巻号一覧
54 巻, 11 号
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三谷 勇1988 年 54 巻 11 号 p. 1859-1865
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーIn order to clarity food habits of Japanese anchovy in the Shirasu (anchovy larvae) fishing ground within Sagami Bay, gut contents were analysed during the period of July through October, 1984. This period was the major fishing season for Shirasu.
The prey organisms in the guts consisted of 7 kinds of zooplankton. Copepoda were the most dominant prey items, Among others, Oithona comprised 67 numerical percentage of total prey organisms detected in the guts. Food preference of larval and juvenile anchovies was found to change with increase in lengths: TL 20-25mm size-class chiefly fed on O. davisae and Evadne, 26-30mm size-class on Paracalanidae and Penilia in addition to the former two items, and anchovy over 31mm fed on Oncaea, Microsetella, Bivalvia larvae and so on. Seasonal abundance of O. davisae in anchovy guts changed with the density of O. davisae in the bottom layer of the fishing ground.
According to change of food preference with growth, anchovy probably moved from one region, where O. davisae was abundant, to the other region where another zooplankton was distribut-ed, so that the school seemed to be composed of almost the same size of anchovy.抄録全体を表示PDF形式でダウンロード (486K) -
田畑 和男, 五利江 重昭1988 年 54 巻 11 号 p. 1867-1872
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe following results were obtained from the experiments to find optimum conditions for induction of gynogenetic diploids in hirame Paralichthys olivaceus by suppression of the lst cleavage, and to clarify their survival and growth rates. Induction of gynogenetic diploids by suppression of the lst cleavage was achieved by adding hydrostatic pressure of 650kg/cm2 for a duration of 6min at 60min after insemination, but the induction rate was low (2.7-5.3%). Genotypes of gynog-enetic diploids induced by suppression of the lst cleavage from female whose genotype in TDH isozyme was heterozygous, separated into 2 homozygous types. Since genotype of IDH isozyme in gynogenetic diploids induced by retention of the 2nd meiosis was heterozygous, induction of homozygous hirame has been easily identified. However, thier survival rates were very low, growth rate was not good, and coefficient of variations of total length was relatively large in gy-nogenetic diploids.抄録全体を表示PDF形式でダウンロード (1311K) -
于 建平, 日野 明徳, 平野 礼次郎, 平山 和次1988 年 54 巻 11 号 p. 1873-1880
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーOut of 31 bacterial strains isolated from culture tanks of the rotifer Brachionus plicatilis, only 8 strains which were vitamin B12 producers supported the growth of germ-free rotifer when they were added into yeast suspension. Of the 6 strains which were taxonomically examined, they were identified as Pseudomonas. Their vitamin B12 productivity was measured by Euglena bioassay method. The concentrations of vitamin B12 in the bacterial suspension were measured daily and the higbest levels of B12 were estimated to be 1.2-16.5ng/ml during 8 day culture. Most (98.1-99.3%) of the vitamin B12 which was produced accumulated inside the bacterial cells.
The rotifer growths were tested in batch cultures under germ-free condition, provided with vitamin B12-producing bacteria as the sole food source, and with these bacteria as a complement of Nannochloropsis oculata or baker's yeast Saccharompces cerevisiae. The rotifer showed rapid growth when vitamin B12 producing bacterial cells were supplied in a range of 107-1011cells/ml.
The results imply that in mass production tank of the rotifer vitamin B12-producing bacteria might play an important role as vitamin B12 supplier.抄録全体を表示PDF形式でダウンロード (504K) -
森友 忠昭, 浅川 洋美, 中野 雅功, 渡辺 翼1988 年 54 巻 11 号 p. 1881-1887
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーWe tried to cultivate fish liver cells using the embryonated eggs of Yamame, landlocked form of masu salmon, Oncorhynchius masou. A continuous cell line designated YEL-13 was established and has been subcultured more than 120 times over the period of 3 years. YEL-13 cells grow in the manner of contact inhibited monolayer and consisted of epithelial cells and a few fibrobrastic cells. Electron microscopically, most of the cells had a large and round nucleus and well-develop-ed rough surfaced endoplasmic reticulum in cytoplasm and smooth cell surface with poorly de-veloped microvilli. Chromosme number of YEL-13 ranged widely and most of them were be-tween 50 and 83. The cells with 56 chromosomes occupied 24% of those examined. YEL-13 cells have the permissible temperature range of 15-20°C for growth. The cell line has been producing and relaesing a Yamame serum protein constantly which is one of the main functions of liver parenchymal cells.抄録全体を表示PDF形式でダウンロード (915K) -
藤岡 康弘, 伏木 省三1988 年 54 巻 11 号 p. 1889-1897
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーDownstream migration and body silvering of landlocked biwa salmon Oncorhynchus rhodurus indigenous to Lake Biwa were observed in Lake Biwa and the rivers which flow to the lake in central Japan from 1983 to 1986. Underyearling biwa salmon inhabited the gently flowing shallows of the river shore in April, and moved into the riffle in May, when they began to migrate downstream into the lake. Downstream migration of the juvenile fish (5 to 7cm in standard length) was mainly observed from the middle of May to the end of June. The downstream migration seems to be related with rainfall, as the number of the fish moved into the lake increased on the day and/or the next day of rain, Although the sex ratio of the juvenile fish in the river in May was equal, the ratio of male became greater from June to July. Juvenile fish were scarcely observed in the river after July; in autumn only a small number of residual precocious mature males inhabited the river. After descending into the lake, they inhabited the offshore zone of the lake deeper than 20m. There was a significant difference in the slopes in the growth lines between the juvenile fish in the river and in the lake; the fish in the river grew faster than those in the lake. Body silvering of the juvenile fish began from the end of May in the river, and parr marks were scarcely observed in some of the fishes just after descending into the lake. Although parr marks completely disappeared in the fish greater than 10cm in standard length after September, even those fish did not show in-tense darkening at outer extremities of dorsal fin. The size and age of biwa salmon migrating downstream were similar to those of chum and pink salmon, and different from those of closely related amago and masu salmon. The process of body silvering in biwa salmon seems to be related to the downstream migratory behavior, since they are known not to acquire seawater adapt-ability as underyearlings抄録全体を表示PDF形式でダウンロード (1620K) -
中田 英昭, 竹内 博治, 平野 敏行1988 年 54 巻 11 号 p. 1899-1906
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーA field experiment with drifting hoop nets was carried out in the coastal water of the Kuroshio during 6-12 August 1977 in order to collect the small fish associated with drift algae. Two types of drifting hoop nets composed of 5 large (0.83m3) and small (0.22m3) hoop nets were released about 50 miles off Shikoku on 6 August and tracked; each type was retrieved twice during the tracking to catch the fish in the nets. The juvenile and young fish of 9 species (7 families) were collected including Arothran firmamentum (467 ind.), Abudefduf vaigiensis (71 ind.), Thamnaconus modestus (70 ind.) and Decapterus maruadsi (8 ind.). The greater part of the fish were collected with 7mm mesh large nets. T. modestus and D. maruadsi were mostly collected at a depth of about 13m in contrast to A. vaigiensis and A. firmamentum which were collected at the surface. Body lengths (ranging 80-108mm in BL) of T. modestus were somewhat larger than previously reported. The appearance of young A. firmamentum suggests that they possibly migrated in and around the Kuroshio Current off Shikoku during the period of this experiment.抄録全体を表示PDF形式でダウンロード (544K) -
松宮 義晴, 服部 唯史1988 年 54 巻 11 号 p. 1907-1911
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThis study is to propose that comparative assessment is performed on the habitability of 0-age red sea breams as expressed in terms of unified indices. Relative condition index y1 and density index y2 are calculated using degree of fatness and distribution density in red sea bream caught by the fixed station. Habitability index y3 is determined to y1+y2. The habitability at two bays of Hirado Island is assessed according to three kinds of indices. The inner bay areas in both the bays are ranked high in good agreement with past ecological studies. A multiple regression analysis is undertaken using y1, y2 and y3 as criterion variables, and bottom materials, organic matter and feed biomass as explanatory variables. A considerable correlation is observed between three indices and the feed biomass variables, especially gammarids as a main food for red sea bream young.抄録全体を表示PDF形式でダウンロード (338K) -
山本 勝太郎, 平石 智徳, 小島 隆人1988 年 54 巻 11 号 p. 1913-1918
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーNet cages become inevitably encrusted with marine organisms in scallop culture. The darg of the net cage composed of 10 compartments (0.5m in diameter and 2m high, covered with 0.8mm polyethylene mono-filament net of mesh size 4.0cm) encrusted with marine organisms is still not sufficiently clear. In this study, we measured the drag of three net cages (named cage A, cage B and unused cage) with different encrusted conditions of marine organisms in an experimental tank. The cage A was suspended by a main rope in an actual scallop culture facility for 3.5 months in winter season and marine organisms weighed 0.62kg in water without scallops. The cage B was used by a scallop culturist over a period of 14 months in the same facility, and its overall weight in the water consisted of 1.6kg of cage, 5.5kg of 100 scallops grown up to average size of 120mm, and 2.4kg of marine organisms. The unused cage was an empty new cage.
The experimental results were drawn in CD-Re diagrams (CD: drag coefficient, Re: Reynolds number) For each of the three cages the change of CD to Re was small within the experimental range of 5.0×104<Re<3.0×105. The mean value of CD was 1.2 for cage A, 1.9 for cage B and 0.39 for the unused cage when set in normal to the water flow and 1.6, 1.8 and 1.4 when set in parallel, respectively.抄録全体を表示PDF形式でダウンロード (1031K) -
小川 瑞穂, 福地 光男1988 年 54 巻 11 号 p. 1919-1922
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe structures of the kidncys and urinary bladders of four Antarctic teleosts, Pagothenia borchgrevinki, Trematomus bernacchii, Trematomus hansoni and Austrolycichthys brachycephalus, were compared by light microscopy. The kidney of A. brachycephalus is glomerular; the other three species studied have aglomerular kidneys.
The epithelium of urinary bladder in P. borchgrevinki and T, bernacchii, is composed of colum-nar cells in the areas near the collecting tubule but gradually changes to cuboidal cells which line most of the bladder. The entire bladder epithelium of T. hansoni is composed of simple cuboidal cells while the bladder epithelium of A. brachycephalus shows intermediate type between the other two groups.
These morphological differences in urinary bladder epithelia of Antarctic teleosts may reflect adaptations to diluted sea water during the thawing season.抄録全体を表示PDF形式でダウンロード (1487K) -
右田 清治1988 年 54 巻 11 号 p. 1923-1927
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーGrateloupia filiclna (Lamouroux) C. Agardh, Rhodophyta, Cryptonemiales, is one of the edible marine algae used as “seaweed salad” in Japan. The carpospores and tetraspores of this alga germinate into circular crusts from which erect thalli grow up and develop to Grateloupia-thalli. When the crusts were cut into many fragments in a Petri-dish, cut fragments regenerated into similar new crusts. The crusts grew to about 1.2mm in diameter after 35 days culture. The regeneration of crusts by cutting could be repeated throughout the year, making propagation of crusts very easy. For cultivation in the sea, the cut fragments of crusts were scattered on oyster shells and synthetic twine of Nori-net and cultured for about one month in the laboratory. The substrata with regenerated crusts were transferred into the sea from October to December. Many erect thalli grew up from the substrata and developed into about 15cm in length 2 months after transfer into the sea.抄録全体を表示PDF形式でダウンロード (2777K) -
大富 潤, 清水 誠, J. A. Martinez Vergara1988 年 54 巻 11 号 p. 1929-1933
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーSpawning season of the Japanese mantis shrimp Oratosquilla oratoria in Tokyo Bay was ex-amined from seasonal changes in percentage occurrence of mature females and in female gonad index. A total of 15, 871 specimens was sampled in Tokyo Bay from April to December in 1982 and from August 1986 to September 1987.
Analysis showed that spawning season lasted from April to August. Percentage occurrence of mature females during the spawning season showed two peaks, the first one from April to May, and the second from July to August. The first peak was caused by the maturation of females older than 2 years with body lengths above 10cm. In July or August, 1 year old females with body lengths above 8cm matured and contributed to the second peak. The result of the present study showed that the age of maturation of the mantis shrimp in recent years has become rather earlier compared with that in the 1950s in Tokyo Bay.抄録全体を表示PDF形式でダウンロード (370K) -
大富 潤, 清水 誠1988 年 54 巻 11 号 p. 1935-1940
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーMean body lengths of age groups by sex of the Japanese mantis shrimp Oratosquilla oratoria in Tokyo Bay were determined by 2, 423 females and 2, 293 males using size-frequency analysis method. A growth curve was fitted to length at age data and the life span was examined. It was found that the recruitment period started in November with a body length around 5cm and com-pleted in June of the following year. The mode of age O group during the recruitment period was not used in further interpretations, because the continuous addition of newly recruited individuals was found to lower its value. After recruitment completion, it was found that the growth of the mantis shrimp has seasonal oscillation with the highest growth rate from late summer to late autumn. Females showed higher growth oscillation than males. Growth of the mantis shrimp was best expressed by the Pauly and Gaschütz equation as Lt=16.12{1-exp[-0.395(t+2.667)-(0.486/2π)sin 2π(t-0.323)]} for females and Lt=21.51{1-exp[-0.264(1+2.473)-(0.232/2π)sin 2π(t-0.281)]}for males, where Lt is body length in cm and t is age in months. It seems that most of the mantis shrimp in Tokyo Bay died during age 2.抄録全体を表示PDF形式でダウンロード (336K) -
内田 有恆, 嶋田 敦弘, 石田 祐三郎1988 年 54 巻 11 号 p. 1941-1945
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe ether-soluble fraction of the dinoflagellate Peridinium bipes showed antibacterial and antialgal activities. The main components of the antibiotic substances were suggested to be unsaturated fatty acids and photooxidized chlorophyll c.抄録全体を表示PDF形式でダウンロード (1171K) -
冨岡 和子, 遠藤 金次1988 年 54 巻 11 号 p. 1947-1951
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーIn order to clarify the mechanism of the inhibitory effect of some additives on the enzymatic decomposition of 5'-inosinic acid (IMP)in fish muscle, the effect of sodium chloride and glycerine was examined on the 5'-nucleotidase and acid phosphatase in various fish muscle extracts.
The pH-activity curves observed for IMP-decomposition in fish muscle extract varied with the different fish species and their pattern could be well explained by the activity ratios of 5'-nucle-otidase to acid phosphatase. It was therefore confirmed that IMP is decomposed in fish muscle by the action of a 5'-nucleotidase and an acid phosphatase.
These enzymes were suppressed by addition of sodium chloride, in a competitive inhibitory manner. The apparent Ki values for 5'-nucleotidase and acid phosphatase of 8 species of fish examined, closely resembled each other, and were calculated to be 0.111-0.161M and 0.070-0.142M, respectively. It was also found that both enzymes were suppressed by addition of glycerine in the same manner. The apparent Ki values for 5'-nucleotidase and acid phosphatase of these fishes, regardless of difference of species of fish, were calculated to be 4.02-6.31M and 1.09-1.67M, respectively.抄録全体を表示PDF形式でダウンロード (354K) -
李 鍾壽, タンゲン カール, ダール アイナール, ホフガド ピーター, 安元 健1988 年 54 巻 11 号 p. 1953-1957
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーMussels infested with diarrhetic shellfish toxins were collected for toxin analyses at Arendal on the south coast and at Sogndal in Sognefiord of Norway. The Arendal mussels contained okadaic acid as the principal toxin, while the Sogndal mussels contained dinophysistoxin-1 as the major toxin and yessotoxin as the minor toxin. These results provide the first evidence for mul-tiple toxin profiles in mussels from European country. Chromatographic and spectrometric methods for determining microquantities of the toxins were established.抄録全体を表示PDF形式でダウンロード (347K) -
中山 照雄, 常川 正則, 加納 哲, 丹羽 栄二1988 年 54 巻 11 号 p. 1959-1964
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe steady and dynamic shear rate behaviors of carp actomyosin solution were measured during incubation at 40°C. The maximum steady shear viscosity was observed around 35 min, while the maximum dynamic rigidity was observed around 2h. The hysteresis loop area increased for 2h, and thereafter it remained almost unchanged till 6h where the dynamic rigidity decreased. Therefore we deduce that during the incubation, the network structure would develop also in the carp actomyosin solution as in the hen or rabbit myosin solution, but the network structure of carp actomyosin solution would be so weak as to be broken down even during the repeated measurements of dynamic behaviors.
The actomyosin solution at pH 5.8 exhibited the lowest viscoelasticity. The solution at pH 6.8 exhibited the highest dynamic rigidity. The solution at pH 7.8 exhibited the highest stress in the shear rate scan measurement. These results were explained by the fact that the actomyosin solution at pH 5.8 existed near the isoelectric point, and the network structure at pH 6.8 would be different from the network structure at pH 8.8.
In the temperature scanning experiment the dynamic rigidity began to increase around 40°C the maximum was observed around 61°C, The peak value around 61°C was high at pH 5.8 and pH 6.8, and low at pH 7.8.抄録全体を表示PDF形式でダウンロード (481K) -
森下 達雄, 宇野 和明, 松本 好央, 高橋 喬1988 年 54 巻 11 号 p. 1965-1970
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーDifferences in the proximate compositions were investigated among the red sea breams which were cultured at various localities or cultured by different methods, and a comparison was also made between these fishes and wild ones. There were no appreciable differences in the crude protein and ash contents among the cultured fishes from various localities. The lipid content was higher in the ordinary meast of fatty fish and was two times higher than the lean meat. On the other hand, the differences of lipid contents between these cultured fishes and wild ones were obvious; the contents in cultured fishes, even in the lean fishes, were 3.6 times higher than those in wild ones. There was little difference between the fishes fed on a fresh bait and those on a moist pellet except that the fishes cultured by feeding a moist pellt had lower lipid contents in the viscera. The lipid contents of fishes cultured at marifarm were lower, in all regional meats of the yearling fish (0 age fish), than those of the net cages, but were unexpectedly higher in the dorsal and ventral meats in one year old fishes.抄録全体を表示PDF形式でダウンロード (421K) -
中川 孝之, 永山 文男1988 年 54 巻 11 号 p. 1971-1974
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーExtractability of glycolytic enzymes from the ordinary muscle of red sea bream. Pacific mackerel and carp was examined in a range of 0-0.3M KCl (pH 6.8-7.1). The amount of extracted proteins increased w ?? th an increase of KCl concentration up to 0.1M, irrespective of fish species. The dependency on KCl concentration of the extractability of glycolytic enzymes such as aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucosephosphate isomerase (GPI) was roughly comparable to that of total sarcoplasmic proteins. Sarcoplasmic proteins, as well as ALD, GAPDH and GPI, of Pacific mackerel were effectively extracted with 0.3% NaHCO3-containing 0.15% NaCl, 0.15M KCl, or a phosphate buffer (pH 7.5, I=0.05). The amounts of extracted ALD, GAPDH and GPI with 0.3% NaHCO3-containing 0.15% NaCl were 1.5-4 times with that of distilled water. On the other hand, most of phosphoglycerate kinase and enolase was extracted in the absence of KCl.抄録全体を表示PDF形式でダウンロード (1104K) -
丹羽 栄二, 陳 恩笙, 加納 哲, 中山 照雄1988 年 54 巻 11 号 p. 1975-1979
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーEffect of temperature on the state of water within the kamaboko was examined in order to clarify the mechanism of temperature-dependence of its elasticity. Upon raising measuring temperature, the length of the kamaboko was almost unchanged and the weight was slightly decreased. The electrostatic capacity and the expressible water were increased. In the differential IR spectrum of the kamaboko, a band due to free water was strengthened. On the other hand, it was microscopically observed that the network structure within the kamaboko was broken into pieces with raising the temperature.
Based on the above results, there arose a possibility of the conversion of bound water to free water within the kamaboko caused the thermal decrease in the elasticity.抄録全体を表示PDF形式でダウンロード (1105K) -
甲斐 徳久, 上田 正, 武田 靖昭, 片岡 昭吉1988 年 54 巻 11 号 p. 1981-1985
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe levels of mercury and selenium in thirty blood specimens of two species of tunas, i.e., eighteen individuals of yellow fin Thunnus albacares and twelve individuals of big-eye Tlaunnus obesus, were presented.
Mercury level and the ratio of methyl mercury content (MeHg) to total mercury content (T-Hg) in the blood were lowest in yellow fin and highest in big-eye, respectively, as compared with those in the other tissues and organs previously reported. Both the mercury contents (T-Hg and McHg)in big-eye were extremely high compared with those in yellow fin, and those contents in both species did not tend to increase with the growth of the fish.
Selenium content (Se) in the blood was highest in all tissues and organs, and there was no significant difference in the high selenium levels between both species.
The molar ratio(Se/Hg)of Se to T-Hg in the blood was also highest in all tissues and organs, and correlated negatively with T-Hg in the olood of both species. From the correlation, it was observed that yellow fin and big-eye belong to the one region (low T-Hg and large Se/Hg) and to the other (high T-Hg and small Se/Hg), respectively.抄録全体を表示PDF形式でダウンロード (255K) -
浜田 盛承, 熊谷 洋1988 年 54 巻 11 号 p. 1987-1992
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーA chemical analysis of the scale of sardine Sardinops melanosticta was made. The major components were found to be ash and a nitrogen-containing substance, and ash and nitrogen contents were 53.16% and 7.38%, respectively, on the basis of dried scale. Total sugars and crude lipid, both of which were low in content, were 0.21% and 0.08%, respectively. Calcium, phosphorus and magnesium in 100g crude ash were 39.39%, 21.08% and 2.08%, respectively. Sodium, potassium and some heavy metals were extremely low in content. Molar ratio of calcium tophosphorus was about 3 to 2. The ratios of hydroxyproline to protein and of nitrogen to protein in the boiling-water extract of scale, together with the SDS polyacrylamide gel electrophoretic pattern of the acetic acid extract of scale, have proved that the nitrogen-containing substance was mostly a collagen. The collagen content was calculated to be about 43%. The amounts of both ash and collagen went up to 96%. This scale collagen was assumed to be type I, and the migration rate of α1 subunit of the collagen was almost the same with that of calf skin type I collagen, butthat of α2 subunit was slower than that of calf collagen.抄録全体を表示PDF形式でダウンロード (1070K) -
松山 博子, 中尾 実樹, 矢野 友紀1988 年 54 巻 11 号 p. 1993-1996
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーIn order to examine species compatibilities of antibody and complement, hemolytic reactions were carried out adopting sheep erythrocytes sensitized with carp, tilapia, yellowtail, porgy or rabbit antibody in combination with sera of rainbow trout, ayu, carp, tilapia, porgy, yellowtail or guinea pig. None of the fish sera were potentiated by rabbit lgM, and the guinea pig comptement did not react with fish antibodies. Some compatibilities were observed between antiboby and complement of different fish species; rainbow trout, ayu and porgy complement were potentiated by porgy, carp and tilapia antibody, respectively.
The hemolytic activity of fish sera was lost by carrageenan-, ammonia-and heat-treatment, and the combination of ammonia-treated serum with carrageenan-treated serum (Cn) or heat-treated serum resulted in restoration of the hemolytic activity. This indicates that these fish sera contain the complement components C1, C4 and C2.
Hemolytic intermediate complex, EACl, was incubated with Cn (serum deficient in C1) from different species to examine the species compatibility between C1 and C4. As a result, no compatibility was observed between the guinea pig and fish. Furthermore, in the majority of the combinations of C1 and Cn of different fishes, hemolysis were not induced; interspecies compatibilities were seen in only two combinations (porgy C1+tilapia Cn and tilapia C1+porgy Cn).抄録全体を表示PDF形式でダウンロード (334K) -
矢野 友紀, 松山 博子, 中尾 実樹1988 年 54 巻 11 号 p. 1997-2000
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーA hemolytic intermediate complex EAC1, 4, 2 was prepared by incubating carp EAC1, 4 with hydrazine-treated carp serum, which is deficient in C4 and C3, at 0°C for 20 min. The difference between EAC1, 4, 2 and EAC1, 4 was shown by tests with inactivated carp sera. The former was hemolyzed by carrageenan-treated serum (Cn), ammonia-treated serum (Am) and heat-treated serum (H), whereas the latter was hemolyzed by Cn and Am, but not by H.
EAC1, 4, 2 was unstable. Its half-life was about 15 min at 20°C and about 2.5h at 0°C. Addition of Ca2+ to the buffer was not effective in preventing the deterioration of this complex.
The reaction between EAC1, 4 and Cy' (a mixture of complement components other than C1 and C4) proceeded only in the presence of Mg2+, whereas the reaction of EAC1, 4, 2 with Cy'proceeded without Mg2+. This indicates that the action of C2 requires Mg2+ while C3 acts without Mg2+.抄録全体を表示PDF形式でダウンロード (248K) -
黄 登福, 野口 玉雄, 荒川 修, 阿部 宗明, 橋本 周久1988 年 54 巻 11 号 p. 2001-2008
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーForty-five specimens of four puffer species landed at fish markets in Taiwan were assayed for anatomical distribution of toxicity. “Dokusabafugu” Lagocephalus lunaris contained moderate amounts of toxin in almost all tissues, and the highest toxicity scores were 460MU/g ovary, 380MU/g intestine, and 100MU/g muscle. In “komondamashi” Fugu alboplumbeus, the hlghest scores were 180MU/g ovary and 120MU/g livei. “Takifugu” F. oblongus, whose toxicity data have not been available thus far, was strongly toxic, the highest scores being 2, 000MU/g liver and 770MU/g ovary. “Shippofugu” Amblyrhynchotes hypselogenion whose toxicity data were lacking was also strongly toxic. The highest scores were 750MU/g ovary and 720MU/g liver.
The toxin was partially purified from the toxic liver of each species by ultrafiltration using a Diaflo YM-2 membrane, followed by chromatography on Bio-Gei P-2. Analyses by electrophoresis, TLC, HPLC and GC-MS showed that the toxin from each species was composed of tetro-dotoxin, anhydrotehodotoxin and 4-epitetrodotoxin. In addition, a tetrodonic acid-like toxin was detected through the four species.抄録全体を表示PDF形式でダウンロード (541K) -
岩見 史郎, 新井 健一1988 年 54 巻 11 号 p. 2009-2017
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーDenaturation of actin portion of carp myosin B induced by three polymerized phosphates (sodium pyrophosphate (PPi), sodium tripolyphosphate (TPi), sodium polyphosphate (PolyPi)) was investigated.
When 1mM PPi or TPi, or 0.03% PolyPi was added to myosin B in a medium consisting of 0.3-0.7M KCl, 40mM Tris-maleate (pH 7.0) and 0-5.0mM MgCl2 a change in various ATPase activities, an increase of 50mM KCl soluble actin, and liberation of inorganic phosphate (Pi) were measured as a function of time at 10°C.
Comparison of the rates and extents of actin release from myosin B and of Pi liberation from polymerized phosphate suggested that production of PPi through hydrolysis of TPi and PolyPi by myosin triphosphatase caused therelease of denatured actin from myosin B into 50mM KCL medium. As the KCL concentration of myosin B solution was increased from 0.3 to 0.7M, the denaturation reaction of actin in myosin B was accerelated.抄録全体を表示PDF形式でダウンロード (625K) -
岩見 史郎, 新井 健一1988 年 54 巻 11 号 p. 2019-2027
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーInorganic pyrophosphate(PPi)and MgCl2 were mixed with carp myosin B (MB) to give the final concentration of each 1mM, in the co-presence of 0.3, 0.5, or 0.7 M KCl and 40mM Tris buffer (pH 7.0).
Changes in Ca-, Mg-, and EDTA-ATPase activities, ATP sensitivity of viscosity, and uitracentrifugal fractionation behavior of MB induced by treatment with PPi-MgCl2 and KCl were investigated with a lapse of time at 10°C.
After the treatment with PPi-MgCl2 and KCl for 9h, ultracentrifugal sedimentation pattern and gel filtration profile on Sepharose Cl-4B followed by SDS-polyacrylamide gel electrophoretic analysis of MB were also examined.
The results showed that a large portion of MB was dissociated into actin and myosin upon addition of PPi-MgCl2 in the presence of KCl. As the treatment time prolonged as well as KCl concentration increased, actin dissociated from MB gradually increased and denatured to lose its binding ability with myosin.抄録全体を表示PDF形式でダウンロード (1350K) -
池田 静徳, 石橋 泰典, 村田 修, 那須 敏朗, 原田 輝雄1988 年 54 巻 11 号 p. 2029-2035
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーThe qualitative requirements and deficiency signs of water-soluble vitamins were studied in Japanese parrot fish Oplegnathus faciatus having average weight of 2.5g.
The Japanese parrot fish showed a loss of appetite and poor growth when the purified testd iets deficient in B6, pantothenic acid, B1, B2, C, choline and nicotinic acid were fed. The deficiency of these vitamins except choline and nicotinic acid resulted in a high mortality.
On the other hand, the fish fed the diets deficient in B12, biotin, folic acid and inositol showed only slightly poor growth than the control fish fed the complete diet.抄録全体を表示PDF形式でダウンロード (477K) -
岡田 猛, 猪上 徳雄, 信濃 晴雄1988 年 54 巻 11 号 p. 2037-2042
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーTo study the influence of KCl concentrations on denaturation of carp F-actin, F-actin solutions were stored at 3°C in each KCl solution of 0.1, 0.6 and 1.0M. Viscosity, total and reactive SH groups, and bound nucleotide were measured during storage, and the protective effects of K2-Na1 phosphate buffer, sodium glutamate and ATP on salt denaturation were investigated.
The reduced viscosity and the bound nucleotide drastically decreased in the 0.6 and 1.0M KCl solutions, and in the case of the 0.6M KCl solution, the decrease in the reduced viscosity occurred within 3-5h. The total SH groups gradually decreased during storage and the reactive SH groups increased by 2 mol SH/mol of G-actin within 20h after introduction into the 0.6M KCl solution. Three additives, such as ATP, phosphate buffer and sodium glutamate showed protective effects on denaturation and the appearance of latent SH groups was prevented in these cases. In the case of the synthetic actomyosin, reconstituted by mixing myosin and F-actin which were stored separately in 0.6M KCl solutions, actin-activated Mg-ATPase activity decreased during storage. However, myosin Ca-ATPase activity (in the presence of 0.5M KCl) remained at its original value.
The above findings show that the structure of carp F-actin is easily changed from its fila-mentous state to small-sized actin or aggregations under higher KCl concentrations, the release of bound nucleotide from actin occurs, and that the latent SH groups of actin molecules appear as a result of denaturation.抄録全体を表示PDF形式でダウンロード (647K) -
山羽 悦郎, 松岡 誠, 山崎 文雄1988 年 54 巻 11 号 p. 2043
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (1070K) -
上田 高嘉, 佐藤 良三, 小林 仁道1988 年 54 巻 11 号 p. 2045
発行日: 1988/11/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (132K)
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