All issues
Volume 49, Issue 4
Displaying 1-25 of 25 articles from this issue
- |<
- <
- 1
- >
- >|
-
Kazutsugu HIRAYAMA, Hiromichi FUNAMOTO1983 Volume 49 Issue 4 Pages 505-510
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe culture of the sterilized rotifer Brachionus plicatilis with washed cells of a baker's yeast revealed that the yeast had no nutritive effect on the pupulation growth of the rotifer.
Nutritive quality of experimental suspensions was evaluated by two kinds of culture, one of which was individual culture in which the rotifers were cultured separately in test tubes and observed daily to obtain the survival rate and fecundity while food suspension was renewed daily. From daily counts, intrinsic rate of population increase and net reproduction rate were calculated as the indices of nutritive quality of the suspension. The other was batch culture in which teh rotifers were maintained in the food suspension without any daily treatment. After 6 or 7 days culture, the increase in individual number was counted.
Out of the nutrients tested on the supplementary effect, vitamin B12 was most effective. Pollack liver oil (feed oil) and cystine were also effective in strengthening the nutritive quality of the yeast cell suspension. None of the oil spluble vitamins could substitute for the supplementary role of the feed oil.View full abstractDownload PDF (524K) -
Kenji KAWAI, Riichi KUSUDA1983 Volume 49 Issue 4 Pages 511-514
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPrevious papers on oral bacterin against vibriosis in cultured ayu plecoglossus altivelis showed that fish were effectively immunized with heated bacterin and that the agglutinin secreted in the body surface mucus of the immunized fish prevented pathogenic bacterium Vibrio anguillarum form adhering to the skin of ayu. These findings suggested that a heat stable antigen on the surface of the bacterium would play an important role in the protection against vibriosis.
In this study it was investigated whether that lipopolysaccharide, a heat stable antigen in the cell wall, of V. anguillarium would be an effective immunogen or not. The lipopolysaccharide fraction extracted by WESTPHAL's method from the formalin killed bacterin was used as the extracted vaccine. Efficacy of the extracted vaccine was estimated by comparing mortality in the fish immunized with the extracted vaccine to that in the fish immunized with the formalin killed bacterin after challenge. Challenge test was executed by exposing fish to the bacteria discharged from the fish injected with the virulent strain. Administration of the extracted vaccine at a level of 25mg/fish kg/day for 9 days resulted in sufficient protection similar to that of the formalin killed bacterin at a level of 0.1g wet cells/fish kg/day for 9 days. Chemical analysis showed that the extracted vaccine contained a large percentage of lipopolysaccharide and nucleic acid but only a small percentage of protein and liped. From these results it is presumed that lipopolysaccharide is the protective antigen of the bacterin.View full abstractDownload PDF (319K) -
Akihiko KUWAHARA, Shigeki SUZUKI1983 Volume 49 Issue 4 Pages 515-520
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFish larvae samplings were made in the western Wakasa Bay, the Japan Sea during May and June, 1981. In view of the vertical distribution and feeding, differences among three species of Scorpaenidae larvae, Sebasticus marmoratus (CUVIER), Sebastes oblongus GÜNTHER and Sebastes pachycephalus pachycephalus TEMMINCK et SCHLEGEL are to be expected.
The larvae of three species distributed abundantly in the shallow waters, especially in the water between 0m and 25m depths.
Plankton species belonging to Crustaceae was utilized as main food for all three species, of which S. marmoratus and S. pachycephalus pachycephalus began to feed on copepod nauplii and invertebrate eggs at yoek-sac stage. Copepod nauplii was indispensable food for three species larvae of less than 6.0mm BL. The main food of S. pachycephalus pachycephalus of more than 7.0mm BL were Evadne nordmanni, Acartia clausi, Paracalanus parvus, Oithona spp. and Calanus helgolandicus, and the food composition in the gut was very similar to plankton composition observed in the sea. Main food items were P. parvus, E. nordmanni and copepodites in S. marmoratus larvae of more than 6.0mm BL, and E. nordmanni, copepodites and copepod nauplii in S. oblongus larvae of more than 6.0mm BL.
The relationships between larvae size of three species and their food size, suggests that the larvae of S. marmorautus and S. pachycephalus pachycephalus discriminate prey size as growth proceeded.View full abstractDownload PDF (434K) -
Katsuhiko HARADA1983 Volume 49 Issue 4 Pages 521-526
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe attraction index a, which is one of the coefficients of a logistic curve y=g/{1+exp[-r(j-a)]} in the remaining time-course of test compartment, was examined for 42 specimens comprising of aquatic and terrestrial organisms used as test materials. Two species of worms of the oligochaets, one species of freshwater mussel of the bivalves, two species of sawtooth caridina and lake prawn of the crustaceans, and one species of the cow in terrestrial animals were effective in attracting oriental weatherfish. The oligochaets were most effective.
The behavioral observations lead to the following suggestion: The attraction by the three representative organisms, the worm, the freshwater mussel and the sawtooth caridina depended closely on the three chemical constituents, non-proteins N, amino acids and volatile bases N. This validity was furthermore confirmed by teh significance test of correlation coefficient between the attraction index a and the contents of these three constituents. In this context, three constituents of which at least two, amino acids and volatile bases N, mainly contained in the fraction of nonproteins N, appear to be the most probable components in feeding attractants for oriental weather fish.View full abstractDownload PDF (433K) -
Takashi MINAMI1983 Volume 49 Issue 4 Pages 527-532
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe early life history of a flouder Tanakius kitaharai was studied in the western Wakasa Bay, the Japan Sea.
General morphology and characteristics of the eggs, larvae and juveniles was described. The distinct feature of this fish in early larval stages was distributional patterns of melanophores on the body surface, forming three transverse bands.
Judging from the occurrence of eggs, spawning may occur during the relatively short period in February in the western Wakasa Bay.
Eggs and pelagic larvae were caught mainly around the mouth of Wakasa Bay and offshore region, early juveniles were caught mainly in the bottom between 110 and 130m in depth, and these areas may be their nursery ground.
Analyses of the gut contents showed that the food items are copeopd nauplii, diatoms, invertebrate eggs, copepods in pelagic larvae, copepods in early juveniles, and polychaetes in late juveniles.View full abstractDownload PDF (427K) -
Takashi MINAMI1983 Volume 49 Issue 4 Pages 533-539
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe early life history of a flounder Tarphops oligolepis (BLEEKER) in the western Wakasa Bay and adjacent waters, the Japan Sea was studied.
General morphology of the postlarvae and juveniles ranging from 2.69mm to 10.90mm BL, was described. The characteristic features of this fish in early larval stages were elongations of the dorsal fin rays and distributional patterns of melanophores on the body surface, forming faint transverse bands.
Judging from gonad index and occurrence of larvae, actual spawning may occur during the period from May to July in the western Wakasa Bay.
Postlarvae and juveniles were caught mainly in nearshore region, and this area may be their spawning and nursery ground.
Analyses of the gut contents showed that the food organisms are copepod nauplii in early postlarvae, copepods in late postlarvae and mysids in juveniles, respectively.
The early life histories of the three bothid flatfishes, Paralichthys olivaceus, Pseudorhombus pentophthalmus and Tarphops oligolepis, in the western Wakasa Bay were compared, and relation between morphological differences and ontogenetic migration in pelagic phase was discussed.View full abstractDownload PDF (570K) -
Akira OCHIAI, Kazuma MUTSUTANI, Susumu UMEDA1983 Volume 49 Issue 4 Pages 541-545
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe cultural and spawning experiments of jack mackerel Trachurus japonicus (TEMMINCK et SCHLEGEL) from our hatchery were carried out from April 18, 1980 to July 16, 1981. The growth of the cultured fish was considerably high and the fish reached 17.4cm in fork length and 67.0g in weight in average at the end of its first year. The optimal temperature for the growth is ascertained to be between 18° and 26°C. All males are found to be spermiating without hormone injection in April and June, 1981. In some females the ovaries are well enlarged, with maturing eggs deveploped to primary or tertiary yolk stage in May and June, 1981. It is suggested that the cultured fish attained sexual maturity at least at a length of about 15cm in fork length. Twenty six females and 25 males are treated with gonadotropin in May 26, 1981, and placed in a circular tank of about 6 ton capacity at the beginning of the spawning experiment. At the water temperature of about 21°C, spawning began at 32:30 hr, and completed at 39:45 hr after receiving gonadotropin. The fertilized eggs incubated at the water temperature of abuot 21°C began hatching at about 34:40 hr after first spawning.View full abstractDownload PDF (343K) -
Yukimasa ISHIDA, Syoiti TANAKA1983 Volume 49 Issue 4 Pages 547-553
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSJuveniles less than 20mm total length (TL) were collected by hand beam trawl from July through October. Small-sized fish 30 to 50mm TL began to be recruited to the catch by boat seine from August through September. Although they showed annual fluctuation of pattern in the length frequency distribution, small-sized fish grew up to large-sized fish from 50 to 70mm TL in spring through summer of the next year. A negative relationship between population density and growth in weight was observed. The spawning season is estimated to extend from May through September judging from the seasonal change of a gonadsomatic index. Some small-sized fish recruitd in summer also had well developed gonads. The rate of group maturity for females at around the end of August or time of recruitment showed annual variation, and was lower in 1977 when population density was higher than in other years.View full abstractDownload PDF (441K) -
Yuzuru SUZUKI, Takashi HIBIYA1983 Volume 49 Issue 4 Pages 555-559
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIncreased vascular permeability in acute inflammation of fish was measured quantitatively by the blueing method. Carp were inflamed by the injection with 10% formalin into the pectoral fin, and pontaminskyblue was injected intravascularly through the canulae implanted to the dosal aorta. The dye was adsorbed onto blood plasma proteins. And the extent of blueing in inflamed tissue which give an indication of the degree of protein leakage, was estimated by extraction of the dye. Acute exudation of blood plasma was observed only in the first 30 minutes just after formalin treatment, but it did not last longer.
The amount of exudation was estimated by the other method, comparing the water contents of control and of inflamed pectoral fins. In formalin inflammation, acute exudation and fairly rapid absorption was observed. In croton oil inflammation, the response appeared more slowly and continued much longer. Diphasic reactions, early and late, were not seen in either case.
By the latter method effect of mammalian chemical mediators, histamine, serotonin and kinin were tested. But these were not effective on vascular permeability of fish.View full abstractDownload PDF (323K) -
Michiya MATSUYAMA, Shuhei MATSUURA1983 Volume 49 Issue 4 Pages 561-567
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn a previous paper, we described that oocytes developed asynchronously and only the first batch was ovulated. This paper presents the change in number and size of eggs during ovarian maturation and ovulation of the amphidromous type Ayu Plecoglossus altivelis captured in Chikugo River, Fukuoka Prefecture, Japan.
On the basis of oogenetic stages of the most advanced group of oocytes in the ovary, the course of ovarian maturation was divided into six phases. GSI values corresponded to the maturity stages of this group. Number of eggs of the first batch decreased with the deveplopment of ovary. The number of ripe eggs laid in a spawning season corresponded to number of eggs of the first batch at phase III. Number of yolk-less eggs which are at the peri-nucleolus stage and less advaced stages decreased markedly with the development of ovary and increased again at the stage just after ovulation. It seemed that there was a multiplication period of oogonia before or after ovulation.View full abstractDownload PDF (486K) -
Sanae HASEGAWA, Tetsuya HIRANO, Norishige KUNIYA, Yoshiya ABE, Keiji S ...1983 Volume 49 Issue 4 Pages 569-575
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOsmoregulatory ability of the anadromous and the landlocked forms of the ayu Plecoglossus altivelis was examined by following changes in plasma ion concentrations after transfter to sewater. On direct transfer from fresh water to seawater, 90% of the juvenile ayu weighing 0.5-5g died within 24h. When they were transferred to seawater after acclimation in 50% seawater for 3 days, the plasma Na concentration increased significantly after 1 day and decreased to a seawater-adapted level after 3 days. When the seawater-adapted fish were transferred back to fresh water, the plasma Na concentration decreased to the freshwater-adapted level within 6h. There was no difference in seawater of freshwater adaptability between juvenile fish of the anadromous form and those of the landlocked form. In contrast, juvenile rainbow trout weighing 8-10g were unable to adapt to seawater, even after acclimation in 50% seawater for 3 days. The seawater adaptability of the landlocked ayu was also maintained after gonadal maturation. The intrinsic euryhalinity of the ayu might have played an important role in differentiation of this species and acquisition of migratory habits of descending to the sea.View full abstractDownload PDF (477K) -
Tatsuaki MAEDA, Toyomi TAKAHASHI, Motokazu UENO1983 Volume 49 Issue 4 Pages 577-585
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe authors examined the biological data of adult Alaska pollack Theragra chalcogramma (PALLAS) caught by bottom gill net at three definite stations (100m, 200m and 300-350m depth) in the adjacent waters of Funka Bay from February 1974 to February 1975.
1. In the spawning periodg gonads of males ripen earlier than femals and males migrate from the feeding grounds (300-350m depth) to the spawning grounds (near 100m depth) before females. Most of the males remain in the spawning grounds through the period, while females migrate to the spawning grounds i the order in which gonads ripen and leave soon after spawning. Consequently, males abund remarkably in the spawning grounds. There is no feeding activity among the spawners.
2. In the transitional period from the end of spawning period to the beginning of feeding period, almost all the fish disperse chiefly to the offshore area in search of their food organisms.
3. In the feeding period, adult fishes concentrate in the bottom layer water of the Oyashio current (300-350m depth) which is abundant in Euphausiids and Amphipods. But, in 1974 when these food organisms were scarce fishes migrated to area near 200m depth and fed chiedfly on the young Alaska pollack and shrimp.
4. In the transitional period from the end of feeding period to the beginning of spawning period, adult fishes mature rapidly. Males begin to migrate to the spawning grounds and females continue light feeding activity in the feeding grounds.View full abstractDownload PDF (628K) -
Hiroshi UEDA, Yoshitaka NAGAHAMA, Fumio TASHIRO, Laurence W. CRIM1983 Volume 49 Issue 4 Pages 587-596
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSChanges in plasma androgen and gonadotropin (GTH) levels were correlated with changes in morphology of testes and pituitaries during precocious sexual maturation in the male amago salmon Oncorhynchus rhodurus parr. Although testicular development in precocious male parr began in early summer (June), dramatic increases in gonadosomatic indices occurred during the month of September. Active spermiation began in early October and continued into early November. Plasma androgen levels began to increase sharply in late August, coinciding with the onset of rapid testicular development. High circulating levels of androgen (15-18ng/ml) were maintained during September, followed by a distinct drop in early October. Plasma GTH levels were relatively low during the major part of the spermatogenesis period and increased during the spawning period, followed by a sharp decline in spent males from mid-November.
There were two types of pituitary cells, globular cells and vesicular cells, which were found to be closely associated with testicular development. The globular cells were characterized by having a few large globular inclusions as well as numerous fine granules. These cells increased remarkably in number during the active spermiation period, coincident with the rise in plasma GTH levels, suggesting that the globular cells are the major source of GTH. The chromophobic vesicular cells appeared most active during the later stage of testicular development. It is concluded from these observations that pituitary GTH plays a major role in precocious sexual maturation of the male amago salmon parr. This action of GTH may be mediated by the steroidogenic tissue of the testes.View full abstractDownload PDF (2029K) -
Hiroaki HASHIMOTO1983 Volume 49 Issue 4 Pages 597-599
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwo subpopulations of young sandeel Ammodytes personatus were found off Tottori Pref. by examining a combination of the vertebral count and gill raker number. These two subpopulations reveal differences also in size and stomach contents. Coexistance of two subpopulations in this area seems to be closely related to the oceanographical conditions: eastward Tsushima Warm Current encountering southward Japan Sea Central Water in spring.View full abstractDownload PDF (220K) -
Kenji CHIBA1983 Volume 49 Issue 4 Pages 601-610
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwo experiments were conducted to clarify the effect of dissolved oxygen on the growth of young sliver bream (body weight 3-15g).
In experiment I, DO level was controlled by N2 gas bubbling, and young fish were cultured under various DO levels. The growth rate decreased in the thanks in which DO level was less that 60% and the food conversion efficiency decreased remarkably at the DO level less than 40%. Little growth was observed at DO level 20%.
In experiment II, young fish were cultured under various DO levels and waste substance levels. The experiment comprised of 5 tanks which were arranged in duplicate series. Regard-less of the waste substance accumulation, retarded growth was observed only in those thanks in which average DO level was less than 50%. It was considered that the DO decrease was more effective on the growth that the accumulation of NH4-N (1.5ppm).
From the results of these experiments, DO level 60%, at water temperature 23.5-29.8°C, could be considered the minimum level for young silver bream to maintain a normal and healthy growth.
Four young silver bream culture in ponds, adequate attention should be paid to DO level rather that on the accumulation of waste substance; and DO level should be kept at more than 60% in air saturation.View full abstractDownload PDF (670K) -
Michiko TANIGUCHI1983 Volume 49 Issue 4 Pages 611-615
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSBased on previous finding that the incidence of disease was higher in yellowtail fed with defrosted food that in those give unthawed food, the feeding experiment on frozen diet was tried using various food fishes. Yellowtail accepted the unthawed food without any trouble. The property of the frozen diet to float on water was useful to locate left over food and to monitor activity of the fish. The experimental group fed with unthawed diet had the best growth rate and feeding efficiency compared with the other experimental groups given thawed diet. The survival rate was also very high in the group given unthawed food. Washing treatment and adding ethoxyquine or vitamin mixture to the diet were also effective comparet with the control group. Even if old frozen food was used, the group fed with unthawed diet had better growth rate and feeding efficiency than the other groups. Based on the results, it is recommended that feeding yellowtail on unthawed diet should be applied as a practical control for bacterial infection and water pollution in yellowtail culture.View full abstractDownload PDF (357K) -
Hyoji KUSAKA, Hiroshi NARITA, Keiko IWATA, Shizuyuki OHTA1983 Volume 49 Issue 4 Pages 617-620
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFlavor component of ascidians Cynthia roretziv DRASCH which were cultivated in Okirai Bay, Iwate, Has been investigated by gas liquid chromatography (GLC). About 4g of pale yellow flavor concentrate (FC) was obtained by steam-distillation from 30kg of minced fresh ascidians which were removed from their tests. GLC peaks attributable to unsaturated compounds were estimated by compariso with those of the hydrogenated FC.
Major constituents of the FC weve identified by IR, GC-MS and retention time, comparing with those of reference standards as follows, n-decadienol (32.6%), n-octanol (31%), n-octenol (15.4%), n-decenol (9%) and n-heptenol (8%). The iodine value 169.3 of the FC by the Wijs method was consistent with the value 171.6 calculated on the basis of the above composition.
It should be noted that the unsaturated alcohols were the major constituents in the FC.View full abstractDownload PDF (247K) -
Masayoshi OKANO, Fumie MIZUI, Yumiko FUNAKI, Takaaki ARATANI1983 Volume 49 Issue 4 Pages 621-626
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe present paper describes the seasonal variation of constituents such as sterol, hydrocarbon, higher fatty acid, and phytol fractions from Enteromorpha prolifera (MÜLLER) J. AGARDH (Chlorophyceae) which grow profusely at tidal zone in the Seto Inland Sea of Japan.
E. prolifera is characterized by higher contents of 28-isofucosterol (Z-24-ethylidene-cholest-5-en-3β -ol) as the major component in the sterol fraction throughut the year. This content increeased to its maximum level level in the winter months (propagative season) and then gradually decreased to its minimum level in the early spring months when the alga was at the mature stage. The contents of 24-methylenecholesterol (24-methylenecholest-5-en-3β -ol) and cholesterol (cholest-5-en-3β -ol) were consistently low in all seasons.
cis-7-Heptadecene was characteristically present in apreciable amounts, along throughout the year with lower contents of n-eicosane and phytol
The C18-unsaturated fatty acids were the major components in the higher fatty acid fraction in allseasons. These contents increased to the maximum levels in the early spring months (decay season).View full abstractDownload PDF (396K) -
Yumiko MATSUDA1983 Volume 49 Issue 4 Pages 627-632
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe influence of relative humidity (RH) on the quality change of lyophilized carp myofibrils during storage was estimated. Carp myofibrils were ground with polyphosphates and sucrose. The mixtures were freeze-dried at 60 or 35°C, ground into powder, and stored in desiccators and over saturated salts slurries and other materials with constant relative humidities. Moisture sorption isotherms for lyophilized carp myofibrils were determined by drying those stored for ten days in desiccators. At suitable intervals, aliquots of the powders were taken out and examined as to the quality, using as indexe: solubility in 0.6 M KCl, relative viscosity and ATPase activity of 0.6 M KCl extractable protein, and the total ATPase activity.
The results for powders with RH 10 to 98% stored at room temperature of 15 to 18.8°C for 6 months indicated that the lower the humidity, the better was the quality retention of the powder. In RH 10 to 15% there was no significant difference in the effect of RH. A second experiment studied RH 0 to 20% the qualities of powders stored in the RH below 15% were acceptable for 1 year at 20°C. The last experiment was concerned with RH 0 to 20% at a high temperature of 35°C; here, storing in th RH below 10% gave the qualities which were acceptable for a storage period of 6.5 months. Thus it was shown that the lyophilized myofibrils were stable even in the moisture range of free from monolayer moisture.View full abstractDownload PDF (419K) -
Takeshi YASUMOTO, Yasukasu OSHIMA, Masahiro TAJIRI, Yuichi KOTAKI1983 Volume 49 Issue 4 Pages 633-636
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwelve species of crabs collected at Okinawan coral reef were tested for the presence of paralytic shellfish toxins. In addition to the previously known three xanthids, the following seven species were newly found to the toxic: Neoxanthias impressus, Actaeodes tomentosus, Eriphia scabricula, Pilumnus vespertilio, Schizophrys aspera, Thalamita sp. and Percnon planissimum. Toxic genera covered four families: Xanthidae, Majidae, Portunidae and Grapsidae. Toxin compositions of E. scabricula, P. vespertilio and Thalamita sp. were characterized by the predominance of saxitoxin and neosaxitoxin over gonyautoxins, similarly to that of Zosimus aeneus.View full abstractDownload PDF (226K) -
Reiji YOSHINAKA, Hideki TANAKA, Mamoru SATO, Shizunori IKEDA1983 Volume 49 Issue 4 Pages 637-642
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAn elastolytic enzyme, designated as elastase B, from the pancreas of the catfish Parasilurus asotus was characterized and compared with the well-established elastase from the porcine pancreas.
The approximate molecular weight of catfish elastase B was estimated to be 26, 000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of catfish elastase B was similar to that of porcine elastase. Elastase B had high activities toward elastin, casein, hemoglobin, and Suc-(Ala)5 -NA, but very low activities toward Tos-Arg-OMe and Bz-Tyr-OEt. Catfish elastase B was strongly inhibited by DFP, PMSF, elastatinal, soybean trypsin inhibitor (Kunitz), and chicken ovoinhibitor.
These findings indicate that catfish elastase B belongs to the group of serine proteinases and is closely related to porcine elastase.View full abstractDownload PDF (400K) -
Atsushi WAKAMEDA, Ken-ichi ARAI1983 Volume 49 Issue 4 Pages 643-650
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTo study the effect of neutral salts on myosin B (MB) of white croaker and carp, MB (pH 7.0) was kept at a fixed temperature in the range of 2-30°C with various concentrations (0.3-2.0M) of KCl of NaCl and the change in the viscosity was measured as a function of the lapse of reaction time.
The decrease in the viscosity number (Zη=1nηr/C) was found to proceed in two first-order steps: an early fast decrease, followed by a slow decrease. As the concentration of salt (M) in the medium increased, the rate (K) for the decrease in Zη as well as the extent (ΔZη) of viscosity decrease both increased.
The relationship between the salt concentration (M) and the K values revealed that white croaker MB was more susceptible to salt than carp MB. A similar trend was also observed between the salt concentration (M) and ΔZη. In addition, the increasing rates of K and of ΔZη were found to be the order of LiCl, NaCl, from the highest to the least.
The high salt-induced decrease in viscosity of MB occurred quickly even at low temperatures, around 2-15°C, while the inactivation of its Ca-ATPase by salt was detectable only at temperatures, around 2-15°C, while the inactivation of its Ca-ATPase by salt was detetable only at temperatures higher than 20°C.View full abstractDownload PDF (622K) -
Taishi UEDA, Motokazu NAKAHARA1983 Volume 49 Issue 4 Pages 651-654
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTo know the effect of chemical forms of Co on its accumulation by marine fish, the accumulation and excreton of Co by the organism were observed using 60CoCl2 and 57Co cyanocobalamin. The biological half life of the long component of 60CoCl2 was 166 days, and that of 57Co cyanocobalamin was 43 days at 15°C. The concentration factor at the steady state was calculated as 32 for 60CoCl2 and 3 for 57Co cyanocobalamin. The higher concentration factro of 60CoCl2 than 57Co cyanocobalamin was characteristic in comparison with other marine organisms. The distribution (%) of 57Co cyanocobalamin in the liver was three times higher than that of 60CoCl2.
It was estimated from the gel filtrations on Sephadex G-75 and Sepharose CL-4B that 60Co combined with five constituents in the liver of Acanthopagrus schlegeli and Chasmichthys gulosus, and with two constituents in the liver of Girella punctata. The gel filtration profiles of 57Co cyanocobalamin in the liver of C. gulosus was similar to those of 60Co. These constituents were considered to be protein by referring to the optical density at 280nm. A constituent with a molecular weight of 2×105 wh ch combined with Co was characteristic in livers of fish among the marine organisms.View full abstractDownload PDF (242K) -
Shugo WATABE, Junichi MARUYAMA, Kanehisa HASHIMOTO1983 Volume 49 Issue 4 Pages 655
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (87K) -
Kouichi OHWADA, Nobuhiko TANAKA, Motohiko SUGIYAMA1983 Volume 49 Issue 4 Pages 657
Published: April 25, 1983
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (1175K)
- |<
- <
- 1
- >
- >|