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Volume 52, Issue 8
Displaying 1-26 of 26 articles from this issue
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Hiroshi Kitani1986 Volume 52 Issue 8 Pages 1285-1288
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSLarval development in naupliar stage of Penaeus aztecus was studied and compared with earlier study. Nauplii of penaeid shrimps molt frequently for their growth, and five naupliar substages were reported in the earlier study, but the present study reveals that they pass six naupliar stages to reach the first protozoea. It is also revealed that the naupliar development of this species is very similar to that of the other Penaeus shrimps such as P. duorarum, which is distributed in the Gulf of Mexico, and other species distributed on the Pacific coasts.View full abstractDownload PDF (268K) -
Hideo Sekiguchi1986 Volume 52 Issue 8 Pages 1289-1294
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSBased on zooplankton samples collected in the Kumano-and Enshu-nada seas north of the Kuroshio Current, phyllosoma larvae were examined, with the result that those of eleven species, belonging to five genera Ibacus, Parribacus, Scyllarides and Scyllarus (Scyllaridae) and Panulirus (Palinuridae), are identified. Among these larvae, five species of Scyllarus and two species of Panutirus are described herein. Further, keys to the genus and species of late stages of scyllarid and palinurid phyllosoma larvae in the Japanese waters are represented.View full abstractDownload PDF (416K) -
Masahiro Ueno1986 Volume 52 Issue 8 Pages 1295-1300
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe present paper attempted to estimate the mesh retention of a plankton gauze, NIP #60 (0.33×0.36mm mesh width), widely used for eggs and larvae of fish in Japan. Catches by a gear with NIP #60 were compared with ones by another that retains larvae of Japanese anchovy Engraulis japonicus and gizzard shad Konosirus punctatus of all sizes. The obtained results in-dicated that 29% of japanese anchovy pre-larvae and 41% of gizzard shad pre-larvae were retained. Moreover, A some post-larvae of japanese anchovy less than 5.5mm long evidently passed through the mesh. However, few if any anchovy eggs were extruded through the mesh.
While a theoretical consideration supports the hypothetical “diagonal rule” for slowly towed nets with some synthetic gauzes like NIP #60, the results do not agree with the “rule”. It is con-cluded that wide variations in mesh sizes on NIP #60 caused this conflict. The maximum cross-sectional diameter of an organism must be greater than the maximum mesh diagonal on NIP #60 if it is to be retained.View full abstractDownload PDF (435K) -
Masahiro Ueno1986 Volume 52 Issue 8 Pages 1301-1306
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe fiducial limits of the catch provided by a single plankton sample may be estimated by the repeated sample. In order to estimate the limits (TFL), traditionally, the data were transformed to logarithms. The log-transform was expected to have the effect of removing the correlation between variance and mean, thus stabilizing the variance to more-or-less constant value, so that valid analysis of variance could be applied to the data.
Although the data are transformed to logarithms, the catches of anchovy eggs and pre-larvae taken with ten repeated hauls show that the variances apparently depend upon the means, es-pecially provided the sample size is small (100 or less), so that the analysis of variance may be misleading.
New fiducial limits (NFL) are made with a pair of catches, C1 and C2. Suppose that C1 and C2 are sampled from a hypothetical population following a negative binomial distribution with a constant 1/k. Then the NFL |D| can be expressed by means of the formula,
|D|≤tp√(1/m-1/k)/2 where m=(C1+ C2)/2, D=(C1-C2)/(C1+C2) and tp is the value for p% in the standard normal distribution. Compairing NFL with TFL by m-D diagram for anchovy data, it was proved that NFL fitted better than TFL when the sample sizes are widely distributed. Using m-D diagram, moreover, only two repeated samples on each station give much information on the distribution of catches there.View full abstractDownload PDF (404K) -
Taeko Kitahara, Hitomi Hirose, Takashi Hibiya1986 Volume 52 Issue 8 Pages 1307-1312
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe reproductive organs of a monogenetic trematode, Diplozoon nipponicum, were histologically observed during non-spawning period, from November 1982 to April 1983. The parasites were taken from the gill of common crucian carp Carassius auratus subsp. and C. auratus langsdorft caught in the Hirai and Aki River of the Tama River water system. The parasites were observed in all months examined.
Even during non-spawning period, oocytes and spermatozoa were in their gonads, whereas yolk cells in the vitelline glands distinctly decreased both in size and number with the decline of water temperature. These results indicate that, in gonadal maturation, the development of vitel-line glands is most clearly affected by water temperature among the reproductive organs.View full abstractDownload PDF (2200K) -
Osamu Imada, Yuichi Saito1986 Volume 52 Issue 8 Pages 1313-1321
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe composition of artificial seawater and the carbon dioxide source were investigated by the culture test of layer thalli. Culture test results were as follows.
1. About the screening of more suitable masking agents for the trace metal solution (Pl sol.) in place of ethylenediaminetetraacetic acid (EDTA), four chelaters diethylenetriaminepenta-acetic acid (DTPA), 1, 2-cyclohexanediaminetetraacetic acid (CDTA), N-hydroxyethylenedi-amine-N-N'-N″-triacetic acid (HEEDTA) and nitrilotriacetic acid (NTA) were examined. The two former salts, DTPA and CDTA had approximately the same and the two latter salts, HEEDTA and NTA had somewhat less influence than EDTA on the growth rate of laver thalli.
2. The adding volume change and the changing of the ratio of chelater and heavy metal salts of Pl sol. were investigated based on modified ASP 6 culture medium after Suto. Adding of Pl sol. was examined by six steps of the volume ratio (0, ×0.5, ×1, ×2, ×5, ×10). Two levels (×1, ×2) were selected as suitable for layer culture. The ratio of chelater and heavy metal salts were examined by six steps of the ratio (0/1, 0.3/1, 0.75/1, 1.5/1, 5/1, 10/1), and three ratio levels (0.75/1, 1.5/1, 5/1) were selected as suitable about layer culture. The adding volume change and the changing of the ratio of chelater and heavy metal salts of Pl sol, were not so serious for the thalli culture but obvious decrease of the germination of conchospore were recognized.
3. Alt appearances indicate that there are no negative effects, using NaCl and MgSO4 salts of the industrial grade in place of the agent grade for artificial seawater as shown by the culture test of layer.
4. The culture tests of layer thalli using the combustion vent gas from the boiler burning A class heavy oil in place of pure gasified liquid CO2 were investigated. The combustion vent gas was more suitable than pure CO2 for the culture of layer, having an additional sterilizing function caused by the sulfer dioxide present in it.View full abstractDownload PDF (677K) -
Osamu Imada, Tomoatsu Usuku, Shin Ando, Yuichi Saito1986 Volume 52 Issue 8 Pages 1323-1338
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSApilot plant for economical study of artificial culture of layer was designed and constructed based on series of 10m3 main culture, 2m3 test culture, 400l pre-culture, germinating vessels and artificial seawater make-up, CO2 dissolving vessels, etc. Utilities supply systems, e.g. aeration, seawater circulation and cooling, the instrumentation, e.g. temperature control, continuous record-ing of temperature, pH and light intensity, were also provided.
Gulture vessels were constructed by using carbon steel and lining the inner side with a white hard PVC sheet. They have cooling pipes, light sources, an air bubbler and some detectors for the instrumentation as accessories. Cooling pipes were made from AISI 316L, and accessories were made from PVC, pyrex glass or polymethacryl resin. These materials were determined byprevious laver culture tests and confirmed non-toxic performance.
Culture vessels were designed as cylindrical vessels with air sparger and draft tube, based on the test results of liquid mixing and thalli circulating performances.
The on-off set controller only D action and solenoid valve were selected for temperature control of culture by regulating coolant and realized suitable temperature control economically.
Screen filter with rotary slit moving by water flow was designed for separation of layer thalli from seawater effectively.
The experimental system of a pilot plant worked satisfactorily for about 5 years in artificial culture tests of layer thalli on a larger scale and introduced cultural data for practical problems.View full abstractDownload PDF (2302K) -
Toyomi Takahashi, Tatsuaki Maeda, Toshikuni Nakatani, Nobuyuki Yanagaw ...1986 Volume 52 Issue 8 Pages 1339-1349
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to elucidate the conditions of food environments for righteye flounders Limanda herzensteini and L. yokohamae in Mutsu Bay, Aomori Prefecture, the distributions of macrobenthos were investigated by analyzing grab samples obtained at approximately 300 stations chiefly from March 1980 to February 1983. Faunal composition in the bay was correlated with the mud contents in the bottom sediments. In the extensive mud bottom in offshore area polychaete annelids predominated, representing 82.8% of the total number of macrofauna. Most of the dominant species, Scalibregma inflatum, Paraprionospio pinnata, and Maldane sarsi, were chiefly abundant in East Bay. So macrofaunal biomass and density in West Bay were consistently lower than those in East Bay. The densities of S. inflatum and Ampharete sp. increased remarkably in spring and decreased in autumn and winter, while no seasonal periodicity was found in fluctuations in den-cities of Lumbrineridae and M. sarsi. There is a general tendency that the macrofaunal biomass in offshore area attains peak in summer and lowest in winter. But such a periodicity was not found in East Bay since the autumn of 1980 because of remarkable increase in density of M. sarsi. Results of cluster analysis showed that the community structure of macrofauna in offshore area varied largely with fluctuations in abundance of dominant polychaetes by seasons and years.View full abstractDownload PDF (826K) -
Tadashi Sakai, Ken-ichi Yamamoto, Makoto Endo, Akira Kuroki, Kazumi Ku ...1986 Volume 52 Issue 8 Pages 1351-1354
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe activities of carbonic anhydrase (EC 4. 2. 1. 1) in the gills of yellowtail Seriola quinqueradiata and red sea bream Pagrus major exposed to Chattonella marina red tide were measured.
When yellowtail and red sea bream were exposed to the seawater containing C. marina of 2, 900 and 6, 500 cells/ml, respectively, the gill enzyme activities were rapidly decreased. After about 40 min, these were not detectable.
To simulate this phenomenon by inhibiting gill carbonic anhydrase in vivo, yellowtail were exposed to the seawater containing 0.2mM acetazolamide, a potent and specific inhibitor of the enzyme, and 5 ppm Triton X-100 which was used to remove the mucus from the gills. The fish swam violently in proportion to the duration of exposure and were fatal in about 60 min. After 60 min, the oxygen partial pressure in the arterial blood was reduced to 25-40% of the pre-exposure value.
Judging from these results, the reduction of carbonic anhydrase activity in the gills may be one of the principal causes of mortality by the exposure of fish to C. marina.View full abstractDownload PDF (299K) -
Makoto Terazaki, Daiji Kitagawa, Yoh Yamashita1986 Volume 52 Issue 8 Pages 1355-1358
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSVertical distributions of Euphausia pacifica HANSEN were investigated in the vicinity of Otsuchi, the Sanriku coast of northeastern Japan, in the spring of 1981 and 1982. Many females with attached spermatophore and male with empty ejaculatory ducts were distributed below 100m along Todogasaki Section and Ozaki Section despite no remarkable thermocline. Females with sper-matophore were distributed below 15m depth throughout the whole day at Stn. A located near the mouth of Otsuchi Bay. Females with spermatophore occupied 45.0% of the total number of females collected from Stn. A. Minimum body length of males with at least one empty ejacala-tory duct and females with spermatophore was 14mm. Main breeding location of E. pacifica is the shallow waters near the coast and their copulation takes place in mid-water.View full abstractDownload PDF (280K) -
Haruyasu Kimura, Yukihiro Munekage, Hisayosi Watanabe1986 Volume 52 Issue 8 Pages 1359-1363
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSValues of diffusion coefficient and load of dissolved or suspended matter in the same sea areaand period must take same values regardless of models. When over two models were applied tothe same sea area, the adequacy of applied model can be examined by comparing the diffusioncoefficient and load of dissolved matter.
Therefore, two models-one layer one box model and one layer four box model-were applied to Komame Bay in Kochi prefecture. The accuracy of box models which were applied to Komame Bay was investigated from diffusion coefficient in the section at the mouth of the bay, load of dis-solved oxygen and inorganic nitrogen into the bay.View full abstractDownload PDF (311K) -
Toshio Okazaki1986 Volume 52 Issue 8 Pages 1365-1376
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSGenetic variation and population structure among masu salmon river populations in Japan were examined. Enzymatic proteins in tissue samples of fish among twenty populations collected from eighteen rivers were assayed for electrophoretically detectable variants. 34 loci were tenta-tively identified, 17 (50.0%) of which were polymorphic. Significant differences in the frequencies of alleles were observed even among the proximal river populations. The magnitude of genetic divergence among masu salmon river populations is relatively large among Pacific salmon, On-corhynchus spp. While stability of allelic frequencies was observed between annual samples from same river systems. According to the resulting dendrogram, the examined populations appeared to be roughly divided into two geographic groups at the Shiretoko Peninsula of Hokkaido; (1) the rivers entering the Strait of Nemuro and the Pacific Ocean, (2) the rivers entering the Okhotsk Sea and the Japan Sea. The obtained level of the average heterozygosity of masu salmon was 5.2%.View full abstractDownload PDF (2109K) -
Kumiko Shindo, Takahide Tsuchiya, Juichiro J. Matsumoto1986 Volume 52 Issue 8 Pages 1377-1399
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA histological study was made on dark and white muscles of four freshwater fishes and foursaltwater fishes.
In myofibrils, difference of cross sectional patterns was found between dark and white muscles of 8 fish species. Mitochondria in dazk muscles were observed not only around myofibrils but also in myofibrils, whereas they were located around myofibrils in white muscles. All the species studied here, with the exception of white muscle in blackthroat seaperch, were found to have I-bands which are narrower than in mammalian muscles. On the contrary, the I-bands of white muscles in blackthroat seaperch were almost similar to the skeletal muscles of rabbit.
In dark muscle of Japanese char, rainbow trout, horse mackerel and mackerel, lipid droplets were found in the interspaces of myofibrils and there were many mitochondria surrounding the droplets.
The dazk muscles of Japanese char, rainbow trout and blackthroat seaperch appeared struc-totally very similar to one another. These dark muscles were much smaller in size and less darker in color as compared with the dark muscles of other fishes studied here.View full abstractDownload PDF (14336K) -
Michio Kawada, Takeshi Shibata1986 Volume 52 Issue 8 Pages 1401-1407
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to obtain further information on the regulation of glycogen metabolism in fish skeletal muscle, the conversion of carp phosphorylase b into a was investigated and compared with that of rabbit phosphorylase by using both carp and rabbit phosphorylase kinases.
Although both the carp and rabbit phosphorylases are phosphorylated and converted to the active form by eitber carp or rabbit phosphorylase kinase, the amount of phosphorylation, the extent of activation and the occurrence of polymerization differed between carp and rabbit phos-phorylase. The carp phosphorylase incorporated one mol of phosphate per 200000g of the enzyme, activated only 50% of the total phosphorylase activity and was not accompanied by a dimerization of the enzyme during the conversion reaction from b to a.
From the above results, it is suggested that the carp active phosphorylase is a dimer and a hybrid type containing one phosphorylated and an other non-phosphorylated subunit.View full abstractDownload PDF (2383K) -
Michio Kawada, Takeshi Shibata1986 Volume 52 Issue 8 Pages 1409-1416
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to obtain further information on the regulation of glycogen metabolism in fish skeletal muscle, the activation and phosphorylation of carp phosphorylase kinase were investigated by using both carp and rabbit cyclic AMP-dependent protein kinase and compared with those of rabbit phosphorylase kinase.
The carp cyclic AMP-dependent protein kinase activated and phosphorylated in a similar fashion as rabbit or carp phosphoryiase kinase, just as the mammalian counterpart does. In the cyclic AMP-dependent kinasc-catalyzed reaction, the carp phosphorylase kinase shows a major difference in the patterns of activation and phosphorylation in that unlike those of rabbit phos-phorylase kinase, no correlation between them could be demonstrated and the enzyme activationwere not significantly affected by the incorporation of phosphate. The 32P incorporation into α andβsubunit of the carp enzyme are 45% and 70%, respectively, as compared with that of each subunit of the rabbit. The pH 6.8 to 8.2 enzyme activity ratio is changed only 1.5-fold from 0.27 to 0.41 following phosphorylation of the carp enzyme. And also, the carp enzyme activity is unaffected by phosphorylase kinase phosphatase. It is characterized that the purified carp phosphorylase kinase exists in a non-activated state having a higher activity ratio of pH 6.8 to 8.2.
From these results, it is suggested that phosphorylation-dephosphorylation mechanism may play a new role in the regulation of carp phosphorylase kinase and there may be the modified function of a hormonal control of glycogen metabolism in carp muscle.View full abstractDownload PDF (1367K) -
Shin-ichi Teshima, Akio Kanazawa1986 Volume 52 Issue 8 Pages 1417-1422
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFeeding experiments were conducted using 5 test diets to examine the nutritive value of several sterols for the juvenile prawn Penaeus japonicus. The prawn showed a markedly lower weight gain, feed conversion efficiency (FCE), and protein efficiency ratio (PER) on a sterol-deficient diet, than diets with 0.5% 1evels of cholesterol, β-sitosterol, β-sitosterol-cholesterol (9:1, w/w), or 24-methylcholesta-5, 22-dienol as sterol sources. Cholesterol was most effective among the sterols examined in improving the weight gain, FCE, and PER Although both 24-methylcholesta-5, 22-dienol and β-sitosterol were effective in improving growth, the former had a higher efficacy than the latter. The addition of small amounts of cholesterol to a β-sitosterol-diet improved the FCE and PER somewhat, but, not the gain in weight. The retention rates (%) of dietary proteins and lipids to the body were markedly lower in the prawn receiving the sterol-deficient diets than in those receiving the diets containing sterols. The retention rate of dietary total sterols to the body varied with the kind of sterols in diets, the highest retention rate being observed on a cholesterol-diet. Dietary 24-methylcholesta-5, 22-dienol and β-sitosterol were less retained in the body than dietary cholesterol, although the two former sterols were likely to be converted to cholesterol in the body.View full abstractDownload PDF (418K) -
Mutsuyosi Tsuchimoto, Teruhiro Utsugi, Tosio Misima, Shunichi Kitajima ...1986 Volume 52 Issue 8 Pages 1423-1430
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTo clarify the actual situation of freshness of fish at landing plates of Thailand and its change during transportation and marketing, we measured K values of trawl-caught fish at fishing ports and retail markets in four major fishing ports of Thailand, Phuket, Paknam, Songkhla, and Bangkok.
The mean K values just after landing differed by fishing ports; we obtained 39.4% at Songkhla, 34.3% at Paknam, and 36.2% at Bangkok, contrasting with a significantly low value of 26.0% at Phuket. The last-named is located on the Andaman Sea side, while others are on the Gulf of Thailand. Levels of K values also fluctuated from boat to boat. Fish landed by fishing boats operating in the Gulf of Thailand which stayed more days at sea for a cruise than fishing boats operating in the Andaman Sea, showed higher K values. The mean K values of fish landed by fishing boat were found to be in a positive correlation with days at sea of the boat, increasing at a rate of 2.2% per day at sea.
The mean K values observed at retail markets were 52.3% in Paknam facing the Gulf of Thailand which was significantly higher than 29.0% observed in Phuket located on the Andaman Sea side. Increase in K values during transportation and marketing, from landing to consumers, was revealed to be at a rate of 0.9 to 1.5% per hour. Taking into consideration the high environ-mental air temperature of the tropical country, the rate mentioned above was rather very low. Early progress of bacterial contamination of fish caught in Thailand was suggested by the rise in pH when K values were comparatively low.View full abstractDownload PDF (599K) -
Mutsuyosi Tsuchimoto, Tosio Misima, Teruhiro Utsugi, Shunichi Kitajima ...1986 Volume 52 Issue 8 Pages 1431-1441
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTo study temporal changes in K values (a freshness index) of fish muscle: sample fishes com-prising of 82 species were collected from fishing grounds of the tropical, subtropical and temperate waters ranging from 28°C to 9°C in bottom temperatures. K values of fish kept in water of 32°C since killing, increased linearly with the lapse of time. The slope of the regression line for a fish was defined as the increasing rate of K values at 32°C, and designated as ΔK32, which can be used as an indicator to express the species specific speed of lowering freshness of the fish. This indicator enables us to formulate speeds of lowering in freshness for fishes of different species and from various waters. With the bottom temperature (t) of fishing grounds, ΔK32 related reversely as ΔK32=-0.4708t+19.240. That ΔK32 of the fishes inhabiting the tropical waters were generally lower than those of the fishes in the subtropical and temperate waters may explain the fact that freshness of fishes at catching, landing and in retail markets in Thailand was much better than one might expect. Adaptation to the warmer waters was suggested by higher ΔK32 of fishes living in cooler waters than those of the same fishes living in warmer waters. Putting increasing rate of K values for the fish kept at various temperatures (t) as ΔKt, this related with t as ln ΔKt=0.10413t+b.View full abstractDownload PDF (763K) -
Toshiaki Itami, Yukinori Takahashi, Itsuro Kawahara1986 Volume 52 Issue 8 Pages 1443-1447
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe bacteriolytic activity was examined on the homogenates of some organs of Ayu Pleco-glosses altivelis: the mucus on the body surface, kidney, intestine, mucus on gill surface and serum. The enzymatic properties of the bacteriolytic activity of the skin mucus and kidney were also in-vestigated. The activity was determined by the turbidimetric method using acetone-ether-dried sells of Micrococcus lysodeikticus as the substrate.
The skin mucus showed the highest bacteriolytic activity, followed in order by the kidney, intestine and gill mucus, but the serum didn't lyse the cells. The maximal activity of the bacterio-lysis by the skin mucus appeared in 0.0075M phosphate buffer of pH 6.5 at 35°C. And the kidney showed the maximal activity in 0.01M phosphate buffer of pH 7.0 at 35°C. The bacterio-lytic activity of both the skin mucus and the kidney heated at 100°C for 10 min in the acidic buffer (pH 3.5) were relatively stable but those in neutral (pH 7.0) or the alkaline (pH 9.0) buffer were liable to be inactivated, as shown by the lysozyme from the hen egg-white.View full abstractDownload PDF (1141K) -
Kenji Takii, Sadao Shimeno, Masahiko Takeda, Shuji Kamekawa1986 Volume 52 Issue 8 Pages 1449-1454
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSPepsin-like, trypsin-like and amylase activities in the gastrointestinal tissue and their digesta were assayed at 3 and 6h interval after feeding a diet flavoured with alanine, glycine, proline, his-tidine and uridine-5'-monophosphate on the initial day, day 13 and final feeding day of the experi-ment.
Eel reared on the flavoured diet for 25days showed remarkably high growth, feed efficiency and energy retention compared with the control group. Although a similar change was recognized in gastric digesta weight of both groups, the flavoured group had significantly higher pepsin-like activities in gastric digesta than the control group, A significant positive correlation between the intestinal digesta weight and trypsin-like activities and high digestibility of dietary protein and carbohydrate were observed in the flavoured dietary group. Whereas higher trypsin-like and amylase activities and digesta weight in intestinal tract were determined in the control group, no rela-tionship between intestinal digesta weight and their trypsin-like activity was recognized. These findings suggest that the feeding stimulants in the diet, stimulates the feeding activity of the fish and promotes the digestion and absorption of the diet.View full abstractDownload PDF (436K) -
Chiaki Imada, Saburo Hara, Masachika Maeda, Usio Simidu1986 Volume 52 Issue 8 Pages 1455-1459
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe amino acid sequences of two peptide protease inhibitors (Marinostatins C-1 and C-2) were determined by Edman-degradation. The amino acid sequence of C-1 and C-2 were Phe-Ala-Thr-Met-Arg-Tyr-Pro-Ser-Asp-Asp-Ser-Glu and Gln-Pro-Phe-Ala-Thr-Met-Arg-Tyr-Pro-Ser-Asp-Asp-Ser-Glu, respectively. C-1 and C-2 were identical except for differences in their N-terminal regions, suggesting, that C-1 might be derived from C-2 with deletion of two N-terminal amino acids by proteolytic removal of this region.
From the amino acid sequence analysis, the molecular weight of C-1 was calculated to be 1, 417.5 and that of C-2 to be 1, 643.8. Field mass spectral analysis of C-1 and C-2 gave parent peaks corresponding to the molecular weights of 1, 381.5 and 1, 589.5, respectively. As inhibitory activity is lost in an alkaline pH range (pH>10), it is suggested that 2 or 3 ester linkages might exist in the peptide molecules, which play a part in the inhibitory activity of Marinostatins.View full abstractDownload PDF (382K) -
Yasuo Yone, Abul Hossain, Masayuki Furuichi, Fumio Kato1986 Volume 52 Issue 8 Pages 1461-1464
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe condition factor, % of organ weight, proximate compositions of liver and muscle, hemato-logical characteristics, chemical components and enzyme activities in blood plasma of red sea bream fed on fermented (F) or fermented-resteamed (FS) scarp meal diet for 8 weeks were com-pared with those of nonfermented scrap meal (NF) or white fish meal (W) diet group.
The condition factor, % of liver, intestine, and heart weights, lipid and glycogen contents in liver of NF group were lower and moisture, protein, and ash contents in liver were higher com-pared to W group. In blood characteristics of NF group, RBC was lower, but MCV and MCH were significantly higher than those of W group. Besides this, the total protein and cholesterol in blood plasma of NF group were lower, but total bilirubin, GOT, GPT, and TTT were higher than those of W group. These findings suggest that NF group had a macrocytotic hyperchromemia and a slight liver malfunction. On the other hand, no significant differences were found between W group and F or FS group in all of the investigated parameters, except % of heart weight and MCV between F group and W group.
View full abstractDownload PDF (240K) -
Yasuo Yone, Masayuki Furuichi, Kazunari Urano1986 Volume 52 Issue 8 Pages 1465-1468
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn order to investigate Che nutritional value of seaweed as a dietary supplement, red sea bream Chrysophrys major were fed on the control diet without seaweed and on the diets supplemented with Wakame or Ascophyllum at a level of 5% or 10% to the control diet for 10 weeks. The growth and feed efficiency were best in the 5% Wakame diet group, followed by the 5% Ascophyl-lum, the 10% Wakame, the control, and the 10% Ascophyllum diet group. Besides this, the muscle lipid content was slightly higher in the 5% Wakame, the 5% Ascophyllum and the 10% Wakame diet groups, compared with the control and the 10% Ascophyllum diet groups.View full abstractDownload PDF (230K) -
Hideki Kishimura, Takao Ojima, Kiyoyoshi Nishita1986 Volume 52 Issue 8 Pages 1469-1472
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSCalcium dependence of the Mg-ATPase activity and calcium binding of three myosins was investigated: akazara striated adductor myosin and its hybrid myosins with 20 kd-light chains of chicken gizzard myosin and with DTNB-light chains of rabbit skeletal myosin.
All the three myosins bound two moles of Ca2+ per mole of myosin. One mole of Ca-binding was inhibited by Mg2+ in akazara myosin and the hybrid myosin with the 20 kd-light chains, both myosins whose Mg-ATPase activity showed Ca-sensitivity. On the other hand, two moles of Ca-bindings were inhibited by Mg2+ in the hybrid myosin with DTNB-light chains, the myosin whose Mg-ATPase activity showed no Ca-sensitivity. Desensitized akazara myosin did not bind Ca2+.View full abstractDownload PDF (305K) -
Etsuo Watanabe, Masanobu Fukuda, Tetsuhito Hayashi, Kenzo Toyama1986 Volume 52 Issue 8 Pages 1473
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (75K) -
Kenji Hayashi1986 Volume 52 Issue 8 Pages 1475
Published: August 25, 1986
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (90K)
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