日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
50 巻, 6 号
選択された号の論文の27件中1~27を表示しています
  • 今田 克
    1984 年 50 巻 6 号 p. 931-936
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Lighting conditions in the artificial culture of laver thalli were studied, and the following results were obtained.
    1) Under continuous lighting using white light with constant light intensity, the growth of laver thalli was good at the range of light intensity of 5.5 to 8 KIx and at the lighting period of 8 to 10 hours a day.
    2) When intermittent lighting intensity was varied 1.2 to 8.4 klx or to 200 klx, the growth of laver thalli was almost the same as that in the case of continuous lighting, or showed above 80% of the growth of thalli under the same total light quantity a day as that in the former case.
    3) When the light intensity was about 1 klx, the growth of laver thalli was improved by supplementing some kinds of sugars and salts of organic acids, especially arabinose to the culture media. But under the complete dark condition, the growth of laver thalli was not improved by supplementing sugars and salts of organic acids to the culture media.
  • 中谷 敏邦, 前田 辰昭
    1984 年 50 巻 6 号 p. 937-942
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Development of walleye pollock Theragra chalcogramma eggs fertilized artificially, and their tolerance for thermal chage, were examined. In addition, upward speed and density of the eggs reared at 4°C were measured from 2 cell stage before hatching.
    At 0°C-10°C, high hatching rates of 86-100% were obtained, and declined at -1°C and 13°C. The relationship between the mean days required for 50% hatching (T) and water temperature (θ) is shown by the following equation; T=31.70 exp (-0.12θ). Although the hatching rates of the eggs subjected to a thermal change from 4°C to -1°C at 2 cell stage were low (0-37%), they increased in more advanced stages of development. On the other hand, the hatching rates of the eggs subjected to a thermal change from 4°C to 0°C were high (73-97%) in all developmental stages. Mean upward speed and density of the eggs were 4.9-8.6 m/h and 1.020-1.023 g/cm3, respectively.
  • 小山 純正, 上田 一夫
    1984 年 50 巻 6 号 p. 943-950
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The optokinetic response (OKR) of rainbow trout was investigated using rotating vertical black and white stripes. The rainbow truot showed two kinds of OKR patterns, Sliding-OKR (S-OKR) and Circular-OKR (C-OKR). S-OKR means the response as the fish follows the rotating stripes like a hand of a clock, while C-OKR is the response as the fish follows the stripes along the inner wall of an experimental trough. The S-OKR appeared mainly when the stimulus velocity (the rotating velocity of the stripes) was low (20 deg/s), while the C-OKR was dominant when the stimulus velocity was high (more than 60 deg/sec). With the stimulus velocity within an appropriate range, the fish followed the simulus as fast as the stimulus itself.
    The maximum followable velocity (Vmax) depended upon the stripe width and the illumination intensity, while the minimum followable velocity (Vmin) was not affected by these factors. An abnormal OKR (very rapid response to only one stimulus direction) was produced by unilateral eye-enucleation. The unilateral tectal-ablation had the same effect on the OKR as the contralateral eye-enucleation. These results suggest that visual input from both eyes in necessary for normal OKR and that most of these inputs project to the contralateral optic tectum.
  • 平石 智徳, 山本 勝太郎, 梨本 勝昭, 佐藤 修
    1984 年 50 巻 6 号 p. 951-958
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    This paper attempted to define the approximational function of the swimming form of the rainbow trout. Undulatory movements of the swimming fish staying at the same posittion in a steady flow were recorded by a 16mm movie camera. Two axes were set up to measure the positions of the center of the cross-section. X-axis was in the direction of the steady flow. H-axis was in a direction perpendicular to the x-axis. The measured points were determined by x-coordinate. Mainly, the transformation of the spinal column was measured.
    The amplitude was minimum near the posterior edge of the operculum, and increased in proportion to distance from that point, maximum at the tip of caudal fin. The function of the change of amplitude was indicated by the sum of two exponential functions. The approximational function could be indicated by the product of that function and the function of traveling wave.
  • 野村 和男, 秋山 勲
    1984 年 50 巻 6 号 p. 959-967
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to investigate the autonomic background of behavior in fish, a new method for recording ECG R-spike under unrestrained condition was devised. At first, the positional behavior of bitterling Rhodeus ocellatus ocellatus was studied in a test pool composed of 4 canals arranged in parallel with different water flows, and the condition under which a fish stayed swimming at the same position in a canal was clarified.
    Secondly, in a circulation canal consisting of 4 chambers connected in series, electrodes were set at the bottom on the location where the fish preferred to stay swimming. The R-spike recorded was 30-360 μV in amplitude according to the distance between fish and electrode. It was possible to record simultaneously the opercular movements as well as R-spikes.
    Furthermore, two canal systems of the same size and shape as the original one were set bilaterally that allowed water passage among them through holes. Thus the fish was able to perceive each other through transparent wall with holes by the sense of sight and smell etc., and the social influences of species mates were possible to be studied.
  • 田中 信彦
    1984 年 50 巻 6 号 p. 969-972
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The cell division rates of ten species of attaching diatoms isolated from Ago Bay were measured on the bottom of the culture vessel with running nutrient-poor natural seawater under the illumination of 4000 lx with a dark/light cycle of 10/14 hours at ca. 19°C. The exchange rate of running seawater through the culture vesel affected the cell division rate of attaching diatom. At ca. 4-5 day-1 of exchange rate of seawater, the cell division rates were as follows: Cymbella sp., 0.57 day-1, Stauroneis sp., 0.49; Gyrosigma sp., 0.80; Navicula sp., 0.57; Nitzschia closterium, 1.60; Cocconeis scutellum, 0.86; Melosira sp., 0.27; Grammatophora marina, 0.46; Trigoium formosum, 0.29, and Gomphonema sp., 0.34. The cell division rates obtained in running nutrient-poor seawater corresponded to those in nutrient-enriched medium. The cell division rates of former six species which showed gliding movement and did not from their or colony, were larger than those of the latter four species which formed their chain or colony and adhered tightly to the substrate. This result may suggest that the latter type diatom competes with each other for light, nutrients, space and so on more severely than the former.
  • 北村 等, 平山 和次
    1984 年 50 巻 6 号 p. 973-977
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Two slide glasses each bearing three to five larvae of Bugula neritina attached had been placed in a one-liter beaker holding 800m/ of filtered seawater. After the daily renewal, adequate volume (10-60m/) of the cultured plankton was supplied into the beaker to make the food suspension at a definite density. The geometric means of the zooid number per colony after ten-day's culture with various food planktons were determined to compare the dietary effect. The salinity and temperature of culture water were maintained from 33.4 to 34.3‰ and from 21 to 23°, respectively.
    Of 24 species examined, Rhodomonas sp. supported the best growth; all young colonies exponentially increased the zooid number, from one to 72 in ten days. The growth was almost equal to that under the best natural conditions. Oxyrrhis marina cultured in the medium enriched with yeast extract was the most suitable food next to Rhodomonas (48 zooids in ten days). When fed on Dunaliella tertiolecta and Prorocentrum obtusidens, the colonies increased the zooid number only to 18 and 12, respectively. Diatoms and armored planktons had no dietary value for the animal.
  • 桂 和彦, 山崎 文雄, 浜田 啓吉, 大石 圭一, 原田 武夫, 新川 智昭
    1984 年 50 巻 6 号 p. 979-984
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The present study made clear the distribution and the frequency of tumorous flatfish and goby in the coastal waters of Hokkaido, Japan. The tumorous fish had the histological characteristic of having X-cells in the epithelial lesions. The species having these tumorous lesions were Kareius bicoloratus, Limanda schrenki, Platichthys stellatus, Cleisthenes pinetorum herzensteini, Hippoglossoides dubius, Verasper moseri, Liopetta obscura among flatfish, and one species of goby Acanthogobius flavimanus.
    The tumorous flatfish were distributed in the coastal waters of the Japan Sea side from Teshio to Soya Bay, and on the side of the island facing the Okhotsk Sea, teh Nemuro Strait and the Pacific Ocean, from Kushiro to the city of Nemuro. The tumorous gobies A. flavimanus were collected only at Nanachama Shore, Hakodate Bay. The highest frequency recorded was 40% for P. stellatus from Lake Tofutsu.
    Yearly changes in the frequency of tumorous fish were recognized for L. schrenki from Tokotan on the Nemuro Strait, for K. bicoloratus from Soya Bay, and also for A. flavimanus from Hakodate Bay. Species specificity was recognized in the tumorous infection of flatfishes.
    This tumorous infection may be endemic. The high frequency of these tumorous fishes is not directly related to municipal or industrial discharge, but to muddy bottom caused by stagnted sea water.
  • 加納 碩雄, 野口 玉雄, 丸山 純一, 橋本 周久
    1984 年 50 巻 6 号 p. 985-990
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Ninety-six specimens of the pufferfish Fugu pardalis (“higanfugu”) landed at Tokyo Metropolitan Central Wholesale Market were assayed for toxicity of liver, ovary, bile and digestive canal contents.
    Frequency of toxic sepcimens were 92, 96, 69 and 50%, in terms of liver, ovary, bile and digestive canal contents, respectively. Their highest toxicity scores were 4, 900, 4, 100, 1, 700 and 270 MU/g, respectively; and average toxicity values were 565±93, 590±66, 162±34 and 43±8 MU/g, respectively.
    A significant correlation between the toxicities of liver and bile (r=0.63), and between those of bile and digestive canal contents (r=0.69) was observed.
  • 平石 明, 佐伯 和昭, 堀江 進
    1984 年 50 巻 6 号 p. 991-997
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Total coliform (TC) and fecal coliform (FC) organisms were enumerated and isolated from water samples from several stations on the Tamagawas River and its tributaries in Tokyo. The level of these indicator bacteria and biochemical oxygen demand(BOD) markedly increased from the upper streams towards the lower reaches of this river system. There were statistically significant positive correlations (p<0.01) among TCs, FCs, and BOD. Also the FC/TC ratios were highly correlated with BOD (p<0.01). The proportion of thermotolearant coliforms and Escherichia coli constituting the TC population increased with increasing BOD levels. whereas the relative concentration of E. coli to the FC population decreased with increasing BOD levels. These results suggest that the population densities of FCs or thermotolearnt coliforms in the river environment are directly proportional to the degree of sewage and human waste pollution, which is reflected by BOD levels, but their occurrence in polluted river and esturay waters is due in part to regrowth of some FC members such as Klebsiella pneumoniae.
  • 鎌谷 明善, 天野 誠
    1984 年 50 巻 6 号 p. 999-1003
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The fecal pellets collected from water-sediment interface by pumping were identified microscopically to be composed of a mixture of phytoplankton detritus and minerals, and then submitted for a study of the regeneration rates of phosphate and silica under laboratory conditions. Under aerobic proceeding, inorganic phsophate regenerated from the detritus was sorbed exclusively on the pellets until the sorption capacity was aturated by phosphate; while with development of anaerobic conditions the phosphate sorption capacity was reduced and the desorption of inorganic phsphate started instantaneously. The fecal pellets thus appeared to act as an important phosphate buffer. The aerobic regeneration rate of phosphate (Kp, day-1) can be predicted as a function of temperature (t°C); log Kp=2.61×10-2t-2.05, and the dissolution rate of biogenic silica in the pellets (Ksi, day-1); log ksi=4.53×10-2t-3.15. The study strongly supports the assumption that phosphate and silica regenerated from the fecal pellets supply a significant fraction of nutrients required by primary producers in the water column during summer months when anoxic conditions are spreading in the water-sediment interfce.
  • 進藤 くみ子, 土屋 隆英, 松本 重一郎
    1984 年 50 巻 6 号 p. 1005-1008
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    In order to find suitable conditions for the fixation of fish muscles for electron microscopy, effect of the fixative concentration to the musculature of the freshwater and saltwater fish was examined. Carp and horse mackerel were used as the sample of the freshwate and saltwater fish, respectively. Muscle samples were prefixed in 3% glutaraldehyde-sodium cacodylate buffer (pH 7.2). Following the postfixation in 1% OSO4-sodium cacodylate buffer, muscles were dehydraed in ethanol and embedded in TAAB 812. The molarity of sodium cacodylate buffer was set to 0.05M, 0.1M or 0.2M for the freshwater fish, and to 0.1M, 0.2M or 0.3M for the saltwater fish, in both prefixation and postfixation Muscle samples were block-stained in 1% uranyl acette solution ater postfixation. Ultrathin sections were examined under electron microscope. The results show that the most suitable concentration of the fixatives is 0.1M and 0.2M for the freshwater and for the saltwater fish muscles, respectively. Use of the fixatives lower than the above strengths induced expansion of the mitochondria or the sarcoplasmic reticulum and broadening of the myofibrils in both fresh and salt water fish muscles. On the other hand, use of the fixatives stronger than the above induced deformation of the mitochondria, and obscuration of the filament structures.
  • 佐藤 守, 吉中 禮二, 岡本 一磨, 池田 静徳
    1984 年 50 巻 6 号 p. 1009-1013
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Distribution and some properties of prolyl hydroxylase (EC 1.14.11.2) which catalyzes the hydroxylation of proline in a polypeptide precursor of collagen were studied in rainbow trout.
    The enzyme activity was found to be present in various tissues of rainbow trout. The specific activity was highest in the centrum. Relativity high specific activity was also found in the swimblakdder, skin, and gill. The enzyme was located the microsomal and soluble fractions of the liver. The enzyme from th liver required α-ketoglutaric acid, ascorbic acid and ferrous ion for activity. The optimum temperatrue for the activity of the enzyme from the rainbow trout liver was around 20“C, whereas that of the enzyme from the carp hepatopancreas was around 25”C. The differece in the optimum temperature for the activity of the enzyme of these two fishes might be related to their optimum living temperatures.
  • 渡辺 武, 伊藤 暁, 北島 力, 藤田 矢郎
    1984 年 50 巻 6 号 p. 1015-1022
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    A relatively long-term feeding experiment was conducted in order to compare the nutritional quality as a protein source between white fish meal and cuttlefish meal and to investigate an optimum protein level in diet of red sea bream broodstock for their reproduction. The percentage of buoyant eggs, total hatch and normal larvae obtained obtained was not so markedly different among eggs produced by the broodstock receiving respectively different diets with different protein levels except for those obtained from the broodstock given the diet containing cuttlefish meal as a protein source. In the broodstock fed on the control diet containing white fish meal (CP 45%) the average number of eggs produced during the experimental period was 205.1×104 per fish, and 61.8% of these eggs were found to be buoyant. Wherase in the eggs produced by the broodstock receiving the cuttlefish meal diet the proportion of buoyant eggs was highest (83.5%) ammong the expeimental groups. In addition, the percentage of normal larvae available as fish seed was markedly improved from 24.2% to 79.5% by replacing white fish meal with cuttlefish meal as a protein source. Thus it has been confirmed again that cuttlefish meal is superior to white fish meal as a protein source in diets for red sea bream broodstock. An optimum protein level was not clearly defined in this expeiment, but was estimated to be around 45% judging from the present and previous results.
  • 渡辺 武, 伊藤 暁, 村上 あかね, 塚島 康生, 北島 力, 藤田 矢郎
    1984 年 50 巻 6 号 p. 1023-1028
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    This study was conducted to examine the effect of nutritional quality of diets given to broodstock on the ve ?? ge of spawning on reproduction of red sea bream, and to clarify whether or not egg quality is improved by enhancing incorpration of pigments from diets. The percentage of buoyant eggs was found to be improved by supplementing pigments such as β-carotene and canthaxanthin or astaxanthin to the diets given to broodstock on the eve of spawning. Feeding frozen raw krill shortly before spawning also resulted in marked improvement in both the tatal eggs produced and the percentage of buoyant eggs. On the other hand, in the group fed on the corn oil diet on the verge of spawning the proportion of buoyant eggs was markedly reduced from the original value by replacement of cuttlefish liver oil with corn oil. The rate of hatching was not improved by addition of pigments, but abnormality in number and position of oil globules was reduced and consequently the total number of normal larvae obtained became higher than in the original broodstock. These values were also effectively improved by feeding on frozen raw krill and the percentage of normal larvae obtained from buoyant eggs was elevated from the original value of 51.6% to 91.2%, whereas replacement of cuttlefish liver oil with corn oil in the given to broodstock even in a short period during spawning resulted in lowering of these values.
  • 赤羽 孝之, 千原 聡, 吉田 豊, 土屋 隆英, 野口 敏, 大神 弘司, 松本 重一郎
    1984 年 50 巻 6 号 p. 1029-1033
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    This study was undertaken to indentify the key protein (s) which bring about the gel properties of kamaboko and other cooked gel products made from muscular proteins. Mixtures of rabbit actin and myosin of various ratios were prepared. To each mixture was added 2.5% NaCL and cooked in three differet ways: Method I, cooked at 85°C for 30min; Method II, held at 30°C for 30min and cookde as I; Method III, held at 4°C for 24h and cooded as I. Gels optained were submitted to the jelly strength measurement and the differential scanning calorimetry (DSC). The jelly strength values of the gels cooked by Method I increased with increasing myosin content. This indicated that myosin is the essential factor for high jelly strength. Effects of setting treatments of Method III in enhancing the jelly strength were more evidently manifested in the gels with higher myosin content. No effect was found with Method II. The R105 values of DSC which represent the water holding capacity of the gels paralleled the above jelly trength values, indicating that the highly hydrative property of myosin is an essential factor for the high jelly strength. Co-presence of actin and myosin was found essential to bestowing the precooking material with pastiness and consistency with pastiness and consistency which enable the precooking handling like molding or shape giving.
  • 奥谷 康一
    1984 年 50 巻 6 号 p. 1035-1037
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    Antitumor activity of the polysaccharide prepaation from marine Vibrio sp. and its effects on immune responses were studied by using mice.
    Antitumor activities of the material were examined agained against P 388 leukemia and Sarcoma-180 solid tumor cells. The saccharide showed a weak antitumor activity against both tumors. In the test of lymphocyte responsiveness to phytohemagglutinin (PHA), the test sample reduced the immunological response to PHA. The cellular immune rsponses were measured by the delayed hypersensitivity reaction to sheep red blood cells using footpad assay. The preparation suppressed the production of antibody against sheep erythrocytes in mice at high doses. The appearance of antibody forming cells after the injection of sheep red blood cell antigen was studied by the plaque technique. The number of plaque forming cells without treatment of this material is about 1×104 cells per spleen, which increased to about 5×104 by treatment of this materia.
  • 滝井 健二, 竹田 正彦, 中尾 嘉弘
    1984 年 50 巻 6 号 p. 1039-1043
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The effects of supplementing formulated feeds with feeding stimulants (four amino acids plus 5'-UMP) on the feeding activity and growth of young eel Anguilla japonica ere studied by means of two-choice preference tests and by separate feeding tests with the unflavoured feed and the flavoured feed.
    Elver showed a marked prference for the flavoured feed over the unflavoured feed. While increasing the rearing temperature from 27°C to 28°C resulted in lowered feeding activity of young eel, the flavoured dietary group maintained a relatively high weight gain and feed efficiency at 28°C. Also, the weight gain and feed efficiency of th eflavoured dietay group reared at 24°C were significantly higher than those of the control groups at 24°C or 27°C. Groups of young el fed on the flavoured feed for 40 days showed a preference for the flavoured feed when given simultaneous access to flavoured and unflavoured feeds.
    The results obtained are of some relevance to the development of palatable feed for elver and of saving energy in the warm culture of eel.
  • 磯 直道, 水野 治夫, 斎藤 隆英, 大関 文照, 栗原 信夫
    1984 年 50 巻 6 号 p. 1045-1049
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The change of the rheological properties of surimi which accompanied heat-rocessing was examined by stress-relaxation experiment. Samples were made from the frozen surimi of Alaska pollack. The instantaneous compression modulus showed the measuring temperature dependence, and the results could be analyzed by the theory of rubber elasticity. All the samples except those produced by heating at 40°C showed the thermo-rheological simplicity. Although the elastic modulus of all the samples except those of 0% NaCl generally increased with the heating temperature, the viscosity was almost independent of the heating temperature.
  • 菅原 庸, 林 孝市郎, 木村 俊夫, 豊田 弘, 松岡 明彦, 山中 重和
    1984 年 50 巻 6 号 p. 1051-1055
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The effect of NaCl on the production of bacteriolytic enzyme by a bacterium isolated from coastal waters was investigated using polypepton-yeast extract medium.
    The production of lytic enzyme was not enhanced by the combination of NaCl and KCl, while Nacl and KCl is respectively effective for the enzyme production. Strain V 37 cells grown in the medium without added NaCl for 3-6 hours produced extracellular lytic enzyme immediately after Nacl was added into the growth medium. However, the stationary-phase cells could not produce lytic enzyme by the addition of Nacl. Strain V 37 cells grown in the medium containing NaCl could not produce extracellular lytic enzyme considerably after transferred into the medium without added NaCl. Strain V 37 seemed to require NaCl for the enzyme production during the active growth.
  • 菅原 庸, 林 孝市郎, 木村 俊夫, 山中 重和, 丹羽 啓之, 谷岡 信良
    1984 年 50 巻 6 号 p. 1057-1060
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The suppression of the production of lytic enzyme was investigated using alcohols as membrane-modifying agents.
    At the concentrations which growth was not suppressed, the production of lytic enzyme by strain V 37 was inhibited by n-alcohols having a range of 8 to 14 carbon atoms, phenethyl alcohol and benzyl alcohol, respectively.
    The formation of extracellular lytic enzyme by the cells from the lag phase to the early logarithmic phase of growth was most susceptible to membrane-modifying agents such as octanol and phenethyl alcohol.
  • 藤井 建夫, 酒井 久夫
    1984 年 50 巻 6 号 p. 1061-1066
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The chemical and microbiological analyses of shotturu (Japanese fish sauce) produced in Akita district were carried out. The composition of shotturu was as follows: pH 6.0, NaCl 27.5-27.8%, total mitrogen 644-735mg/100ml, volatile basic nitrogen 74.4-82.0mg/100ml, trimethylamine 8.4-12.4mg-N/100ml and acetic acid 19.3mg/100ml. Both aerobic and anaerobic viable cell counts in 2.5% NaCl-and 20% NaCl-medium were between the range of 2.3×103-3.6×105/ml. The dominant microbial flora were Vibrionaceae and Bacillus in 2.5% NaCl-medium, and the members of genus Halobacterium, Bacillus and unidentified cocci in 20% NaCl-medium.
  • 藤井 建夫, 酒井 久夫
    1984 年 50 巻 6 号 p. 1067-1070
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    The chemical and microbiological analyses of putrid shotturu (fish sauce) were carried out in order to compare them with the normal products investigated in the preceeding article. The results were as follows: pH 6.2-6.3, NaCl 26.5-27.0%, total nitrogen 658-693mg/100ml, volatile basic nitrogen 89.1-95.2/100ml, trimethylamine 12.2-32.1mg-N/100ml. The volatile acids showed high values of 92.9mg/100ml for acetic acid and 25.8mg/100ml for n-butyric acid. The aerobic and anaerobic viable cell counts in 2.5% NaCl-and 20% NaCl-medium increased up to 5.8×106-2.8×107/ml, though the counts drastically changed during storage. The dominant microflora (aerobes) of putrid shotturu in 2.5% NaCl-medium were the bacteria of genus Streptococcus, and the one in 20% NaCl-medium were the members of Halobacterium, Micrococcus, Bacillus and unidentified cocci.
  • 松野 隆男, 眞岡 孝至, 片桐 清, 小森 忠昭
    1984 年 50 巻 6 号 p. 1071-1075
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    β-Carotene (21.1%), isorenieratene (5.7%), isorenieracistene (23.1%), trikentriorhodin (9.2%) and a new carotenoid (13.4%) were isolated from the sea sponge, Suberites sericeus.
    The structure of the new carotenoid has been established to be 7, 7'-dicis-φ, φ-carotene by chemical and physical methods including 1H-NMR, IR, and mass spectra.
    It is interesting from biosynthetic point of view that S. sericeus possesses relatively high contents of 7-cis carotenoids which is rare in nature.
  • 冨岡 和子, 遠藤 金次
    1984 年 50 巻 6 号 p. 1077-1081
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
    An enzyme which catalyzes the hydrolysis of 5'-IMP to inosine and inorganic phosphate was solubilized from skeletal muscle of carp Cyprinus carpio with a detergent, Emulgen 109P, and was purified by ammonium sulfate fractionation, two steps of affinity chromatography on Concanavalin A-Sepharose and 5'-AMP Sepharose 4B. The purified enzyme was homogeneous on disc gel electrophoresis. An apparent molecular weight of the purified enzyme was estimated to be 24×104 by gel filtration with Sephadex G-200 in the presence of the detergent. The enzyme was found to be a 5'-nucleotidase (EC 3. 1. 3. 5) on the basis of its substrate specificity.
  • 柳沢 忠, 橋本 周久
    1984 年 50 巻 6 号 p. 1083
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
  • 嵯峨 直恒, 阪井 與志雄
    1984 年 50 巻 6 号 p. 1085
    発行日: 1984/06/25
    公開日: 2008/02/29
    ジャーナル フリー
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