The composition of artificial seawater and the carbon dioxide source were investigated by the culture test of layer thalli. Culture test results were as follows.
1. About the screening of more suitable masking agents for the trace metal solution (Pl sol.) in place of ethylenediaminetetraacetic acid (EDTA), four chelaters diethylenetriaminepenta-acetic acid (DTPA), 1, 2-cyclohexanediaminetetraacetic acid (CDTA),
N-hydroxyethylenedi-amine-
N-N'-N″-triacetic acid (HEEDTA) and nitrilotriacetic acid (NTA) were examined. The two former salts, DTPA and CDTA had approximately the same and the two latter salts, HEEDTA and NTA had somewhat less influence than EDTA on the growth rate of laver thalli.
2. The adding volume change and the changing of the ratio of chelater and heavy metal salts of Pl sol. were investigated based on modified ASP 6 culture medium after Suto. Adding of Pl sol. was examined by six steps of the volume ratio (0, ×0.5, ×1, ×2, ×5, ×10). Two levels (×1, ×2) were selected as suitable for layer culture. The ratio of chelater and heavy metal salts were examined by six steps of the ratio (0/1, 0.3/1, 0.75/1, 1.5/1, 5/1, 10/1), and three ratio levels (0.75/1, 1.5/1, 5/1) were selected as suitable about layer culture. The adding volume change and the changing of the ratio of chelater and heavy metal salts of Pl sol, were not so serious for the thalli culture but obvious decrease of the germination of conchospore were recognized.
3. Alt appearances indicate that there are no negative effects, using NaCl and MgSO
4 salts of the industrial grade in place of the agent grade for artificial seawater as shown by the culture test of layer.
4. The culture tests of layer thalli using the combustion vent gas from the boiler burning A class heavy oil in place of pure gasified liquid CO
2 were investigated. The combustion vent gas was more suitable than pure CO
2 for the culture of layer, having an additional sterilizing function caused by the sulfer dioxide present in it.
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