巻号一覧
53 巻, 1 号
選択された号の論文の27件中1～27を表示しています
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河口 真一郎, 佐野 典達, 水洗 能治1987 年 53 巻 1 号 p. 1-7
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn the former paper, the author described the results of simulation and fundamental experiments by using Frequency Diversity Method which is a new data processing technique for Acoustic Fish Assessment System.
In this paper, we describe the Statistical Characteristics of Volume Scattering Strength at the Frequency Domain by using the above equipment.
The main results obtained are summarized as follows-
The Power Spectrum of SV value is concentrated upon low Frequency Band.
The distribution of the abundance of fish stocks is divided into two parts by 33°02.0'N line in the square-sea area from 33°01.7'N, 33°02.6'N, 131°55.7'E, 131°47.8'E.
SV value which is measured by the sea bottom tracking layer is more conductive to SV value which is measured by the whole layer than the one which is measured by the upper layer.
Time Series Analysis is effective against the analysis of the abundance of fish stocks.抄録全体を表示PDF形式でダウンロード (388K) -
川津 浩嗣, 中西 洋一, 岳田 秀人1987 年 53 巻 1 号 p. 9-14
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーSpectrophotometric procedures for methemoglobin determination in eel blood were investi-gated. Two different procedures using wavelengths at 630nm and 820nm were applied to the blood samples of eel. In normal conditions, the methemoglobin level of the eel blood was esti-mated to be less than 1%by the method of Horecker and Brackett, whereas the method of Dubowski revealed much higher values because of a spectral interference between methemoglobin and cyanmethemoglobin derived. The methemoglobin level of blood sample remained unchanged at 4°C for several hours. Methemoglobin level slightly increased after 24-h exposure to nitrite at 20 ppm. At more than 30 ppm, the level remarkably increased with wide individual variations. In the recovery test, methemoglobin level returned to normal values within 24h.抄録全体を表示PDF形式でダウンロード (388K) -
藤野 和男, 奥村 誠一, 稲吉 宏之1987 年 53 巻 1 号 p. 15-21
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーSuccessful induction of two kinds of triploid abalone animals (abbreviated as 3n.1pb and 3n.2pb respectively), by inhibiting release of first or second polar body, was reported recently.
The present work examines associations between thermostability variations of enzymes at multiple loci and temperature tolerance in terms of survival of animals against high and/or low temperature stresses within each group of diploid and triploid animals, induced artificially, and compared such temperature tolerances between normal diploid (2n) and two kinds of triploid sib animals of the Pacific abalone Haliotis discus hannai. Significantly higher survivals of animals homozygous of thermo-resistant allele at phosphoglucomutase and malate dehydrogenase thermo-stability loci against high water temperature stress as well as of those homozygous of thermo-sensitive allele at malate dehydrogenase thermostability locus against low water temperature stress were observed. Comparison of high temperature tolerances between different ploidy sib animals indicated the greatest survival in the animals of 3n.1pb group, followed by 2n and the least survival in 3n.2pb group. In addition, a combined effect of chromosome-set manipulation and selected genotype of thermostability variations was seen, suggesting a possible immediate application of the results of such observations towards greater productivity in abalone aquaculture through an appropriate selective breeding programme.抄録全体を表示PDF形式でダウンロード (541K) -
浜野 龍夫, 松浦 修平1987 年 53 巻 1 号 p. 23-39
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe Japanese mantis shrimp Oratosquilla oratoria spawned oval eggs, measuring 0.47×0.44mm soon after spawning. Two regression formulae were suggested for the relationship between mean water temperature (X, °C) and the duration of incubation (Y, day), when the animal nursed the disc-shaped egg mass using the thoracopods: Y=58.39-1.85X, Y=111.40/(X-15.26). The hatched larvae developed and metamorphosed to the first juveniles through 11 larval stages over a period of one to two months at 21-28°C. Larval stages and the first juvenile were de-scribed and illustrated in detail. Propelagic larvae in the first and second stages contained yolk and did not feed, but the other nine, pelagic, stages had functional mouth parts and ate a range of sizes of living Artemia cultured in a special medium. Positive phototaxis was observed during the period of the third to the final stages though it diminished as development progressed; larvae in the tenth and eleventh stages often occurred close to the substrate. The first juvenile behaved similarly to the adult. i.e. it lived in an artificial burrow.抄録全体を表示PDF形式でダウンロード (1171K) -
佐藤 要, 武田 誠一, 宮崎 芳夫1987 年 53 巻 1 号 p. 41-46
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe relations among principal items of 8 types of fishing boats were analyzed using Canonical Correlation Analysis, and the features were extracted on the basis of analyzing results, and were compared thoroughly with the actual principal items.
Here, from our studies, three ratios of (fish hold)/(length between perpendiculars×molded breadth×molded depth), (main engine horsepower)/(full load displacement), and (sea speed)/(length between perpendiculars)1/2 are noteworthy as the best expressions of the features of fishing boats.
We adopted these ratios and scalar quantities lead from the basic principal items as the de-pendent variables and the independent ones, respectively, and conducted the analysis.
Consequently, the features of fishing boats of 8 types were obtained. The Canonical Cor-relation Analysis was available here.
Our final purposes are, using our results, grouping for general types of fishing boats, and contributions to the safety of fishing boats and to the elevation of fishing performance.抄録全体を表示PDF形式でダウンロード (530K) -
田中 真, 吉水 守, 木村 喬久1987 年 53 巻 1 号 p. 47-55
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe ultrastructures of RTG-2 cells and hepatocytes of chum salmon (Oncorhynchus keta) infected with Oncorhynchus mason virus (OMV) were observed by thin sectioning and negative staining. RTG-2 cells were fixed 3 and 7 days after OMV infection (m.o.i.=0.01) and the tissue samples from the liver of moribund 80-day-old, 3-month-old and 4-month-old chum salmon infected with OMV by the immersion method (100 TCID50/ml, 60 min) were prepared for electron microscopy. The infected RTG-2 cells and hepatocytes were prefixed with paraformaldehyde-glutalaldehyde and post fixed with 2% osmic acid.
Electron micrographs of both RTG-2 cells and hepatocytes infected with OMV revealed the following activities: nuclei showed the margination and the subsequent aggregation of chromatine; syncytia was found in both RTG-2 cells and hepatocytes; the nuclear membranes became smooth and the virus matured in the nucleus of those cells; the matured virus particles were observed in the vesicle and around the cell membrane; and enveloped virus particles were seen in the focus of the hepatocyte. The size of enveloped virus particles was approximately 200×240nm and the nucleocapsid was 115nm in diameter.抄録全体を表示PDF形式でダウンロード (5258K) -
小栗 幹郎1987 年 53 巻 1 号 p. 57-58
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIt has been described in several books that the fishes of the genus Diodon are aglomerular. To ascertain this, I obtained three specimens of the porcupine fish D. holacanthus and did detailed histological examinations of the kidney tissue using H-E or Bowie's stain. Consequently, I con-firmed the presence of the glomeruli and the juxtaglomerular cells in the kidney tissue. Therefore, the description of Diodon spp. as aglomerular fishes should be corrected.抄録全体を表示PDF形式でダウンロード (982K) -
須田 有輔, 清水 誠, 能勢 幸雄1987 年 53 巻 1 号 p. 59-61
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーMorphological differences between the cultivated and the wild Japanese jack mackerel, Trachurus japonicus are examined as a part of obtaining knowledge on the degree of the intra-specific variation of T. japonicus and in order to find out what kind of morphological variations appear under the cultivated condition.
Comparisons were made for the 31 cultivated and 43 wild (from southwestern Shikoku) fish and the 31 cultivated and 1181 wild (various parts of Japan) fish. 16 morphometric characters were examined.
In the cultivated T. japonicus, the body depth becomes large and the pectoral fin shortens.
These are remarkable external morphological changes seen in both comparisons.
These morphological changes may occur in the spatially limited environment like fish cultiva-tion, because the fish activity is restricted in comparison with that in the natural environment.抄録全体を表示PDF形式でダウンロード (193K) -
良永 知義, 小川 和夫, 若林 久嗣1987 年 53 巻 1 号 p. 63-65
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーNeomysis intermedia (Mysidacea) and Thermocyclops sp. (Copepoda) were examined as possi-ble intermediate hosts for Hysterothylacium aduncum in a freshwater lake, Hokkaido, and only the former was found infected (infection rate 0.49%). The nematode is described and identified as third-stage larva of H. aduncum. The life cycle of H. aduncum in the lake is discussed.抄録全体を表示PDF形式でダウンロード (199K) -
小玉 修嗣, 今野 久仁彦, 新井 健一1987 年 53 巻 1 号 p. 67-76
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーSubfragment one (S1) from squid mantle myosin was subjected to limited proteolysis and analyzed for peptide composition by SDS-polyacrylamide gel electrophoresis. Tryptic digestion of S1 in the absence of actin revealed that l00 kilo daltons (Kd) heavy chain gave rise to three peptides whose molecular weights were 47, 27, and 23 Kd. However, the digestion of S1 in the presence of actin resulted in only two peptides of 70 and 27 Kd. The size and composition of these peptides were similar to those from rabbit skeletal S1. Chymotryptic digestion of S1 re-moved regulatory light chain but not essential light chain, decreased the molecular weight of heavy chain from l00 Kd to 90 Kd. When a limited digest of S1 with trypsin was further digested by chymotrypsin, 47 and 27 Kd peptides with essential light chain were unaffected, while both re-gulatory light chain and 23 Kd peptide notably disappeared. The limited digest of squid Sl with trypsin was trinitrophenylated with 2, 4, 6-trinitro benzene sulfonate either in the presence or absence of inorganic pyrophosphate and applied to a Bio-Gel P-100 column in the presence of SDS. It was found that the eluted substance containing trinitrophenyl group was composed from 47 Kd peptide.
On the basis of these observations, it was suggested that squid S1 heavy chain was made up of three peptides of 27, 47, and 23 Kd, arranging in the order from N-terminal to C-terminal end. It was also suggested that actin, regulatory light chain, and trinitrophenyl group binding sites were located on the link of 47 and 23 Kd peptides, on 23 Kd, and on 47 Kd peptide, respectively.抄録全体を表示PDF形式でダウンロード (5345K) -
山田 典彦, 新井 健一1987 年 53 巻 1 号 p. 77-83
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーA study was made on the superprecipitation of carp myosin B by measuring the change in turbidity induced by addition of ATP.
On addition of 0.2-0.4mm ATP, carp myosin B suspension in a medium containing 80-100mM KCl, 1mM MgCl2, 0.1mM CaCl2, 0.2-0.4mg/ml of myosin B, and 25mM Tris-maleate (pH 7.0), showed a typical change in turbidity at 25°C; that is, an early instant decrease for some time (clearing phase), followed by a rapid large increase leading to the maximum.
The turbidity changes, induced under above conditions, are favourable for estimation of superprecipitation of carp myosin B. The rate of superprecipitation was then demonstrated as the reciprocal value of the time required for half-maximal change in turbidity (t1/2)-1. It was thus found that (t1/2)-1 and turbidity change were in linear relations when the protein concentra-tion is lower than the approximate of 0.4mg/ml.抄録全体を表示PDF形式でダウンロード (480K) -
市川 寿, 西田 清義1987 年 53 巻 1 号 p. 85-91
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーBy using the myofibrils prepared from akazara scallop striated and smooth adductor muscles, dissociation of myosin regulatory light chains and the relation of the regulatory light chain con-tent to Mg-ATPase activity were investigated.
The regulatory light chain dissociation was found to occur more readily from the striated myofibrils than from the smooth ones. Moreover, it was confirmed that the complete removal of regulatory light chains was required for the complete loss of Ca-sensitivity of Mg-ATPase; and that the Mg-ATPase activity both in the presence and absence of Ca2+ changed in proportion to the regulatory light chain content. These results are very similar to those obtained with akazara myosin.抄録全体を表示PDF形式でダウンロード (3854K) -
尾島 孝男, 西田 清義1987 年 53 巻 1 号 p. 93-98
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn order to investigate whether the regulatory light chain (R-LC) removability depends on the R-LC or on desensitized myosin (DM) in the molluscan myosins, various hybridized myosins were prepared by combining of akazara DM with the R-LC of surf-clam foot and Atrina adductor myosins, and also of surf-clam DM with the R-LC of akazara scallop, ezo-giant scallop and Atrina adductor myosins. By using these myosins, it was observed that Ca-sensitivity of the Mg-ATPase activity of akazara intact myosin significantly decreased above 20°C, however, that of the akazara hybridized myosins with surf-clam and Atrina R-LCs practically unchanged. Whereas, the Ca-sensitivity of surf-clam myosin did not decrease above 25°C, but the surf-clam hybridized myosins with the akazara scallop and ezo-giant scallop R-LCs remarkably decreased.
It was also observed that the Ca2+ and Mg2+ concentrations for 50% dissociation of the R-LCs by heat (30°C)-treatment was estimated to be 4μM and 240μM for the akazara intact myosin, 0.2μM and 10μM for the akazara hybridized myosin with surf-clam R-LC, 0.2μM and 16μM for surf-clam intact myosin, and 3.7μM and 100μM for surf-clam hybridized myosin with akazara R-LC.
From these results, it is concluded that the difference in the affinity of R-LC to DM is de-pendent mainly on the R-LC rather than on the heavy chain.抄録全体を表示PDF形式でダウンロード (418K) -
牧之段 保夫, 北川 剛史, 豊原 治彦, 志水 寛1987 年 53 巻 1 号 p. 99-101
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーTo clarify whether heat-stable alkaline proteinase (HAP) relates to “Himodori” phenomena of Kamaboko, the effect of HAP of carp muscle on the property of horse-mackerel meat paste was examined. HAP clearly weakened the gel strength of Kamaboko at 65°C and the hydrolysis of protein was observed on the SDS-polyacrylamide gel electrophoretic pattern, although not clearly. These results seem to show that HAP is a possible factor inducing “Himodori” pheno-mena of Kamaboko gel.抄録全体を表示PDF形式でダウンロード (784K) -
塩見 一雄, 折井 真人, 山中 英明, 菊池 武昭1987 年 53 巻 1 号 p. 103-108
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn Dowex 50 column chromatography, arsenate, arsenite and methylarsonate (MAA) were quantitatively recovered in the unadsorbed fraction, dimethylarsinate (DMA) and arsenobetaine (AB) in the NH4OH fraction and arsenocholine (AC) in the HCI fraction. These arsenic compounds were subjected to HPLC using ion-exchangers and specifically monitored with an inductively coupled argon plasma emission spectrometer (ICP). Mutual separation of arsenate, arsenite, MAA and DMA was achieved on Nucleosil 10SB (anion exchanger) with 0.02M phosphate buffer (pH 7.0) and that of DMA, AB and AC on Nucleosil 10SA (cation exchanger) with 0.1M pyridineformate buffer (pH 3.1). A linear relationship between the peak height and the arsenic amount was obtained for each arsenic compound. Based on these results, a HPLC-ICP method combiped with the pre-treatment on Dowex 50 was postulated for the determination of the six arsenic compounds. By this HPLC-ICP method, it was found that the major arsenic compound in the shellfishes analyzed was AB regardless of their feeding habits. In the mid-gut gland of the carnivorous gastropod C. sauliae, AC was also detected as a minor component.抄録全体を表示PDF形式でダウンロード (463K) -
松浦 基, 新井 健一1987 年 53 巻 1 号 p. 109-118
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーMyosins from tilapia, carp, and rabbit muscles, were mixed with rabbit light-meromyosin (LMM) in a medium of 0.5M KCl (pH 7.5) and KCl concentration was diluted into 0.1M to form each filament and co-filament from myosin and LMM, respectively. Thus, the effect of LMM on the ultrastructure in electron micrographs and on some biochemical properties of these filaments were investigated. In general, when the myosin formed thick filaments and co-filaments with LMM bigger size of 290Å in width and 0, 72μm in length, the central bare zone was known to be formed. The actin-activation of myosin Mg-ATPase of these bipolar filaments occurred and specific activity was saturated by a small amount of actin. Moreover, such filaments and co-filaments as above were found to be hard to disassemble into mysoin and LMM (or its short fila-ments), from the change in light scattering intensity caused by treatment with KCl or ATP.
In particular, thick filaments of tilapia myosin formed in vitro, can be disassembled by a relatively low concentration of KCl or ATP, and the actin-activation of its Mg-ATPase was weak. However, LMM increases in the actin-activation of myosin Mg-ATPase by forming ATP or KCl resistant bipolar co-filaments with myosin.抄録全体を表示PDF形式でダウンロード (3471K) -
佐藤 秀一, 竹内 俊郎, 渡辺 武1987 年 53 巻 1 号 p. 119-124
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーFeeding experiments were conducted to investigate the requirement of Tilapia for vitamin E and to see whether or not the vitamin E requirement of Tilapia is increased by elevating dietary lipid levels.
No apparent deficiency symptom was induced in fish receiving diets of different levels of α-tocopherol with 5% of lipid, although fish fed with the tocopherol-free diet showed the lowest hepatosomatic index. The addition of more than 5mg of α-tocopherol per 100g diet completely prevented the appearance of this symptom. The concentration of α-tocopherol in the liver of fish receiving the tocopherol-free diet was lowest and increased as dietary tocopherol levels increased.
The fish receiving diets containing 10% and 15% fish oil methyl esters without tocopherol began to show low appetite and reduced growth from the 3rd week of feeding and were low in growth and feed conversion efficiency. The supplement of 50mg α-tocopherol to these diets effectively improved these conditions. Elevation of the dietary lipid levels from 5 to 15%. in the diet containing 50mg of α-tocopherol reduced the concentration of α-tocopherol in the whole body.
The results obtained in these experiments suggest that 5-10mg of α-tocopherol per 100g of diet containing 5% lipid should provide adequate protection in most practical Tilapia diets. The elevation of dietary lipid levels increased the tocopherol requirement of Tilapia, judging from the growth and mortality.抄録全体を表示PDF形式でダウンロード (440K) -
河野 迪子, 松居 隆, 清水 千秋1987 年 53 巻 1 号 p. 125-129
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThis study was conducted to examine the effect of supplementing chitin, chitosan, and cellulose in the diet on the growth of red sea bream, Japanese eel, and yellowtail. The growth rate of all fishes fed with the 10% chitin supplemented diet recorded the highest value indicating diet super-iority. The feed efficiency in the red sea bream and Japanese eel which were fed with the 10 chitin supplemented diet also recorded the highest values. All fishes fed with the chitosan sup-plemented diet had the lowest growth rates and feed efficiencies indicating that the inclusion of 10% chitosan in the diet must have inhibited the processes involved with the digestion, absorption, and assimilation of the basal diet. The growth rates and feed efficiencies of red sea bream and yellowtail fed with the 10% cellulose supplemented diet were slightly higher than the values recorded for the control diet.
Chitinase, chitosanase, and cellulase activity in the stomach of the three types of fishes which were fed with the aforesaid diet were measured and only chitinase activity was detected. The level of chitinase activity in the stomach of these three fishes were in proportion to the rate of growth when fed with the chitin supplemented diet. These results suggested that in fishes which possess high activity of chitinase in their digestive gland, chitin is digested and utilized.抄録全体を表示PDF形式でダウンロード (382K) -
河野 迪子, 松居 隆, 清水 千秋1987 年 53 巻 1 号 p. 131-136
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーChitinase was purified from the stomach of red sea bream Pagrus major by fractionations with ammonium sulfate, Sephadex G-100 gel filtration, DEAF-cellulose, CM-cellulose and hydro-xylapatite column chromatography. The purified enzyme showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be 46, 000. The isoelectric point was 8.3. The optimum temperature and pH were 50°C and 5.5, rerpectively. The activity was completely inhibited by Hg2+, strongly inhibited by Fe2+ and Sn2+, and slightly. activated by Cu2+. The products of hydrolysis of chitin with the enzyme were N-acetylglucosamine and N, N'-diacetylchitobiose.抄録全体を表示PDF形式でダウンロード (1177K) -
林 賢治, 山本 昭一1987 年 53 巻 1 号 p. 137-140
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe content and composition of alkyl glyceryl ethers of liver lipids of the gonatid squid Berryteuthis magister in the northwestern Pacific were investigated.
Weight of the livers of thirty specimens of this species accounted for 9.6±3.7% of total body weight; 35.2±15.6% of the liver was lipids. The liver lipids contained significant amounts of unsaponifiables (23.8±4.4%), consisting of 76.5±16.2% alkyl glyceryl ethers and 20.2±15.4% sterols. Alkyl glyceryl ethers originated from the constituent diacyl glyceryl ethers (46.5±17.8%) of the liver lipids. The relationship between the amounts (Y, g) of alkyl glyceryl ethers of liver and liver weight (X, g) was expressed by the following equation: Y=0.002+0.83X (r=0.966, P<0.05).
The component alkyl glyceryl ethers were found to consist mostly of saturates (77.8±3.6%) and monoenes (20.0±3.2%) of 14-20 carbon numbers for the alkyl moiety. The principal com-ponents found were: chimyl (16:0, 71.1±4.0%), selachyl (18: 1, 17.0±2.5%), and batyl (18:0, 3.5±1.5%) alcohols. It was concluded that the liver of the gonatid squid species was a good source of alkyl glyceryl ethers having chimyl alcohol as its predominant component. In addition, the component fatty acids of the liver lipids were discussed.抄録全体を表示PDF形式でダウンロード (329K) -
鶴田 新生, 上野 俊士郎, 大貝 政治, 山田 真知子1987 年 53 巻 1 号 p. 141-144
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe seasonal and horizontal distributions of Skeletonema costatum (GREV.) CLEVE were observed five times in the surface water of Yatsushiro Sea, Kumamoto and Kagoshima Prefectures, from October 1983 to November 1984. Numerically, S. costatum was by far the most important phytoplankton, with two maxima in February and in August. In terms of cell number, this species amounts to more than 50% of the total phytoplankton whenever the community density is more than 105 cells/l. The first maximum in February seems to be triggered by the release from zooplankton grazing pressure, since the high cell density of S. costatum and the high concentration of DIP were observed widely in Yatsushiro Sea. The second maximum in August seems to be attributed to the nutrients supplied through R. Kuma, since a marked horizontal gradient of S. costatum strongly related to those of salinity and DIP.抄録全体を表示PDF形式でダウンロード (297K) -
鶴田 新生, 大貝 政治, 上野 俊士郎, 山田 真知子1987 年 53 巻 1 号 p. 145-149
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーThe study dealt with the influence of nutrients on the growth of Skeletonema costatum, which was collected from the Yatsushiro Sea, Dokai Bay and Hibiki Nada, in the laboratory cultures. The cultures in the standard artificial medium (ASP12) and its variations without each nutrient showed that the growth of the three types decreased with the lack of inorganic nitrogen, silicate, borate and vitamin B12, and the tendency of the growth of the three types caused the same results essentially. But, the relation between the growth of the three types and the concentrations of the nitrate nitrogen and silicate in particular, among the nutrients, was found to show a remarkable variations in each type.
Therefore, it was considered that the three types of S. costatum were ecotypes adapted to the difference of water quality at the collecting stations, and this produced the same results as the influence of chlorinity in the previous paper.抄録全体を表示PDF形式でダウンロード (435K) -
川田 倫夫, 柴田 猛1987 年 53 巻 1 号 p. 151-157
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーIn order to obtain further information on the regulation of the glycogen-degradating meta-holism in fish skeletal muscle, the activation by calcium ion and other substances, autocatatytic reaction and limited proteolysis of carp muscle phosphorylase kinase (EC.2.7.1.38) were studied.
It was shown that the activation pattern of the carp enzyme by calcium ion and calmodulin was the same as that of rabbit enzyme, suggesting the presence of calcium regulation mechanism in the fish muscle.
The activation by glycogen of the fish enzyme was very low; and also the extent of activation by the procedures of auto-catalytic reaction and limited proteolysis of the fish enzyme were less than that of rabbit enzyme. That the fish enzyme obtained by the limited proteolysis with trypsin lost the response to calcium sensibility and γ-subunit of the enzyme was susceptible to the limited proteolysis, differed from that of rabbit enzyme.抄録全体を表示PDF形式でダウンロード (2313K) -
金 鐘培, 村田 道代, 坂口 守彦1987 年 53 巻 1 号 p. 159-164
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーA method to differentiate between frozen-thawed and unfrozen fillets of yellowtail Seriola quinqueradiata was investigated by a combination of Torrymether readings (TMR) and K values (KV). Fresh fillets before storage and fillets held in ice for about a day showed an average TMR of approximately 11. KV for corresponding fillets were less than 1 and about 6%, respectively. TMR for frozen fillets stored at -20°C for about a day prior to thaw and unfrozen fillets held in ice for 18 days were both 0. The frozen-thawed fillets had relatively low KV, while the unfrozen ones had a considerably high level (approximately 3 vs. 50%), suggesting that the former fillets can be differentiated from the latter by measuring the TMR and KV. Thawed fillets previously frozen only in liquid nitrogen and those frozen by this and subsequently stored at -20°C for about a day also showed a TMR of 0. TMR of frozen-thawed fillets measured on the bone as well as on the skin side were much lower than those of the unfrozen fillets. A possibility to dif-ferentiate frozen (-20°C for a day)-thawed from unfrozen fillets by a combination of visual ob-servation of the skin color and the TMR or KV measurement was also indicated.抄録全体を表示PDF形式でダウンロード (2016K) -
渡辺 悦生, 高井 陸雄, 田中 宗彦1987 年 53 巻 1 号 p. 165
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (100K) -
浜野 龍夫, 松浦 修平1987 年 53 巻 1 号 p. 167
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (92K) -
原田 勝彦, 福田 一弥1987 年 53 巻 1 号 p. 169
発行日: 1987年
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (110K)
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