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Volume 52, Issue 12
Displaying 1-26 of 26 articles from this issue
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Jotarou Urabe, Takashi Maruyama1986 Volume 52 Issue 12 Pages 2045-2054
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to evaluate the predational impact on various zooplankton species, gut contents were examined on two dominant cyprinid fishes, Honmoroko Gnathopogon elongatus caerulescens and Oikawa Zacco platypus, in Ogochi Reservoir through warm water season. Crustacean plankton is usually dominant in the gut contents of both fishes and one of the largest crustacean prey species Daphnia galeata is exclusively dominant when this species is abundant. When Daphnia is scarce, Honmoroko preys mainly on other crustacean plankton such as copepodids of Cyclopidae and Diaphanosoma, but total ingestion is relatively low regardless of the abundance of these crustacean plankton. On the other hand, Oikawa preys mainly on terrestrial insects. The amount of in-gestion for some other zooplankton species such as rotifers, Bosmina and Bosminopsis is con-siderably small in both fish species. These results suggest that the most vulnerable zooplankton to fish predation is Daphnia in this reservoir and other zooplankton may have more chance to coexist with Honmoroko and Oikawa because of the small size or escape ability.View full abstractDownload PDF (586K) -
Yayoi Ikeda, Jiro Koyama, Hisao Ozaki1986 Volume 52 Issue 12 Pages 2055-2059
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (363K) -
Hossein Razani, Isao Hanyu1986 Volume 52 Issue 12 Pages 2061-2067
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOvazies of both yearling and 2-3 year old goldfish remained suppressed indefinitely at 24°C/12L. The older fish were unresponsive when transferred to 24°C/16L, while some yearling females advanced to the yolk globule stage and a few spawned at 24°C/16L or 16°C/16L. Most yearling females started maturation when transferred to natural conditions on Jan. 5. The ovarian matura-tion was further accelerated when the females were exposed to 24 or 16°C combined with 12 or 16L following 2 months stay under natural conditions.
On the other hand, both ages of males matured at 24°C/12L. Spermatogenesis was accelerated when transferred to 24°C/16L. However, on both regimes the males passed into post-spawning within a short period. Although spermatogenesis slowed down in fish transferred to 16°C/16L, the fish kept active testicular activity after full maturation. Spermatogenesis became even slower in the fish kept under natural conditions of winter, but it was accelerated when fish were transferred to 24 or 16°C.View full abstractDownload PDF (516K) -
Ichiro Aoki, Tadashi Inagaki, Le Van Long1986 Volume 52 Issue 12 Pages 2069-2077
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe three-dimensional structure of pelagic fish schools was investigated at sea during the night by means of underwater stereo-photography. Jack mackerel Trachurus japonicus and mackerel Scomber sp. were photographed. At night, compact and polarized schools were main-tained and, on the other hand, some fish were dispersed or aggregated in very small schools. Es-timated body lengths ranged from 13 to 31cm with an average of 20cm for a school of jack mackerel and 14 to 23cm with an average of l6cm for a school of mackerel. Within the school, jack mackerel of similar size tended to swim next to each other. The mean distance to nearest neighbour was 1.43 body lengths for the jack mackerel school and 1.51 body lengths for the mackerel school. In the vertical plane, jack mackerel seem to swim at the same level as their nearest neighbours at one moment and take up diagonal positions to them at another. In the horizontal plane, jack mackerel less frequently have nearest neighbours at the side. The density estimated for the jack mackerel school ranged from 6.6 to 19.5 fish/m3.View full abstractDownload PDF (1342K) -
Izuru Kakuta, Kenji Nanba, Kazumasa Uematsu, Shiro Murachi1986 Volume 52 Issue 12 Pages 2079-2089
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSUrine properties of carp in summer season were examined at regular intervals for 3 days. As listed below, each property changed incessantly but within a constant range, even under the near-natural conditions for fish (no restriction in water) used in this study. Urine flow 8.37±1.57ml/kg body Weight/h; osmolarity 29.4±1.2 mOsm/l; pH 7.23±0.06; inorganic ion con-centrations (mEq/l), Na+ 12.05±0.68, K+ 1.40±0.28, Ca2+ 0.54±0.22, Mg2+0.23±0.06 and; nitrogen compound concentrations (mg-N/dl), ammonium 10.71±1.77, urea 0.72±0.11, uric acid 0.57±0.01, creative 9.0±0.4, creatinine 1.0±0.3 and protein 1.36±0.36 (each of these data was expressed as the average value±standard deviation, using 5 test fishes).View full abstractDownload PDF (650K) -
Osamu Fukuhara, Tohru Nakagawa, Tatsuhiro Fukunaga1986 Volume 52 Issue 12 Pages 2091-2098
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSMorphological development of early life stages in laboratory-reared yellowtail was observed and reported with special reference to fin development, pigmentation and squamation. The specimens were obtained serially by experimental rearing from eggs to larvae, measuring 3.5mm in standard length (SL) at hatching and to young (about 70mm SL). Larval development and squamation were illustrated. The transition from post-larvae to juveniles occurred between 9.85 to 13.5mm SL. Squamation began in larvae 22.1mm SL and was completed when thelarvae attained 40.2mm SL. Larval growth during initial 40 days was expressed by the equation SL=3.27e0.048x, where SL=standard length, and X=days after hatching. Some implications in morphological development of external and internal characters was discussed for better under-standing the early life stages of Seriola quinqueradiata.View full abstractDownload PDF (711K) -
Osame Tabeta, Yoshie Dotsu, Michihisa Abe1986 Volume 52 Issue 12 Pages 2099-2102
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOver 500 samples of the toxic swellfish Lagocephalus lunaris (BLOCH et SCHNEIDER) were taken with pair trawlers and large-and medium-sized purse seiners in the shallower waters less than 200m depth south of 32°N in the East China Sea in 1980-1985. Examination of the gonads indicated that the spawning season was in the early summer in the southern and continental side of the Sea and the spawning fish were more than two years old. The fish reached about 18cm BL at the end of the year, about 25cm in one year and about 30cm BL in two years from their birth based on their size frequencies. Swellfish less than 20cm BL formed more than 90% of the total catch with the pair trawlers and concentrated in the central area of the Sea between Octo-ber and December in 1983. The swellfisb were inferred not to be schooling in the fishlng ground. The catch with pair trawlers varied from year to year; the prominent occurrence, which was less than 1 ton, was in April 1983-March 1984. The location of capture of the sewllfish L. lunaris differed mazkedly from the fishing ground of the swellfish L. gloveri which is the main catchable stock of the genus Lagocephalus in the Sea.View full abstractDownload PDF (340K) -
Osame Tabeta, Yoshie Dotsu, Michihisa Abe1986 Volume 52 Issue 12 Pages 2103-2105
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFive cases of occurrence of the toxic swellfish Lagocephalus lunaris (BLOCH et SCHNEIDER) have been recorded from the coastal waters of Japan: two specimens from Fukagawa Bay, Yamaguchi Pref., Japan Sea, in November 1977; one from Lake Hamana, Shizuoka Pref., on November 4, 1983; one from the coastal water of Nawa-machi, Tottori Pref., on June 28, 1984; tens from the waters 9.5 miles northwest of Ikitsuki L, Nagasaki Pref., in September 1984; one from offshore Kushikino, Kagoshima Pref., on December 4, 1985. Previously, the toxic swellfish was only known to occur in the waters to the south and west of Japan. The fish described above were young and adult fish (21-41cm TL). These records extend the range of this toxic swellfish into the coastal waters of middle Japan. More specimens were recorded from the coastal waters of the East China Sea-Japan Sea side than on the Pacific coasts. Rare cases of food poisoning have been attributed to Lagocephalus spp. in Okinawa-Mie Prefectures of the Pacific side, and Naga-saki, Shimane and Fukui Prefectures of the East China Sea-Japan Sea side. It is concluded that the swellfish Lagocephalus lunaris may be responsible for these cases of food poisoning.View full abstractDownload PDF (454K) -
Mitsumasa Mankura, Mitsu Kayama, Noriaki Iijima1986 Volume 52 Issue 12 Pages 2107-2114
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe hydrolysis of L-1-palmitoyl-2- [1-14C] arachidonyl-3-sn-glycerophosphatidylcholine (14C-PC) was studied with carp hepatopancreas preparation. Phospholipase A2 of 10, 000×g supernatant showed the highest activity around 35 to 45°C at pH 5.0. The enzyme was activated by the addition of Ca2+ (10mM), sodium deoxycholate (5.0mM) and Triton X-100 (10mM) to the reaction mixture, whereas ethylenediaminetetraacetic acid (EDTA, 0.5mM) did not stimulate the activity. Some parts of labelled acid released from 14C-PC were incorporated into triglycerides, partial glycerides, and wax esters. The incorporation of radioactivity into wax esters was promoted by the addition of oleyl alcohol. Both the hydrolyzing activity of 14C-PC and the synthesizing activity of wax ester from 14C-PC were distributed in 700×g pellet, lipid cap, 8, 000×g pellet (mitochondrial fraction), 105, 000×g pellet (microsomal fraction), and 105, 000×g pellet supernatant. Moreover, the role of phospholipase A2 on wax ester synthesis in carp hepatopancreas preparation was discussed.View full abstractDownload PDF (610K) -
Norihisa Nishi, Hirotada Ozaki, Michio Nonaka, Ken-ichi Arai1986 Volume 52 Issue 12 Pages 2115-2120
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe myofibrils from six species of shark were prepared and suspended in a medium of 0.16M KCl-40mM Tris buffer (pH 7.5 or 5.8) containing various concentrations (0-1.0M) of Urea. Thesuspension was then heated at a fixed temperature in the range of 25-45°C and measured for its Ca-ATPase activity with the lapse of time. The first order rate constant for inactivation wasthus calculated.
By adopting an increasing ratio of KD as a measure of accelerating effect on denaturation of myofibrillar protein, multiplying effect by the presence of Urea (0.3M), the fall in pH (from 7.5 to 5.8), and the elevation of temperature (from 25°C to 35°C) was observed for all shark myo-fibrils. Among the three factors examined, heat showed the largest effect.
From the above data thus obtained, a discussion followed to obtain favourable conditions needed for the preservation and processing of raw muscle from shark.View full abstractDownload PDF (437K) -
Eiji Niwa, Satoshi Kanoh, Teruo Nakayama1986 Volume 52 Issue 12 Pages 2121-2125
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOkada has assumed that a relation of lyotropic series is formed between various anions and their ability to cause the setting of fish fiesh sol as follows:
SCN->I->NO3-, Br->Cl->SO4-2
This was assumed by our viscoleastic measurements also here. However, the above order is entirely reverse against that of ability to enhance hydrophobic interactions among protein mo-lecules whose participation to the setting has been recently emphasized. On the other hand, it was found here that the increment of the viscosity of fish actomyosin solution during heating is enhanced by various anions in the order of citrate -3>acetate->F->Cl->Br->NO3->I->SCN-. This order is entirely reverse against the above one. Such a conflict can be solved by considering that the gel strength is determined not only by the quantity of protein network structures within gel but also by their distribution.View full abstractDownload PDF (340K) -
Eiji Niwa, Shin-ichiro Kohda, Satoshi Kanoh, Teruo Nakayama1986 Volume 52 Issue 12 Pages 2127-2130
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to clarify the reason why the white muscle fishes are more easily deteriorated byfreezing than the red muscle fishes and the warm blooded animals, the fiuorometric intensity of their myosin solutions was measured in the presence of sodium 8-anilino-1-naphthalene sulfonate. The fluorometric intensity was relatively low for the myosin solutions from the red muscle fishes, such as albacore and yellowfin tuna, and the warm blooded animals, such as pork, whale, chicken and beef, and scarcely increased on freezing overnight at -20°C. The intensity was, however, higher for the solutions from the white muscle fishes, such as angler, Alaska pollack, stone flounder, hair tail, butterfish and barracuda, and remarkably increased on freezing. Furthermore, in such the fluorometric behaviors, the pink muscle fishes, such as mullet, saury, Pacific mackerel and Spanish mackerel, showed intermediate between the red muscle fishes and the white muscle fishes. The increment of the fluorometric intensity on freezing was suppressed on the addition of sucrose and Na-glutamate.
These results suggest that in the white muscle fishes, the hydrophobic amino acid residues of myosin molecules are more easily exposed on freezing.View full abstractDownload PDF (292K) -
Kenji Takii, Sadao Shimeno, Masahiko Takeda1986 Volume 52 Issue 12 Pages 2131-2134
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe effects of a feeding stimulant mixture contalning L-alanine, glycine, L-histidine, L-proline and 5'-UMP as a dietary flavor were investigated on the activities of some hepatic enzymes relating amino acid and carbohydrate metabolisms of eel after 3 and 6h feeding on the final day of the experimental period of 25 days. At the same time the concentration of their plasma constituents were assayed.
Plasma amino acid nitrogen in the test group fed on a flavored feed was maintained at a relalively lower level than that of the control group on an unflavoured feed. Alanine aminotransferase and aspartate aminotransferase were at higher levels in the liver of the test group. Blood glucose level in the control group showed a marked increase, while it remained constant in the test group. There was a trend of increasing activities in hepatic phosphoglucose isomerase, glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase in the test group over the control group. These results suggest that the greater performance of the test group evidenced in the previous paper was indirectly attributable to the increased activities of the hepatic enzyme concerning cazbohydrate and amino acid metabolism.View full abstractDownload PDF (332K) -
Hideaki Morii, Donald C. Cann, Lesley Y. Taylor, Charles K. Murray1986 Volume 52 Issue 12 Pages 2135-2141
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe present work was carried out to investigate the role of luminous bacteria particularly Photobacterium sp. in relation to histamine formation when scombroid fish were stored at low temperatures.
The numbers of luminous bacteria on the skin of mackerel and herring did not increase during storage in ice but in the viscera they increased greatly during storage in ice and at 10°C. Moreover, the percentages of luminous bacterial counts (LBC) comprised 20% or more of the flora (TVC) during storage in ice and at 10°C as compared with 0.4% in fresh fish.
Eighty-five out of 90 strains of the luminous bacteria isolated were identified as Photobacterium phosphoreum and 5 strains of the isolates as Vibrio logei.
All the strains of P. phosphoreum isolated formed histamine with high levels in some strains but V. logei and fish spoilage bacteria comprising certain strains of Pseudomonas putida, Pseudo-monas Gp III and Moraxella sp. did not.
From these findings, it is considered that P. phosphoreum may play a significant role in hista-mine formation when scombroid fish are stored at or below temperatures of 10°C.View full abstractDownload PDF (573K) -
Wung Yang Shieh, Usio Simidu1986 Volume 52 Issue 12 Pages 2143-2147
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe abundance and structure of heterotrophic bacterial populations in an eelgrass Zostera marina field were investigated. For all the samples, the density of the aerobic bacteria was approximately tenfold higher than that of the anaerobic bacteria. Distinct differences were found between the composition of the bacterial population in rhizosphere, surrounding sediment and bottom seawater both with the aerobic and the anaerobic cultivations. The aerobic bacterial population of the bottom seawater and sediment was composed mainly of Acinetobacter-Moraxella and Flavobacrerium, whereas in eelgrass rhizosphere pseudomonads and halophilic aeromonads as well as Acinetobacter-Moraxella were dominant. Aeromonas spp. were also dominant among the anaerobic bacteria in eelgrass rhizosphere, in contrast with Vibrio spp. in the surrounding sedimentand in the bottom seawater. While the aerobic bacteria rarely showed antibacterial activity, the anaerobic bacteria, especially Aeromonas spp. from the rhizosphere, exhibited a high percentage of antibacterial activity against Staphylococcus aureus.View full abstractDownload PDF (326K) -
Yaichiro Shimma, Hisako Shimma, Kazuo Ikeda1986 Volume 52 Issue 12 Pages 2149-2154
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSHematocrit and plasma constitutents such as protein, cholesterol, phosphorus, calcium, magnesium, zinc and potassium were compared between two groups of NAGANO strain rainbow trout yearlings in August, October and December.
From May to December the control group was fed commercial diet mixed with fish oil (100:8) and the BtOH group was fed with the diet containing fish oil and n-butyl alcohol (100:8:8). Growth of the BtOH group was a little better than the control group after the first two months but became inferior to the control group later on. The final average fish weights were 322g in the control and 297g in the BtOH groups.
BtOH group showed lower value of hematocrit and cholesterol than the control group through the period. The level of protein was 4.2g/100ml in BtOH group and 3.9g/100ml in the control group in August. However, the level of BtOH group became lower than that of the control group in December.
Males of both groups matured in December and showed raised hematocrit, protein, and phosphorus but no other constituents. Relationships of protein to cholesterol and to phosphorus were discussed in the groups.View full abstractDownload PDF (356K) -
Haruka Iida, Jun-ichi Nakazoe, Hiroaki Saito, Toshio Tokunaga1986 Volume 52 Issue 12 Pages 2155-2161
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe relationship between a content of dimethyl-β-propiothetin (DMPT) as a precursor of dimethyl sulfide (DMS) in fish and DMPT levels in the fish diets was studied. Sardine, carp, and rainbow trout were fed with the diets containing low or high levels of DMPT, and then DMPT and DMS contents in fishes were determined.
DMPT contents in muscle and viscera of sardine became very low after culturing with a low level DMPT diet. On the other hand, DMPT contents in carp and rainbow trout which generally contained low level DMPT increased rapidly when fed with a high level DMPT diet.
DMPT accumulated in carp or rainbow trout decreased rapidly when the supply of DMPT had been stopped. And the maximum accumulation of DMPT in carp dorsal meat was observed when the carp had been fed with a high level DMPT diet.
The formation of DMS from DMPT in live fish was not observed, however, when fish meat aboundant in DMPT was treated at high temperature, DMPT was decomposed into DMS. On the other hand, in the case of DMPT free fish meat, an obvious amount of DMS was not formed under the same treatment. Thus it seemed that the origin of DMS was limited. to DMPT in fish meat.View full abstractDownload PDF (475K) -
Kenji Hara, Kohichi Yukawa, Tadashi Ishihara1986 Volume 52 Issue 12 Pages 2163-2169
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwo different proteins having trypsin-inhibiting activity, referred to as inhibitors FI-a and FI-b, were purified from dried purple laver Porphyra yezoensis by haet treatment, ammonium sulfate fractionation, column chromatographies on a DEAF-cellulose and CM-cellulose and gel filtration on a Sephadex G-100. The inhibitory activities of both inhibitory against bovine trypsin increased about 4, 200-fold and 4, 500-fold, respectively. The molecular weights of both inhibitors were determined to be approximately 10, 000 by gel filtration on a Sephadex G-100. Both in-hibitors were stable over a range of pH 3.0-10.5 and at 60°C for 30 min. Trypsin was strongly inhibited by both inhibitors, α-chymotrypsin and pronase were weakly inhibited, but pepsin, papain and subtilisin BPN' were not inhibited. FI-a was completely inactivated by the modifica-tion of arginine residues with 1, 2-cyclohexanedione, but was not inactivated by the modification of lysine residues with O-methylisourea. Whereas, FI-b was inactivated by the modification with O-methylisourea, but was not inactivated by 1, 2-cyclohexanedione.View full abstractDownload PDF (559K) -
Michizo Suyama, Toshiyuki Hirano, Takeshi Suzuki1986 Volume 52 Issue 12 Pages 2171-2175
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA comparison of the difference between buffering capacrities of white and dark muscles of yellowfin tuna was made using β-pH curves, and the contribution of free histidine and its related dipeptides was investigated by means of omission test of the whole synthetic extract which was prepared by blending reagent grade chemicals on the basis of analytical values of the muscle ex-tract. The homogenate and picric acid extract of the white muscle showed higher buffering capac-ity than in the dark muscle, and the curve of the whole synthetic extract of the white muscle re-sembled that of a picric acid extract. When histidine and anserine, the most prominent nitrogenous compounds in the white muscle, were omitted, the buffering capacity was almost lost, and there-fore, these constituents were found to play the most important role in the buffering of the white muscle. In the dark muscle, these constituents, naturally, did not participate in the main buffering of the muscle, since it contained relatively low amounts of histidine and anserine.View full abstractDownload PDF (349K) -
Chieko Ozawa1986 Volume 52 Issue 12 Pages 2177-2181
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAttempts were made to use sodium bicarbonate as an accelerator in detoxification of toxic puffer ovaries during pickled product processing, which is rather common in Ishikawa Prefecture, Japan.
Fresh ovaries of a puffer, “mafugu”Fugu vermicularis porphyreus were divided into three portions, sprinkled with a solid salt mixture containing O, 3.3 or 6.6% NaHCO3, and were stored for seven months, followed by one-year pickling with rice bran. Samples were taken at each step and assayed for toxicity, pH, VBN, etc.
The results obtained showed that the detoxification during the process was most marked when the solid salt containing 6.6% NaHCO3 was used for sprinkling. In addtion, organoleptic tests demonstrated that the pickled product thus processed did not differ from the one prepared according to the usual method, with respect to taste, flavor and appearance.View full abstractDownload PDF (395K) -
François Galgani, Fumio Nagayama1986 Volume 52 Issue 12 Pages 2183-2188
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe characteristics of digestive proteolysis and digestive proteinases in the crabs Portunus trituberculatus, Portunus sanguinolentus and Charybdis japonica were studied.
For these three species, a broad range of stable protease activity from pH 6-8 was found. Optimum temperature was found to be around 60°C. Proteolytic activity is stable between pH 6-8 and effects of metallic ions are similar in the three species.
Trypsin, carboxypeptidase A and leucine aminopeptidase were identified in the digestive glands of three species using synthetic substrates. However, neither chymotrypsin nor carboxy-peptidase B were found. Hydrolysis of native collagen occurs in the digestive glands of three species. In addition, after gel filtration, a proteolytic activity was observed in a low molecular weight fraction.
Optimum pH for trypsin was found around 8-8.5.View full abstractDownload PDF (361K) -
Bor-Shing Liou, Fumio Nagayama1986 Volume 52 Issue 12 Pages 2189-2195
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe pyruvate kinase(EC 2.7.1.40, PK)from carp hepatopancreas and rainbow trout liver was purified and characterized. It was assured, by chromatography on DEAE-Sephadex A-50 column, that there are two forms of PK in both fish livers. The major components of PK from carp and trout showed the maximum activities at pH 6.38 and 6.04, respectively. The molecular weight of PK from both species as estimated by gel filtration was 234, 000. Both enzymes were activated by FBP and inhibited by ATP, Ca2+ and low level of Cu2+. Several divalent cation (Mg2+or Mn2+) and monovalent cation(K+ or NH4+) were absolutely essential for their activities. Theresult of kinetic investigation suggested that the reaction of carp PK was due to “sequential” mechanism, while that of rainbow trout enzyme was due to “Ping Pong” mechanism.View full abstractDownload PDF (469K) -
Bor-Shing Liou, Fumio Nagayama1986 Volume 52 Issue 12 Pages 2197-2202
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPhosphoenolpyruvate carboxykinase (EC 4.1.1.32, PEPCK) from rainbow trout liver and carp hepatopancreas were purified and characterized kinetically. There are two forms of PEPCK in the rainbow trout liver, but only one form in the carp hepatopancreas. The molecular weight was 61, 000 for rainbow trout liver major isozyme and 59, 000 for carp hepatopancreas enzyme when determined by Sephadex G-100 gel filtration. However, when determined by SDS poly-acrylamide gel electrophoresis the former was 87, 000 and the latter was 83, 000. The optimum pH for both enzymes was 7.7 in the direction of oxalacetate (OAA) decarboxylation, and 7.2 in the direction of phosphoenolpyruvate (PEP) carboxylation. Enzyme activities depended on the concentration of substrates (OAA or PEP) and nucleotides in either direction. Both enzymes were also strongly inhibited by several nucleotides. Divalent cation was absolutly necessary for both enzymes in either direction, and Mn2+was found to be the best activator among several metal ions. Both enzymes were strongly inhibited by Zn2+, especially in the reaction of OAA decarboxylation. Co2+ and Cu2+ also showed obvious inhibition, but Ca2+ showed ambiguous effect on both enzymes. The kinetic properties of PEPCK from both fish liver were similiar not only qualitatively but also quantitatively.View full abstractDownload PDF (372K) -
Yayoi Ikeda, Jiro Koyama, Hisao Ozaki1986 Volume 52 Issue 12 Pages 2203
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (87K) -
Isolation and CharacterizationHisao Kamiya, Koji Muramoto, Takaharu Hoshino, Masatoshi Yamazaki, Uda ...1986 Volume 52 Issue 12 Pages 2205
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (119K) -
Koji Nakamura, Haruka Iida, Hiroshi Nakano1986 Volume 52 Issue 12 Pages 2207
Published: December 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (92K)
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