A study has been made of the capability of the sulfate conjugating enzyme system in the liversouluble fraction from goldfish
Carassius auratus, to phenol and twenty-one phenolic compounds as follows at various concentrations from 0.00067 to 33.3 m
M in reaction media: (1)chlorophenols (
o-;
p-; 2, 4-; 2, 4, 5-; 2, 4, 6-; 2, 3, 4, 6-; 2, 3, 4, 5, 6-), (2) aminophenols (
o-;
p-), (3) nitrophenols (
m-;
o-;
p-; 2, 4-; 2, 4, 6-) (4) polyphenols (resorcinol; catechol; pyrogallol), (5) other phenols (p-cresol; o, ó- biphenol; phenolphthalein; phenol red).
The live-soluble fraction showoed fairly high activities of the sulfate conjugation for half of the tested phenols corresponding to 53-116% of that for phenol (246 nmol/h/g-liver), while the negligible conjugation for less than 5% of that for phenol was observed only in three phenols,
i.e., 2, 4-dinin-tropenol, 2, 4, 6-trinitrophenol and phenol red. On the other had, some phenols which showed low sulfate conjugation among the tested phenols such as pentachlorophenol such as pentachlorophenol strongly inhibited the fulfate conjugating anzyme system.
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