日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
50 巻, 4 号
選択された号の論文の27件中1~27を表示しています
  • 南 卓志
    1984 年 50 巻 4 号 p. 551-560
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    General morphology of the eggs, larvae and juveniles of a flounder Kareius bicoloratus were described. There is some variation in relationship between stage and body length in larvae, especially at the metamorphosis stages. It may mean the variation in the size at settlement.
    Judging from records of occurrence of eggs, actual spawning period may be from January to February. Annual occurrence of larvae was surveyed by means of underwater fish lamp, and it appeared that the number and periold of larval occurrence at the inner part of Maizuru Bay showed remarkable variations from year to year during 1974-1979.
    Eggs were caught in he offshore region of the mouth of the Yura River, prelarvae were also caught in the same region, early postlarvae were around the mouth of Maizuru Bay, late postlarvae were mainly in the Maizuru Bay, and nearshore region of the mouth of the Yura River. Early juveniles were captured in the water closs to the shore of the Yura River and Maizuru Bay, and these areas may be their nursery ground. These facts indicate that an inshore movement took place during the larval stage.
    Analysis of the gut content showed that the food items were copepod eggs and nauplii in early postlarvae, copepods (mainly Centropages sp.) in late postlarvae, and mysids and amphipods in early juveniles.
    The early life histories of two species, Kareius bicoloratus and Limanda yokohamae, were compared.
  • 竹村 暘
    1984 年 50 巻 4 号 p. 561-564
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The calls of the fresh water goby Odontobutis obscura consisted of a train of about eight pulse sounds, with each lasting for about 9 msec and followed by a silent period of 30-40 msec. Each pulse sound was made of a frequency component of less than 1 KHz. They emitted vigorous calls during the reproductive season, May to July, while the calls were seldom heard in other seasons. No remarkable diurnal variation in the frequency of sound emitting was observed. The calls which are mainly used for threatening invaders, are made by the rubbing of the upper and lower pharyngeal teeth, and amplified by the swim-bladder. The power and the frequency range of a call are limited by the size of swim-bladder, namely the fish size.
  • 会田 勝美, 加藤 禎一, 淡路 雅彦
    1984 年 50 巻 4 号 p. 565-571
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Underyearlings of precocious male masu salmon were castrated in August and then were reared until next April under natural conditions to study the effect of castration on the induction of smoltification.
    Forty seven out of 196 castrated fish which survived until April were completely castrated. Of these fish, larger ones became smolt, whereas smaller ones did not become smolt. Other 149 castrated fish had a small piece of remaining testis and smoltification did not occurr. All the sham operated fish had a small testis and were parr. Plasma androgen levels were low (about 1ng/ml) in both partially castrated and sham operated fish in April.
    These results indicate that precocious maturation inhibits the parr to smolt transformation and plasma androgen concentration of about 1 ng/ml is probably high enough to prevent the transformation. Growth rate is also an important factor in inducing smoltification as well as gonadal immaturity.
  • 今井 健彦
    1984 年 50 巻 4 号 p. 573-576
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The purpose of this report is to determine an experimental equation of the surface area of three-ply z-twisted polyethylene netting twine to analyse the netting resistance of knotted netting.
    To derive semi-empirically an equation from the theoritical value of a twine circumference, it was assumed that the shapes of ply on the vertial cross-section consisted of three elliptic pieces which are in contact with each other. While in this case, a ply of twine was made of polyethylene multi filament yarns, accordingly it was assumed that the shape showed by ellipse was deformed, so it was necessary to measure the twine circumference by means of paraffin twine model, on the vertical cross-section.
    As the result, it was possible to denote the circumference of a twne, “Q”, as the following experimental equation.
    Q=2.64a(E+E')+0.65 Accordingly it was possible to denote the surface area of three-ply twine having the length of “l”, as the following equation.
    Ssl={2.64a(E+E')+0.65}l
    In this case “a” is a long-semi-diameter of ellipse, “E”and “E'” are the values from the second-ary elliptic integral table.
  • 稲掛 伝三, 平野 敏行
    1984 年 50 巻 4 号 p. 577-589
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Between July and September in 1980, four survey cruises were carried out in the fishing grounds southeast of Hokkaido by embarking on a purse seiner with DBT, XBT and echo sounders to observe sardine Sardinops melanosticus (TEMMINCK et SCHLEGEL) school distributions in relation to oceanic front. Fishing information (such as reports of catch, location, time and SST), called QRY by fishermen, were analysed to find the fishing grounds fromation and their fluctuation between July and October from 1977 to 1980. Sarding schools were usually observed and caught in and around a front. Moreover, their distribution changes responded to seasonal variations of the front location. It is hence considered that the front and thermocline position have a considerable impact on sardine distribution.
  • 室賀 清邦, 山野井 英夫, 弘中 善範, 山本 純弘, 田谷 全康, 城 泰彦, 高橋 誓, 花田 博
    1984 年 50 巻 4 号 p. 591-596
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    From 1979 to 1983, detection of Vibrio anguillarum from wild fringerlings of ayu Plecoglossus altivelis were made by selective enrichment in alkaline peptone water and subsequent plating on BTB teepol agar. The detection rate was 0.08%, 2.5%, 6% and 17% from the fingerlings in Lake Biwa (freshwater lake), Lake Hamana (sea-water lake), the coastal sea of Hiwasa, and the estuary of the Hiwasa river, Respectively. It was found that the fingerlings caught in sea water or blackish water harbored V. anguillarum at highter rates than those caught in the freshwater lake, and the pathogen was detected exclusively in the later period of the season for the fingerling collection in both freshwater and marine environments.
    Among 54 isolates of V. anguillarum, 32 isolates were confirmed to be virulent for eels and most of the virulent strais belonged o the serotype J-O-1, J-O-2, or J-O-3. On the other hand, most of the avirulent strains could not be classified into any of the three serotypes.
    Based on these results, it was deduced that V. anguillarum is introduced into freshwater ayu farms through the fingerlings themselves.
  • 藤野 和男, 佐々木 喜代志, 奥村 誠一
    1984 年 50 巻 4 号 p. 597-601
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Importance of removal of recessive-deleterious genes from seedling population for aquaculture was mentioned before. In this context, evidence was provided for the associations between the thermostability variations of enzymes and occurrence of deficient animals of the Pacific abalone Haliotis discus hannai INO according to the theories of population genetics.
    After careful examination of the above evidence together with the discussions in earlier works on inbreeding structure in the wild populations, occurrence of deficient animals, and associations between thermostability variations and temperature-tolerance of animals, a hypothesis on the mechanisms of development of deficient animals was proposed towards establishing a dependable way of detecting recessive-deleterious genes in individual animals of the Pacific abalone.
  • 木島 明博, 藤尾 芳久
    1984 年 50 巻 4 号 p. 603-608
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Average heterozygosity and effective size of individual river populations of chum salmon were estimated by the proportion of heterozygous enzyme loci per individual and from the number of males and females used for artificial fertilization, respectively. The heterozygosity in river populations showed a broad distribution in the range from 0.060 to 0.128, the average being 0.097. The effective river population size also showed a broad distribution in the range from 120 to 38601, the average being 6675. The estimated effective river population size was always smaller than the census number in individual river populations. The value estimated as 35.2% of its census number as the average.
    A significant positive correlation between the average heterozygosity and the effective size of river population was observed. We conclude that the heterozygosity of river population is difined by the effective river population size and the degree of transplantation.
  • 松田 皎, 鈴木 誠, 兼広 春之
    1984 年 50 巻 4 号 p. 609-615
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The rate of remain was defined as an index to represent the performance of mouth of trap model in the water tank experiment of fish behavior. Letting p and q be the rate of entry and the rate of escape respectively, the rate of remain is given by p(1-q). The water tank experiments for the trap model were made by using bitterling Rhodeus ocellatus ocellatus, the values of these rates being examined for various types and sizes of mouth and leader net. The mean value of the rate of remain for the model with single mouth and leader net was larger than that for the model with single mouth and without leader net, but was smaller than that for the trap model with two mouth and leader net.
  • 原田 勝彦, 池田 至
    1984 年 50 巻 4 号 p. 617-622
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Among nine kinds of chemical constituents of lake prawn Palaemon paucidens, fractions of amino acids, volatile bases, betaines and aldehydes showed high attraction index a for oriental weatherfish Misgurumus anguillicaudatus. The attaction indexes a of the two former substances were approximately equivalent to that of the whole extract obtained by the trichloroacetic acid treatment. The amino acids were more effective in attraction than the volatile bases, while the combination of these two constituents showed a collaborative attraction effect.
    Neutral and basic amino acids in the amino acid constituents attracted the fish remarkably at different concentrations and the attraction activity of the latter was higher than that of the former. On the other hand, acidic amino acids were ineffective in attraction. Ammonia and dimethylamine in the volatile bases were more attactive at different concetrations than trimethylamine. Thus, it was conclued that the amino acids and the volatile bases, especially basic amino acids, ammonia and dimethylamine, are feeding attractants for the fish.
  • 原田 勝彦, 松田 英隆
    1984 年 50 巻 4 号 p. 623-626
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The attraction indexes of the amino acids, the lipids, the volatile bases and the organic acids among nine kinds of chemical constituents were higher than those of the dummies used as control, while the indexes of the two former substances were also higher than those of the whole extract. In addition, the index of the amino acids was appreciably higher than that of the lipids. Further-more, in the four chemical constituents described above, the indexes of the amino acids and the lipids were higher at various concentrations than those of the dummies. In this context, it may be said that the amino acids and the lipids are the feeding attractants for juvenile yellowtail Seriola quinqueradiata.
    Among a variety of combinations between one of the amino acids and the lipids, and noe of other consituents, only the combination of the amino acids and the betaines was higher in the index than the amino acids. This indicates that the betaines reinforce collaborately the attractive effect of the amino acids.
  • 柳沢 忠, 橋本 周久
    1984 年 50 巻 4 号 p. 627-633
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Five albumin components designatcd s2e1, s3e1, s3e2, s3e3, and s3e4 were isolated from yellowtail plasma, by combination of Sephadex G-200 gel filtration and starch block electrophoresis.
    Component s2e1 showed a molecular weight of about 130, 000, and was composed of subunits of an approximate molecular weight of 25, 000. The remaining components whose molecular weights ranged from 56, 000-76, 000, were composed of single polypeptide chains. Components s2e1, s3e3, and s3e4 exhibited yellow, red, and orange colors, respectively. when dissolved in water.
    Component s2e1 was featured by a large amount of lipid up to 60%, and also by binding abilities with BPB and bilirubin. Components s2e1 and s3e3 showed methemalbumin-forming and hematin-binding abilities, the latter component being preferential in either ability. Sevin (l-naphthyl N-methylcarbamate) esterase activity was detected in s3e4 alone.
  • 若目田 篤, 新井 健一
    1984 年 50 巻 4 号 p. 635-643
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The denaturation mechanism of carp myosin B with high concentration of NaCl has been studied by measuring the changes in its biochemical properties.
    During the time carp myosin B was kept at 10°C in the presence of 2.0M NaCl (at pH 7.0), a decrease in Mg-ATPase activity, a decline in viscosity as well as in its ATP sensitivity, an increase in EDTA-ATPasp activity, change in the thermal inactivation mode of Ca-ATPase from a single first order process to biphasic first order behaviour, and a release in a large quantity of actin were found to occur, while Ca-ATPase remained at the same activity level.
    By salting out with (NH4)2SO4 in the presence of 5 mM ATP-Mg, myosin was isolated from the NaCl-treated myosin B. The Ca- and EDTA-ATPase activities, actin-activated Mg-ATPase activity and actin-binding ability of this myosin were identical with those of myosin from untreated myosin B. The NaCl-induced changes in the biochemical properties of carp myosin B, Bove mentioned, were fully restored by the addition of F-actin. These results revealed that a considerable portion of F-actin in the myosin B was denatured faster than its myosin portion during the reaction with high concentration of salt.
  • 竹田 正彦, 滝井 健二, 松井 啓二
    1984 年 50 巻 4 号 p. 645-651
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The present investigation was conducted to identify the feeding stimulants for juvenile ell Anguila japonica using a synthetic extract, based on the composition of the marine worm Perinereis brevicirrus. Juvenile eel showed a marked preference for a diet flavoured with the synthetic extract over an unflavoured diet.
    Feeding preference tests for the diet flavoured with one of the three major fractions (amino acids, uncleotides and other compounds) in the extract showed that the amino acids fraction was most stimulatory, followed by the “other compounds” fraction including glycine betaine and maltose, while the uncleotides fraction including adenosine-5'-monophosphate was inactive or repellent. The active constitunet in the amino acids fraction was identified as the fraction glycine, alanine, proline plus histidine whose activity fraction was mixed with uridine-5'-monophosphate, the resulting mixture was found to be more stimulatory than the amino acids fraction or the complete synthetic extract.
  • 奥積 昌世, 山中 英明, 窪塚 照高, 尾崎 寿司, 松原 清
    1984 年 50 巻 4 号 p. 653-657
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    In the previous paper, the authors reported that histamine-forming bacteria found in the fish samples were N-group bacteria, Proteus morganii, P. vulgaris, Hafnia alvei, Citrobacter spp., Vibrio spp., Aeromonas spp. and so on.
    This study was carried out on the changes in the numbers of histamine-forming bacteria on/in common mackerel samples stored at different temperatrues.
    In both cases of the samples stored at 5°C and 10°C, main histamine formers were only N-group bacteria. In the samples stored at 15°C, the formation of histamine might be attributed to the N-group bacteria, although P. morganii increased during the storage. As for the samples stored at 20°C, a great part of histamine at the early stage of the storage might be accumulated by the N-group bacteria and at the later stage by P. morganii. In both cases of the samples stored at 25°C and 30°C, the accumulation of histamine was due to the action of P. morganii to a great extent.
  • 宮田 康夫
    1984 年 50 巻 4 号 p. 659-663
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The concentration of protein from water in frozen surimi manufacturing process from red meat fish, was examined by ultrafiltration memberane system.
    After suspended matters and fats in the wash water were separated by centrifugation for pretreatment, sample volume was reduced by ultrafiltration (UF) bench scale test unit to 1/10 by volume under following conditions, memberane: tubular-type cellulose acetate memberane, effective membrane area: 0.18m2, operating pressure: 0.49 MPa, feed temperature: below 10°C, feed flow rate: 1.8l/min.
    The results showed that about 90% of protain in the feed was concentrated, and protein recovery was independent of primary protein concentration in the feed.
    Protein content in the total solids of the sample solution was increased by memberane process to 90% in the comcentrated solution, and 50% in the feed solution. Such increment showed that selective permeation of low molecular weight matters had occured.
  • 中川 平介, 笠原 正五郎, 辻村 明夫, 明楽 公男
    1984 年 50 巻 4 号 p. 665-671
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The effect of Chlorella-extrac intake on ayu Plecoglossus altivelis was investigated in terms of its physiological merits. Fish of the control group were reared on commercial diet with 6% feed oil for 49 days. In the experimental group, 1% of Chlorella-extract was supplemented to the above diet.
    The Chlorella-extract contributed to neither body weight gain nor elevation of feed conversion effciency. The biological measurements and proximate composition of the muscle and viscera were not affected by the supplement. Serological measurements revealed a significant decrease in serum lipid and albumin levels in the experimental group fish. Similarly, analysis of body lipid revealed lipid class changes in visceral lipid, suggesting that Chlorella-extract contributed to the regulation of lipid metabolism. The experimental group exhidbited a tolerance for hypoxic conditions, as well. Upon starvation of the fish for 21 days, body weight loss was much lower in the experimental group. The muscle and visceral lipid levels decreased in the experimental group as compared to the control group.
    Chlorella-extract can contribute to the mobilization of a lipid reserve and thus, suppress protein exhaustion due to energy metabolism. The necesssity of ingestion of algae in ayu was discussed in terms of both physiological condition and food habit.
  • 坂口 守彦, 河合 章
    1984 年 50 巻 4 号 p. 673-680
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The promotion of anaerobic growth of Escherichia coli by trimethylamine oxide (TMAO) in muscle extracts from fish and squid was investigted. Carp and yellowtail muscle extracts, which had no or extremely little TMAO in concentration, allowed the bacteria to grow rapidly when TMAO was added to the extracts. There was a high correlation between TMAO contents in muscle extracts from several species of fish and from squid and the bacterial growth yield, suggesting that TMAO acts as a growth-promoting factor probably as an electron acceptor during anaerobic respiration of the bacteria. Levels of either total nitrogen or sugars (total free hexose and total ribose) or lactate in the extracts correlated little with the bacterial growth yield.
  • 仁木 弘, 加藤 恒夫, 五十嵐 清一郎
    1984 年 50 巻 4 号 p. 681-687
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The high viscosity of fish meat sol is one of the most troublesome problems in producing Active Fish Protein Powder (AFPP) by spray-dring. The high viscosity of fish meat sol makes it diffcult to use spray-drying itself and to control moisture content and particle size of AFPP. Also, it decreases the yield of AFPP.
    In order to define the reason of high viscosity of fish meat sol, the viscosity and water holding capacity of myofibril sol (Mf sol) prepared from Alaska pollack were investigated. The viscosity of Mf sol was measured by Ostwald viscometer. The water holding capacity (WHC) of Mf sol was obtained by centrifugal method.
    There was a definite relation between the viscosity and WHC of Mf sol. The viscosity of Mf sol was similar to that of actomyosin sol and was different from that of actomyosin in 0.6 M KCl solution. The viscosity and WHC of Mf sol were noticeably influenced by the changing ionic strength (I) of the medium. They showed the lowest value at I=0.1 and remarkably increased with decreasing ionic strength of the medium. The viscosity of Mf sol depended upon its concentration. The limited viscosity (limC→0ηsp/C) equalled 5 dlg-1 and the WHC value was 170g-H2O/g-solid at pH 7.0 and I=0.
  • 仁木 弘, 加藤 恒夫, 五十嵐 清一郎
    1984 年 50 巻 4 号 p. 689-694
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The high viscosity of fish meat sol is noe of the serious problems in producing active fish protein powder (AFPP) by spray drying. In order to find a method which reduces the high viscosity of fish meat sol, the effects of various additives to the viscosity of fish meat sol were studied.
    The myofibril sol prepared from Alaska pollack was used for the experimental model of fish meat sol. Various additives such as K+, Ca2+, Mg2+, ATP, Na4P2O7 and serum albumin were added to myofibril sol. The viscosity (ηSP/C) and water holding capacity (WHC) of myofibril sol were measured.
    From the results of these measurements, the following methods were suggested to prepare the fish meat sol, reduced the viscosity in producing AFPP. They are the method of adding calcium ions to fish meat sol, the method of adding water soluble proteins to fish meat sol and the method of lowering pH in fish meat sol.
  • 山中 英明, 塩見 一雄, 菊池 武昭, 奥積 昌世
    1984 年 50 巻 4 号 p. 695-701
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Changes in histamine contents during storage at 5°C, 20°C and 35°C were examined in the ordinary and dark meats of sardine, saury pike, mackerel, yellowtail, skipjack, big-eye tuna and horse mackerel.
    During storage at 20°C histamine was produced and accumulated at the highest concentrations. In the meats of sardine, saury pike, mackerel and horse mackerel the amouts of histamine increased gradually during storage at 5°C. Histamine formation of dark meat was less than that of ordinary meat at the same storage temperature. During storge at 5°C or 20°C large amouts of histamine were detected sometimes in the fresh meats of sardine, saury pike and mackeerel whose volatile basic nitrogen contents were lower than 20mg/100g.
    The relation between histamine contents and numbers of histamine-forming bacteria was examined in the meat of mackerel during storage at 20°C. At the early stage of storage psychrophilic and halophilic histamine-philic and halophilic histamine-forming bacteria were predominant, i.e., after 2 days of storage when 107-108 of the bacteria were measured, 440mg/100g of histamine was produced. With the lapse of time mesophilic and non-halophilic histamine-forming bacteria incresed in numbers.
  • 児玉 正昭, 緒方 武比古, 川向 和雄, 大島 泰克, 安元 健
    1984 年 50 巻 4 号 p. 703-706
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Analyes of tetrodotoxin content were carried out on the following five species of puffer fish collected from Pacific coast of Tohku area of Japan: Takifugu pardalis, T. poecilonotus, T. porphreus, T. vermicularis, and T. stictonotus. The toxin contents in the muscle of T. poecilonotus were frequently higher than 50 MU/g, with the highest of 350 MU/g and most of the specimens with high muscle toxicity were female. Lethality of the muscle in other four species was less significant, with the exception of a few specimens of T. paradalis. Toxin concentration in the liver and ovary of two species, T. poecilonotus and T. pardalid, were extremely high. The highest value 40, 000 MU/g was found in the liver of T. pardulis. In contrast, the same organs of the deeper dwllant T. porphreus were less to xic. The results of this study point out to the need to pay a special attention for the commercial use of T. poecilonotus.
  • 手島 新一, 金沢 昭夫, 山田 一磨呂
    1984 年 50 巻 4 号 p. 707-712
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    The sterol components of the sponge Acanthella crista-galli were analysed by argentic column chromatography, gas-liquid chromatography (GLC), combined GLC-mass spectrometry, and 400 MHz nuclear magnetic resonance spetrometry. The sponge contained 3β-hydroxymethylA-nor sterols with various side chains from C7 toC11, the major sterol being 3β-hydroxymethyl-A-nor-5α-cholestane and 3β-hydroxymethyl-24-ethyl-A-nor-5α-cholest-7-ene which were not found in natural products. In addition, this sponge contained a Δ15-sterol, 3β-hydroxymethyl-A-nor-5α-cholest-15-ene.
  • 原 彰彦
    1984 年 50 巻 4 号 p. 713-719
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Transferrin (Tf) was isolated from serum of the chum salmon Oncorhynchus keta by neans of salting-out with ammonium sulfate, chromatography on DEAE-cellulose, starch block electrophoresis and gel filtration. The purified Tf showed a molecular weight of 84, 000 by gel filtration, 80, 000 by SDS-PAGE and 70, 000 by sedimentation equilibrium method. The sedimentation coefficient (S020, W) and the isoelectric point (pI) of the Tf were 5.31 and 5.3, respectively. N-terminal amino acid was identified to be alanine. The amino acid composition was also determined. The visible absorbent spectrum of the chum salmon Tf revealed the maximum absorption at 410 nm. The iron binding capacity of the Tf was estimated to be two atoms per molecule. The Tf represented 936-16.8% of the serum protein in the chum salmon. The immunological cross-reac-tivity between the Tf of chum salmon and that of 12 other fishes was tested. Tfs from the family Salmonidae revealed cross-reactivity.
  • 落合 芳博, 渡部 終五, 橋本 周久, 成田 弘子, 浮島 美之, 奈良 正人
    1984 年 50 巻 4 号 p. 721-725
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
    Attempts were made to identify biochemically two gampylid fishes, escolar (“aburasokomutsu”) Lepidocybuum Flavobrunneum and castor oil fish (“baramutsu”) Ruvettus pretiosus, whose muscles contain a large amount of wax esters and hence may casuse a food poisoning when ingested. Myogen fractions were prepared from the ordinary muscles of both fishes as well as various fishes, and subjected to polyacrylamide gel electrophoresis, followed by staining with Coomassie brilliant bule for protein and with reagents for lactate and malate dehydrogenase activities. Results showed that the protein pattern is species-specific enougy to distinguish the two gempylid fishes from any of the other fishes tested. The pattern of either dehydrogenase was found to be less species-specific. However, simultaneous analyses of MDH and protein patterns faciliated the identification of buth gempylid fishes. Cellulose acetate membrane electrophophoresis was also found to be applicable for this purpose, though less satisfactorily.
  • 讃岐田 訓, 岡本 弘子, 人見 宗男
    1984 年 50 巻 4 号 p. 727
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
  • 志村 茂, 井上 潔
    1984 年 50 巻 4 号 p. 729
    発行日: 1984年
    公開日: 2008/02/29
    ジャーナル フリー
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