巻号一覧
52 巻, 5 号
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坂本 亘, 田中 祐志1986 年 52 巻 5 号 p. 767-776
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーWater temperature patterns and distributions of fish eggs and larvae in the vicinity of shallow sea front were observed at the mouth of Wakasa Bay, the Japan Sea. The front was formed between nearshore and offshore waters. The concentration per 100m3 of eggs and larvae of Japanese anchovy Engraulis japonicus in the surface layer sometimes corresponded with the direc-tion and scale of the front. The effect of the swimming activity of a larva on distribution pattern was little during the first two days after hatching out. The front shifted horizontally with tidal cycle and its pattern was affected by the longer period wave. The wave longer than tide period was caused by typhoons and low pressure.抄録全体を表示PDF形式でダウンロード (1280K) -
小川 泰樹, 角田 俊平1986 年 52 巻 5 号 p. 777-786
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーAn estimation of the population growth and life span of the Oriental river prawn Macro-brachium nipponense was carried out. 17, 541 prawns were collected in Lake Kojima, Okayama Prefecture. The montly mode of each age group by sex was obtained based on normal distribu-tion, using a computer analysis technique, from body length histogram, and plotted.
According to the monthly ratio of the number of ovigerous females to that of females of 26 mm (Biological minimum size) and larger in body length, the spawning season of the species in-habiting Lake Kojima is from Late May to the middle of September, continuing for almost four months with its peak in July
As to the mean growth of this prawn in Lake Kojima, male attains 33 mm in body length and 0.7g in body weight at one year after hatching out, 45 mm and 2.0g at two years, 61 mm and 5.1g at three years, and 63 mm and 5.6g in the perishing winter period. Female grows to 28 mm and 0.5g at one year, 47 mm and 2.3g at two years, and 57 mm and 4.1g in the perishing winter.
For male's life span 3 years and 4 to 9 months is estimated, while that of female may be 2 years and 4 to 10 months when presuming they hatch out in the middle of Juiy.
Considering the mode of the appearance of ovigerous female, it is supposed that female will mature sexually at one year after hatching out, while, judging from the developmental infection point of the second chetiped, male will mature at two years after hatching out, attaining 45 mm in body length. Consequently it is presumable that 1-year-old female and 2-year-old male will show spawning activity for the first time late in July, and male and female will experience the spawning season twice respectively within each life span抄録全体を表示PDF形式でダウンロード (682K) -
濱野 龍夫, 松浦 修平1986 年 52 巻 5 号 p. 787-794
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーExamination of the stomach contents of the Japanese mantis shrimp Oratosguilla oratoria showed that this species is a very intensive predator on the Macrura which is a principal component of the benthic megalofaunal community. Thus, the mantis shrimp, by feeding largely on the Macrura, may have a major influence on this community structure in Hakata Bay. The shrimp was also revealed to be an intensive predator on the Pelecypoda despite underestimation of preda-tion intensity, based on the stomach contents, because of its shell-smashing behaviour. Further-more, this study showed that many Macrura were captured during September and November by the mantis shrimp and that female shrimp of reproductive sizes also consume many Pelecypoda, As a prey species, mantis shrimp themselves were found infrequently in the digestive tracts of other predators.抄録全体を表示PDF形式でダウンロード (641K) -
浅野 昌充, 羽生 功1986 年 52 巻 5 号 p. 795-800
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーInvestigations were made on the behavioral response of the namazu Parasilurus asotus-nocturnal and carnivorous-to living prey (fish) or to weak electric fields generated by different sources. The results are as follows.
1. The namazu could catch prey in darkness without fail.
2. When the blinded namaau happened to come within a distance of s cm or less, it made a sudden, accurate tum to the prey and then swallowed the prey with a very swift motion.
3. The blinded namazu showed positive taxis to a weak, local electric field, and even attacked the electrodes reproducing an electric field which had been recQrded on a magnetic tape from near a prey.
4. Six blinded namazu were given choice between two cubes of carp muscle. One of the cubes was attached with electrodes reproducing the dummy electric field. They preferred the cube with electrodes to the one without (p<0.05, χ2-test).
5. Apparently, the namazu, exploring for food along the density gradient of the odor and at least getting into the azea where the electric field around the prey can be perceptible, will locate the prey accurately by means of its electric sense.抄録全体を表示PDF形式でダウンロード (766K) -
小栗 幹郎1986 年 52 巻 5 号 p. 801-804
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーYellowish brown pigment called lipofuscin is included in the adrenal gland of variousmammalian species and appears as abundant granules in the cortical cells of its zona recticularis. Among many fish species examined by the present author, the yellowish brown pigment granuleswere abundantly detected only in the interrenal cells of the anglerfish Lophius litulon and the leopardshark Triakis scyllia. For this examination, three large-sized specimens of the anglerfish wereused and their body weights were 2.5 to 8.6kg. In the leopard shark, appearance and accumu-latiop of the pigment granules were remarkable in four large-sized specimens(118-123cm in bodylength) among ten specimens(60-123cm)investigated. Accordingly, it was suggested that the yellowish brown pigment granules appeared and revealed conspicuous accumulation in the inter-renal cells of large-sized fishes with advanced age抄録全体を表示PDF形式でダウンロード (1079K) -
宗清 正廣, 桑原 昭彦1986 年 52 巻 5 号 p. 805-810
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーHorizontal and vertical distributions and transportation of the ribbon fish Trichiurus leprurus eggs and larvae were described, based upon the sampling data of multilayer horizontal tows from May to September, 1983 in the western Wakasa Bay.
The ribbon fish eggs and larvae mainly distributed from the middle to deep layers in the offshore (120min depth) from May to September. From early to late developmental stage eggs distri-buted at the same layer, and when temperature and salinity discontinuities occurred at middle layers (Jul. -Sep.), peak densities of eggs were observed from the middle to deep layers, though the eggs occurred from surface to deep layer when temperature and salinity discontinuities dis-appeared (Jun.).
Based upon the elapsed time in developments of the ribbon fish eggs and larvae and the velo-city of Tsushima Warm Current in summer, it was estimated that some of the late stage eggs and larvae of more than 7mm in total length occurred in the western Wakasa Bay were transported from far more than 40 and 60 nautical miles with axis of Tsushima Warm Current (far west of the Hyogo and Tottori offing), respectively.
From monthly changes in horizontal and vertical profiles of the eggs and larvae distributions and total length compositions of the larvae between the offshore and inshore, it was suggested that an inshore transport process of the ribbon fish eggs and larvae occurred by current trans-portation and their own positive movement.抄録全体を表示PDF形式でダウンロード (508K) -
鈴木 譲1986 年 52 巻 5 号 p. 811-816
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーFor elucidation of chemicalal mediators of the acue inflammatory reaction, leucocytic chemotactic factor in eel blood plasma was investigated by Boyden's in vitro assay system, which is to judge chemotaxis by migratory activity of leucocytes through a micropore filter to test material. The eel blood plasma which is known to have hemolytic activity, became chemoattractive for eel neutrophils when incubated with rabbit erythrocytes. This chemotactic factor did not orig-inate from the hemolysate but was produced by the reaction of the blood plasma with the cell membrane of rabbit erythrocytes. Similar chemotactic responses of neutrophils were also induced by the reaction of the blood plasma with zymosatt (cell wall of yeast), sephadex and inulin. The production of the chemoattractant was closely related with the hemolytic activity. Whereas both were decreased by heat treatment, the, individual variations of the productivity of the chemo-tactic factor correlated with that of the hemolytic activity, and the blood plasma which became chemoattractive by zymosan treatment showed no hemolytic activity. Both the hemolytic activity and the production of chemotactic factor are inferred to originate from the activation of the com-element system in the blood plasma via the alternative pathway.抄録全体を表示PDF形式でダウンロード (385K) -
田中 信彦1986 年 52 巻 5 号 p. 817-821
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーEpiphytic diatoms on the natural seaweeds (seven brown-algae, two red-algae and one green alga) were fractionated by the five step separating treatments of shaking and rubbing. The order of adhesive strength of diatom species was determined from the recovery patterns of each diatom obtained through the five step separating processes. On the basis of the order, epiphytic diatoms were grouped into four, those are, the group of the smallest adhesive strength (group I) consisting of rather planktonic diatoms and large benthic diatom, group II consisting of fairly large and active motile species, group III which was attaching tightly to substrate or was small non-activemotile diatom, and the group of the largest adhesive strength (group IV). Group IV was com-posed of Cocconeis spp., Gomphorsema sp. and Achnanthes sp. The adhesive strength of epiphyticdiatom would have close connection with its cell size and form, motility, and mucous secretion.
The cell number of epiphytic diatom on various seaweeds were 106-108 per gram dry weight. The dominant and subdominant species were Navicula sp., Cocconeis spp., Gornphonema sp., Nitzschia closterium and Synedra tabulata. The species compositions of epiphytic diatom on the different seaweeds were very similar to each other. However, those on Sargassum horneri which excreted extracellular mucus vigorously was particular, that is, Gomphonema sp, occupied more than ninety percent of total epiphytes.抄録全体を表示PDF形式でダウンロード (328K) -
荒井 克俊1986 年 52 巻 5 号 p. 823-829
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーMature ova of chum salmon Oncorhynchus keta were inseminated with milt from brook trout Salvelinus fontinalis and a portion of the eggs were treated with hydrostatic pressure in order to block the second polar body extrusion and to induce allotriploidy. The embryos from eggs exposed to the hydrostatic pressure shock were more viable than embryos from the usual hybridiza-lion. Karyological studies revealed that the latter inviable hybrids had exactly the same inter-mediate diploid karyatype as that of between two parental species, while the former viable hybrids had the allotriploidy comprising two sets of the maternal haploid complement and one set of the paternal haploid complement. A small number of viable larvae which occurred in the usual hybridization were found to be spontaneous allotriploids in biochemical studies using isozymes as gene markers. These facts proved that allotriploidization can lead to increased survival of developing embryos in some hybridization between salmonid species.抄録全体を表示PDF形式でダウンロード (2327K) -
松浦 基, 吉岡 武也, 新井 健一1986 年 52 巻 5 号 p. 831-837
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーInfluences of actin, pH, MgCl2, and urea on actin-activated Mg-ATPase activity of carp and tilapia myosins, along with the reversibility of those influences, were investigated.
The actin activated Mg-ATPase activity of both myosins was affected by the concentrations of actin, MgCl2, and urea or pH. The changes caused were found to be reversible.
The turbidity of myosin suspension was also affected by pH and the concentration of MgCl2.
The present results, along with some other data, suggested that such changes in actin-activated Mg-ATPase activity of fish myosin depend on different states of the filaments formed, and are species-specific.抄録全体を表示PDF形式でダウンロード (494K) -
尾島 孝男, 市川 寿, 西田 清義1986 年 52 巻 5 号 p. 839-845
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーThe temperature dependence of myosin Mg-ATPase activity and of the heat-dissociation rate of myosin regulatory light chains were studied by using the myosins from striated and smooth adductor muscles of akazara scallop, and from surf-clam foot muscle.
1. Mg-ATPase activity of akazara striated adductor myosin showed a significantly decreased Ca-sensitivity above 20°C, whereas those of akazara smooth adductor and surf-clam foot myosins were to a much less extent affected.
2. The regulatory light chains of akazara striated adductor myosin were readily dissociated by heat-treatment at 30°C, even in the presence of 2mM MgCl2, unlike those of surf-clam foot myosin
3. The Ca2+ concentration for 50% dissociation of regulatory light chains by heat (30°C)- treatment was estimated to be 4μM for akazara striated myosin, 0.8μM for akazara smooth myosin, 0.2μM for surf-clam foot mysoin. The Mg2+ concentration for the 50% dissociation was 240, 20, and 16μM, respectively.抄録全体を表示PDF形式でダウンロード (526K) -
岸村 栄毅, 尾島 孝男, 西田 清義1986 年 52 巻 5 号 p. 847-851
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーA light chain mixture was obtained from the dorsal muscle myosin of Pacific pomfret Brama japonica and Alaska pollack Theragra chalcogramma by subsequent treatments witn 4M urea (+EDTA) and 33% (v/v) acetone. Three light chains designated LC-a, LC-b and LC-c were isolated from each mixture by DEAE-cellulose column chromatography.
LC-b of each myosin was found to bind to desensitized akazara myosin at a maximum molar ratio of 2:1, as did the molluscan regulatory light chain or rabbit DTNB-light chain.
Hybridization of each fish myosin LC-b with desensitized akazara myosin resulted in the decrease of Mg-ATPase activity in the presence and absence of Ca2+, along with loss of Ca2+ sensitivity.
It was concluded from these results that LC-b from each fish species has a similar property to that of rabbit skeletal DTNB-light chain.抄録全体を表示PDF形式でダウンロード (918K) -
鹿山 光, 平田 稔, 久井 哲夫1986 年 52 巻 5 号 p. 853-857
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーThe specific activity of Δ9-desaturase from carp reared at water temperature of 15-18°C was higher than that reared at 28-30°C. The levels of polyunsaturated fatty acids increased in hepato-pancreas microsomes from carp raised in lower temperature. When carp were fed fat-free diet for 62days, specific activity of Δ9-desaturase increased. Supplementation of 5% fatty acids to fat-free diet resulted in the decrease of the specific activity of the enzyme, regardless of the species of fatty acids, including saturated fatty acids. In contrast, suppressing effect of saturated fatty acids to Δ9-desaturase was not observed in vitro. Alloxan injection resulted in decrease and insulin injection resulted in the increase of the specific activity of Δ9-desaturase in carp and was less than in rats. The control mechanism of the enzyme by hormone in carp seems to be reduced.抄録全体を表示PDF形式でダウンロード (403K) -
丹羽 栄二, 甲田 真一郎, 中山 照雄1986 年 52 巻 5 号 p. 859-862
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーIn order to clarify the mechanism of insolubilization of fish proteins induced by freezing, the surface hydrophobicity was compared between actomyosins (AM) before and after freezing. Ten ml of AM solution (0.45M KCl-phosphate buffer, pH 7.5) was frozen in a cellophane-tube at -20°C for two days. After thawing at 4°C, 1ml of 1% (w/v) sodium dodecyl sulfate (SDS) was added, shaken for 1 hr and dialyzed overnight against 20ml of water. The concentration of SDS diffused into the outer liquid was somewhat lowered on freezing for fish AM, while it was not for poultry AM. However, this lowering was depressed on the addition of cryoprotective substance such as sucrose or sodium glutamate.
From the above results, there arose a possibility that the hydrophobic interactions between AM molecules were responsible for the insolubilization of fish proteins induced by freezing.抄録全体を表示PDF形式でダウンロード (351K) -
高橋 計介, 森 勝義, 野村 正1986 年 52 巻 5 号 p. 863-868
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーOccurrence and partial characterizati of lysozymes in the oyster Crassostrea gigas, and the mussel Mytilus edulis, were investigated. Lysozymes were fractionated on a chitin-coated cellulose column. The lysozyme activities were measured using both Micrococcus iysodeikticus and glycol chitin as substrates. In the oyster, high lysozyme activity was observed in the extracts of the digestive diverticula. Lower activities were detectable in the extracts of the gill, mantle, CrystallIne style, and in the hemolymph, though no activities were found in the adductor muscle. Similar results were obtained in the mussel. the oyster lysozyme activity was maximal at pH 5.5 and 50°C, whereas the mussel one was maximal at pH 5.5 and 60°C. The enzyme activities were inhibited mainly by Feg+ and iodine, whereas they were enhanced slightly by Ca2+ and Na+. Finally, a possible relationship between lysozyme and bactericidin was discussed.抄録全体を表示PDF形式でダウンロード (448K) -
鴻巣 章二, 品川 明, 山口 勝己1986 年 52 巻 5 号 p. 869-873
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーA method is described for the HPLC analysis of glycinebetaine, β-alaninebetaine. and γ-butyrobetaine in aquatic animals. The betaines in marine animals were extracted with 80% methanol and converted to p-bromophenacyl esters by treatment with p-bromophenacyl bromide in the presence of 18-crown-6 and KH2PO4. The esters of betaines having UV absorption maximum at 262nm were separated on Shimpack FLC-CN using 10mM KH2PO4 containing 2% acetonitrile, pH 2.10 as a mobile phase, The recoveries of betaines added to the muscle of marine animals were in the range from 93 to 104%.抄録全体を表示PDF形式でダウンロード (332K) -
加藤 登, 橋本 昭彦, 野崎 恒, 丸山 勉, 新井 健一1986 年 52 巻 5 号 p. 875-880
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーSalted meat pastes from Alaska pollack and tilapia were cooked in a water-bath at 35°C to induce the setting. During the setting, the jelly strength of the paste and several properties of myofibrils prepared from the paste were investigated with a lapse of cooking time.
The gelation of Alaska pollack meat paste proceeded more rapidly than that of tilapia meat paste; the maximal jelly strength was obtained at about 2h-setting for Alaska pollack and at 10h-setting for tilapia, respectively. Solubility of myofibrils in 0.5M KCl, viscosity of the soluble protein, and myofibrillar ATPase activity were all decreased as the jelly strength of the source paste was increased; when the jelly strength was reduced to about 40% of the maximum, a bulk (80-90%) of the original biochemical properties was lost for both fish species.
Microscopic observation of the myofibrils revealed that the meat paste was coagulated with the size growing larger as the setting proceeded. In addition, SDS-polyacrylamide gel electro-phoresis showed that myosin heavy chain in the meat paste gradually disappeared as the setting time prolonged.
These results indicated that heat-induced gelation of the salted meat paste was caused by the denaturation of myofibrillar protein in it. It was further suggested that the above changes oc-curred more rapidly for cold-water fishes than tropical-water fishes.抄録全体を表示PDF形式でダウンロード (813K) -
Attaya Kungsuwan, 野口 玉雄, 加納 碩雄, 橋本 周久1986 年 52 巻 5 号 p. 881-884
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーConditions for the assay of tetramine (TM) in the salivary gland of gastropods were studied A dose-death time curve of TM in mice (ddY, males, 18-20g) was prepared using authentic TM chloride. This curve made it possible to examine the extraction conditions of TM from the gas-tropod salivary gland. Based on the results obtained, along with the dose-death time curve of TM chloride, the following assay procedure for tetramine was proposed.
Gastropod salivary gland is homogenized with five volumes of methanol and heated under reflux for 30 min. The homogenate is centrifuged, and the supernatant (or its dilutions) is injected into a group of five mice intraperitoneally. The median value of the death times is converted into mouse units or the TM chloride content in the salivary gland.抄録全体を表示PDF形式でダウンロード (243K) -
藤井 建夫1986 年 52 巻 5 号 p. 885-888
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーThe efficiencies of a Stomacher and a homogenizing blender to prepare bacterial suspensions from seafoods were compared to each other, For 31 of 40 samples, the number of colony forming units obtained through 30-s stomaching was significantly, lower than that obtained through subsequet 1-min homogenizing. In case of dried or semi-dried fish, the release ratio of bacteria by 30-s stomaching was 20-79% of that obtained through subsequent 1-min homogenizing and it was not recovered by prolonged stomaching. The number of colony forming units obtained through 1-min homogenizing did not differ even after subsequent 30-s stomaching.
From the above findings, stomaching was not considered to be enough for preparing bacterial suspensions from seafoods compared with homogenizing.抄録全体を表示PDF形式でダウンロード (291K) -
佐藤 健司, 吉中 禮二, 佐藤 守, 池田 静徳1986 年 52 巻 5 号 p. 889-893
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーThe muscle of carp was fractionated into water-soluble, alkali-soluble (0.1N NaOH), acid-soluble (0.5M acetic acid), hot water-soluble (extraction with water at 120°C for 1h), and residual fractions. The analyses of amino acid and SDS-polyacrylamide gel electrophoresis showed that collagen in the muscle was quantitatively recovered only in the acid-soluble and hot water-soluble fractions and non-collagenous proteins were absent in these fractions. On the basis of the colori-metric determination of protein in these fractions, we devised a simplified method for determinating the soluble and insoluble collagens in the muscle.
The soluble and insoluble collagens in the muscles of the rainbow trout Salmo gairdnerii, Japanese mackerel Scomber japonicus, carp Cyprinus carpio, and eel Anguilla japonica were deter-mined by the proposed method. The total collagen contents in the muscles of rainbow trout, Japanese mackerel, carp, and eel were found to be 0.47, 0.50, 0.60, and 1.99% of the wet muscle, respectively.抄録全体を表示PDF形式でダウンロード (588K) -
上野 隆二, John Liston, 堀口 吉重1986 年 52 巻 5 号 p. 895-900
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーhe present work was undertaken to investigate the intracellular distribution of the enzymes such as cytochrome C oxidase (the mitochondrial. marker), β-glucuronidase, β-N-acetylglucos-aminidase, acid phosphatase and cathepsin D (the lysosomal markers), catalase (the peroxisomal marker) and alkaline ghosphatase (the microsomal marker) in white and red muscles of mackerel according to differential centrifugation. Some properties of both muscle lysosomes were also examined.
In the white muscle, the highest relative specific activities of cytochrome C oxidase, and the four lysosomal enzymes, catalase and alkaline phosphatase were found in the mitochondrial and lysosomal fractions, respectively. In the red muscle, the highest relative specific activities of cytochrome C oxidase, the four lysosomal enzymes, and catalase and alkaline phosphatase were in the mitochondrial, lysosomal and microsomal fractions, respectively.
The buoyant densities of white and red muscle lysosomes were determined to be 1.03g/ml by iso-osmotic self-generating gradients composed of modified colioidal silica (Percoll), but about the same amount of lysosomes having higher buoyant density, ahout 1.04g/ml, was found in red muscle.
The maximum stability of the muscle lysosomes was observed in sucrose over 0.2M and at pH 7. By incubating at 37°C, the fragility of red muscle lysosomes was significantly effected as compared with the white muscle lysosomes. The difference in fragility between red and white muscle lysosomes of mackerel was greater than that of carp.抄録全体を表示PDF形式でダウンロード (416K) -
石尾 真弥, 桑原 将人, 中川 久機1986 年 52 巻 5 号 p. 901-911
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーFe-bound P in the sediment was suspected to be the cause of the frequent red tide in Hakata Bay in summer, because it releases PO43- into the upper water when H2S is generated in the sediment. Thus, the contents of Fe-bound P were measured by applying “H2S-extraction method” to the summer sediments in Hakata Bay and Ariake Bay where red tide scarcely occurs. Their contents were 34±20 ppm in Hakata Bay and 42±21 ppm in Ariake Bay, only 7% and 8% to their total P, respectively. The contents of Al-bound P, Ca-bound P, total P and residual organic P were also measured in these sediments. The contents of Al-bound P were 47±36 ppm in Hakata Bay and 190±211ppm in Ariake Bay, 10% and 35% to their total P, respectively. The contents of residual organic P were 255±109 ppm in Hakata Bay and 140±101 ppm in Ariake Bay, 53% and 25% to their total P, respectively. There was not much difference observed in the con-tents of total P and Ca-bound P between these two bays. The distributions of Fe-and Al-bound P revealed that Fe-bound P does not decrease so quickly as AI-bound P does in the sediments. Then, the solubilities of FePO4-P and AlPO4-P which are the representatives of Fe-bound P and AI-bound P were checked and it was found that the solubility of FePO4-P is smaller than that of AIPO4-P at seawater pH. This suggested that AIPO4-P may convert to FePO4-P in these sediments. This conversion was soon confirmed experimentally. Fe-bound P does not seem statically to be the source of PO43- as the cause of red tide because of their low contents in sediments. However, AIPO4-P from river water converts to Fe-bound P in sea sediments and newly produced Fe-bound P supplies PO43- into the upper water. Therefore, we can say that Fe-bound P is dynamically the causal substance to red tide.抄録全体を表示PDF形式でダウンロード (772K) -
Nazrul MD. Islam, 元広 輝重, 板倉 隆夫1986 年 52 巻 5 号 p. 913-917
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーInhibitory effect of clupeine and salmine sulfates on the growth from the spores of Bacillus sub-tilis and Bacillus licheniformis was investigated. These two protamines were found effective in pre-venting the growth from the spores in both agar and broth media. The level of protamine required to inhibit the growth was found to be more or less dependent on the type of media used and the pH. In general MIC of the protamines ranged from 100 to 150μg/ml in nutrient agar and 10 to 50μg/ml in broth but higher protamine level was required in nutritionally rich Heart Infusion Broth and Penassy Broth. Inhibitory effect of protamine was more profound in the neutral and alkaline pH than in the acidic condition. Filter sterilized and autoclave sterilized protamine had the same inhibitory effect. Spore density in the medium influenced the required level of protamine for preventing the growth from the spores.抄録全体を表示PDF形式でダウンロード (356K) -
Nazrul MD. Islam, 元広 輝重, 板倉 隆夫1986 年 52 巻 5 号 p. 919-922
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーCombined effects of heat treatment and clupeine sulfate on the growth from the spores of Bacillus subtilis and Bacillus licheniformis were studied. The effectiveness of protamine in pre-venting the growth from the spores was considerably influenced by heat treatment at 95°C. In general, increasing the length of heating time at 95°C, decreased the required protamine level in the prevention of growth from the spores or the presence of higher level of protamine decreased the heating time. In nutritionally rich media with higher concentration of organic substances, higher level of protamine and longer heating time was necessary for the prevention of growth. D value for the thermal death of B. subtilis heated at 90°C were 17 and 14.5 min for control and with 200μg pratamine/ml respectively. This meant a 15% decrease in the D value of the spores when heated along with protamine.抄録全体を表示PDF形式でダウンロード (304K) -
坂口 守彦, 河合 章1986 年 52 巻 5 号 p. 923
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (96K) -
鹿山 光, 佐道 哲也, 飯島 憲章1986 年 52 巻 5 号 p. 925
発行日: 1986/05/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (92K)
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