0-5cm layer except for Al-P in Stn. 4 which is located at the most inner part of the bay. Ca-P contents show relatively high value for each sediment core, ranging from 110 to 190 μg per g dry mud, but area and vertical variances were not so clear.
As Al-P plus Fe-P contents in the sediment increase exponentially with ignition loss, most phosphates which are associated with pollutant substances in the sediment mud are in these two fractions.
Three fractions of phosphate: soluble, aluminum and iron phosphate may be released from the sediment in anaerobic condition. It was proved that contents of phosphate released into sea water increased proportionally to those three fraction contents in sediment mud.抄録全体を表示PDF形式でダウンロード (518K) -
村上 正忠, 藤沢 浩明1983 年 49 巻 3 号 p. 455-462
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーOn the coast of Hiro Bay in Seto Inland Sea, there is a kraft pulp mill whose effluent flows into the bay. Many workers have reported about organic pollutants and their influence on fishery in this area. Although microbiological work is indispensable for clarifying the mechanism of the decomposition of organic pollutants, little has been known about it. Therefore, a series of studies has been undertaken to clarify the microbial decomposition of hemicellulose in coastal waters polluted with pulp mill effluent. The distribution of heterotrophic bacteria and hemicellulose-decomposing bacteria, and the environmental conditions of seawater in the above-mentioned area were investigated five times from 1979 to 1981. The results obtained can be summarized as follows: The population densities of heterotrophic bacteria, glucomannan-decomposing bacteria, xylandecomposing bacteria and agar-decomposing bacteria per ml of seawater, were 102 to 107, 101 to 105, 101 to 106 and 101 to 105, respectively. The significance test of common coefficient regression analysis in the 1m layer of seawater showed that the population density of bacteria and the value of COD decreased with the distance from pulp mill. The ratio of the population density of xylan-decomposing bacteria to the population density of heterotrophic bacteria in the 1m layer of seawater was 10 to 1, 000 times as high as that in the control area (Yoshimi Bay), and similar to that in other pulp mill polluted ares (Iwakuni waters). Therefore, it can be said that this ratio may be one of the suitable indices for the pollution from plup mill.抄録全体を表示PDF形式でダウンロード (557K) -
吉中 禮二, 佐藤 守, 土谷 望, 池田 静徳1983 年 49 巻 3 号 p. 463-469
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーThe effective utilization of endogenous visceral enzymes was examined for the production of fish sauce from the sardine Sardinops melanosticta. The sardine visceral extract contained several proteases, which are possibly of pancreatic origin. The proteolytic activity in the extract was stabilized by calcium ions. The maximal rate of hydrolysis of sardine flesh by the visceral enzymes was obtained at pH 8.0 and 50°C in the presence of calcium ions. The addition of sodium chloride and the heat-treatment of the flesh reduced the rate of hydrolysis of the flesh by the visceral enzymes.
A new method of the production of fish sauce from the sardines was established. Sardine flesh homogenate was inculbated with the visceral extract for 5h under optimal conditions as described above. After incubation, the mixture was clarified by centrifugation. In the present study, 25% sodium chloride was added to the supernatant. However, this salt concentration can be changed at will to get the desirable product. The sardine fish sauce thus obtained and two commercial fish sauces, “Shottsuru”, were analyzed for chemical composition, including amino acid composition, and were evaluated for the sensory characteristics. The sardine fish sauce was comparable in quality with the two commercial products.抄録全体を表示PDF形式でダウンロード (480K) -
渡辺 武, 田宮 知則, 岡 彬, 平田 満, 北島 力, 藤田 矢郎1983 年 49 巻 3 号 p. 471-479
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーExperiments were conducted in order to improve the dietary value of live foods, such as rotifers, Artemia nauplii and Moina, by allowing them to feed on ω3 HUFA and fat-soluble vitamins by the dirct method. In this method lipids containing ω3 HUFA and fat-soluble vitamins were given, together with baker's yeast, directly to living feeds in the form of a homogenate of lipid with a small amount of raw egg yolk and water, resulting in a stable emulsion.
In experiments conducted on a small scale rotifers were found to take up lipids very easily from the emulsion. The concentration of ω3 HUFA in rotifers was proportional to the content of ω3 HUFA in the emulsion, and reached a maximum after between 6 and 12h of feeding. The same kinds of results were obtained with rotifers in the experiment conducted on a large scale comparable to that required for the practical seed production of fish. Results similar to those in rotifers were obtained in both Artemia nauplii and Moina, although the concentration of ω3 HUFA in the nauplii fatty acids was far less than that in rotifers and Moina. Supplemental vitamins A and E, added to lipid. were both found to be incorporated by rotifers together with the ω3 HUFA. On the other hand, there was no marked difference in the concentration of ω3 HUFA in living feeds due to the difference of emulsifier among soybean lecithin, casein-Na, or raw egg yolk.
Thus, the direct method has been shown to be effective for improving the dietary value of live foods in the same manner as the indirect method.抄録全体を表示PDF形式でダウンロード (574K) -
牧之 段保夫, 豊原 治彦, 池田 静徳1983 年 49 巻 3 号 p. 481-484
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーTo asist the search of proteinases which participate in fish muscle autolysis, we investigated the effect of protease inhibitors on the autolysis. The autolysis was done in the presence of 3 mM NaN3 at 37°C. The degree was determined by the colorimetric quantitative assay of 5% trichloroacetic acid soluble fraction after the autolysis and by the qualitative method of comparing electrophoretic patterns. In this study we used muscle homogenate instead of muscle block from the necessity of mixing inhibitor. Though the autolysis of homogenate proceeded faster than that of block, it was considered to reflect roughly the autolysis of muscle block. The effects of inhibitor were similar in the quantitative and the qualitative methods. Soybean trypsin inhibitor, PCMB, and pepstatin had no effect, but EDTA tended to inhibit. This result suggested the participation of metallo proteinase in the autolysis. But CaCl2 had no effect. Moreover, the effect of leupeptin suggested the participation of serine or systeine proteinase. Though iodoacetic acid showed a remarkable effect, it is still not clear whether the effect depends on proteinase.抄録全体を表示PDF形式でダウンロード (1271K) -
小山 絹江, 野口 玉雄, 宇津 淳, 橋本 周久1983 年 49 巻 3 号 p. 485-489
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーResistibility of a toxic crab Atergatis floridus possessing paralytic shellfish poison (PSP) and of five nontoxic crabs, Pilumnopeus indica, Pachgrapsus crassipes, Leptodius exaratus, Eriphia laevimona and Gaillardiellus orientalis, against PSP and tetrodotoxin (TTX) was examined. The results showed that A. floridus is highly PSP resistible, the minimum lethal dose (MLD) being 5, 000-10, 000 MU/20 g body weight. This crab showed essentially the same resistibility against scallop PSP mainly consisting of gonyautoxins and gaginst crab PSP Mainly consisting of saxitoxins. A. floridus exhibited also a fairly high TTX resistibility, with an MLD of 1, 000-2, 000 MU/20g.
On the other hand, the nontoxic crabs had no resistance against PSP and TTX at all; most of their MLDs were assayed to be only several MU/20g.抄録全体を表示PDF形式でダウンロード (326K) -
渡部 終五, 伊藤 康男, 橋本 周久1983 年 49 巻 3 号 p. 491-497
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーG-Actin was extracted from the acetone powder of the dark muscle of mackerel Pneumatophorys japonicus japonicus, and purified by a method which essentially consisted of polymerization in 0.1 M KCl containing 0.1 mM MgCl2, treatment with 0.6 M KCl, and Sephadex G-200gel filtration.
Sedimentation constant (s020, W) of the dark muscle actin was 2.9S. The molecular weight was determined to be 42, 000by SDS-gel electrophoresis, The intrinsic viscosity [η] was 5.3 dl/g in F-form. It featured higher glutamic acid, aspartic acid and alanine contents, and lower histidine and tryptophan contents. One mole of 3-methylhistidine per mole actin was detected.
The above physicochemical properties were essentially the same as those of actins from various sources, including mackerel ordinary muscle.抄録全体を表示PDF形式でダウンロード (1974K) -
野口 玉雄, 丸山 純一, 尾上 義夫, 橋本 周久, 池田 武彦1983 年 49 巻 3 号 p. 499
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (98K) -
太田 博巳, 高野 和則, 伊沢 敏穂, 山内 晧平1983 年 49 巻 3 号 p. 501
発行日: 1983/03/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (1142K)
- |<
- <
- 1
- >
- >|