巻号一覧
51 巻, 12 号
選択された号の論文の28件中1～28を表示しています
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稲村 浩樹, 中井 敏博, 室賀 清邦1985 年 51 巻 12 号 p. 1915-1920
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe toxicity of extracellular products (ECP) of Vibrlo anguillarum proved to consist in a protease. The extracellular protease was purified by precipitation with ammonium sulfate and gel filtration, and it showed the following characteristics:
1. The LD ?? values of the purified protease to goldfish and mouse were 1.7 and 1.6μgprotein/g weight, respectively.
2. The molecular weight of the purified protease was estimated to be 36, 000 by SDS-polyacrylamide gel electrophoresis.
3. Three serotypes of V. anguillarum produced a common protease in antigenicity.
4. The optimum pH and temperature for the activity of the enzyme was 7 ?? 8 and 50°C, respectively.
5. The protease was inhibited by ethylenediaminetetraacetic acid (EDTA).抄録全体を表示PDF形式でダウンロード (522K) -
長谷川 英一, 添田 秀男1985 年 51 巻 12 号 p. 1921-1926
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーIn order to consider the mechanism of schooling behavior of fish and that maintenance, the stereo method with two cameras was used.
Bitterling Rhodeus ocellatus, dace Tribolodon hakonensis and mackerel Pneumatophorus japonicas (HouTTUYN) were used in this experiment.
The results obtained are as follows: 1) Each individual of the school interacts with each other individual so that its swimming speed fluctuates with time. 2) Each individual of the school constantly supports the distance and orientation of the others. 3) Orderly movement of school is produced when each swimming speed remains steady, and the concentration of the school results in the disorder of that behavior. 4) When the school is turning, each individual regulates its swimming speed according to its position in the school.抄録全体を表示PDF形式でダウンロード (407K) -
嶋村 哲哉, 添田 秀男1985 年 51 巻 12 号 p. 1927-1931
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThis paper dealt with the relation between the fishing effect and soaking time of cuttlefish gillnet.
The results were as follows:
1) The catch of one unit gear (one tan), as soaking time of gear increased, generally changed in 3 steps, this is, increase, decrease and notable increase. But the trend of step varied with fishing ground and/or fishing season.
2) Within the soaking time, 300-1100 minutes, the correlation coefficient between CPUE and soaking time indicated a negative figure, the fishing efficiency decreaced as soaking time of grar increased. Regression coefficient varied with fishing ground and/or fishing season.抄録全体を表示PDF形式でダウンロード (218K) -
川崎 健, 佐藤 一, 武智 文子1985 年 51 巻 12 号 p. 1933-1938
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーIn order to establish a method to estimate population fertility through the survey of commercially caught dab, research on maturation and spawning experiment through rearing fish were carried out. In the course of the former research age, growth by sexes, spawning season, regression of fecundity on body length, proportion of females participating in spawning, etc. were made clear. Through the latter experiment the pattern of maturation and oviposition and the relation between fecundity and fertility were disclosed. As a result, it was recognized that the type of maturation and oviposition of the dab is the bimode-split-batch spawning and the fecundity approximately equals the fertility. Considering the results together, a method to estimate the population fertility is proposed.抄録全体を表示PDF形式でダウンロード (391K) -
原 一郎1985 年 51 巻 12 号 p. 1939-1945
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe flight surveys were conducted five times during the day in the waters off the southeast coast of Hokkaido where a big catch of sardine Sardinops melanosticta has been taken. In the area sardine schools were easily sighted, but were only distinguished by dark color against lighter background of the sea. To examine a moving direction of school, an experiment was conducted near the fishing ground. In the experiment, it was carried out that floaters fixed to a seamarker were airdropped near a school, and after relative changes between the school and the seamarker were compared each other. The visual observations were conducted 4 times around the fishing ground. The shape of the schools was observed by sight and the number of schools was counted.
From the result of the experiment, it is concluded that the front of the school, which indicates the direction of movement, is dark blue color and the rear is watery blue color. That is, by observing the shape and tone or shade of color of a school, we can easily estimate the moving direction of schools.,
During the visual observations, elongate shape schools have been frequently observed. It is estimated that there were much continuous immigrations from the outside of the small fishing area during before and after the surveys.抄録全体を表示PDF形式でダウンロード (425K) -
加藤 憲司1985 年 51 巻 12 号 p. 1947-1953
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThough a large number of rainbow trout had been stocked in Japan, after the first introduction from the United States in 1877, establishment of natural population is seldom witnessed.
In the three tributaries of upper Tama River drainage, rainbow trout believed to be naturally spawned (60 to 173mm in total length) and spawning redds were recognized from 1976 to 1984. A study was made on the analysis of environment of the streams and origin of rainbow trout caught there to determine the factors which made natural spawning possible. Since such small fish nor eggs were stocked there, they were believed to be definitely the result of natural spawning.
Rainbow trout believed to be naturally spawned and spawning redds were not recognized in the ordinary fishing area (catchable fish stocked once just before opening of season), but recognized only in or near the special fishing area (catchable fish stocked daily or weekly throughout season). Though almost all of the fish stocked into ordinary area are removed within several days of opening of season, there are recognized surviving fish in the special area even after close of season. So, rainbow trout believed to be naturally spawned might owe their origin to the survived parent fish stocked into special area.
The rainbow trout are stocked into most of the streams in Japan through the same way as done for the ordinary area of the Tama River. So one of the important reasons of scarce establishment of natural rainbow trout population in Japan is supposed to be removal of stocked fish by high angling pressure.抄録全体を表示PDF形式でダウンロード (577K) -
松宮 義晴, 増元 英人, 田中 克1985 年 51 巻 12 号 p. 1955-1961
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーIn order to clarify the ecology with the river ascension of the larval and early juvenile Japanese sea bass Lateolabrax japonicus, larva net sampling and environmental observations were made in the Chikugo River estuary of Ariake Bay. A total of 3297 fish (SL, 10-24mm) was collected with 274 tows in 1981-1983.
Preliminary notes on the ascending mechanism of the sea bass are given. In the vertical research across the river, the ascending sea bass larvae were concentrated near the bank, and the catch on flood tide is larger than on ebb tide both in the middle and near the bank of the river. The catch in the surface layer is more than that at the bottom (the ratio, 1:0.55).
Comparative examination of yearly abundance and mean length was made, including results in mid 10-day of March during 1979-1983. There was a 10-fold difference in yearly abundance around the river mouth area. The range of yearly variation in the mean body length was more than 2mm.
Ages and spawning season were determined from daily growth increments in the otoliths. One increment was formed each day after hatching. Spawning season extended from November to the next February, and the peak spawning was in January. The mean hatching date slightly changed each year. Estimated standard length was almost the same for sea bass of the same age (100 days after hatching). The sample shrinkage by preservation and percentage of water contents was also examined.抄録全体を表示PDF形式でダウンロード (1518K) -
正木 康昭, 伊東 弘, 東海 正, 山口 義昭1985 年 51 巻 12 号 p. 1963-1970
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーFor the studies of age and growth of finespotted flounder (Pleuronichthys cornutus, TEMMINCK et SCHLEGEL), age determination was carried out by counting the number of zones in the otolith from 12, 314 specimens caught in Suõ-nada area of Seto Inland Sea from October 1980 to October 1984.
On the basis of the monthly changes of the appearance rate of hyaline zone at the margin of the otolith of 3, 268 specimens, hyaline zone is found once a year during the months from August to March, mainly December. The spawning season extends from November through December. Therefore, spawning roughly coincides in time with formation of hyaline zone, and the mean standard body length at the zone formation may be concluded as that at each full year of age.
As the above mentioned results, each male and female finespotted flounder grows 109, 108mm in standard body length at one year of age, 131, 139mm in that at two years of age, 140, 154mm in that at three years of age, 146, 162mm in that at four years of age, and 149, 168mm in that at five years of age, respectively.
No difference in growth of finespotted flounder is found between sexes until one year of age, however, female grows faster than male on and after one year of age.
The age determination based on the otolith zones of finespotted flounder on and after five years of age is considerably difficult because of the difficulty of discrimination of opaque and hyaline zones in the otolith.抄録全体を表示PDF形式でダウンロード (452K) -
船越 将二, 和田 浩爾, 鈴木 徹1985 年 51 巻 12 号 p. 1971-1975
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe weight of red and white muscles, the number of vessels in 1mm long segment o rete and muscle temperature were measured to elucidate the development of the ability to conserve metabolic heat during the growth of young bluefin tuna Thunnus thynnus (20-39cm in fork length).
Maximum weight of boiled red and white muscles was located in the region of 40 to 55% FL (percent of fork length from the snout), where number of rete vessels and body thickness were maximum. Also, maximum muscle temperature was found at the same region in the body.
Both boiled red and white muscle masses markedly increased, but the former was superior in the rate of increase to the latter. The number of rete vessels conspicuously increased, too. It was considered that the increase in the number of rete vessles contributed not only to transporting more blood volume in proportion to increase of red muscle mass, but also to improving the efficiency of heat conservation. Indeed, maximum muscle temperature tended to be higher and
distribute more broadly in larger specimens. Maximum muscle temperature of the specimens of 32.1-39cm in fork length were maintained 3-4°C over environmental seawater temperature of 24.2°C. Accordingly, it was appeared that the ability to conserve metabolic heat had already been developing.抄録全体を表示PDF形式でダウンロード (350K) -
兼廣 春之, 鈴木 誠, 松田 皎1985 年 51 巻 12 号 p. 1977-1982
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーSchooling behavior of rose bitterling Rhodeus ocellatus ocellatus was examined by a water tank experiment. The experiment was performed for the different size of fish school (n=1 to 5). A video behavior analyzer system was used for observation and analysis of the fish behavior.
From statistical analysis and power spectral analysis of separation distance between fishes and swimming speed it was found that some differences were seen between fish group less than two and fish group larger than three. The results suggest that the schooling behavior is influenced by the group size and the minimum size of fish schooling in order to maintain a stable schooling behavior is three fishes.抄録全体を表示PDF形式でダウンロード (382K) -
兼廣 春之, 鈴木 誠, 松田 皎1985 年 51 巻 12 号 p. 1983-1988
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーWater tank experiments of fish behavior to model net were made by using Rhodues ocellatus ocellatus. Trap net with a leader net, play-ground and two bag nets was used as the model net and its function was discussed in conjunction with fish behavior. Also the entering and leaving behavior of fish to the model trap net was observed and analyzed by using a video position analyzer system.
Results of analysis of fish behavior showed that the average swimming speed and separation distance between fishes were different in each part of the net in the following decreasing order; outside of the net>inside of the net (play-ground)>inside of the bag net.
It was suggested that the difference of the fish behavior before and after entering the net was closely connected with the different functions (swimming space and the construction) of each part of the net.抄録全体を表示PDF形式でダウンロード (414K) -
津田 良平, 鷲見 栄一1985 年 51 巻 12 号 p. 1989-1996
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe characteristics of current velocity near the sea bottom around fish-cultivated ground and the variation of turbidity related to its velocity were examined. Current velocity near the sea bottom below fish-cultivated cages was relatively faster than that of other stations. This result seemed to be caused by the mechanism of hydrodynamics. The power spectral density of current velocity near the sea bottom and of turbidity had peaks of 0.0362 and 0.0725cycle/min and the periodicities are 28 and 14min, which corresponded to the periodic components of seiche in Uragami Bay. It was obvious that the variation of turbidity near the sea bottom around fish-cultivated ground was influenced by the short periodic component in seiche.抄録全体を表示PDF形式でダウンロード (455K) -
井上 悟, 佐藤 修1985 年 51 巻 12 号 p. 1997-2006
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーIt is important to determine the added mass of objects to estimate the hydrodynamic force on floating fisheries banks. The following is a report on experimental results obtained for the added masses of cylinders and circular pipes which are limited in length. The apparatus used in this experiment is essentially a torsion pendulum which has been improved by the authors. The values of added masses are calculated from the difference between the periods of this pendulum in water and in air. By using a phosphore bronze plate to which strain gages were affixed for this pendulum, it was possible to pick up the torsion electrically and to put record into computer. In this way, the experiment was simplified and the treatment of the data was very efficient. Objects used in this experiment were cylinders varying in diameter and length and circular pipes varying in diameter, length, and thickness.
We were able to determine the added masses of these objects.抄録全体を表示PDF形式でダウンロード (1473K) -
松浦 基, 吉岡 武也, 田中 晴生, 新井 健一1985 年 51 巻 12 号 p. 2007-2012
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーChanges in some biochemical activities and filament formation ability of tilapia myosin during heat treatment were studied.
The Mg-ATPase activity and superprecipitation activity of tilapia actomyosin constituent, increased in the very early period of heat treatment of tilapia myosin. On the other hand, tilapia heavy-meromyosin (HMM) showed a decreasing Mg-ATPase activity in the presence of actin, as the time of heating was prolonged.
Electron microscopy revealed that the filaments of tilapia untreated myosin were much shorter than those of rabbit untreated myosin, and filaments of tilapia myosin became thicker and longer in the early period of heat treatment.
On the basis of these findings, it was strongly suggested that the changes in actin-activated Mg-ATPase and superprecipitation activities of tilapia actomyosin are closely correlated with those in the physiological state of myosin filaments.抄録全体を表示PDF形式でダウンロード (1356K) -
小林 邦男, 中村 良子, 今田 信良1985 年 51 巻 12 号 p. 2013-2017
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーA study was made of the identification of the conjugated metabolites of fenitrothion [O, O-dimethyl O-(3-methyl-4-nitrophenyl)phosphorothioate; Sumithion] formed by tiger shrimp Penaeus japonicus, and also the accumulation of the metabolites in the shrimp with exposure time.
After benzene-extraction from the homogenate of shrimp exposed to 0.5ppb [14C]fenitrothion, some conjugated [14C]metabolites remaining in the residues were extracted with ethyl ether. The identification and determination of the conjugated [14C]metabolites were performed by an enzymatic analysis, using β-glucuronidase, β-glucosidase and arylsulfatase. The conjugated [14C]metabolites were identified as the sulfate and β-glucoside of [14C]3-methyl-4-nitrophenol. The amounts of both the conjugates of sulfate and β-glucoside accumulated in the shrimp increased with exposure time and reached 60 and 36pmol/g body weight in 24-h exposure period, corresponding to 58 and 38% of the total conjugates, respectively. However, glucuronide was not detected in the shrimp.
It is very interesting from the view point of comparative biochemistry that the shrimp forms both conjugates of sulfate and β-glucoside, instead of the glucuronide, as well as insects which belong to the same phylum of Arthropoda.抄録全体を表示PDF形式でダウンロード (409K) -
中山 照雄, 間所 雅之, 丹羽 栄二1985 年 51 巻 12 号 p. 2019-2025
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーPollack fibers were produced by extrusion and steaming. The texture of fibers was evaluated by their shear values. Shear stress and volumetric flow rate were measured during the extrusion of minced fish paste, and the data was fitted to flow equations by FACOM 230-38S to find the bestfit rtheological parameters. The velocity profile of minced fish paste through a nozzle followed the power-law flow model with yield stress. Texture of pollack fibers was predicted, using these rheological parameters of minced fish paste. Apparent viscosity K was most useful among these parameters, and nearly proportional to shear value of pollack fiber. As apparent viscosity K of minced fish paste was gradually increased by the addition of egg white or whey protein up to 15%, shear value of pollack fiber was also increased. As apparent viscosity K of minced fish paste was increased by the decrease of added water, shear value of pollack fiber was also increased. Any reasonable relationship between apparent viscosity K of minced fish paste and shear value of pollack fiber was not found on the effect of NaCl addition.抄録全体を表示PDF形式でダウンロード (576K) -
滝井 健二, 示野 貞夫, 竹田 正彦1985 年 51 巻 12 号 p. 2027-2031
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーSequential changes of digestive enzyme activities in gastrointestinal tissue and their contents were examined at 0, 1, 3, 5, and 12h after feeding a paste diet flavoured with the feeding stimulants: alanine, glycine, proline, and histidine. Protease activity in gastric tissue decreased soon after feeding, reaching a minimum level at 3h. The activity in gastric contents maintained low levels in contrast to the tissue. Protease activity in intestinal tissue decreased immediately after feeding and reached a minimum level at 5h. The intestinal content's activity increased after feeding, reaching a maximum level at 5h. Amylase activity in intestinal tissue was approximately constant during the 12h, but the activity in their contents slight increased with time. There was a significant correlation observed between the mount of the intestinal contents and the total activity of protease or amylase in the contents. This suggested that the digestive enzymes were secreted in proportion to the amount of contents in the eel intestine.抄録全体を表示PDF形式でダウンロード (340K) -
生野 芳博, 清水 稔, 越野 泰代, 眞岡 孝至, 松野 隆男1985 年 51 巻 12 号 p. 2033-2035
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe carotenoids in the integuments of yellow-tail rockfish Sebastes flavidus were investigated from the stereochemical points of view. Tunaxanthin fraction was found to be a mixture of three stereoisomers, tunaxanthin C (1), tunaxanthin D (2), and tunaxanthin J (3). Furthermore (6R, 6'R)-ε, ε-carotene-3, 3'-dione (4), (6R, 6'S;meso)-ε, ε-carotene-3, 3'-dione (5), and (6S, 6'S)-ε, ε-carotene-3, 3'-dione (6) Were also isolated.抄録全体を表示PDF形式でダウンロード (201K) -
須山 三千三, 平野 敏行, 鈴木 健1985 年 51 巻 12 号 p. 2037-2041
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe decomposition of carnosine, anserine, and balenine during storage of the big-eye tuna and the sei whale meats was studied with a view to ascertain the formation of methyl derivatives of histamine and β-alanyl-histamine. A chromatographic deteiination of these amines on a 15cm column of a cation exchange resin (JEOL, LCR-2) was achieved using a borate buffer of pH 10.2 having sodium concentration of 0.32N, at 52°C. When the big-eye tuna meats with and without addition of chopped sardine skin were stored at 5°C for 12 days or 20°C for 5 days, and when the sei whale meats with and without addition of mackerel slime at 25°C or 37°C for 3 days, the meats turned putrid in the late stages of the storage periods. The amounts of anserine in the big-eye tuna, and carnosine and balenine in the sei whale did not decrease. Although a lot of histidine was decarboxylated into hislamine in the big-eye tuna meat at 20°C, the methyl derivatives of histamine and β-alanyl-histamine were not detected.抄録全体を表示PDF形式でダウンロード (351K) -
須山 三千三, 平野 敏行, 鈴木 健, 奥積 昌世1985 年 51 巻 12 号 p. 2043-2046
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーSeventeen strains of bacteria isolated from the putrefied meats of the sei whale and mackerel were incubated in the media containing balenine, and it was found that they are able to hydrolyze balenine in a greater or lesser degree. Among 68 strains of histamine-forming bacteria isolated from the sardine and mackerel, on the other hand, only 2 strains showed the hydrolyzing activities on carnosine or anserine when incubated in media having these imidazole dipeptides. β-Alanyl-histamine and its methyl derivatives which correspond to the decarboxylation products of carnosine and its methyl derivatives were not detected throughout the experiments.抄録全体を表示PDF形式でダウンロード (260K) -
渡辺 尚彦, 武輪 正彦, 高井 陸雄, 酒井 愿夫1985 年 51 巻 12 号 p. 2047-2050
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe cooking rates of the bone of mackerel were experimentally examined at various temperatures in distilled water and in aqueous acetic acid solution. The degree of uncooking was determined by the hardness modulus, F/a, which is the maximum force applied in shearing the sample, F, divided by the maximum horizontal length of the cross section of the sample, a. Since the cooking of the mackerel bone could be described by an expression like a first order reaction, the apparent cooking rate constants were obtained in the usual manner. Arrhenius plot gave parallel straight lines at successive intervals according to acetic acid concentrations. The activation energy, 22.2kcal•mol-1, was obtained from the gradient of the straight lines. It was suggested that an increase of the cooking rate by acetic acid is attributable to a rise of frequency factor. Finally, the power of acetic acid in making the cooking time shorter was shown.抄録全体を表示PDF形式でダウンロード (299K) -
原田 勝彦, 秋島 幸夫1985 年 51 巻 12 号 p. 2051-2058
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe attraction-effective fractions obtained from the whole frond of brown algae Ishige okamurai were further fractionated to several subfractions and these subfractions were examined on the attraction activities for black abalone Haliotis discus. The subfractions of 40 and 50% ammonium sulfate saturation-proteins, acidic and basic amino acids, and neutral and phospho-lipids were high in these activities.
In this context, various chemicals of proteins, amino acids and lipids, and nitrogenous bases, were investigated for the activities. Of proteins tested, human albumin, and α- and β-globulins were ascertained to be active attractants: albumin was most effective. Of L-amino acids, all basic amino acids, some neutral and acidic amino acids, amides, and imino acids were also ascertained to be active attractants, particularly ornithine and hydroxyproline being most effective. On the other hand, L-amino acids proved to be effective attractants, as opposed to D-ones. Of fatty acids and lipids, tristearin was high in the activity and the majority of phospholipids were effective, especially phosphatidyl-inositol being highest in the activity. Of nitrogenous bases, all volatile methylamines and ethylamines tested and some non-volatile ones were ascertained to be active: monomethylamine and γ-aminobutyric acid were most effective attractants.抄録全体を表示PDF形式でダウンロード (578K) -
土屋 隆英, 設楽 弘之, 野間口 恵子, 佐野 猛, 松本 重一郎1985 年 51 巻 12 号 p. 2059-2065
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーα-Actinin was isolated from squid mantle muscle. The α-actinin had the molecular weight of 163, 000 and was a homo-dimer. The amino acid composition of squid α-actinin was somewhat different from those of α-actinins from other sources. Squid α-actinin raised the viscosity of F-actin solution, formed bundles of F-actin filaments side by side, and promoted the Mg++-ATPase activity of desensitized actomyosin. These effects were reduced by the presence of tropomyosin. These properties of squid α-actinin were similar to those of α-actinins from other sources. We speculate that α-actinin exists in dense bodies and plays a role in fixing thin filaments to dense bodies.抄録全体を表示PDF形式でダウンロード (709K) -
立田 穣, 平野 茂樹, 小柳 卓1985 年 51 巻 12 号 p. 2067-2072
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーThe retention and excretion by black-fish Girella punctata, of 59Fe taken up from seawater or food organisms were observed by tracer experiment. The highest concentration factor was shown by the seaweed, Enteromorpha sp. among the prey and predator organisms. In the uptake route of food, the contamination mode of food and food species had effect on the retention ratio of radionuclide, showing that 59Fe assimilated in food was more difficult for the uptake than 59Fe solution fed with food, and 59Fe in seaweed is more difficult than 59Fe in worm. The distribution ratio of radioiron in the body organ showed large distribution in digestive tract regardless of uptake route and contamination mode of food. GFC profile showed that the combining form of radioiron are same as in all organs.抄録全体を表示PDF形式でダウンロード (465K) -
周 照仁, 落合 芳博, 橋本 周久1985 年 51 巻 12 号 p. 2073-2078
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーMyoglobin (Mb) was isolated from the dark muscle of bluefin tuna Thunnus thynnus by Sephadex G-75 gel filtration and CM-Sephadex C-50 ion-exchange chromatography. This Mb was dissolved in 50mM phosphate buffers of several pH values, and examined for the rate of autoxidation (metMb formation) under various conditions of freezing and thawing.
When frozen quickly and kept at -80°C for 2h and then thawed, the Mb solution at pH 6.5 gave rise to a metMb formation rate of 5-15%, whereas the solutions at lower or higher pH values gave much higher rates of up to 70-80%. In contrast, metMb formation exceeded 80% regardless of pH values when frozen slowly at -20°C and then thawed after 20h.
Thawing temperatures (0-20°C) affected the autoxidation rate of Mb to a less extent, though the higher the temperature, the slower the metMb formation.抄録全体を表示PDF形式でダウンロード (1804K) -
太田 冬雄, 岡澤 康夫1985 年 51 巻 12 号 p. 2079
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (97K) -
杉田 治男, 出口 吉昭1985 年 51 巻 12 号 p. 2081
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (91K) -
鈴木 譲1985 年 51 巻 12 号 p. 2083
発行日: 1985/12/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (103K)
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