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Volume 50, Issue 9
Displaying 1-28 of 28 articles from this issue
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Jiro KOYAMA, Toshifumi ADACHI, Yayoi IKEDA1984 Volume 50 Issue 9 Pages 1469-1473
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe activities of alkaline phosphatase (Al-Pase), acid phosphatase (Acid Pase) and glutamic oxaloacetic transaminase (GOT) were measured using liver, muscle and gill of fishes collected in the Watarase River and the Akiyama River, for examining the usefulness as water quality indices.
There has been little effect of human pollution in St. 1 and the water quality of it was the best among the three stations. St. 2 has been polluted with household sewage and industrial waste water, while St. 3 has been polluted with mine drainage and household sewage. Heavy metal (Cu, Zn, Cd and Pb) contents in viscera of fishes collected at St. 3 were the highest among the three stations. When the tissue enzyme activities of Tribolodon hakonensis collected at St. 2 and St. 3 were compared with those at St. 1, activities of Al-Pase and GOT in muscle and Al-Pase and Acid Pase in gill increased markedly. At St. 2 and St. 3, relativel;y low activities of Al-Pase and Acid Pase in gill may be partly due to high concentration of synthetic detergent because of relatively high concentration of methylene blue active substance. On the other hand, those activities of Zacco platypus were not different among the three stations. GOT activity of both species of fishes seems to depend on water temperature.
When the fish species is properly selected, it may be possible to use the activities of tissue enzymes of fish as water quality indices.View full abstractDownload PDF (319K) -
Masao TAKAHASHI, Shiro MURACHI, Shinpei MORIWAKI, Satomi OGAWA1984 Volume 50 Issue 9 Pages 1475-1480
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSSix bluegill sunfish were trained in a free operant situation during 90- to 200-day periods including 55 to 86 30-min training sessions to obtain food by thrusting up a bobber (bobber upthrust response: BUR) and thus activating an automatic feeder. Individual variation in the level of the BUR was great, varying between 22.9 and 52.0 responses/session on an average. Enhancement of the level of the BUR with increased numbers of trainings over the experimental period was proved statistically for only 2 fish out of the 6. Within a session, decreasing trends in the level of the BUR were observed for all specimens. The sequential mean levels of the BUR obtained from consecutive 2.5-min blocks of each session fit a negative exponential function (y=aebx; y is the mean levels of BUR for xth block, a and b are constant, b<0) well. In contrast to the great variation in the level of the BUR between specimens, the rates of attenuation, expressed by (1-eb), conform rather well, ranging between 0.06 and 0.13.View full abstractDownload PDF (429K) -
Akinori HINO, Reijiro HIRANO1984 Volume 50 Issue 9 Pages 1481-1485
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSBrachionus plicatilis was cultured individually and successively under four (15, 20, 25 and 30°C) temperature conditions, and the frequency of mictic female production was examined. In order to avoid the density effect derived from accumulation of metabolites, culture medium was renewed at regular intervals proportionally to the metabolic rate which was specific to each temperature.
The hereditary differences in bisexual reproductivity existiong among strains could be negligible through experimenting on identical strain. A hundred substrains were made from the same stock, and cultured for 7-8 generations, picking up five offsprings in one generation-one strain. Data from the initial two generations were rejected, because a temporary lowering of mictic female production was observed just after the transference into new environmental condition.
Bisexual reproduction rate increases in lower temperature below 25°C. There is no significant diferrence between 25 and 30°C groups.View full abstractDownload PDF (292K) -
Kiyoshi AMAGAI, Rihei KAWASHIMA1984 Volume 50 Issue 9 Pages 1487-1494
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDynamical relation between ship's motions and warp tension while towing a trawl net was identified by the use of statistical method to be the multivariate AR model.
Using this system identification method, the various towing system parameters were obtained by analysing the records of measuring ship's motions and warp tension during the fishing operations in several sea conditions.
Based on these analyses, using the obtained system models, the future values of the time series were forecasted and the validity of the forecasting of warp tension time series was discussed.View full abstractDownload PDF (504K) -
Takayoshi UEDA, Yoshio OJIMA1984 Volume 50 Issue 9 Pages 1495-1498
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe precise constitution of chromosomes from the young kokanee salmon Oncorhynchus nerka was stably obtained by means of the short-term (for 4-7 days) fin cultures. All ten females have 58 diploid chromosomes set consisting of 46 meta- (M) and submetacentrics (SM), and 12 acrocentrics (A), while all ten males have 57 chromosomes (2n) consisting of 47M and SM, and 10 A. The male always has one less chromosome than the female but both sexes have the same NF (nombre fondamental), 104. These results strongly indicate that the male has the X1X2-Y, while the female has the X1X1-X2X2 in sex chromosome complexes. This fact would make it possible to select males and females at the young stage of the kokanee salmon without sacrifice of the materials.View full abstractDownload PDF (1290K) -
Takayoshi UEDA, Yoshio OJIMA1984 Volume 50 Issue 9 Pages 1499-1504
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwo strains (I and II) of the rainbow trout Salmo gairdneri have been breeding at the Nikko Branch of the National Research Institule of Aquaculture, Fishery Agency (Tochigi Prefecture). The precise constitution of chromosomes from the young fishes was stably obtained by means of the short-term (for 4-7 days) fin cultures. All the fishes of strain-I have 60 chromosomes in diploid, consisting of 44 meta- (M) and submetacentrics (SM), 2 subtelocentrics (ST), and 14 acrocentrics (A). In the fishes of strain-II, the variaions were observed on the chromosome numbers. These variations would be induced by the Robertsonian-type translocations in relation to the interspecific differentiation. And the male always has one less ST than the female. This result indicates that the male has the X-Y, while the female has the X-X in sex chromosome complexes. This fact would make it possible to select males and females at the young stage of the rainbow trout in strain-I without sacrifice of the materials.View full abstractDownload PDF (4588K) -
Toshitaka NISHIJIMA, Yoshihiko HATA1984 Volume 50 Issue 9 Pages 1505-1510
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPhysiological characteristics on the growth of Heterosigma akashiwo HADA (Raphidophyceae), a red tide phytoplankton, were examined with special reference to B group vitamin requirements in the axenic clonal culture.
The alga was wide-range euryhaline, and preferred relatively high concentration of nutrients. It was found that only vitamin B12 was essential for the growth, and that growth rates and final cell yields were dependent on the vitamin B12 concentrations in the media. Vitamin B12 halfsaturation constants were very small: 0.37-0.60ng/l. However, it needed the higher concentration of 20ng/l to maintain maximum growth yields. Maximum specific growth rates were 0.62-0.82 day-1.
The minimum cell quota of vitamin B12 was 8.9×10-5pg/cell or 24.2 molecules/μm3 of the cell volume. Cells grown in the vitamin B12-rich media were able to accumulate it up to 50-fold as much as the minimum quota.
These results suggest that vitamin B12 in seawater may be one of the most principal factors controlling the growth of H. akashiwo, and consequently the development of its population to a red tide.View full abstractDownload PDF (422K) -
Charles S. MANOOCH, Diane L. MASON, Russell S. NELSON1984 Volume 50 Issue 9 Pages 1511-1525
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA total of 2, 632 dolphin Coryphaena hippurus, 250 to 1, 530 millimeters fork length (FL), were captured by hook and line off the southeastern United States and from the Gulf of Mexico in 1980 and 1981. Eighty-four percent (2, 219) of the stomachs contained ingested materials consisting of 13, 383 individual items, displacing 57, 648 milliliters, and representing 248 different categories. Fishes occurred in 77.6% of the stomachs, invertebrates in 27.5% and miscellaneous items (Sargassum, tar balls, plastics, etc.) in 50.6%. Much of the material indicated that dolphin frequently feed at the surface and ingest fishes, crustaceans, insects, plants, and inorganic items that are associated with floating Sargassum. Index of relative importance (IRI) revealed unidentified fish, balistids, crustaceans. carangids, exocoetids, teuthidiEs (squids), syngnathids, coryphaenids, stomatopods, and diodontids as the 10 most important foods in the diet. Sargassum, which occurred in 48.6% of the stomachs, was considered to be consumed incidental to normal foods. The diets differed with size of dolphin (8 size classes), area of collection (10 areas), and season (4 seasons). The ascaridoid nematode Hysterothylacium pelagicum sp. n. and an unidentified digenetic trematode were found in the digestive tracs. Nematodes were far more numerous and occurred more frequently than did trematodes. Infestation rate seemed to be more associated with size of dolphim than with season or area of collection.View full abstractDownload PDF (1197K) -
Masahiro MUNEKIYO, Akihiko KUWAHARA1984 Volume 50 Issue 9 Pages 1527-1533
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA series of exploratory fishing expeditions utilizing gill nets was carried out to determine the spawning habits of the ribbon fish Trichiurus lepturus with regard to the bathymetric distribution, in the western Wakasa Bay from June to July, 1983. The maturation of both sexes and the sexratio of ribbon fish capturd in the offshore waters (depths of 100-160m) were comapred with catches from the inshore waters (depths of 40-60m) by commercial longline fishing.
The sex ratio of the ribbon fish in the inshore waters highly skewed to female in various stages of maturity. On the other hand, the sex ratio of the ribbon fish in the offshore waters skewed to male with high sexual maturity. Females in the offshore waters also were much more mature than those from the inshore waters. It was, therefore, determined that both sexes of ribbon fish were separately distributed just before mating, namely males in the offshore waters and females in the inshore waters, respectively, and highly matured females shifted out from the inshore waters, so the spawning ground was formed in the offshore waters. According to EMLEN and ORING's classification of mating systems, spawning habits of the ribbon fish were characterized to be “Lekmating systems”.View full abstractDownload PDF (1240K) -
Toyoji KANEKO, Isao HANYU, Keiji HIROSE1984 Volume 50 Issue 9 Pages 1535-1540
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe tidepool-living goby Chasmichthys dolichognathus was collected around Arasaki in Kanagawa Prefecture at intervals of about a month from March 1980 to July 1982. Seasonal changes in the GSI and the gonadal histology indicated that their spawning season extended from February to May. During this period, females with empty follicles in their ovaries were often observed. In female fish, the GSI increased rapidly in December with concomitant accumulation of yolk in the oocytes. In June it decreased suddenly, as the oocytes underwent regression. The seasonal change of GSI in males was similar to that in females, although testicular lobules started to develop in October and sperms appeared in December. The rapid changes in the GSI suggest that water temperature plays an important role in both initiation and termination of their spwaning season.
Hepatic cells of females showed a strong affinity to hematoxyline from November to May, while the cells were weakly stained in the other period. Those of males did not exhibit such an apparent seasonal change. In mature fish, a major part of proximal pars distalis in the pituitary gland consisted of gonadotrophs which had showed a strong affinity to aldehyde-thionine. Those of immature fish, however, were less in number and weakly stained.View full abstractDownload PDF (2884K) -
Katsuhiko HARADA, Sen MARUYAMA, Kazumi NAKANO1984 Volume 50 Issue 9 Pages 1541-1544
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe attraction indexes to abalone of proteins, lipids, amino acids, volatile bases and betaines which were fractionated from the brown alga Ishige okamurai were higher than that of dummy as control. The indexes of the four constituents except the betaines were approximately equal to that of the whole extract and higher at different concentrations than that of dummy. In this context, it was estimated that the feeding attractants for abalone are the proteins, the lipids, the amino acids and the volatile bases.
Among a variety of two- and three-constituent combinations, the attraction indexes of combinations of the proteins-lipids and the proteins-lipids-amino acids or betanines were appreciably more effective than those of its single constituent and/or two-constituent combinations. These results reveal that the attraction is reinforced collaborately by two-and three-constituent combinations.View full abstractDownload PDF (285K) -
Hiroshi NIKI, Tôru DOI, Seiichiro IGARASHI1984 Volume 50 Issue 9 Pages 1545-1550
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe high viscosity of fish meat sol is one of the most difficult barriers on the development of the active fish protein powder (AFPP). The method which reduce the viscosity of fish meat sol and retain the kamaboko-forming property of fish meat should be developed.
The effects of adding Ca2+, Ca2+ and poly anion, water soluble protein and carbonic acid to fish meat sol were examined.
These four methods reduced the viscosity of fish meat sol sufficiently. But only the fourth method which added carbonic acid to fish meat sol, lowered the pH of sol and reduced the viscosity of sol could make the AFPP that has the highest kamaboko-forming property. It is the feature of this method that carbonic acid added to fish meat sol was removed from fish meat quickly through spray-dring and was not retained in the AFPP. Therefore, pH of the AFPP became neutral and the denaturation of fish meat protein (actomyosin) was little.View full abstractDownload PDF (481K) -
Kiyoko SAEKI, Hiroshi KUMAGAI1984 Volume 50 Issue 9 Pages 1551-1554
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThis investigation was carried out in order to elucidate the characteristic of cultured fishes compared with wild fishes. The contents of chemical components such as moisture, protein, lipid, and ash were determined for the muscles of ten kinds of wild and cultured fishes. The cultured fishes were taken from the Senzaki culture farm in Yamaguchi Prefecture and the wild fishes were caught in coastal waters of Hagi near Senzaki in March, 1978.
The fishes studied have been found to be classified into three groups in terms of the relative contents of the nutritive components of the cultured fishes to contents of the wild fishes. In the first group which include rock fish, common nibbler, oval filefish, and fringed filefish, the lipid content is richer, while the moistrue content is poorer in the cultured fishes. In the second group to which horse mackerel, black sea bream, and scorpionfish belong, the lipid content is richer and the moisture content is not significantly poorer than in the cultured fishes. For the fishes of the last group: parrot bass, grey mullet, and puffer, there is no appreciable adfference in the contents of all the nutritive components between cultured and wild fishes.
The results elucidated that the cultured fishes are not always richer in the lipid content than the wild fishes, which is not consistent with the current findings that the cultured fishes are richer in the lipid content as compared with the wild fishes.View full abstractDownload PDF (237K) -
Bunji YOSHITOMI, Shin-ichi KAMIYA, Kunihiko KONNO1984 Volume 50 Issue 9 Pages 1555-1559
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe method for isolation of heavy meromyosin (HMM) from squid mantle myosin was developed, and the biological properties of HMM were studied.
1) HMM was prepared by digesting squid myosin with trypsin (1/500, w/w) at 10C° for 10min, followed by addition of trypsin inhibitor. The digest was dialyzed against 0.05M KCl, pH 7.5, and centrifuged at 100, 000×g for 120min. The supernatant was used as squid HMM solution.
2) Squid HMM thus prepared was homogeneous both in ultracentrifugation and in Sephacryl S-300 gel filtration.
3) Ca-, Mg-, and EDTA-ATPases of squid HMM showed essentially the same KCl concentration, pH, and temperature dependencies as those of squed myosin.
4) Mg-ATPase of squid HMM alone was sensitive to Ca as was acto-HMM. Ca-sensitivity of HMM disappeared in the presence of 1-1.3M urea in the reaction medium.View full abstractDownload PDF (2948K) -
Yoshinori MIYAMOTO, Takeshi SHIBATA1984 Volume 50 Issue 9 Pages 1561-1565
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to undertake a more complete survey of the properties of the glyceraldehyde-phosphate dehudrogenase (EC. 1. 2. 1. 12) and to establish the taxonomic relationships among the species from which the eczyme were isolated, antiserum was prepared in rabbit against the enzyme purified from chum salmon muscle. Gel diffusion and enzyme inhibition experiments were carried out with the homologeneous enzymes isolated from other species of salmonid and gadoid fishes.
The data obtained show the following results: (1) Each enzyme isolated from salmonoid fish showed a cross-reaction with the antiserum, indicating the immunological homology among these enzymes; (2) The antiserum cross-reacted weakly with the gadoid enzyme and its concentration was increased by 2-3 fold for the production of quantitative precipitin and enzyme inhibition in the cross-reacting system, indicating the partially structural similarity between the enzyme from the two bony fishes; (3) The antiserum did not cross-react with the rabbit enzyme over a wide concentration range, indicating considerable structural differences between the enzymes from the two organisms.View full abstractDownload PDF (1155K) -
Toshiaki OHSHIMA, Shum WADA, Chiaki KOIZUMI1984 Volume 50 Issue 9 Pages 1567-1572
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe effect of the free fatty acid (FFA) released from phospholipids by the action of lipolytic enzymes on protein denaturation of fish flesh during frozen storage was studied. The FFA content and the salt soluble protein extractability of commercially available frozen “surimi” were determined. The results obtained suggest that there may be a definite negative interrelation between the FFA content and the salt soluble protein extractability.
Minced cod flesh was washed thoroughly with cold water, mixed with phospholipase A2 specimen, and stored at -16°C for 24 weeks. The FFA content incerased rapidly accompanied with marked decreases of polar lipids due to added phospholipase A2 during storage for first one week. The content of salt soluble protein increased during storage for up to 1-2 weeks and then decreased gradually to the minimum amount after storage for 6 weeks. The length of storage period up to the time when the content of salt soluble protein reached the minimum amount was shorter in the test-sample than in the control-sample without added phospholipase A2.
From these results, it is presumed that the FFA released from phospholipids by the action of added phospholipase A2 induces the reduction of salt soluble protein extractability, though some oxidation products of lipids may contribute to the reduction of salt soluble protein extractability to some extent.View full abstractDownload PDF (427K) -
Toshio SAITO, Junichi MARUYAMA, Sekio KANOH, Joong-Kyun JEON, Tamao NO ...1984 Volume 50 Issue 9 Pages 1573-1575
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSEighty-five cultured specimens of a pufferfish Fugu rubripes rubripes were collected from several localities and assayed for toxicity as tetrodotoxin (TTX), as a part of a study on the mechanism involved in the toxification of pufferfish. No toxicity was detected regardless to the locality of collection, the age and tissue of specimen, etc.
Attempts were made to estimate the resistibility against TTX of those cultured pufferfish. Results showed that they were endowed with high resistibility: the minimal lethal dose (i. p.) was 300-500 MU/20g body weight.
All these results are supposed to favor the view that the pufferfish is toxified exogenously, i. e., by a food chain.View full abstractDownload PDF (229K) -
Kazuki HARADA, Aritsune UCHIDA, Hajime KADOTA1984 Volume 50 Issue 9 Pages 1577-1582
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe cells of Deinococcus radiodurans were not killed when they were independently treated by gamma-irradiation at 300 krad or heating at 55°C for 8min. However, some synergistic effects of gamma-irradiation and mild heating on the survival of the bacterium were found when the cells were treated by the combination of these different agents, and this synergistic killing effect was irrespective of the treatment procedure.
The DNA of the cells which were heated at 55°C for 8min immediately after gamma-irradiation at 300 krad was more disintegrated to smaller molecular weight than that of the cells which were simply heated or irradiated. The DNA breaks caused by the combined treatment of gamma-irradiation and mild heating were not repaired after even 480min incubation in growing medium.
Therefore, it is thought that the repair mechanisms of radiation-induced DNA injury and mild-heating-induced DNA injury are probably common.View full abstractDownload PDF (451K) -
Takao MATSUNO, Kiyoshi KATAGIRI, Tkakshi MAOKA, Tadaaki KOMORI1984 Volume 50 Issue 9 Pages 1583-1588
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe carotenoids from the reddish orange coloured muscle tissue of the spindle shell Fusinus perplexus were investigated. (3S)-Phoenicoxanthin (28%), 4, 4'-dihydroxypirardixanthin (18%), and canthaxanthin (14%) were identified as major carotenoids. Furthermore, β-carotene (2%), echinenone (6%), 4'-hydroxyechinenone (5%), lutein (0.5%), zeaxanthin (4%), diatoxanthin (0.5%), alloxanthin (4%), mytiloxanthin (trace), (3S, 3'S)-astaxanthin (4%), and fritschiellaxanthin (6%) were also identified.
4, 4'-Dihydroxypirardixanthin was first isolated as crystals and the proposed structure was confirmed by chemical and spectral data.View full abstractDownload PDF (312K) -
Takao MATSUNO, Takashi MAOKA, Masaaki KATSUYAMA, Masahiro OOKUBO, Kiyo ...1984 Volume 50 Issue 9 Pages 1589-1592
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAstaxanthin fraction obtained from aquatic animals was separated into its optical isomers, (3S, 3'S)-astaxanthin (1), (3S, 3'R; meso)-astaxanthin (2), and (3R, 3'R)-astaxanthin (3) in the free forms by HPLC on optical resolution column.
The occurrence of enantiomeric and meso-astaxanthin in the thirty-seven species of aquatic animals was reported.View full abstractDownload PDF (190K) -
Tateo FUJII, Hisao SAKAI1984 Volume 50 Issue 9 Pages 1593-1597
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to confirm the microorganisms causing the spoilage of fish sauce shotturu, several strains of bacteria such as Halobacterium, Halococcus, Vibrionaceae, Streptococcus and unidentified coccus isolated from shotturu as the dominant groups were inoculated into sterilized shotturu and changes in viable cell counts, the amounts of volatile basic nitrogen, trimenthylamine and volatile odour components as indices of spoilage during storage at 30°C were examined. It was suggested that bacteria belonging to the genus Halobacterium were the most responsible for the spoilage and the other groups of bacteria like Streptococcus having the ability to grow in hignh salt medium were supplementally concerning it.View full abstractDownload PDF (294K) -
Tateo FUJII, Hisao SAKAI1984 Volume 50 Issue 9 Pages 1599-1604
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSChanges in bacterial flora during manufacturing process of fish sauce shottutu were studied. Viable cell counts on 2.5% NaC1-medium in the samples obtained during fermentation (sample C1), after boiling (sample C2), and afer filtration with sea sand (sample C3) were 5.5×105, 7.0×101, and 8.5×104/ml, respectively. Those on 20% NaC1-medium were 9.0×104, 4.1×102, and 3.4×106/ml, respectively. The dominant microbial flora were the bacteria belonging to genus Micrococcus (on 2.5%NaC1-medium), Micrococcus and Corynebacterium (on 20% NaC1-medium) in sample C1, Streptococcus, unidentified coccus (on 2.5% NaC1-medium) and Bacillus (on 20% NaC1-medium) in sample C2, and Streptococcus (on 2.5% NaC1-medium), Vibrionaceae and Bacillus (on 20% NcC1-medium) in sample C3. Viable cell counts in the sea sand used for filtration were 104-105/g. Microbial flora on 20% NaC1-medium were Halococcus and actinomycetes in the sand before use (sample D1), and Vibrionaceae and Enterobacteriaceae in the sand after use (sample D2), respectively. Halobacterium, which is mainly concerned with the putrefaction of shotturu, was detected both in the sample during fermentation (C1) and sea sand (D1, D2), though not predominant.View full abstractDownload PDF (382K) -
Kenji HARA, Hiraki ARANO, Tadasjo ISHIHARA1984 Volume 50 Issue 9 Pages 1605-1609
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAlkaline proteases were isolated from the crude extract of the rotifer Brachionus plicatilis by the combination of gel filtration and ion-exchange chromatogaphy. Two-main active fractions, designated as F-I and F-II, were eluted on the second DEAE-cellulose column, and F-I was finally purified about 190-fold and F-II about 920-fold. F-II was homogeneous as judged from polyacrylamide disc gel electrophoresis. The molecular weihts of F-I and F-II were estimated to be about 800, 000 and 900, 000, respectively, by Sepharose 6B gel filtration, and their isoelectric points were found to be 9.7 and 5.6, respectively.View full abstractDownload PDF (1028K) -
Kenji HARA, Hiraki ARANO, Tadashi ISHIHARA1984 Volume 50 Issue 9 Pages 1611-1616
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSSome enzymatic properties of alkaline proteases (F-I and F-II) were investigated, Optimum pH of F-I and F-II were found to be 8-9, and maximum activities were obtained by incubating the enzymes at 37°C for 1 h. These enzymes were relatively stable over a range of pH 6-8, but markedly unstable above pH 11 and below pH5.5, and the enzymes were completely ionactivated with HgCI2, DFP, TLCK and STI. The action of F-II on insuline B-chain showed that the substrate specificity was quitely similar to that of trypsin.View full abstractDownload PDF (401K) -
Tasuku WATANABE, Minoru YAMAMOTO1984 Volume 50 Issue 9 Pages 1617
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (90K) -
Mariko NAKAYA, Yoichi YOSHIDA1984 Volume 50 Issue 9 Pages 1619
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (100K) -
Isao YANO, Hideki TANAKA1984 Volume 50 Issue 9 Pages 1621
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (92K) -
Mariko IWASAKI, Rokuro HARADA1984 Volume 50 Issue 9 Pages 1623
Published: September 25, 1984
Released on J-STAGE: February 29, 2008
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