巻号一覧
50 巻, 12 号
選択された号の論文の29件中1～29を表示しています
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松浦 修平, 濱野 龍夫1984 年 50 巻 12 号 p. 1963-1968
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe Japanese mantis shrimp Oratosquilla oratoria usually lives in the mud bottom. This species excavates a U-shaped tunnel with a sarge and a small opening on a tidal flat; the length of burrow is proportional to occupant size. Artificial burrows of various diameters and lengths were made of polyvinyl chloride pipes and used to study this selective habit in large aquaria. The results showed that the species had a significant preference on the basis of diameter of pipe, but not for pipe length above a lower limit. These facts and behaviour in the artificial burrow suggest that a normal life-cycle is dependent upon habitation of a burrow of compatible size. An artificial burrow of favourable size permits feeding, spawning, etc. and increases survival of mantis shrimp reared in the laboratory.抄録全体を表示PDF形式でダウンロード (857K) -
濱野 龍夫, 松浦 修平1984 年 50 巻 12 号 p. 1969-1973
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe spawning and egg mass nursing behaviour, together with the change in external appearance of the egg mass, of the mud-dwelling Japanese mantis shrimp Oratosquilla oratoria were studied using artificial burrows in aquaria.
This species usually spawns in a supine posture in the burrow maintaining this posture with the back of abdomen and exopods of uropods against the wall of burrow for almost 3 h. The newly extruded egg mass is amorphous, but the female shrimp compacts it and then shapes it into a thick disc within 1 h and to a thin disc having a marginal turnup within 24 h of spawning. All maxillipeds excluding the raptorial claw are used for the nursing behaviour, i.e. spreading and folding the egg mass, so it is teased out and becomes a tuft shape after 3 days or so.
The second gee laying occurred in 2 females about 40 days after the first spawning of each individual at 25°C, and this fact suggests that O. oratoria may spawn more than once in a spawning season in natural warm waters.抄録全体を表示PDF形式でダウンロード (1997K) -
川村 軍蔵, 津田 良平, 熊井 英水, 大橋 伸一1984 年 50 巻 12 号 p. 1975-1980
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe visual cell morphology in 2-90mm long larvae and juveniles, and adult of the red sea bream Pagrus major was examined histologically. The visual system of the larvae is poorly developed at hatching, but becomes functional in 36 hours. At this time, the eyes are well pigmented, cones are formed, and optic nerves connect with the optic tectum. The visual cells of larvae smaller than 10.6mm are all single cones. At 11mm TL, the single cones fuse to form twin cones, and rods appear at the same time. Red sea bream shifts from a pelagic to a benthic habitat when about 12-15mm long. The observed increase in visual sensitivity at 11mm appears to be a preadaptation to life at greater depths. Likewise, the specialized retinal region for acute vision shifts from temporal to dorso-temporal at a length of 30mm, implying a change in visual axis from fore to lower-fore direction, and an adaptation to the change in diet from planktonic to benthic animals.抄録全体を表示PDF形式でダウンロード (1423K) -
加藤 進, 大熊 和行, 広部 宏, 清水 千秋, 前川 徳昭1984 年 50 巻 12 号 p. 1981-1989
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーSeveral statistical approaches (pattern analysis, cluster analysis, and ratio matching) were applied for classification of marine environmental quality of ISE bay based on similarity among data on sea water parameters (W. T., pH, DO, COD, Sal., DIN, PO4-P, and chl-a) collected during march, 1977 through March, 1979.
Presence of two typical water masses having similar marine environmental quality were found from these analyses.
One water mass which showed relatively independent quality upon seasonal and yearly fluctuations occupied a larger area from the coast line of Tokoname-Noma to the center of the bay.
The other one which showed seasonal and yearly fluctuations occupied only small area along the cosat of Mie. Moreover, the regions determined to have marine environmental quality by the ratio of matching method coincided relatively well with the regions where red tide occurred.抄録全体を表示PDF形式でダウンロード (514K) -
舞田 正志, 塩満 捷夫, 池田 彌生1984 年 50 巻 12 号 p. 1991-1998
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe hemochemical assessment was made on the yellowtail Seriola quinqueradiata in July and September, 1983. The specimens examined were one year old fishes weighing about 2, 300g. They were fed on frozen sardine (groups I and II) or thawed sardine (group III), and reared in seawater of temperature about 28°C. In order to minimize the stress effects of handling, fish were angled, and then blood samples were collected by cardiac puncture with heparinized syringe within three minutes. Using these samples, various plasma constituent levels or enzyme activities were determined.
In group I, some Pathological changes were observed in intestine (reddening), peritoneum (adhesion) and opercle inside (reddening) possibly resulted from feed trouble. Various blood constituent levels or enzyme activities remained within the normal limits, suggesting that feed trouble was slight. In group II, in which a few dead fish was found every day, pathological changes were similar to those in group I, but the symptoms were more serious than in group I. Alkaline Phosphatase (EC 3.1.3.1.) activity and inorganic phosphorus level were lower, whereas chloride level was higher than those of group I. Reduced bony growth and feed trouble were seen. In group III, that was in worse condition, pathological changes were observed in various organs, and were parasitized by Benedenia and/or Caligus. Levels or activities of hematocrit, hemoglobin, urea-N, total cholesterol, aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.1), and alkaline phosphatase were lower than those of group I, whereas chloride level was higher. These results suggest that this group suffered from malmutrition, hepatic malfunction and low metabolic rate.抄録全体を表示PDF形式でダウンロード (517K) -
澄川 精吾, 石松 維世1984 年 50 巻 12 号 p. 1999-2004
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe canal system and digestive processes of the digesive gland of Cyclina sinensis were studied in a low intertidal population. Specimens were collected from a low-tide level at the estuary of the Tsuri-River, Fukuoka Prefecture, in February, August and November 1981. The sections were examined after staining with haematoxyline and eosin, Azan or Halmi's trichrome.
The gland consisted of branched canal system, which contained four kinsd of canals, main and lateral ducts, secondary duct and blind-ending tubule. The main ducts communicating with the somach were two on each side in this species. The tubule consisted of two kinds of the cells, digestive cell and darkly-staining basophyl cell.
The darkly-staining cells were always present at three crypts in cross section of the tubule, and these three groups of darkly-staining cells extended continuously through the length of each tubule to meet at the apex. The cell was smaller and appeared triangular in section, bore a long beating flagellum.
The digestive cell was larger, granulated and lacked cilia or flagellum. The cell varied in shape from flattened to cuboidal or columnar in accordance with the state of intracellular digestion. Differences in the morphological appearance of digestive gland tubules were attributed to the changes within the digestive cells, which make up the tubules. These morphological variations could be recognized as five distinct tubule types (holding, absorption, digestion, breakdown, final stage of breakdowm).抄録全体を表示PDF形式でダウンロード (1467K) -
小川 和夫1984 年 50 巻 12 号 p. 2005-2011
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーA description is given of the development as well as some notes on the biology of Benedenia hoshinai OGAWA, 1984, a monogenean parasite of the Japanese striped kniifejaw Oplegnathus fasciatus (Japanese name: “Ishi-dai”). The oncomiracidium, 0.3-0.4mm long, attaches itself directly to the body surface and fins of the host, Adult worms are about 3.5mm long or over, being most abundant on the tail fin, dorsal soft fin rays and postero-dorsal side of the body. The hard parts (two pairs of hamuli and the supplementary pieces) show a clear relative growth to the parasite body length, and continue to grow even in adults. The number of worms on the host sharply decreased as they grew bigger than 1.5mm long, suggesting that most small worms detached themselves from the host before reaching that size.抄録全体を表示PDF形式でダウンロード (464K) -
木村 晴保, 宗景 志浩1984 年 50 巻 12 号 p. 2013-2018
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーEquations for surface waves and interfacial waves and their relations in a basin with depth iscontinuities were given by Lamb, Le mehaute, Takano, and Rattray.
If the length of the bay is much larger than the width, and the curvature of the bay aixs is small, it is possible to consider that the surface long waves, i.e. the tidal waves and the interfacial long waves generated in these basins can be treated as a one dimensional problem.
The bay can be divided into many rectangular segments as shown in Fig. 1, and each of the divided segments corresponds to any of the model segments as shown in Fig. 2. The basic equations for the surface and the interfacial long waves in the model canals are given on the basis of Lamb and Takano's theory. The numerical computations for the surface long waves and the interfacial waves generated in a two layer basin with variation in depth and width. can be carried out by using the basic equations in the model canals.抄録全体を表示PDF形式でダウンロード (391K) -
荒井 克俊, 内藤 文隆, 佐々木 英世, 藤野 和男1984 年 50 巻 12 号 p. 2019-2023
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーProbable usefulness of induced gynogenesis followed by diploidization has been discussed as a part of breeding technologes for genetic improvement of aquacultural organisms. The present study examined sets of conditions of treating spermatozoa by ultraviolet ray irradiation for effective induction of gynogenesis in the pacific abalone Haliotis discus hannai on the basis of observations on fertilization rate of the eggs, survival rate and chromosome constitutions of the zygotes.
An aliquot of sperm suspension, placed in a plastic petri dish ion-sputtered in advance, was exposed to irradiation of ultraviolet ray of 42 erg per mm2 per s for varying durations. Immediate after the irradiation, the treated spermatozoa suspension was added to egg suspension at room temperature for fertilization. Irradiation of spermatozoa by ultraviolet ray of the above intensity for 30 to 60s successfully induced gynogenesis, indicating the high frequency occurrence of the haploid zyogtes consonant with the recovery in survival rate of zygotes, called Hertwig effect.抄録全体を表示PDF形式でダウンロード (1037K) -
塚本 勝巳1984 年 50 巻 12 号 p. 2025-2030
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe object of this study is to determine the role of red and white muscles during swimming activity of the yellowtail Serila quinqueradiata using both anatomical and electromyographical methods.
The distribution of red and white muscle was observed in yellowtails 33-42cm in fork length. The proportion of red muscle in the transverse section increased toward the tail and showed a sharp increase at the caudal pit reaching a value of 56.3%. This, as well as the structure of oblique tendons in the horizontal septum, suggests that the red muscle is important in tail movement althought it occupies only 9% by weight of the whole lateral muscle.
Muscle activities during exercise were detected through electromyography in circ. 50 fish 36cm in fork length. The red muscle was always active, whereas no electrical activity could be detected from the white muscles at velocities below a certain threshold (e.g. 61cm/s) peculiar to the individual. Above the threshold velocity, however, the white muscles became active and the amplitude of bursts of discharge from these muscles increased with increase in swimming speed. These results apparently indicate that there is a simple dichotomy in muscle recruitment in the yellowtail. It seems that the red muscle is mainly used for moderate swimming at low speeds while the white muscles are used for emergencies such as attack, escape or jumping.抄録全体を表示PDF形式でダウンロード (474K) -
塚本 勝巳1984 年 50 巻 12 号 p. 2031-2042
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーBased on electromyograms from the red and white muscles of the yellowtail Seriola quinqueradiata swimming in a water tunnel, the power output of each muscle was estimated. Maximum amplitude (A) and duration (D) of 10-20 consecutive bursts of discharge in the recording were measured at various swimming speeds for 30 fish of circ. 36cm in fork length. The work of a burst was obtained from the product of A×D2, and the power (P) derived from n bursts for a period of T seconds was given by; P∝∑ni=1Ai•Di2/T Power outputs of each muscle obtained from the above expression were then calibrated by the measurement of oxygen consumption. The power output of the red muscle increased with speed to a maximum (75.0×105erg/s/fish) at 99cm/s and then decreased. The white muscles began to work at 65cm/s and their power increased with speed showing a rapid increase above 99cm/s. These two speeds, 65 and 99cm/s, seem to represent critical points of change in muscle power output and are named the “Ignition Point of White muscle” (IPW) and the “Saturation Point of Red muscle” (SPR), respectively. Below IPW, power is supplied only from the red muscle, between IPW and SPR, from both red and white muscles, and over SPR, mainly from the white muscle. The interrelationships between these three ranges and some physiological characteristics of the fish are discussed, e.g., heart rate, fatigue curve, oxygen consumption and lactate accumulation.抄録全体を表示PDF形式でダウンロード (1033K) -
仁木 弘, 加藤 恒夫, 五十嵐 清一郎1984 年 50 巻 12 号 p. 2043-2047
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーPeruvian hake meat was compared with Alaska pollack meat to understand the properties of meat.
Actomyosins were prepared from Peruvian hake and Alaska pollack. ATPase activity, ATP sensitivity, super precipitation, intrinsic vicosity and SDS gel filtration pattern were measured for these actomyosins. There were not many big differences between Peruvian hake and Alaska pollack.
The results suggested that Peruvian hake meat is normal except that it contains a protease in the water soluble fraction.
Therefore, in order to remove the protease from Peruvian hake meat, the following method was developed. Peruvian hake meat was washed 5 times by usual surimi-making method, the washed meat was dissoloved in 0.45M salt solution and the salt solution was diluted with water.
By this method, the myofibril structure was broken, the interior of the muscle structure was washed, the protease was removed from the muscle and the purified surmi was got.
The purified surimi has a good kamaboko-forming property and can be used as raw material of AFPP.抄録全体を表示PDF形式でダウンロード (360K) -
山口 邦子, 豊水 正道1984 年 50 巻 12 号 p. 2049-2054
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーIn lipid oxidation during cold storage of fish, the authors found that TBA/kg skin of fatty fish was always higher than that of lean fish; whereas TBA/10g lipid in the skin of lean fish was frequently higher than that of fatty fish. With the purpose of finding the cause of high TBA/10g lipid in the skin of lean fish, we examined the prooxidant in the skin of fishes.
The extracts from skins of Pacific mackerel and Plaice accelerated oxidation of linoleate enzymatically. The prooxidant was found to be dioxygenase which had an optimum temperature at 25°C and a pH value of 13 and required Co2+ as a stabilizer and contained Fe3+ in the molecule.
The activity of dioxygenase per g of defatted skin was high in the skin of fatty fish and low in that of lean fish but the activity per g of lipid turned out to be the reversal of this order. At the same time the residual activity in the skin of lean fish was higher than that of fatty fish after the storage at -5°C.
From these results, the authors concluded that the high TBA/10g lipid in the skin of lean fish during cold storage was caused by the dioxygenase.抄録全体を表示PDF形式でダウンロード (449K) -
石井 孝典, 西田 時次, 御堂河内 奨, 時武 哲也, 金子 哲男, 竹花 秀夫, 石黒 鉄郎1984 年 50 巻 12 号 p. 2055-2059
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーIn studies on the concentration of cadmium in invertebrates, especially limpets, it is required to know how many samples are to be averaged.
In studies on Cd-binding proteins, it is necessary to know in what organ Cd is concentrated.
Short-necked clams Tapes japonica were kept in sea water which contained stable Cd and radioactive 115mCd.
The amount of Cd accumulated by a clam was measured with NaI (Tl) scintillation counter one by one, without sacrificing the clam.
The distribution of Cd in the body of a clam was made clear by “Macroautoradiograph” which was seldom used on limpets.
The results obtained were as follows: 1) The accumulation of Cd by clams differs considerably from each other. 2) A main Cd-accumulating organ in a clam is the Bojanus' organ, as well as the mid-gut gland which accumulated Cd considerably.抄録全体を表示PDF形式でダウンロード (1343K) -
磯 直道, 水野 治夫, 斎藤 隆英, 大関 文照, 王 慥1984 年 50 巻 12 号 p. 2061-2064
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe stress-relaxation experiment was done with the raw and hated yellowtail meat. The sample with skin exhibited higher values of both elasticity and viscosity than the sample without skin. The rheological parameters depended on the freshness of the sample (rigor mortis). The elastic modulus, viscosity, and the 1st and 2nd relaxation time decrease at first until ca. 40°C of the heating temperature, and then increase as the heating temperature increase. The heating temperature dependency of the meat was discussed by comparing it with the results of carp meats.抄録全体を表示PDF形式でダウンロード (287K) -
外山 健三, 星 昌和, 高嶋 裕一, 佐藤 秀一, 竹内 俊郎, 渡辺 武1984 年 50 巻 12 号 p. 2065-2075
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーAvailability of Japanese domestic scrap meal as a protein source in fish feed in place of white fish meal was investigated by chemical analyses such as proximate and amino acid compositions. The samples were scrap meal with fish solubles produced from raw materials consisting of different levels of round fish and waste of canning materials, and those from fish offal and viscera of fish processing without fish solubles. They were collected monthly from two factories in Choshi district, Chiba pref., from May 1981 to April 1982, and their nutritional quality was examined by comparison to that of white fish meal and round fish meal.
The quality of domestic scrap meal fluctuated from lot to lot, depenaing upon the constituent of raw materials used. Scrap meal prepared mainly from round fish was found to be nutritious, because of their high protein and low ash levels, together with a high EAA index. Consequently, the nutritional quality of this meal was almost comparable to that of white fish meal; whereas those manufactured from deteriorated round fish or waste product of canning were far inferior to white fish meal in quality.抄録全体を表示PDF形式でダウンロード (710K) -
佐藤 秀一, 竹内 俊郎, 渡辺 武, 星 昌和, 外山 健三1984 年 50 巻 12 号 p. 2077-2083
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーProtein quality of various kinds of scrap meal, sampled regularly once a month from two meal factories, was compared with that of white fish meal by determining growth, feed effciency, digestibility of protein, PER, and NPU in rainbow trount and carp. The various scrap meal used contained 49-68% crude protein and 12-20% crude lipid.
The nutritional quality of scrap meal was found to vary from month to month in manufacturing of the samples, although there was no marked difference in the quality as a protein source between both the fish species. Some of the scrap meal showed high values for PER and NPU compared to the white fish meal in both rainbow trount and carp, indicating the availability of these meals as a protein source in fish diets. These scrap meals were found to be high in content of crude protein and low in content of crude ash and moisture, together with a high digestibility of protein. It was also found that it was possible to substitute 10-20% of white fish meal in fish diets with the scrap meal of the lowest quality.抄録全体を表示PDF形式でダウンロード (423K) -
小川 廣男, 荒木 繁, 大房 剛, 鹿山 光1984 年 50 巻 12 号 p. 2085-2089
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーFollowing the previous works on the safe storage of ascorbic acid in hoshi-nori (dried laver, Porphyra yezoensis), we tried to find the cause of the destruction of ascorbic acid; which reaction does occur in hoshi-nori during storage, an enzymatic or a chemical oxidation?
I. Ascorbic acid was decomposed with both aqueous extracts of hoshi-nori and yaki-nori (toasted laver) as much as an enzyme free control at 37°C.
II. A reducing velocity of ascorbic acid in hoshi-nori agreed with that in yaki-nori at each moisture condition (aw0.1-0.6) at 20°C.
III. Hoshi-nori was stored at 10, 20, 30 (±0.5)°C under the conditions ofaw≈0 to 0.6 for 8 weeks. The destruction of ascorbic acid could be treated as a first order reaction. The ratio of the rate constants (k) every 10°C corresponded to 2 or 3 at each aw's. ARRHENIUS plots (ln k vs. T-1) gave essentially straight lines. Apparent activation energy for the destruction of ascorbic acid (Ea) was no change below a monolayer moisture content (nearly aw 0.2) and 78kj/mol of Ea at aw 0.6 was the same order as the case of the destruction in an enzyme free model system reported by LEE or LABUZA.
Therefore, we concluded from the experiments described above that ascorbic acid in hoshinori, possibly in yaki-nori, during storage was destroyed mainly by a chemical or non-enzymatic oxidation.抄録全体を表示PDF形式でダウンロード (331K) -
大島 敏明, 和田 俊, 小泉 千秋1984 年 50 巻 12 号 p. 2091-2098
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーEnzymatic hydrolysis of phosphatidylcholin (PC) in skipjack flesh during storage at -20°C for 16 weeks was studied by following the changes in PC composition on the basis of their fatty acid compositions. Major PC in skipjack flesh were those having fatty acid combinations of (C16, C22), (C22, C22), (C18, C22), (C20, C22), (C16, C18), and (C18, C20). Percentages of the PC having fatty acid combinations of (C22, C22), (C18, C22), and (C20, C22) incereased gradually during storage, while that of (C18, C22) decreased distinctly. These results indicate that the PC in skipjack flesh are preferentially hydrolyzed by endogenous lipolytic enzyme system during storage at -20°C. The molecular species of the PC preferentially hydrolyzed were the same as those in the ice-stored skipjack flesh as previously reported. This suggests that the preferential hydrolysis of PC in skipjack flesh occurs regardless of storage temperature.
On the other hand, lyso-form phospholipids such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine accumulated in skipjack flesh during storage. The maximum amount of LPC was 89mg/100g after a 6-week storage period.抄録全体を表示PDF形式でダウンロード (525K) -
銭 重均, 成田 弘子, 奈良 正人, 野口 玉雄, 丸山 純一, 橋本 周久1984 年 50 巻 12 号 p. 2099-2102
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーParalytic toxicity was detected in a small gastropod mollusk, “araregai” Niotha clathrata. The toxin was partially purified from digestive glands of the gastropod by a method which essentially consisted of activated charcoal treatment, followed by chromatography on CM-Sephadex C-25 and Bio-Rex 70.
Araregai toxin thus purified was indistinguishable from tetrodotoxin in thin-layer chromatographic and electrophoretic behaviors. In addition, gas chromatography-mass spectrometry of the alkali hydrolyzate of araregai toxin indicated the presence of quinazoline skeleton specific to tetrodotoxin.
Araregai toxin was identified as tetrodotoxin from these results.抄録全体を表示PDF形式でダウンロード (1047K) -
加藤 登, 橋本 昭彦, 野崎 恒, 新井 健一1984 年 50 巻 12 号 p. 2103-2108
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe frozen surimis of Alaska pollack, white croaker and tilapia were ground with 3% salt. The meat pastes were submitted to proceed the setting, so-called “suwari”, at a fixed temperature in the range of 10-45°C. During the setting, a portion of the meat pastes were taken out to assess the jelly strength.
As temperature increased, the apparent rate for the setting was enhanced for all fish species. The temperature dependence of the rate for setting, however, was remarkably different among them; the setting of the paste from Alaska pollack was rapidly proceeded even at lower temperature but the similar setting rate of the paste from tilapia was obtained only at higher temperature. The setting of the paste from white croaker proceeded at moderate rate.
ARRHENIUS plots for the setting rate of meat pastes from three fish species showed that they have significantly different slopes, approached each other at higher temperatures and gave an identical setting rate at around 49°C.
The thermodynamic activation parameters for the setting rates, estimated from the corresponding ARRHENIUS plots, were found to be increased from cold water to warm water fish, although the proportions of ΔH≠ and ΔS≠ varied considerably but that of ΔG≠ somewhat among fish species.抄録全体を表示PDF形式でダウンロード (511K) -
藤田 眞夫, 伊藤 慶明1984 年 50 巻 12 号 p. 2109-2115
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーELLMAN method was modified as follows. To 3ml of sample, 2ml of 0.2M phosphate buffer (pH 6.5) and 0.5ml of 0.1% DTNB solution (pH 6.5) were added. The mixture was incubated at 40°C for 10 min, the absorbance was determined at 412nm. HABEEB method was modified as follows. To 3ml of sample 1ml of 10% sodium borohydride was added. The mixture was incubated at 40°C for 30 min and adjusted to pH 6.5 with 1ml of 0.5M phosphate buffer and stood for 5 min at room temperature. Sulfhydryl groups in the solution were determined.
The sulfhydryl groups and disulfide bonds in the fish meat which was heated at 80°C upon addition of KBrO3 to the meat were determined as follows. To 1.5ml of samle, 0.25ml of 1.2% L-ascorbic acid solution, 0.25ml of 1% potassium bromide solution and 0.5ml of 10% sulfuric acid were added in order to decompose potassium bromate. After the mixuture was incubated at 40°C for 15 min, and neutralized with 0.5ml of 15% sodium hydroxide. The solution was used for the determination.
It was suggested that sulfhydryl groups in fish meat turn not only to disulfide bonds but also to sulfonic acid groups by potassium bromate. In other words, the decrease in sulfhydryl groups does not permit the idea of the formation of disulfide bonds.抄録全体を表示PDF形式でダウンロード (546K) -
佐藤 繁雄, 土屋 隆英, 松本 重一郎1984 年 50 巻 12 号 p. 2117-2126
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーDispersion profiles of proteins in “muen” (non-salt) and “kaen” (salt added) frozen surimi (fish mince) were examined under electron microscope. The cross sections of the muen surimis gave various profiles but no appreciable difference related with the jelly strength of the drived kamabokos was found. The dispersion profiles of the muen surimis were classfied roughly into following four types: (1) a profile demonstrating well reserved myofibrillar structures in which the M-lines and the Z-bands remain in nearly intact state, (2) a profile demonstrating moyfibrillar structures in which the M-lines are inappreciable and the vague Z-bands are shown, (3) a profile demonstrating parallel streams of fine filaments which are probably assingnable as actomyosin filaments, (4) a profile in which no uniform structure is appeciable. The dispersion profiles in the kaen surimis were similar to those found in kamabokos (cooked jellies) and showed dense or loose matrices of cross-linked filaments. Dilute homogenate suspensions of the surimis illustrated two types of filaments which were probably assignable as actomyosin filaments and as naked F-action filaments beside aggregates of myosin. The grade SA surimis and some grade C surimis demonstrated fine arrowheaded filaments of actomyosin and gave good jellies. Other grade C surimis demonstrated filaments of myosin-poor actomyosin filaments and gave poor jellies.抄録全体を表示PDF形式でダウンロード (4367K) -
万倉 光正, 鹿山 光, 斎藤 滋1984 年 50 巻 12 号 p. 2127-2131
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーThe wax ester hydrolase activities in liver, hepatopancreas, intestine and pyloric caecum of chub mackerel, blue drum, brown sole, flat fish, yellowtail, red sea bream and black sea bream were compared with the lipase and esterase activities. The lipase activity of pyloric caecum was the highest in all tissues used. In the case of esterase, its activity was remarkable in liver or hepatopancreas of yellowtail and black sea bream, and in pyloric caeca of blue drum and brown sole. Wax ester hydrolysis occurred in almost all the tissues and its hydrolase activity in pyloric caecum of chub mackerel was rather high. The results indicate that wax ester hydrolase activity of the fishes which prey on marine organisms containing more amounts of was esters was noticeable. Pyloric caeca, as a source of lipolytic enzymes, play an important role on wax ester metabolism in the same way as liver, hepatopancreas and intestine.抄録全体を表示PDF形式でダウンロード (321K) -
川村 軍蔵, 向井 幸則, 太田 開之1984 年 50 巻 12 号 p. 2133
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (126K) -
柏木 正章, 山田 直博, 岡田 芳和, 中村 総之1984 年 50 巻 12 号 p. 2135
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (107K) -
山内 皓平, 香川 浩彦, 伴 真俊, 笠原 昇, 長浜 嘉孝1984 年 50 巻 12 号 p. 2137
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (124K) -
加納 碩雄, 野口 玉雄, 丸山 純一, 橋本 周久1984 年 50 巻 12 号 p. 2139
発行日: 1984/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (94K) -
1984 年 50 巻 12 号 p. 2141
発行日: 1984年
公開日: 2008/04/22
ジャーナル フリーPDF形式でダウンロード (50K)
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