NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 49, Issue 8
Displaying 1-27 of 27 articles from this issue
  • Tokuo SANO, Hideo FUKUDA, Nobuaki OKAMOTO, Fumiyo KANEKO
    1983 Volume 49 Issue 8 Pages 1159-1163
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In January 1981, we isolated a virus in RTG-2 and CHSE-214 cells from a mandible tumor of a cultured yamame Oncorhynchus masou at Niigata Prefecture, Japan. Cross neutralization test indicated that this virus was apparently closely related to a herpesvirus.1) We designated it Yamame Tumor Virus (YTV).
    Intraperitoneal injection of YTV was lethal to 5-month-old yamame fry weighing an average of 2.9g, with cumulative mortalities 65% and 30% with 103 and 102 TCID50 per fish, respectively. However, the cumulative mortality in 5-month-old chum salmon (O. keta) fry weighing 4.7g, inoculated intraperitoneally with the same doses mentioned above, was 15% and 0%, respectively.
    The mandible or premaxillae of the survivors of both species of fish developed tumors in about ten to 13 months after inoculation. It took more than ten months ot develop visible tumors at 14-16°C of water temperature. The spontaneous tumor from which YTV was isolated and the tumor reproduced by artificial infection of YTV were histopathologically very similar to a basal cell epithelioma.2)
    This is the first report which establishes the oncogenicity of a herpesvirus isolated diretly from a fish tumor by fulfilling the Rives' postulates.
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  • Keiji HIROSE, Yoshitaka NAGAHAMA, Shinji ADACHI, Katsumi WAKABAYASHI
    1983 Volume 49 Issue 8 Pages 1165-1169
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The time course changes in serum concentrations of gonadotropin (GtH), 17α-hydroxypro-gestrone and 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-diOHprog) during LH-RH-induced final oocyte maturation and ovulation in gravid ayu Plecoglossus altivelis were examined. The fish underwent GVBD one day following a single intraperitoneal injection of emulsified LH-RH and ovulated in 2 days. A slow and moderate increase in serum levels of GtH occurred 1-3 days after injection. The LH-RH treatment caused a prompt increase in hte serum levels of 17α-hydroxypro-geterone and 17α, 20β-diOHprog concomitant with final oocyte maturation and ovulation. The levels of 17α, 20β-diOHprog remained high for 1-3 day after injection, followed by a sharp decrease at 5-7 days. The elevation of 17-hydroxyprogesterone, which is a precursor for 17α, 20β-diOHprog occurred earlier and persisted longer than the changes in 17α, 20β-diOHprog. Thus, the changes in serum levels of these steroids during final maturation and ovulation are medizted through the secretion of pituitary GtH. These findings suggest that 17α, 20β-diOHprog is the major ovarian mediator of LH-RH-induced oocyte maturation in ayu.
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  • Michiko TANIGUCHI
    1983 Volume 49 Issue 8 Pages 1171-1174
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    To clarify the process of streptococcicosis of yellowtail, the symptom, pathogen incidence, and mortality were recorded with time elapse using peroral inoculation of the bacteria. The incubation period was usually one to 3 days, rarely within one day. The pathogen increased tremendously to more than 104 cfu/g on hte 4th or 5th day after inoculation, when viable bacteria were counted in the blood and liver of the inoculated fish.
    The symptoms usually appeared more than 3 days after inoculation except redness inside the operculum, which often appeared within a day after inoculation. The seriously infected and dead fish were usually observed on the 5th day after inoculation. There was little time lag between the spread of pathogen to the different organs and the appearance of symptoms. The progress of the disease was very variable. Some inoculated fish died within a few days after the symptoms appeared while others died soon after the symptoms appeared. Some inoculated fish also recovered before showing serious symptoms.
    The inoculated fish reared under good environmental conditions and given high quality food were not seriously infected. They tend to be more resistant to the disease, however, it was still not possible to prevent the occurrence of the disease. On the other hand, the inoculated fish reared in poor environmental conditions and fed with low quality food soon died after the appearance of serious symptoms.
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  • Takafumi ARIMOTO, Yoshihiro INOUE, Michio OGURA
    1983 Volume 49 Issue 8 Pages 1175-1181
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The present paper describes the variation of catch set-line fisheries with special reference to the operation time, analysing the results of experimental fishings conducted at Sagami Bay in 1979 and 1980. The diel variation of hooking rate (Total catch/hook) was obtained, showing higher values at dawn and dusk, and lower values in the daytime as well as at night. Catch composition, however, was different in each fishing operation time. The diel variation of hooking rate was discussed for each species, so that several patterns of its variation could be classified. One is the nocturnal, that indicated the high values of hooking rate at night, for brown hakeling Physi-culus maximowiczi, bermuda catfish Promethichtys prometheus, and common Japanese conger Conger myriaster. The other is the diurnal and divided into two further types: The hooking rate of silvergray seaperch Malakichthys griseus was high at dawn and/or dusk, the same was true for Japanese bluefish Scombrops boops and Hilgendorf saucord Helicolenus higendorfi. While, pacific pomfret Brama japonica showed high values of hooking rate in the daytime.
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  • Gunzo KAWAMURA, Takashi SHIMOWADA, Katsuya BANNO
    1983 Volume 49 Issue 8 Pages 1183-1186
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    To obtain some fundamental knowledge regarding the vision of fishes attracted to a fish lamp, the retinomotor response and the effect of general and local lighting on the shape discrimination of dark adapted bluegil sunfish Lepomis machrochirus were examined in a tank. Histological observations showed that 10 minutes after the onset of general lighting the retina was still dark adapted. A remarkable change in the thickness of pigment and cones took place between 10 and 15 minutes, but the retina was not light adapted within this period. The dark adapted fish which was pretrained to discriminate shapes, discriminated the shapes successively displayed within 7 minutes and the shapes simultaneously displayed within 9 minutes. The dark adapted fish did not respond to the shapes and retreated from the discrimination point when the fish's head was illuminated with local light. From the results obtained, it is presumed that while the fishes attracted to the fish lamp can recognize to some extent the shape of fishing gear even if their retian is not light adapted histologically, the effect of the lamp on the vision of fish is extremely harmmful when the lamp is close to fish's visual direction.
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  • Makoto TERAZAKI, Munehico IWATA
    1983 Volume 49 Issue 8 Pages 1187-1193
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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    Feeding habits of chum salmon fry was studied, using the samples collected from 7 locations in the Otsuchi Bay during the period from 1978 to 1980. Amphipoda, Appendicularia, Chaeto-gnatha, Cladocera, Copepoda, Euphausiacea, Polychaeta, crab and shrimp larvae, fish eggs and larvae, and land insects were found in stomachs of chum salmon fly. Chum salmon fly generally consumed food organisms that were abundant in their habitats. Epibenthic crustaceans were their important food organisms in the inner part of the bay but zooplankton near the mouth of the bay. The mean feeding rate was 1-4%. Feeding rate of chum salmon fry captured near the mouth was larger than that of fry collected from the inner part of the bay. A remarkable difference was recognized in the feeding rate between day and night. Chum salmon fry did not actively feed during the night. Condition factors of released chum salmon fry was about 0.7 and they increased during their stay in the Otsuchi Bay. Large-sized chum salmon fry living near the mouth of the bay had condition factors of more than 0.9. This suggests that chum salmon fry had good environmental conditions during their stay in the Ostsuchi Bay after the seaward migration.
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  • Takehiko IMAI
    1983 Volume 49 Issue 8 Pages 1195-1198
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    This report clarifies the surface areas of knotted netting, especially on knot which plays an important role for carrying out fluid-dynamical studies on netting fabrics.
    In this study, the so-called “knot” is defined as a tied portion of the twine including short legs, provided that the legs are cut veritcally along a normal direction under a condition of stretched mesh.
    The nettings used are made of Weaver's knot and reef knot, with the three-ply z-twist polyethylene twine.
    The surface area of a knot is obtained by measuring the circumference of cross-section of a paraffin-knot-model, with curvimeter.
    Results obtained are as follows:
    1) Netting surface area Ssw of Weaver's knot is represented as following experimental equation.
    Ssw=2ij{2Q(l-3.87d)+31.67d2}+31.67d2(i+j)
    2) Netting surface area Ssr of the reef knot is represented as following experimental equation. Ssr=2ij{2Q(l-3.42d)+26.31d2}+26.31d2(i+j)
    Where each symbol is as follows, d: diameter of twine, i: mesh numbers along the twinewise direction, j: mesh numbers along the normal direction, l: length of a leg, Q: circumference of a twine, Ssr: surface area of the (i×j) meshes reef knot netting, Ssw: surface area of the (i×j) meshes Weaver's knot netting.
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  • Usio SIMIDU, Won Jae LEE, Kazuhiro KOGURE
    1983 Volume 49 Issue 8 Pages 1199-1203
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    For seawater Samples from various environments, viable bacterial counts obtained by various plate count techniques were compared with each other and with direct viable counts and total counts. Of the three plate count methods, i.e., spread plate, filter and pour plate methods, the filter method gave the lowest number of colony forming units. Average nercentages of number of colony forming units obtained by the filter method to the number obtained by the spread plate method ranged from 5.2% for samples from Tokyo Bay to 26% for samples from open seawater of the Pacific Ocean and the South China Sea. The pour plate method using agar media of low gelling temperature yielded about 90% of the number of colony forming units obtained by the spread plate method. The colony forming units in the pour plate method decreased in number about 20% when the temperature of molten agar media was raised from 37°C to 40°C. The pour plate method with agar media of low geling temperature has an advantage over the spread plate method when water samples from open seas. in which low numbers of colony forming units can be expected, are to be examined.
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  • Toshiaki OHSHIMA, Chiaki KOIZUMI
    1983 Volume 49 Issue 8 Pages 1205-1212
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Phosholipids of the muscle of fresh skipiack Euthynnus pelamis were analyzed by HPLC, GLC, and TLC. The contents of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) in skipjack muscle were extremely high, though earlier workers had reported that the contents of LPC adn LPE produced enzymatically in the fish muscle were considerably low and did not increase during frozen storage. The accumulation of LPC and LPE in the skipjack muscle might be elucidated by either saying that the activity of phospholipase A2 is higher than that of lysophospholipase or that the lower pH of skipjack muscle influences the activity of lysophospholipase; the optimum pH of lysophospholipase in fish muscle is known ot be around 7.6.
    The fatty acid compositions of phosphatidylcholine (PC), LPC, and free fatty acid were analyzed. Percentage of C22:6 was considerably high in the PC, while extremely low in the LPC. These results seem to indicate either that most of the PC having C22:6 located on the α-position of glycerol is not attacked by the action of phospholipase A2 or that the LPC having C22:6, if produced from PC by the action of phospholipase A2, is attacked preferentially by the action of lysophospholipase to librate C22:6.
    The possible fatty acid combinations of the PC remaining in hte skipjack muscle were also presented.
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  • Toshiaki OHSHIMA, Shun WADA, Chiaki KOIZUMI
    1983 Volume 49 Issue 8 Pages 1213-1219
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Changes in phospholipids of skipjack muscle during ice storage were studied using GlC, HPLC, and TLC. During ice storage, the contents of phosphatidylcholine (PC). phosphatidylethanolamine (PE), sphingomyelin (SPM), and phosphatidylserine (PS) decreased, while those of lysophosphatidylcholine (LPE), and free fatty acid (FFA) increased. These results indicate that enzymatic hydrolysis of phospholipids occurred, since the content of triglyceride did not change.
    LPC and LPE were found in the muscle of skipjack analyzed the day after the day after the catch at though less in quantity, but increased during ice storage for 2 days, and the decreased. The maximum amount of LPC was 99.5 mg/100 g. In the fatty acid composition of LPC, percentages of C16:0, C18:1, C18:0 and C16:1 were relatively high, while that of C22:6 extremely low. Though Percentage of C22:6 was highest in the fatty acid composition of PC. In addition, percentage of the PC having two molecules of C22:6 as constituent fatty acids increased gradually during ice storage. These results suggest that the PC having C22:6 on α-postition o fglycerol was scarcely attacked by the action of phospholipase A2 in the skipjack muscle.
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  • Kenzo TOYAMA, Masakazu HOSHI, Yukio KAMIOKA, Tateo OHTAKA, Makoto YAMA ...
    1983 Volume 49 Issue 8 Pages 1221-1231
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Amino acid composition of fish meals and fish solubles prepared from putrefied sardine or mackerel was analysed in comparishon with that obtained from relatively fresh fish by the pilot plant at almost the same ocnditions. These estimations were carried out in parallel with the preceding work on the same samples used for the histamine determination.
    With the putrefaction of raw fish, the amounts of total, individual, and essential amino acids decreased in the fish meal fraction, while those in the fish solubles fraction and its related products, whole meals, increased, A similar tendency was also observed in the total nitrogen content of the same samples These results indicate that the degree in the detruction of fish proteins, reflects upon the distribution pattern of the nitrogen compounds, especially amino acids, between solid fraction (meals) and liquid fraction (solubles) at the cook/press procell. Consequently, the effect of putrefaction of raw fish was not such a serious problem on the quality of fish meals. On the solubles and also on the whole meals, however, deterioration gave an antagonistic effect on the qualities by favourable increase in amino acids content and undesirable incrase in histamine and volatile basic itrogen as reported before.
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  • Fujio NISHIOKA, Ritsu MACHIDA, Yutaka SHIMIZU
    1983 Volume 49 Issue 8 Pages 1233-1238
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The dolphinfish myosin (purity 92%) was compared with respect to the kamaboko-forming ability atomyosin (myosin moiety 70%), myofibrils (myosin moiety 72%) and minced meat (myosin content 32%) prepared from the same specie. Kamaboko jelly from the dolphinfish myosin was diferet with regard to others, in that it was transparent, light blueish and much more elasticl The strength of myosin jelly was 3.0, 2.6 and 44 times more than that of actomyosin, myofibrils and minced meat jelly having the same moisture content (86%), showing that the kamaboka-forming ability of those materials are solely dependent on their myosin moiety content, CM. It was found that the tensile strength of the jellies can be expresed as a function of CM, S=k•CMn (k, n are constants), while hte breaking extension is decreased with the concentration of moieties other than myosin.
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  • Yutaka SHIMIZU, Fujio NISHIOKA, Ritsu MACHIDA, Chau Ming SHIUE
    1983 Volume 49 Issue 8 Pages 1239-1243
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The salt-added myosin sol (90-92% moisture, 2.5% NaCl, pH 6.8-7.0) prepard form ten species of teleosts and rabbit were examined for their gelaiton propertiees, and both suwari, setting phenomenon occuring at 30-40, and modori, gel-disintegration phenomenon occuring at around 60, were found in the gelation. The setting and disintegration properties of myosin were both highly specific with specific with species: rabit and dolphinfih were difficult-setting and difficult-disintegrating species: yellow mackerel and oval-filefish were difficult setting and easy-disinte-grating species: flying fish, flathead, grubfish and nibe-croaker and filefish a little esy-setting and easy-disintegrating species. Such characteristics in the gelation pattern of the myosin solproves to strongly resemle to theat of the meat paste prpared form the corresponding secies.
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  • Reiichiro HIROTA, Junko ASADA, Shizuko TAJIMA, Yoshiaki HIRANO, Motoo ...
    1983 Volume 49 Issue 8 Pages 1245-1247
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The mercury contents of copepods collected at 13 stations in three inland sea regions along the coasto of Western Japan were measured. The Mercury contents of Western Japan were measured: The Mercury contents of copepods measured in this survey were highest in Minamata Bay (over0.4 ppm in total mercury, on a dry weight basis) and lowest in the Seto-naikai (under 0.08ppm). Such results sugest and that the Minarmata Bay, where methyi-mercury was discharged about twenty-five years ago, is still polluted by mercury and that the environ-ment of the Seto-maikai is not polluted by mercury.
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  • Reiichiro HIROTA, Junko ASADA, Shizuko TAJIMA, Motoo FUJIKI
    1983 Volume 49 Issue 8 Pages 1249-1251
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Acartia clausi were maintained at various densities (30-1, 100 individuals per liter) in filtered sea water containing mercuric chloride or methylmercury chloride of several concentrations (0.05, 0.1, 0.3 and 0.5ppb). After 24 hours, the uptake of inorganic mercury by the copepods was nearly constant (biconcentration factor=approx. 7, 500 on a ddry weight basis), regardless of both mrcury concentration and the desity of copepods. In contrast, bioconcentration of methylmercury fluctuated markedly; it showed a high negative correlatio (r=-0.9310) with the density of copepods, though there was no relation to the mercury concentration in the sea water. Such results srggest that methylmercury in the sea watre is accumulated rapidly by Acartia clausi, and that the concentration of methylmercury in copepods and sea watre reached equilibrium within the experimental period (24 hours).
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  • Hiroshi DUMAGAI, Kiyoko SAEKI
    1983 Volume 49 Issue 8 Pages 1253-1256
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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    The muscles of wild and cultured puffers Fugu rubripes of different sizes were analyzed for heavy metals such as cadmium (Cd), lead (Pb), copper (Cu), zinc (Zn), manganese (Mn), nickel (Ni), and dcobalt (Co) by atomic absorption spectrometry after wet digestion. Each heavy metal content was on a level similar to that of young fish in both wild and cultured state and hardly and change was found with growth rgard to heavy metal content. Acompaison of ench heavy metal content of cultured puffer with that of its diet, sardine; showed that there is no bioaccumulation of these heavy metals through food chain in cultured puffer. The concentraiton of each metal in wild puffer was 0.025±2.2ppm for Cd, 0.24±0.02ppm for Pb, 0.29±0.03ppm for Cu, 8.5±2.2ppm for Zn, 0.505±0.005ppmfor Mn, 0.12±0.02ppm for Ni, and 0.055±0.003ppm for Co. These metal concentrations of wild puffer were almost the same as those of cultured puffer in spite of the intake of different diets, and there was no problem with regard to food hygiene about these hevy metal concentrations.
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  • Takao OJIMA, Kiyoyoshi NISHITA
    1983 Volume 49 Issue 8 Pages 1257-1264
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Mg-ATPase of the striated adductor myosin of akazara scallop Chlamys nipponensis akazara was studied at various temperatures and calcium ion concentrations. At lower temperatures than 15°C, the myosin ATPase activit was totally sensitive to Ca2+. Above 20°C, however, the activity was increased at Ca2+ concentrations lower than 10-6 M, resulting in the loss of Ca-sensitivity. This phenomenon was attributable to th edissociatoin of th eregulatory light chains from the myosin molecule during heat treatment. The dissociation rate and extent of the light chains were affected by several lfactors; the temperature and ionic strength mixture, th concentration of divalent cations such as Ca2+ and sr2+, and the concentration of F-actin. The heat-treated myosinwas able to regain its full content of regulatory light chain as well as its full Ca-sensitivity of the Mg-AATPase activity and of the superprecipitation ability of actomyosin. The heat treatment was demonstrated to be useful as a new device to desensitize akazara myosin, even in presence o th ephysiological concentration of Mg2+.
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  • Shigeru NAKAJIMA, Toru TAMIYA, Takayuki AKHANE, Juichiro J. MATSUMOTO
    1983 Volume 49 Issue 8 Pages 1265-1271
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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    Lactate dehydrogenase (LDH) of garp muscle is inactivated during extraction and succeeding purification prceses. Inactivation is found also in dilutiion process which is done prior to the activity determintion. When LDH was extracted and purified with media containing sodium glutamate, the obtained LDH samples demonstrated higher activity than those prepared with water media. By using medis containing 0.1M sodium glutamate in place of the water medis of KORNBEG'S purification method, th specific activity of the LDH sample was raised mardidly. Dilution with glutamate containing media also raised the activity. These effects were attriuted to the preventioin of the inativation of LDH. Similar preventive effect was tested on several media. namely eter, 0.15M NaCl, 5mM DTT, 0.25M sucrose, 10% glycerol an d0.1M sodium glutamate. Cros effects of these media were also tested. Glutamate was found most effective, sucrose the next, and glycerol third, while other media were of little effect or none. The resultant LDH samples were as active as over 200 units permg protein. The LDH activity was determined by KUBOWITZ's method.
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  • Kinue KOYAMA, Tamao NOGUCHI, Atsushi UZU, Kanehisa KASHIMOTO
    1983 Volume 49 Issue 8 Pages 1273-1279
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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    A total of 119 specimens of th exanthid crab Zosimus aeneus were collected from Ishigaki Island (102), Hachijo Island (1), the Bonin Islands (5), and Ceby island in th ePhilippines (11) from 1979 through 1982, and asayed for toxicity by mouse asaay.
    The results showed that almost all the speciment were toxic, except a single specimen from Ishigaki Island. A remarkable individual variation of toxicity was observed among th especimens collected form a given locality, even mong those from a narrow area. The local variation in toxicity was also large. Ishigaki specmens generally showed higher toxicity scores than those from other localities, the highest score rcorded being 16500 MU/g.
    There was a tendency that the toxicity is higher in specimens less than 60g in body weight than in larger ones. No significant differences in toxicity were observed between sexes and between years of collection.
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  • Takeshi AGUCHI, Munehiko TANAKA, Kosaku SUZUKI
    1983 Volume 49 Issue 8 Pages 1281-1283
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In order to elucidate the mechanism of “himodori”, the thermally induced disintegration of fish meat gel, some experiments were performed with the myosin prpared from the oval filefish Navodon modestus. Oval filefih myosin displayed thermally induced gelation curver similar to those of meat paste. Addition of protease inhibitors to myosin sol preened to some extent th himodori disintegration, though the preventive effect vanished gradually at 60°C. On the other hand, the addition of n-butyl alcohol showed a marked inhibitory effect, depressing himodori completely. It was discused from these results that the formation of a specific netwark structure in gel is a prerequisite for himodori disintegration, which is in turn promoted by the action of proteases.
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  • Masayo OKUZUMI, Masaishi AWANO
    1983 Volume 49 Issue 8 Pages 1285-1291
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In the previous papers, the authors repored that a new type of histamine-forming bacteria (tentatively named N-group bacteria) was isolated from a common a mackerel, and that this group of bacteria were also found in Pacific saury and sardine, and gew well at 2.5 storage of thee fish samples.
    The present study was undertaken to obtain more information as to seasonal variation in the mumber of N-group bacteria in seawater and on the fisher skins and intesines. N-group bacteria werre found most abundantly in the sewater during the late autumn and winter, and it is considered that this group of bacteria strikingly decreased in number when the water temperature rose above 22°C.
    The majouity of the fresh fish samples had less than 100 organisms of organisms of N-group bacteria per cm2 of skin; and especially in the summer samples, they were below 10 orgainisms. The number of this group of bacteria increased at 2.5storage of the fish samples and eached to 107-108 level in a few samples. This group of bacteria were found in all of the intesine amples, where their number ranged from 103 to 108 per gram.
    A selective enrichment medium with PH 4.5 was devised for detection and isolation of N-group bacteria.
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  • Yumiko MATSUDA
    1983 Volume 49 Issue 8 Pages 1293-1295
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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    The basic studies on the preparation and prservation of lyophilized carp myofibrils have been carried out. In the present study its practical application was conducted. At intervals of a month aliquot of the lyophilized carp surimi powder containing 5% ucrose and 0.2% polyphosphates was taken out and examined as to kamaboko-forming ability and protein properties.
    Immediately after freeze-drying the kamaboko-forming ability of the powder determined by jelly strength, bending test value, organoleptic gel strength, experssible water and hunter whiteness was maintained as the same high level as control. And during the subsequejt storage for 5 months at the relative humidity of 15% and temperature of 15, bending test value which was regarded as a good index of kamaboko-forming ability was maintained at high level of AA through the storage period while the others decreased progressively. In the protein propeties examined using as indexes solubility in 0.6M KCL, krlative viscosity and ATPase dactivety of 0.6M KCL extractable protein and the toal ATPase activity, except relative viscosity immedialy after freeze-dryig, there was not any significant diference in particular during the freeze-dying and subsequent storage.
    It was concluded that the fish meat power having kamaboko-forming ability and biological activity was produced successfully, provided that the fish meat was gound with sugar and subsequently freeze-dried completely. The kamaboko-forming ability of the powde was retained fairly well for a long period, provided that the lyophilized fish meat powde is stored at relateve humidity of below 15% and temperature below 15°C. Inert gas packing should be introduced, if the fishes have high lipid content or the lipids are susceptible to oxygen.
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  • Akira HIRAISHI, Kazuaki SAHEKI, Susumu HORIE
    1983 Volume 49 Issue 8 Pages 1297
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • Takao MATSUNO, Takashi MAOKA
    1983 Volume 49 Issue 8 Pages 1299
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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  • Koertaro TAKAHASHI, Tsugihiko HIRANO, Koichi ZAMA
    1983 Volume 49 Issue 8 Pages 1301
    Published: August 25, 1983
    Released on J-STAGE: February 29, 2008
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  • 1983 Volume 49 Issue 8 Pages 1304a
    Published: 1983
    Released on J-STAGE: April 22, 2008
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  • 1983 Volume 49 Issue 8 Pages 1304b
    Published: 1983
    Released on J-STAGE: April 22, 2008
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