巻号一覧
51 巻, 9 号
選択された号の論文の26件中1～26を表示しています
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酒井 清, 野村 稔, 隆島 史夫1985 年 51 巻 9 号 p. 1395-1399
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーIn order to identify factors for the variations in egg quality by individual brood fish, egg samples taken from the lst Group (three-year olds in the 10-ton pond) and the 2nd Group (two-to twelve-year olds in the 4, 000m2 pond) were examined for their respective characteristics. In addition to normal eggs and dead eggs, three types of abnormal eggs were found among the samples; namely, (i) wrinkly eggs which developed wrinkles on the egg membrane, (ii) spotted eggs which developed minute black spots on the egg cortex, and (iii) plasma-bulged eggs which developed swellings of cortical ooplasma. The percentage of abnormal eggs was higher in the 2nd Group than the 1st. Moreover, the percentage varied greatly by individual brood fish, reaching well over 60% in some cases.抄録全体を表示PDF形式でダウンロード (1180K) -
北村 等, 平山 和次1985 年 51 巻 9 号 p. 1401-1405
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーColonies attached to glass slides were suspended in the sea. They were also immersed in low salinity waters once for one or two hours or repeatedly for one, two or six hours once a day to examine the effects of salinity changes on growth. The effect was also examined with a culture method in beakers using water of various salinity (33.7, 31.0, 27.9, 24.1 and 18.1‰), with colonies on glass slides with Rhodomonas as food.
Exposure to fresh water for even a short time caused severe damage to the animal, but salinities higher than 9‰ were not injurious for their further growth. Colonies repeatedly exposed to seawater lower than 20‰ will not grow to be a mature colony. If the seawater has a salinity higher than 25‰ they might grow to a mature stage and in salinities higher than 30‰ they grow well.抄録全体を表示PDF形式でダウンロード (357K) -
讃岐 田訓, 岡本 弘子, 人見 宗男1985 年 51 巻 9 号 p. 1407-1417
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe surveys were carried out at 22 stations in Lake Hamana including the satellite lakes from June through October, 1981 and in April, 1982. The three regions of the lake that were divided by the degrees of median diameter (Mdφ) of the sediments coincided with the regions characterized by the other properties of the sediments and also by the macrobenthic community. Region A; The southern half of the main lake and the southern parts of Lake Shonai with the phi values of sediments of less than 4φ, Region B; The central parts of Lake Shonai with those of 4 to 8φ, and Region C; The northern half of the main lake with the two adjoining lakes and the inner parts of Lake Shonai where the values were more than 8φ. The fauna never became afaunal in regions A or B. However, in region C, the concentration of organic substances in the sediments was extremely high. And the mean values of bottom dissolved oxygen contents of less than 1.4mg/l and redox potentials in the sediments of less than -150mV were observed in June in the region where the macrobenthic fauna was poor, and became completely extinct from July through Setpember. The afaunal state continued till October. Therefore, those levels of dissolved oxygen contents and redox potentials of the bottom in June could be numerical indicators of the onset of the extinction of macrobenthic fauna. The causes which would result in the extinction of the fauna were also discussed in connection with the formation of a thermo-halocline in region C.抄録全体を表示PDF形式でダウンロード (771K) -
余座 和征, 添田 秀男, 嶋村 哲哉, 長谷川 英一, 吉原 喜好1985 年 51 巻 9 号 p. 1419-1423
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーBiotelemetry system was used to investigate the horizontal swimming behaviour of chum salmon Oncorhynchus keta in early migratory season at Okhtsk Sea off the coast of Shiretoko Peninsula, Hokkaido, and the data of two examinations were analyzed.
In both examinations, the direction of horizontal migration was north-east about along the peninsula.
The average of horizontal swimming speed from start to end of pursuit was 0.92m/s (1.23FL/s). The maximum speed measured at one h intervals was 2.43m/s (3.42FL/s).抄録全体を表示PDF形式でダウンロード (343K) -
添田 秀男, 余座 和征, 嶋村 哲哉, 長谷川 英一, 吉原 喜好1985 年 51 巻 9 号 p. 1425-1429
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーBiotelemetry system was used to investigate the vertical moving behaviour of chum salmon Oncorhynchus keta in early migratory season at Okhtsk Sea off the coast of Shiretoko Peninsula, Hokkaido, and data of two examinations were analyzed.
The average of swimming depth was 85.3m and 23.8m. The estimated maximum value was 350m.
On the vertical moving speed, the average of diving speed was a little faster than that of rising, and the maximum speed observed was 0.57m/s when diving.抄録全体を表示PDF形式でダウンロード (299K) -
中井 敏博1985 年 51 巻 9 号 p. 1431-1436
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーResistance of an eel pathogen Pseudomonas anguilliseptica to bactericidal action of fish sera was compared between K antigen-possessing (K+) and -lacking (K-) strains in connection with their pathogenicity. The K+ strain displayed high resistance to the bactericidal action of Japanese eel, European eel, and bluegill sera, but not to carp, goldfish, rainbow trout, or tilapia sera, whereas the K- strain was highly sensitive to all sera. The pathogenicity tests proved that the former three species were susceptible to the virulent K+ strain, but the latter four were not. Thus, the K antigen-related resistance to serum-killing corresponded with the pathogenicity of the organism. The bactericidal activity of fish serum was more effective at 28°C than at 20°C, and was lost by heat-treatment at 50°C for 30 min.抄録全体を表示PDF形式でダウンロード (446K) -
中原 紘之, 上野 正博1985 年 51 巻 9 号 p. 1437-1440
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーEarly successional species (ESS) occupy most open spaces on rocky shores due to the abundance of their reproductive cells in the water. Late successional species (LSS) also invade these open spaces but occupy a smaller space because of the small number of their reproductive cells in the water. Within a few days, however, most of the settled reproductive cells and their germlings are lost, and ESS occupy most of the open space. There is little evidence that ESS modify the environment or inhibit the invasion of LSS. In most cases, ESS exist alternately with LSS. In some places, this alternation of species does not occur and ESS dominance continues.
A model of this system has been proposed. The “survival rate model” suggests that the mortality and the ratio of reproductive cells of both species governs whether the alternation from ESS to LSS occurs or not. We show that the relationship between the ratio of reproductive cells of LSS to ESS, r, and the ratio of mortality of LSS to ESS, m, is very important for determination of succession in algal community. If ESS occupy 1/(r+1) of area, when more open space becomes available, ESS will occupy m/(r+m) of that new area. When r is constant and m becomes smaller, the spece occupied by ESS becomes smaller. So succession occurs when m is larger than 10-1×r. However, when the mortality rates of ESS decrease and the value of m is r or more, ESS are dominant.抄録全体を表示PDF形式でダウンロード (375K) -
梅崎 勇1985 年 51 巻 9 号 p. 1441-1445
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe growth of length and weight of the stem and the relationship between the branching pattern of the stem and annual rings of the holdfast in Sargassum ringgoldianum HARV. subsp. coreanum (J. AG.) YOSHIDA (Sargassaceae, Phaeophyceae) growing in Obama Bay, a branch bay of Wakasa Bay located in the middle part of Honshu facing the Japan Sea were studied. The stem is dichoto-mously branched several times, thus the lowest axis and the dichotomies above the axis were named as stemsI, II, III, IV, and V in the order of branching from base to apex. Among the branches, stem I is the longest and heaviest and becomes longer and heavier with the age of the plant. The mean length and weight of stem I were 10.19cm (S. D. 4.09cm) and 1.54 g (S. D. 1.07g), respec-tively. Statistically, a total length of all branches of stem V plants measures 109.1 cm and its corresponding weight is 5.1g. Annual rings of the holdfast showed a direct relationship to the number of dichotomies. Stem I1 plants are one year old; stem I2 plants two years old; stem H plants three years old; and stem III plants four years old. This relationship was obscured in stem IV and stem V plants, because they were in various stages of growth and their ages varies from four to six years old. From these data it is shown that it is possible to determine the plant age of S. ringgoldianum from the branching of the stem without cutting the holdfast.抄録全体を表示PDF形式でダウンロード (327K) -
小栗 幹郎1985 年 51 巻 9 号 p. 1447-1449
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーUsing twenty species of marine teleosts, histological observations were made on the interstitial tissue of kidney to examine amount and distribution of yellow and dark brown pigment granules detectable in the tissue. The results obtained are as follows. In nine species such as Japanese parrot fish and kelp-bass, the pigment granules were detected as small deposits in the nterstitial tissue, whereas in eleven species such as Japanese horse mackerel and yellowtail, the granules were observed as comparatively large aggregation in the tissue. In both cases the following three types were distinguished on pigment composition. In the first type the pigment deposition was made up only of yellow pigment granules, in the second the deposition consisted of yellow and dark brown pigment granules, and in the third type the pigment aggregation was composed only of the latter pigment granules.抄録全体を表示PDF形式でダウンロード (1183K) -
張 成年, 藤尾 芳久1985 年 51 巻 9 号 p. 1451-1460
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーTwo types of the fresh water shrimp Palaemon paucidens DE HAAN inhabiting the Natori River system (Japan) were genetically separated by starch gel electrophoresis. One type, A, consists of the individuals exhibiting either GpiA, MpiA or Mdh-2A but not Mdh-1c genes, while the other type, B, does not exhibit GpiA, MpiA or Mdh-2A but shows Mdh-1c in moderate frequency. No evidence of hybridization between A and B types was observed even in areas where both were found together throughout the breeding season. Significant differences between the A and B types were found, i.e., the individuals of A type lay large eggs and most of them possess 2 lower rostrum teeth, while the individuals of B type lay small eggs and most of them possess 3 lower teeth.抄録全体を表示PDF形式でダウンロード (1308K) -
小畠 渥, 積山 昇司, 山本 康之1985 年 51 巻 9 号 p. 1461-1468
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーExtractive nitrogenous constituents of dark muscles were determined in 13 species of fish having deep-seated dark muscle (skipjack, frigate mackerel), fin dark muscle (blow fish, surgeon fish) and superficial dark muscle (nine other species) and compared them with those of white muscles.
The concentration of total extractive nitrogen and creatine were high in the dark muscle as compared with the white muscle. Of the ninhydrin positive substances, taurine concentration was much higher in the dark muscle than in the white muscle, and His, Ala, Lys and Glu were also high in the dark muscle. But His was higher in concentration in the white muscle of pelagic fishes, Except in larval fish, taurine content in the dark muscle of the common mackerel did not vary according to body weight or lipid content. Using chromatography for determining the ninhydrin positive substance in the dark muscle, a few unidentified peaks appeared near the taurine peak. Trimethylamine concentration was higher in dark muscle. But the volatile basic nitrogen concentration was less in the dark than in the white muscle. The total amount of nucleotides and related compounds was less in dark muscle than in white muscle. But the ratio of nucleotides to the total extractive nitrogen was larger in the dark than in the white muscle. Therefore, it was thought that no difference in quantity of nitrogen was found between the two kinds of muscles. However, constitutively, there was large difference in the inosin monophosphate concentration in the dark muscle than the white muscle.抄録全体を表示PDF形式でダウンロード (491K) -
Shanta NAIR, 塚本 久美子, 清水 潮1985 年 51 巻 9 号 p. 1469-1473
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーHeterotrophic bacteria were isolated from seawater, particulate matter and plankton of Tokyo, Sagami and Suruga Bays and the bacteriolytic activities of the isolated bacteria were investigated. The percent of bacteriolytic strains among the seawater isolates was 24% at Tokyo Bay and 50.7% and 51.7% at Sagami and Suruga Bays. The percentage of bacteriolytic strains was higher among the bacteria isolated from zooplankton than those from the seawater. The difference was most prominent at Tokyo Bay. More than 60% of the isolates from zooplankton produced lytic enzymes. The generic composition of bacteriolytic bacteria varies with area and the source of isolation. Pseudomonas were the major bacteriolytic group at Sagami and Suruga Bays, whereas it was Vibrio at Tokyo Bay. Among the isolates, from zooplankton in AburatsuboInlet, Vibrio was also the major group that produce lytic enzymes. Most of the bacteriolytic strains attacked Gram negative bacteria (V. parahaemolyticus) and only 5% of them showed lytic activity to Gram positive test organism (S. aureus).抄録全体を表示PDF形式でダウンロード (340K) -
高橋 是太郎, 蝦名 秀昭, 江木 衷, 松本 幸三, 座間 宏一1985 年 51 巻 9 号 p. 1475-1486
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーDorsal muscle phosphatidylcholine (PC) molecular species of sardine, mackerel, big-eyed tuna, brown sole, sand flounder, rock fish, Alaska pollack, chum salmon, blue shark, mackerel shark, carp and rainbow trout were analyzed on HPLC.
Flat fish such as brown sole and sand flounder were extremely characteristic, since these fish contained (16:0) (20:5) as the most prominent molecular species unlike the rest of the fish that had (16:0) (22:6) as the most prominant molecular species.
Principal component analysis (PCA) of the PC molecular species suggested that except for sardine dark muscle, chum salmon (feeding migration stage) and rainbow trout, a common correspondant movement against seasonal change in the content of (20:5) (20:5) was observed in the majority of these fishes.抄録全体を表示PDF形式でダウンロード (596K) -
高橋 是太郎, 江木 衷, 座間 宏一1985 年 51 巻 9 号 p. 1487-1493
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーMolecular species of muscle phosphatidylcholine (PC) of chum salmon of four different migratory stages were analyzed on HPLC.
By introducing principal component analysis (PCA) for the interpretation of the results, interesting movements were observed on the first and second, as well as the first and third principal component planes in accordance with the advance in migration. The plots of male and female moved almost together on these planes.
It was clearly seen that molecular species of (16:0) (22:6) drastically decreased when the upstream stage in spawning migration begins.抄録全体を表示PDF形式でダウンロード (359K) -
磯 直道, 水野 治夫, 斎藤 隆英, 林 慶陽, 藤田 孝夫, 長久 英三, 王 慥1985 年 51 巻 9 号 p. 1495-1499
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe effect of adding otoshimi on the protein network structure within kamaboko was examined by measuring jelly strength, rupture strength, elastic modulus, viscosity, relaxation time, and folding score. It became clear that the tenderness of the sample increased by increasing the otoshimi contents. This suggests that the network structure becomes weaker with the addition of otoshimi. On the basis of the theory of rubber elasticity, it was concluded that the number of chains per unit volume (mol/cm3) decreased from ca. 1.7×10-4 to ca. 1.2×10-4 depending on the otoshimi content (0-100%).抄録全体を表示PDF形式でダウンロード (311K) -
渡辺 武, 伊藤 暁, 佐藤 秀一, 北島 力, 藤田 矢郎1985 年 51 巻 9 号 p. 1501-1509
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーBoth buoyant (normal eggs floating on water surface) and deposited (abnormal eggs that sink to the bottom of tanks) eggs produced by broodstock of different dietary histories were analysed for lipid class, fatty acids, vitamins A and E, cholesterol, minerals, and proximate composition. The dietary treatments were diets containing different levels of protein which were fed for 6 months, or broodstock fed diet supplemented with pigments and fat-soluble vitamins or frozen raw krill shortly before spawning.
There were no marked differences in proximate and mineral composition due to the different broodstock diets, although the concentration of protein in the buoyant eggs was proportional to dietary protein levels used, while moisture content varied inversely. The fatty acids in eggs were greatly affected by dietary fatty acids supplied to broodstock either shortly before their spawning or even during spawning. In the eggs from the broodstock fed on corn oil diet immediately, and prior to spawning, the percentage of 18:2ω6 was as high as 26% in comparison with the original value. Vitamin E was also found to be easily incorporated in the eggs together with lipids via the diet. The level of cholesterol in eggs was almost constant irrespective of the cholesterol levels in the diets.
Thus no marked differences that might related to egg quality were observed in chemical components in either buoyant or deposited eggs among the experimental groups, although significant differences occurred between the buoyant and deposited eggs.抄録全体を表示PDF形式でダウンロード (833K) -
渡辺 武, 小泉 匡, 鈴木 秀和, 佐藤 秀一, 竹内 俊郎, 吉田 範秋, 北田 哲夫, 塚島 康生1985 年 51 巻 9 号 p. 1511-1521
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーRed sea bream broodstock were fed on various diets of different nutritional quality for either 26 days or shortly before spawning to clarify the effect of the duration of the period over which diets are fed on spawning and egg quality.
The percentage of buoyant eggs in the control group receiving the white fish meal diet was 42.7%, slightly lower than that hitherto obtained by the diet. Addition of 200mg of DL-α-toco-pheryl acetate to the diet elevated the value to 77.9%. The value was also effectively improved by replacement of cuttlefish liver oil with krill oil extracts. Substitution of cuttlefish liver oil by corn oil resulted in deterioration of egg quality. In the eggs produced by the broodstock fed on a diet containing cuttlefish meal as a protein source, percentage of buoyant eggs was as high as 98.4%. Feeding broodstock with frozen raw krill after the fish had previously been fed on a diet fortified with vitamin E for 26 days also resulted in elevation of the percentage of buoyant eggs. A similar improvement was also observed in a group given a diet containing corn oil, the original value was 30.6% it was elevated to 91.7% by feeding frozen raw krill shortly before spawning.
Consequently, total quantity of fish seed produced by each experimental group calculated from the number of buoyant eggs, hatchability and abnormality in both eggs and hatched larvae was highest in the cuttlefish meal group, followed by the frozen raw krill groups. Thus in fishes like red sea bream which can accept diet actively even during spawning, the quality of diets given to broodstock even shortly before spawning becomes very important for their reproduction.抄録全体を表示PDF形式でダウンロード (758K) -
林 賢治, 大川 寛, 川崎 賢一1985 年 51 巻 9 号 p. 1523-1526
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe content and composition of alkyl glyceryl ethers of liver lipids of gonatid squid Berryteuthis magister in the Sea of Japan were investigated.
Weight of the livers of thirty-four specimens of this species accounted for 9.0±1.8% of total body weight; 23.8±9.1% of the liver was lipids. The liver lipids contained high quantities of unsaponifiables (26.7±4.0%), consisting of 55.2±11.4% alkyl glyceryl ethers and 41.9±11.7% sterols. Alkyl glyceryl ethers and sterols originated from diacyl glyceryl ethers (27.5±9.0%) and steryl esters (12.0±5.6%), respectively. The relationship between the amount (Y, g) of alkyl glyceryl ethers of liver and liver weight (X, g) was expressed by the following equation: Y=-0.556+0.077X, (r=0.961, P⟨0.05).
The component alkyl glyceryl ethers were found to consist mostly of saturates (69.8±4.9%) and monoenes (27.4±4.7%) of 14-20 carbon numbers for the alkyl moiety. The principal components found were: chimyl (16:0, 58.7±6.4%), selachyl (18:1, 24.6±4.6%), and batyl (18:0, 6.6±1.9%) alcohols. This indicated that the liver of the gonatid squid species was a good source of alkyl glyceryl ethers having chimyl alcohol as its predominant component.抄録全体を表示PDF形式でダウンロード (325K) -
渡辺 尚彦1985 年 51 巻 9 号 p. 1527-1532
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe amount of direct and indirect energy input in the manufacture of fish meat paste product was estimated. The direct energy input was estimated to be 3390 kcal per kilogram of product (64%), and indirect 1882 kcal per kilogram of product (36%); namely, the total energy input was estimated to be 5272 kcal per kilogram of product. This figure is equal to or less than that for various other meat products. It has been said that sea food products are more energy intensive compared to other food products. However, the present results showed that this is not the case as far as the processing of finished product is concerned.抄録全体を表示PDF形式でダウンロード (443K) -
渡辺 尚彦1985 年 51 巻 9 号 p. 1533-1536
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe input of energy and non-energy goods in the manufacture of fish meal was examined. The amount of these goods was then converted into energy input. The estimated energy input for fish meal manufacture was 1, 600-2, 300 kcal per kilogram of product. Since this value is one half or one third of that for the production of starch or its related products, it can be said that fish meal plant is less energy intensive than other food processing plants. The total energy input, which is the sum of energy input in catching process and that in manufacturing process, was also estimated to be 4, 000-17, 000 kcal per kilogram of product. This result indicates that the fish meal made of sardine captured near Japan is more energy intensive than the maize which is transported for a long distance from USA as a principal constituent of assorted feed.抄録全体を表示PDF形式でダウンロード (270K) -
今田 千秋, 多賀 信夫1985 年 51 巻 9 号 p. 1537-1544
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe seasonal and spatial distribution of particulate protease (PPRase) activity in seawater from near to the shore to offshore areas around Japan were investigated.
The activities in Aburatsubo Inlet and Tokyo Bay were from one to two orders of magnitude higher than other offshore areas, suggesting that the active biological production causes high PPRase activity in the areas. The highest activity was observed in the upper layer of all the areas (Tokyo Bay, Sagami Bay, Off-Tateyama, and Oyashio regions) and decreased with depth.
In Aburatsubo Inlet, PPRase activity increased subsequently with the standing stock of primary producers from March to August and declined in the other months.
The population of protease-producing bacteria ranged from 104 to 107 per l, being approximately the same order of magnitude as that of the heterotrophic bacteria. Small populations of protease-producers were observed in Sagami Bay and Off-Tateyama, whereas considerably large populations of protease-producers were found in Aburatsubo Inlet and Tokyo Bay.抄録全体を表示PDF形式でダウンロード (425K) -
山澤 正勝, 志賀 一三1985 年 51 巻 9 号 p. 1545-1550
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーReducing sugars and glucose-6-phosphate added in Kamaboko were found to be effective to prevent H2S production in the retorted products. In contrast, sucrose, glucose-l-phosphate and sugar-alcohols which do not contain a free aldehyde group did not show similar effect. There-fore, it was estimated that the free aldehyde group of reducing sugars play an important role to prevent H2S production.
Among the reducing sugars, the pentoses (ribose, xylose) were the most effective, followed by hexoses (glucose, fructose) and disaccharides (maltose, lactose). On the hexoses, glucose was more effective than fructose.
However, the addition of reducing sugars caused brown color of the retorted products, and increasing the amount of them enhanced browning. Therefore, the proper quantity of reducing sugars to prevent H2S production without browning reaction was confirmed to be such con-centration levels as less than 0.005M for ribose and xylose, below 0.01M for glucose, below 0.05M for fructose, maltose and lactose. Under these conditions, the concentration of H2S in retorted Kamaboko was decreased from 35 to 70% against the control.抄録全体を表示PDF形式でダウンロード (468K) -
坂本 慎一, 関 伸夫1985 年 51 巻 9 号 p. 1551-1557
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーHydrolytic degradation of carp myosin with carp and rabbit calpains in 0.5M NaCl were followed by SDS-polyacrylamide gel electrophoresis and other methods. Carp myosin heavy chain was degraded restrictively into two subfragments (170, 000 and 43, 000 Mr proteins), carp calpain's activity being three times higher than rabbit calpain. In contrast, three light chains from this myosin were hardly degraded. Maximal hydrolysis as well as caseinolysis of this myosin occurred at lmM Ca+2 and at neutral pH. The hydrolysis showed an accelerated pace above 20°C. Rabbit myosin used for comparison was insusceptible to both calpains.
The two subfragments thus produced from carp myosin were separated from each other by differences in solubility. The soluble subfragment was composed of 170, 000 Mr protein and three light chains, and the insoluble was composed of 43, 000 Mr protein. The soluble subfragment exhibited a Ca2+-ATPase activity similar to the original myosin and the Mg2+-ATPase activity was accelerated by F-actin. It was concluded that carp myosin is degraded by either calpain into heavy and light meromyosin-like subfragments.抄録全体を表示PDF形式でダウンロード (1017K) -
沼倉 忠弘, 関 伸夫, 木村 郁夫, 豊田 恭平, 藤田 孝夫, 高間 浩蔵, 新井 健一1985 年 51 巻 9 号 p. 1559-1565
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーThe frozen surimi of Alaska pollack was ground with 3% salt. The salted paste was submitted to setting, so-called “SUWARI”, at a fixed temperature of 20°or 30°C. During the setting, a portion of the paste was taken out to assess the gel strength before and after heat treatment at 90°C for 40min. Another portion was used to solubilize protein of the paste with a medium containing SDS, urea and 2-mercaptoethanol, and to analyze the soluble protein by SDS-poly- acrylamide gel electrophoresis.
The solubility of the paste slightly decreased with the prolonged setting, whereas the disappearance of myosin heavy chain in it became evident when still in high solubility.
The increase in gel strength of both gels with and without heat treatment was accompanied by the decrease in myosin heavy chain and a concomitant produce of cross-linked myosin heavy chain with large molecular sizes. It was thus proposed that the cross-likning of myosin in salted fish paste is responsible for textural changes of the gels.抄録全体を表示PDF形式でダウンロード (2171K) -
尾上 義夫, 野澤 洽治, 九万田 一巳, 武田 健二, 荒牧 孝行1985 年 51 巻 9 号 p. 1567
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (898K) -
小林 博, 石田 義成1985 年 51 巻 9 号 p. 1569
発行日: 1985/09/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (92K)
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