NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 52, Issue 11
Displaying 1-27 of 27 articles from this issue
  • Makoto Nagoshi, Hiroyuki Kurita
    1986 Volume 52 Issue 11 Pages 1875-1879
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In order to clarify the relationship between population density and production of the age 0 group of the redspot masu-trout Oncorhynchus rhodurus in the upper stream of the river Kumozu, population density, growth and production were monthly examined from 1977 to 1982. The population density and the growths in body length and body weight, markedly fluctuated from year to year. Negative correlation was recognized between growth and population density. The relationship between production and population density could be represented by a dome-shaped curve. The growth and production of the age 0 group was density-dependent in the first four months of life. It was considered that some intraspecific competition originating increase of population density is the most important factor affecting growth and production.
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  • Katsuaki Nashimoto, Satoshi Motoya, Masafumi Uchida, Hitoshi Ishiguro
    1986 Volume 52 Issue 11 Pages 1881-1885
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    An attempt was made to estimate the catch efficiency of the great northern tellin hydraulic jet dredges that are being used in the Shiriuchi sea area. In the dredging survey, 2 dredges, one towed behind the other at a distance of 2 meters apart, were used simultaneously in the same fishing ground. The number of shells caught by each dredge were counted and their lengths were measured.
    Under the condition of this dredging survey, the catch efficiency is considered to be a function of the ratio of the number of shells caught by the dredge in the first position to the number of those caught behind it, using shells of the same length.
    In this way, the fishing performance for hydraulic jet dredge was evaluated. Mean relative catching effciency due to fishing selectivity of dredge was 0.82, and coefficient proportion to catch-ing rate was 0.68. The average catch efficiency was 0.56 which was estimated from these values.
    It was found that the catch efficiency of the hydraulic jet dredge for great northern tellin is higher than the common dredge for ark shell and Japanese surf clams.
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  • Heisuke Nakagawa, Shogoro Kasahara
    1986 Volume 52 Issue 11 Pages 1887-1893
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    A diet supplemented with 5% Ulva pertusa meal was fed to red sea bream Pagrus major. Growth was not affected, but hepatosomatic index and intraperitoneal fat body (IPF) ratio de-creased slightly. Plasma nonesterified fatty acids (NEFA) and amino nitrogen changed signi-ficantly. The lipid levels of the dorsal muscle and liver were not affected apparently but the lipid class composition in the liver changed remarkably. Highly unsaturated fatty acids accumulated selectively in triglycerides of the Ulva meal fed fish. As to similarity of fatty acid composition, triglycerides of the liver and IPF were low between the two dietary groups.
    Fish were subsequently starved for 21 days. Increase in plasma NEFA and decrease in amino nitrogen found in the experimental group suggested that the Ulva meal activated lipid mobilization and suppressed protein breakdown.
    Starvation resulted in the utilization of highly unsaturated fatty acids in the Ulva meal fed fish, but fatty acids of the control group were uniformly consumed. Ulva meal was demonstrated to be capable of regulating lipid accumulation and mobilization in red sea bream.
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  • Yuzuru Suzuki
    1986 Volume 52 Issue 11 Pages 1895-1899
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    Basophil granulocytes of carp and puffer were studied cytochemically by light microscope. The basoghil cells were round with an eccentric nucleus and many coarse granules. These granules were found to be extremely water soluble, aud fixed with basic dyes or fourth amines. They were stained by basophilic procedures and showed metachromasia with basic dyes. These findings indicate that the granules contain acid mucopolysaccharide, and“basophil”is a rational name for the granulocyte in fishes. However, these granules did not stain by astra blue or alcian blue procedures. Furthermore, metachromasia was not seen at pH 1.5 in puffer, and 3.0 in carp. In addition, basophilic material combined with basic dye was extruded easily even in extremely diluted electrolyte solution. These results indicate that the rnucapolysacchazide in fishes does not contain many suffate radicals in the molecule. This indication is in contrast to heparin in mam-malian basophils.
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  • Kazuo Tabata, Shigeaki Gorie, Kazuhiko Nakamura
    1986 Volume 52 Issue 11 Pages 1901-1904
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    The following results were obtained from the experiments toind optimum conditions forinduction of gynogenetic diploids in hirame Paralichthys olivaceus.
    The Ringer solutions for Pleuronectes and freshwater fish seem to be adequate as the diluting solution for milt of hirame because spermatozoa aren't motile in either Ringer solution and resisted for at least 3 hours when they were stored at 3°C.
    Keeping temperature constant during irradiation is required because the intensity of UV depended on the temperature.
    The hatching rate of eggs inseminated with milt irradiated by UV rays was almost 0% at the dosage of 110-230 erg/mn2, but it recovered when milt was irradiated with more than 660 erg/mm2 and all frys showed to haploid syndrome(n=24).
    Gynogenetic diploids of hirame were induced by cold shock treatment-put the fertilised eggsin sea water of0°C for 45-60 min from 2-5 min after insemination with milt irradiated by UV rays at the dosage of 660-1, 380 erg/mm2. Heat shocks were not effective for induction of gynogeneticdiPloid.
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  • Katsutaro Yamamoto
    1986 Volume 52 Issue 11 Pages 1905-1911
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    This paper attempts to define a mathematical expression for the strand and yarn paths in a braided rope. The theoretical development is based on three assumptions as follows:
    The projectional path of the center of a strand on a cross-sectional plane to the rope axis is an ellipse, namely the strand axis laid on the surface of an elliptic cylinder.
    The relation between the length of a strand axis, ls, and the corresponding eccentric angle of the ellipse, t, is given as dsl/dt=c(constant).
    The relation between the rotated angle of a yarn around the strand axis, φ, and the eccentric angle t is written as dφ/dt=ρ(constant).
    Under the above assumptions, the positions of strand and yarn in the coordinate system with its center in the rope axis are introduced as mathematical expressions.
    To experimentally confirm the applicability of the expressions to an actual rope, the positions of the strand and yarn in a thin cross section of a commercial polyethylene 8-strand braided rope solidified by a polyester resin were observed using a profile projector. The results of the observed positions are in good agreement with the calculated ones.
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  • Takashi Aoki, Atushi Takahashi
    1986 Volume 52 Issue 11 Pages 1913-1917
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    The non-transferable drug resistance plasmid pJA8102-2 encoded with tetracycline resistance was detected in the bacterial fish pathogen Aeromonas salmonicida. Escherichia coli carrying pJA8102-2 showed moderate resistance to tetracycline and low level resistance to minocycline. In addition, that carrying pBR322 derived from a tetracycline (tet) resistant gene of the Salmonella plasmid pSC1011) had the same resistance. The oxytetracycline resistance level cou1d not be induced after the E. coli carrying pJA8102-2 was exposed for 30 min to a culture containing a subinhibitory concentration of 1μg/ml of oxytetracycline. However, the oxytetracycline resistance level was induced by exposing it for 18h. The tet resistant gene of this 11.4 kilo base drug re-sistance plasmid was found to be located on a transposon. Restriction enzyme analysis and hybridization experiments indicated close relationship to the tet gene of pBR322. The proteins produced by this tet gene had molecular weights of 16.5, 17.5, 26, and 34 Kilo daltons (Kd). The 26 and 34 Kd of these proteins were similar to those produced by pSC101.2)
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  • E. B. C. Ufodike, C. T. Madu
    1986 Volume 52 Issue 11 Pages 1919-1922
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    Three groups of Sarotherodon niloticus fry were fed for 8 weeks on diets either treated with 17α-methyltestosterone (MT), alcohol (CA), or untreated (CO). Growth rate and food utilization in the different groups were compared. Results indicate that the best growth, Food Conversion Ratio (FCR), Protein Efficiency Ratio (PER) and Mean Growth Rate (MGR) were obtained with the MT diet. There was no significant difference (P>0.05) in growth and food utilization of the CA and CO fry, nor in the mortality rate of the three treatments. The androgen methyltestosterone promotes growth and protein anabolism without producing toxic effects in S. niloticus.
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  • Toyoji Kaneko, Katsumi Aida, Isao Hanyu
    1986 Volume 52 Issue 11 Pages 1923-1928
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    Repeated spawnings of the chichibu-goby Tridentiger obscurus and changes in ovarian activity and fine structure of the pituitary gonadotrophs during the spawning cycle were studied.
    The goby repeated spawning from 2 to 10 times in the experimental tanks. Although the spawning intervals were variable, they spawned mostly at intervals of about 10 days. Just after spawning (Day 0) GSI decreased to 2.0-3.5% and began to increase by Day 3. Afterward the oocytes grew rapidly with a sharp increase in GSI, while plasma E2 level kept high.
    In the pituitary gland three stages (II, III and IV) of gonadotrophs were observed during the cycle. Stage II cells contained numerous secretory granules, and Stage IV was characterized by the presence of cisternal rough endoplasmic reticulum. Stage II was considered to be a transient, intermediate stage between Stage II and IV. Our observations suggest that the preovulatory GTH surge results mainly from the transformation of the gonadotrophs from Stage II to IV via Stage III.
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  • Etsuro Yamaha, Kazuya Usui, Hiroshi Onozato, Keikichi Hamada
    1986 Volume 52 Issue 11 Pages 1929-1934
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    A new dechorionatiog solution for goldfish eggs was developed. A physiological saline solu-tion for freshwater fish containing 0.1% trypsin and 0.4% urea and adjusted to pH 7 was found perfect for dechorionation. Eggs were denuded completely within ten minutes in the solution and some of them developed to the hatching stage in the sterile Ringer's solution. Elastodisk connec-tion was sometimes observed at the 16-32 cell stage.
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  • Osame Tabeta, Yoshinobu Konishi
    1986 Volume 52 Issue 11 Pages 1935-1937
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    A leptocephalus larva (58mm SL) of Anguilla japonica Temminek et Schlegel was collected with IKMT of 6 feet type having 3mm mesh size in a depth of less than 200m over a bottom depth of about 1, 000m at 24°06.2'N, 125°46.8'E, about 40 miles south of Miyako Island, Okinawa, on December 4, 1978, 19:55-20:05 hours. Identification was confirmed from the following char-acters: total myomere 117, predorsal 66, preanal 75, ano-dorsal 8, first vertical blood vessei at 15th myomere and last vertical blood vessel at 44th myomere. The present larva was the 57th specimen of A. japonica, and the shallowest and the northernmost recorded. The body length, and season, time of day, water layer and water temperature of occurrence of the present larva were similar to those of larvae collected previously. The main habitat of the Japanese eel larvae seems to be located in more southern waters than the present surveyed area.
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  • Motoi Yoshioka, Etsuko Mohri, Teruo Tobayama, Katsumi Aida, Isao Hanyu
    1986 Volume 52 Issue 11 Pages 1939-1946
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    To describe the changes in reproductive hormone levels in the bottle-nosed dolphin Tursiops truncatus gilli, serum samples were obtained at 5-7 day intervals or biweekly from three sexually mature females kept in captivity wlthout adult males for period of 13 or 31 months from June 1982 through December 1984. Serum levels of estradiol, progesterone and LH were measured by radioimmunoassay (RIA). In spring through fall, 2 to 7 cyclic rises of progesterone, some of which were preceded by estradiol elevations, occurred at about one month intervals. No cyclic rise of progesterone was found in winter. A markedly elevated level of LH assumed to be a pre-ovulatory surge was detected. These changes in serum hormone levels were considered to represent sexual cycles with ovulations occurring spontaneously in the species. One female, however, showed prolonged luteal phase lasting about 5 months twice for three years, which was taken to be indicative of false pregnancy. Another female exhibited no elevation of progesterone levels through a one year period.
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  • Arao Tsuruta, Syunshiroh Ueno, Masaharu Ohgai, Machiko Yamada
    1986 Volume 52 Issue 11 Pages 1947-1955
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    The seasonal and horizontal distributions of phytoplankton communities in Yatsushiro Sea, Kumamoto and Kagoshima Prefectures, were investigated by means of a cluster analysis.
    Neritic diatoms and dinoflagellates dominate the phytoplankton communities. The structure and the distribution are largely different between the northern and the southern regions. In the northern region, a single, wide-spread community with higher cell density and lower diversity is observed, while in the southern region a few, smaller communites with lower cell density and higher diversity are distributed. Dinoflagellate-dominated communities are found in the southern region only in May and August.
    The distribution of communities, more or less, correlates with depth, degree of inshoreness and salinity. The higher values of degree of inshoreness are obtained in the northern region. There the salinity decreases to about 30‰ due to the effect of the inflowing river water. In the southern region, on the other hand, degree of inshoreness is less than 10 and salinity is more than 32.5‰, due to the effect of the inflowing offshore water through Nagashima Straight. These dif-ferences of the hydrographic condition between the northern and the southern regions are con-sidered to affect remarkably the structure and the distribution of phytoplankton communities in Yatsushiro Sea.
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  • Koichi Okuzawa, Kiyoshi Furukawa, Katsumi Aida, Isao Hanyu
    1986 Volume 52 Issue 11 Pages 1957-1960
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Honmoroko Gnathopogon elongatus caerulescens were captured from Lake Biwa in October and reared under natural conditions for up to 18 months. Fish were sampled every one or two months and seasonal changes of gonadsomatic indices (GSI), plasma levels and pituitary contentof gonadotropin (GtH) of both sexes, plasma levels of estradiol-17β (E2) of females and plasma levels of testosterone of males were examined. Histological changes of gonads were also examined, Gametogenesis of honmoroko started in October and spawning was observed from mid Aprilto late July. GSI gradually increased from October and reached a maximum in April. Changes in the plasma steroid hormones correlated well with those of GSI. These steroids are considered to be involved in vitellogenesis and spermatogenesis in this subspecies. Plasma GtH levels in both sexes showed correlation with water temperature being elevated at high water temperatures. Pituitary GtH content was high in winter, and inverse correlation between pituitary content and plasma levels of GtH was indicated.
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  • Katsuhiko Harada
    1986 Volume 52 Issue 11 Pages 1961-1968
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The feeding attraction activities as single and as combination of twenty-six nucleic acid-related compounds were behaviorally and statistically investigated on young abalone Haliotis discus, adult oriental weatherfish Misqurunus anguillicaudatus and juvenile yellowtail Seriola quinqueradiata. As a single compound, the activities for the abalone, the oriental weatherfish and the yellowtail were high in cytosine, xanthine and adenosine-5'-monophosphate (AMP), adenine, guanosine and guanine-5'-monophosphate (GMP), and adenine, cytosine, guanine, AMP, 2'-deoxyguanine-5'-monophosphate (dGMP) and uridine-5'-monophosphate (UMP), respectively. In this context, it was clarified that pyrimidine and purine bases, and nucleoside in addition to nucleotides were potent chemoattractants although the effective compounds have a wide difference among the test animals. Among the combination of the effective compounds, the activities for all the test animals became higher in the order, two, three and/or four combination. Such a finding showed that the test animals were strongly attracted by the collaborative effect rather than the single one.
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  • Harumi Tozawa, Toshiharu Kawabata
    1986 Volume 52 Issue 11 Pages 1969-1974
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Volatile N-nitrosamines (VNA) in 32 commercial fish meal samples were analyzed by a gas chromatograph equipped with a thermal energy analyzer (GC-TEA), and three different VNA, N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR) and N-nitrosothiazolidine (NTHZ) were detected in these samples in common.
    The VNA levels were apparently varied depending on the method of preparation, particularly on the drying system of meals. The VNA levels in fish meals which had been dried by blowing hot air exhausted from an oil burner (direct heating-drying type) were found to be much higher than those dried by a steam heating drier system (indirect drying type). The reason for high levels of VNA in the meals prepared by direct heating-drying system might be associated with the higher NOX concentration in the heating air.
    As to the components of VNA in fish meal samples, the quantities of NDMA were the highest, followed by those of NPYR and NTHZ. The NDMA contents in the direct heating-drying type of fish meals were fairly high, and the maximum value was as much as 1400μg/kg. The mean value of NDMA in this type of meals was about 40times higher than that of NPYR, and 80times that of NTHZ.
    It was noted that carcinogenic N-nitrosomorpholine (NMOR) could he detected in two samples, although the migration route of morpholine, the precursor of NMOR, into these samples remained unclarified.
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  • Michiyo Murata, Morihiko Sakaguchi
    1986 Volume 52 Issue 11 Pages 1975-1980
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    The contents of free amino acids (FAA) in the whole body and adductor muscle of oyster Crassostrea gigas, which had been stored in ice until they reached early stage of spoilage, were measured. Trimethylamine (TMA), and nonprotein nitrogen (NP-N) together with volatile base nitrogen (VB-N) and trimethylamine oxide (TMAO), were also determined. The content of VB-N at 0day was relatively low (10.5 and 8.7mg /100g) and increased significantly thereafter in both the whole body and adductor muscle during storage for 2weeks. Only a little TMAO was detected and TMA was about 5 mg/100g; there was almost no change in the contents during storage in either the whole body or adductor muscle. NP-N was 485mg/100g in the whole body and 515mg/100g in the adductor muscle at 0day, and no measurahle change thereafter. In fresh whole body, the level of taurine was extremely high (974mg/100mg) and the amounts of glycine, proline, glutamic acid, and alanine were relatively large. Taurine and glycine changed little throughout the storage period. Alanine showed a slight increase, while proline and glutamic acid decreased. In fresh adductor muscle, the level of taurine was also high (768mg/100g) and the levels of glycine, β-alanine, arginine, alanine, and proline were relatively high. Taurine and glycine exhibited little change during storage. Remarkable increase in alanine and decrease in glutamic acid levels were observed.
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  • Kunio Suetsuna, Katsuhiro Osajima
    1986 Volume 52 Issue 11 Pages 1981-1984
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
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    In order to isolate basic peptides having the inhibitory activities against Angiotensin I-con-verting enzyme (ACE) we investigated the enzymatically hydrolyzed products of sardine and hair tail meat. The concentration of purified basic peptides providing 50% inhibition was 39.7mg/100ml for sardine and 90.1mg/100ml for hair tail. Molecular weights of the basic peptides from both species by high performance liquid chromatography were 1000-2000. Amino acid analysis showed that the basic peptides contained such a large quantity of lysine as 47.68g/100g for sardine and 59.65g/100g for hair tail.
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  • Takeshi Umezu, Yôko Minamisako, Kenji Tabata
    1986 Volume 52 Issue 11 Pages 1985-1993
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Specific radioactivities (SR) of 60Co/50Co 137Cs/133Cs, 90Sr/86-8Sr, the elemental abundance of of K, Rb, Fe, Zn, Ag and 14 others were determined on Beryx splendens collected from the southwest coast of the Africa on the Atlantic and at the Emperor Seamounts (E-smt) on the western North Pacific. An overall ratio of 137Cs 90Sr and their SR was 1:1/4:1/8 in Beryx from Kinmei (K-smt) of E-smt, Valdivian smt (V-smt) on Walvis Ridge and A-smt on Guinea Rise. Muscle content of 137Cs was the highest: 0.85Bq/kg wet weight in K-smt Beryx and the lowest: 0.12Bq in A-smt Beryx; this reflects well 137Cs content in seawater. By the concentration Factor 1.48× 102 (133Cs muscle/133 Cs seawater) and 137 Cs muscle, content of 137Cs in Beryx habitat was estimated at 6-7mBq/l for K-smt, 1.5-2.0 mBq for V-smt and 0.7-1.0 mBq for A-smt. The strong differences were, first attributed to the different fallout by latitudes. 60Co ranging 30-150 mBq/kg wet weight was detected in livers. of Beryx and a similar level of 60Co was found in the pectoral dark muscle of Beryx exclusively from the Pacific specimens (K-smt, Sagami Bay, ShichitÔ-IÔjima Ridge, Nanse-shotÔ Ridge, KyÛshÛ-Palau Ridge). Coefficient of variation (CV) of radionuclides and their SR were in 60-100% among A-V-K-smt Beryx. However, CV ranging within 10-34% in the Pacific 6 groups. In most cases, CV of many elements by each part lay less than 45% throagh the three localities, except Ag and Cl. Thus, the benthopelagic Beryx splendens may serve as an indicator reflecting environmental radioactivities in 200-600m depth of the Oceans.
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  • Yutaka Kobayashi, Takayuki Nakagawa, Fumio Nagayama
    1986 Volume 52 Issue 11 Pages 1995-1999
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Guanidino compounds containing agmatine (AG) in the herbivorous gastropoda such as topshell Batillus cornutus and abalone Haliotis discus hannai, and in 25 species of the marine algae from 11 sampling places were analyzed in high-performance liquid chromatography (HPLC).
    In the visceras of gastropoda from 11 sampling places, AG was detected in a wide range of 1.14-13.2mg/100g tissue in topshell and 0.1-33.8mg/100g tissue in abalone. AG was also found in the topshell muscle in the range between 0.71 and 2.65mg/100g tissue, on the other hand, no appreciable amount of AG was detected in the abalone muscle from any sampling place.
    Many marine algae, natural foods for the herbivorous gastropoda, contained a detectable amount of AG.
    In the tissues of carnivorous gastropoda such as Muricidae and Buccinidae and in the squid muscle, no detectable amount of AG was found. Moreover, in our preliminary experiments, azginine decarboxylase activities could not be detected in the topshell tissues.
    From the above results, the origin of AG in molluscan tissues is considered to be algae.
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  • Keiko Hatae, Akiko Tobimatsu, Mayumi Takeyama, Juichiro J. Matsumoto
    1986 Volume 52 Issue 11 Pages 2001-2007
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Contribution of the connective tissue content on the meat texture of fish species was studied. Firmness of texturometer tests and collagen content of the raw and cooked dorsal muscles of five fish species were determined. Five species selected were skipjack, flying fish, common horse mackerel, plaice and channel rock fish. The firmness value of raw meat varied in a decreasing order: plaice, channel rock fish, flying fish, skipjack, and common horse mackerel. That of cooked meat varied in a decreasing order: skipjack, flying fish, common horse mackerel, plaiceand channel rock fish. The order of the above series was in reverse between raw meat and cooked meat. The species with softer raw meat textures, on cooking gave a firmer texture.
    The amount of connective tissues as expressed by collagen content was determined on the muscle portion as the same with the texture measurements. Higher the collagen content, firmer the raw meat. There was a significant correlation (r: 0.697) between the collagen content and the firmness. The species with firmer raw meat texture contained higher collagen content than the species with softer texture. The amount of water soluble collagen at 20°C pH 7.0, assumed as an index of the exuding collagen during mustication, increased with the decrease of the firmness of raw meat (r: 0.650). The amount of water insoluble collagen gave higher correlation coe-fficient(r: 0.744) with the firmness. These results suggest that the properties and/or composition of collagen is varied by spies.
    However, between the cooked meat and the collagen content, such relationships was hardly recognized, and this was attributed to the collagen which is solubilized during cooking. There was no appreciable correlation between the amount of water soluble collagen at 70°C and the fumness of cooked meat, though cooked beef muscle has been reported to give such correlation.
    The connective tissue proteins must contribute to the raw meat texture, while the cooked meat texture does not depend upon the connective tissue proteins, but, probably on the other factors, like the muscle fiber characteristics as reported before.
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  • Tomoko Kojima, Mamoru Sato, Reiji Yoshinaka, Shizunori Ikeda
    1986 Volume 52 Issue 11 Pages 2009-2017
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The proximate composition of various tissues and the fatty acid composition of their lipids in several fishes except Cyprinidae in Lake Biwa were determined in order to evaluate the nutritive value of fresh water fish. The fishes examined were namazu Silurus asotus, nijimasu Salmo gairdneri, biwamasu Oncorhynchus masou rhodurus, ayu Plecoglossus altivelis, ohkuchibasu Micropterus salmoides, bluegill Lepomrs macrochirus, unagi Anguilla japonica, yoshinoboti Rhino- gobius brunneus, and isaza Chaenogobius isaza.
    The results obtained were as follows.
    (1) The contents of moisture, crude ash, crude protein and crude lipid in edible portions were 68.3-84.6, 1.0-3.1, 12.1-22.5 and 1.2-16.2%, respectively. The lipid contents in the edible portions of biwamasu and unagi were much higher than those in the edible portions of other fishes,
    (2) Lipids of the edible portions were rich in palmitic acid (C16:0, ), palmitoleic acid (C16:1ω9)and oleic acid(C18=1ω9).
    (3) The content of total polyunsaturated fatty acid was lower in lipids of the muscles or the whole bodies of fresh water fishes than in those of the muscles of marine fishes.
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  • Yutaka Tateda, Taku Koyanagi
    1986 Volume 52 Issue 11 Pages 2019-2026
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The accumulation of 60Co, 59Fe, 54Mn and 65Zn by common mussel Mytilus edulis and purplish bifurcate mussel Septifer virgatus from seawater was investigated. High accumulation of 59Fe in Mytilus and 54Mn in Septifer was observed in the uptake experiments. Differences in biological half-lives between the two species was observed for 54Mn and 59Fe. The organ-specific concen-tration of radionuclides observed in the mussels was high accumulation of 60Co in kidney andviscera, 59Fe in kidney and viscera, 65Zn in kidney but was not observed for 54Mn although the accumulation of 54Mn in mantle was different between the species. Autoradiography was applied to examine the details of the distribution of 59Fe in the soft parts of the mussels.
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  • Yutaka Shimizu, Akira Nomura, Fujio Nishioka
    1986 Volume 52 Issue 11 Pages 2027-2032
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Myosin preparations different in the madori-susceptibility were applied to Sepharose 6B gel filtration (0.6M KCl, pH 7.2), and a minor fraction of M. W. 104-1.6×105 was found in large quantities in the modori-susceptible preparations from croaker, filefish and chub-mackerel as compared with the modori-stablc preparation from grub fish. In the major fraction separated from croaker myosin preparation, the modori phenomenon did not occur by itself, while the minor fraction induced a remarkable degradation to the modori-stable flying fish myofibrills at 60°C. The modori-inducing property of croaker ordinary muscle appeared to be rarely distributed in the sarcoplasmic fraction, but unevenly in the myofibrillar, especially myosin fraction.
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  • Ikushi Matsumoto, Ken-ichi Arai
    1986 Volume 52 Issue 11 Pages 2033-2038
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The protective effect of several kinds of sugars on freeze denaturation of carp myofibrillar protein was studied by measuring the rate constant (kD) for inactivation of its Ca-ATPase.
    As the inactivation of Ca-ATPase proceeded in two first order steps, the rate of an early fast step was employed to make a linear relation as a function of sugar concentration (M). The pro-tective effect of sugar on freeze denaturation of myofibrillar protein was estimated from the slope of log kD versus sugar (M). The protective effect (E) was thus found to become lower in the order of lactitol, glucose, maltitol=sucrose, sorbitol, and maltose. This rank of sugars coincided with that of the same sugars against thermal denaturation of fish myofibrillar protein, which has been previously reported.
    It was also found that a relatively lower concentration (0.1-0.3M) of sugar was enough to protect freeze denaturation of myofibriliar protein than that of sugar required to protect thermal denaturation of the same protein.
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  • Toshio Akiyama, Katsuyoshi Mori, Takeshi Murai
    1986 Volume 52 Issue 11 Pages 2039
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (89K)
  • Nobuaki Takahashi
    1986 Volume 52 Issue 11 Pages 2041
    Published: November 25, 1986
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (101K)
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