巻号一覧
55 巻, 10 号
選択された号の論文の28件中1～28を表示しています
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上田 幸男, 城 泰彦1989 年 55 巻 10 号 p. 1699-1702
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーGrowth, life span and spawning season of the oval squid Sepioteuthis lessoniana were studied in the outer waters adjacent to the Kii Channel, Japan. The spawning season was estimated during June to September. The von Bertalanffy's equations were applied to monthly size compositions. They were Lt=23.2[1-exp{-0.354(t+0.044)}] for female and Lt=25.7[1-exp{-0.408(t-0.063)}] for male, where Lt was mean mantle length (cm) at t months after hatching in August. These growth rates were much larger than those observed in aquaria. The life span was one year: S. lessoniana hatched out in summer and died just after spawning in next summer.抄録全体を表示PDF形式でダウンロード (320K) -
松村 靖治1989 年 55 巻 10 号 p. 1703-1706
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe catch of the young tuna Thunnus orientalis shows wide fluctuation by the year in waters around the Tsushima Islands. They changed from 150t to 2076t during the period from 1975 to 1987. Effects of four factors on the fluctuation were recognized in the present studies by the use of multiple regression analysis. The factors are as follows: the catch of the young tuna in Kochi Prefecture (X1), volume transport of the Tsushima current (X2), thermal gradient in western waters of the Tsushima Islands (X4), surface temperature at Meshima (X5). The relation between the catch (Y) and the factors is as follows:
Y=-4215.5+94.1X1+125.1X2-181.2X4+169.8X5 The regression is significant at 1% level. The factors are considered to have following meaning; X1 is the abundance of recruitment, X2 is the environment helping migration of the young tuna to the fishing ground, X4 and X5 are the conditions affecting fishing ground formation. It is considered that the catch in next fishing season could be forecast by the equation.抄録全体を表示PDF形式でダウンロード (314K) -
長谷川 英一, 小林 裕1989 年 55 巻 10 号 p. 1707-1714
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーIsoilluminance lines produced by two underwater lamps were described with relation to the displacement of lights by current, the extinction of lights, the arrangement of lights and the reflection at water surface.
Under the condition of this irradiance, the trace of swimming behavior of fish was estimated by the method of computor simulation, and was described by X-Y plotter.
The result suggests that it is a reasonable motion for the fish to swim lazily or zigzag under the fish lamp, and it is necessary to consider the operating area of the fishing gear with relation to the lamp fishing.抄録全体を表示PDF形式でダウンロード (479K) -
浅野 謙治, 田中 昌一1989 年 55 巻 10 号 p. 1715-1726
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe ovarian maturation and spawning process of Japanese common (chub) mackerel Scomber japonicus was studied on the basis of three years samples. The degree of maturity of ovaries was judged by the external appearance, gonad maturation index and frequency distribution patterns of egg diameters. Frequency distributions of egg diameter of ovaries were classified into 10 profiles. Ovarian maturation begins with enlarging of the egg diameter and increasing of the amount of yolked eggs. When the mean size of the most advanced egg group reaches about 0.6 mm, its increasing seems to stop and only the number of yolked eggs continues to increase. When the mean KG value exceeds 4-5, hydration occurs in a fraction of yolked eggs and these are rapidly released as a batch. It is difficult to find clear relationship between the distribution profiles of egg size and the mean KG of mature ovaries owing to the multispawning trait. The minimum sizes at the first maturity was estimated to be approximately 30 cm.抄録全体を表示PDF形式でダウンロード (715K) -
高橋 豊美, 前田 辰昭1989 年 55 巻 10 号 p. 1727-1739
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーFeeding interrelation between Pleuronectes herzensteini and Pleuronectes yokohamae was studied in Mutsu Bay. Catches in experimental seines and trawls conducted between 1977 and 1986 showed that in general, both species did not differ very much in biomass. Comparisons of the stomach contents indicated that the food segregation between these two species was insignificant during spring and was relatively large during summer. In addition, considering that they exhibit a similar diel and seasonal rhythms of feeding except in winter, an intense potential for competitive interaction of food between these two species was expected, especially in spring. However, from spring to autumn, distinct habitat segregation was found between P. herzensteini and P. yokohamae, and such an ecological segregation reduces their competition for food.
In spring and summer, unlike the P. yokohamae, the CPUE of P. herzensteini were inversely related to the prey biomass, because they were concentrated in West Bay where the prey biomass was less than in East Bay. No particular change from year to year was found in the space utilization pattern of P. herzensteini, despite the fact that the P. yokohamae biomass declined largely after 1983, indicating that the habitat segregation between these two species is basically affected by the physical or chemical conditions of the sea water.抄録全体を表示PDF形式でダウンロード (950K) -
浜野 かおる, 佐藤 昭雄, 柳沢 忠1989 年 55 巻 10 号 p. 1741-1745
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe distribution of thyrotropin-releasing hormone (TRH) in carp brains was studied by a specific radioimmunoassay (RIA). The carp brains were dissected into seven regions. From each region immunoreactive-TRH (IR-TRH) was extracted, purified, and assayed by the RIA. Prior to experiments, the specificity of TRH antisera produced in our laboratory was examined by RIA and enzyme-linked immunosorbent assay (ELISA). These antisera were found to be highly specific. IR-TRH was present in all regions of carp brain. The highest level of IR-TRH was found in the olfactory bulb, and a higher level of IR-TRH was observed in the diencephalon, the pituitary, and the olfactory lobe. These results suggest that TRH has a certain physiological role not only in the endocrine system but also in the central nervous system, and may have a function in olfaction in particular.抄録全体を表示PDF形式でダウンロード (324K) -
山本 勝太郎, 平石 智徳, 小島 隆人1989 年 55 巻 10 号 p. 1747-1751
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーA study was conducted to determine the drag coefficients of float and rope presently used in scallop culture facilities. The drag forces of a polyethylene spherical float (30cm in diameter) and a polypropylene three-strand laid rope (24mm in diameter), with different encrusted conditions of marine organisms, were measured in an experimental flow tank.
The experimental results were drawn in CD-Re diagrams (CD: drag-coefficient, Re: Reynolds number). The average value of CD was 0.75 for the floats encrusted with barnacles, blue mussels and plumed worms in 3.5×104<Re<105, and CD was 0.52 for the float scraped off marine organisms from the surface in 3.5×104<Re<8×104. For the rope when set normal to the flow, CD was 1.4 for the rope with amphipods and skeleton shrimps, and CD was 1.8 for the new rope without organisms in 2×103<Re<104.抄録全体を表示PDF形式でダウンロード (1330K) -
今井 健彦, 中村 毅1989 年 55 巻 10 号 p. 1753-1757
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThis study deals with the fluid dynamical drag coefficient of a plane netting concerning the Reynolds number based on the diameter of a netting twine. Six kinds of Weaver's-knot netting were used for this experiment; the range of the ratio of twine diameter and leg-length (d/l) were from 0.037 to 0.124. The nettings were fixed on a circular shaped frame hung 29.3% of hang-in ratio, and set into a flume tank normal to the flow direction.
The drag was measured by a three component load-cell as a X-component and recorded on a chart-recorder. In this case the drag coefficients were calculated by Newton's law of resis-tance, based on projective area of netting twine.
The current flow was set at 5, 10, 25, 50, 75, 100 and 125cm/s monitored by a super-sonic current meter, and the range of Reynolds number were from 1.7×10 to 1.8×103.
The results obtained were as follows:
1) A Reynolds number of 2×102 was determined to be the critical value for transition of drag coefficient.
2) For Reynolds number greater than 2×102 the drag coefficient was determined to be 1.14.
3) For Reynolds number less than 2×102 the drag coefficient followed the empirical equation: CD(90)=14.81(Re)-0.49.抄録全体を表示PDF形式でダウンロード (321K) -
松宮 義晴, 大西 修平1989 年 55 巻 10 号 p. 1759-1765
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーA more effective releasing program was examined by designing a simulation model which can indicate the survival and growth of released artificial fingerlings. Effects of a carrying capacity on the releasing program were also discussed. Red sea bream in Shijiki Bay was chosen as the subject of the report.
Knowledge on the growth, survival and predation, hypothetical suitabilities of the period (June-August) and site for the releasing and carrying capacity were used as the components by formulating. A biomass of released red sea bream was simulated at intervals of five days until the last day of September, using 243 (=35; three initial values of five variables on the program) initial conditions. Three kinds of the judging criterion were used for evaluating the effectiveness.
When a biomass in the last day of September was used as a judging criterion, the importance of each item of releasing program can be observed in the following order: size of fingerlings, number of released fish, releasing site, releasing period and number of wild fish. Using the increase rate criterion, superior importance of the releasing site can be indicated. In the case of the unit price index of survival criterion, only the releasing period showed extremely little importance and other items showed similar importances. As the effect of carrying capacity increased, the importance of the releasing period increased and that of the number of released fish decreased.抄録全体を表示PDF形式でダウンロード (434K) -
不破 茂1989 年 55 巻 10 号 p. 1767-1771
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe ground rope of the drag nets puts the lower sections of gear components tighly in contact to the sea bed. Considering the fish reactions with the ground rope, the author showed a probability model of fish herding for four types of ground rope, triangle, trapezoid, catenary, and parabola. In this paper the probability that flatfish enter the net was theoretically calculat-ed. Changes of fishing efficiency and relative herding ratio estimated from the probability model were discussed.
Fishing efficiency of four types of ground rope: triangle, trapezoid, catenary and parabol waere compared. The highest fishing efficiency showed when wing tip distance was about 50-60% length of the ground rope in four types of ground rope. The highest fishing efficinecy decreased above 3.0 kt in all types of ground rope.抄録全体を表示PDF形式でダウンロード (292K) -
二宮 学, 村岡 愛一郎, 楠田 理一1989 年 55 巻 10 号 p. 1773-1776
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーTo develop a practical vaccine against pseudotuberculosis, a ribosomal P fraction (RBP) prepared from Pasteurella piscicida was applied to yellowtail Seriola quinqueradiata by immersion method and the immunogenic effects were examined.
Group RBP×1 was immersed once with RBP, whereas group RBP×2 was immersed twice with RBP. Agglutination antibody titer, alternative complement pathway activity (ACH50) and phagocytic index were monitored. Artificial challenge was carried out four weeks after immersion. The agglutination antibody titer against formalin-killed cells did not rise significantly in any groups. ACH50 in the RBP×1 and RBP×2 were lower than that in the control. Phagocytic indexes in all the groups were similar. Percentage survival seven days after artificial challenge were: 11%; control, 0%; RBP×l and 0%; RBP×2. The results suggest that RBP is possibly not effective against pseudotuberculosis in yellowtail by immersion method. Furthermore, the immune response of yellowtail seemed to be suppressed by the immersion vaccination with RBP.抄録全体を表示PDF形式でダウンロード (302K) -
青木 一郎, 稲垣 正, 三谷 勇, 石井 丈夫1989 年 55 巻 10 号 p. 1777-1783
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe applicability of expert systems to predicting fishing conditions of Japanese anchovy Engraulis japonicus off the coast of Kanagawa Prefecture was examined. The prototype system was developed using an expert system “Shell” running on a personal-computer. It was a rulebased and backward chaining production system with a knowledge base consisting of 146 rules. Twenty eight variables in total including egg abundance, catch of larvae, Kuroshio path and so on were considered, and relations between the variables were expressed as rules. The expert system approach was shown to be useful by the test runs that resulted in relatively good reproduction of past catch records when actual data in the past were used.抄録全体を表示PDF形式でダウンロード (458K) -
丸山 功, 安藤 洋太郎, 前田 直彦, 平山 和次1989 年 55 巻 10 号 p. 1785-1790
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe development of the capacity to store vitamin B12 is necessary for improvement in the dietary value of Chlorella as a food for mass production of the rotifer Brachionus plicatilis. In the present study, the uptake of vitamin B12 by fresh-water Chlorella was investigated under controlled culture conditions.
The vitamin B12 content of nine Chlorella strains grown in a medium without enrichment of vitamin B12 were at less than 0.26μg/100g, regardless of the culture methods of autotrophy, mixotrophy and heterotrophy. The test of vitamin B12 uptake from the medium clarified that the strains were divided into two groups. The first group was composed of 5 strains and has the ability to take up more than 81% of the vitamin B12 which had been added to the medium. The other group of 4 strains took up less than 19% of the vitamin B12 supplied. The former group has no ability to produce secondary carotenoids, which are related to the structure and composition of cell walls, while the latter group has the ability.
The addition of vitamin B12 at various concentrations into the culture medium makes it possible for Chlorella vulgaris K-22 to store vitamin B12 in the cells at various levels from 0.2 to 1100μg/100g. The vitamin B12 content of cells remained virtually unchanged during a thirty-day cold storage and during a three-day suspension in artificial sea water.抄録全体を表示PDF形式でダウンロード (442K) -
橋本 伸一, 村岡 愛一郎, 楠田 理一1989 年 55 巻 10 号 p. 1791-1797
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーPseudotuberculosis caused by Pasteurella piscicida causes a grat loss in yellowtail Seriola quinqueradiata. In order to know growth stimulating factors for the pathogen, the effects of 4 carbohydrates, 20 amino acids, 12 vitamins, 16 inorganic salts and 8 peptones, on the growth of P. piscicida strain U-3 isolated from yellowtail were investigated. Each substance, except peptones, was added individually to the basal medium composed of 1 % polypeptone, 0.5% yeast extract and 2% NaCI. Each peptone was added instead of polypeptone in the basal medium. Bacterial growth was compared by measuring optical density at 660 nm during cultivation.
Out of four carbohydrates tested, galactose was the most effective at a concentration of 0.2%. Addition of L-alanine, L-serine, L-asparagine, L-glutamine, sodium L-asparaginate, sodium L-glutamate or L-proline could stimulate growth and among these sodium L-glutamate was the most effective at a concentration of 1.0%. None of the vitamins tested had any stimulative effects. Addition of acetate salts stimulated growth as well. Magnesium acetate was superior than other acetate salts tested and its optimal concentration was 0.5%. Casamino acid was the most effective peptone. Finally, use of casein hydrolysates or soybean meal was capable of growth promoting in comparable beef extract.抄録全体を表示PDF形式でダウンロード (415K) -
于 建平, 日野 明徳, 宇城 正和, 前田 昌調1989 年 55 巻 10 号 p. 1799-1806
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe balance of vitamin B12 in massculture of the rotifer Brachionus plicatilis was calculated for both batch and semicontinuous cultures after the evaluation of B12 concentrations in Nan-nochloropsis, yeast, rotifer and culture water. The B12 output, which is taken as the total of B12 harvested and left remaining in a tank, was 1.6-2.2 times larger than the B12 introduced with food, water and inoculum. On the other hand, among 100 bacterial strains isolated from rotifer tanks, 35 had B12 productivity and 19 of them promoted the growth of the rotifer effectively when supplied with yeast. Calculated from the feeding rate, the B12 content of bacteria and the B12 demand of the rotifer, it is proved that the greater part of B12, the essential vitamin to the rotifer is produced by bacteria existing in water, and excess amount was transferred to the rotifer automatically by grazing.抄録全体を表示PDF形式でダウンロード (548K) -
中山 照雄, 岡 達夫, 大井 淳史, 丹羽 栄二1989 年 55 巻 10 号 p. 1807-1813
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーRestructured sardine meat was prepared from a mixture of washed pulverized sardine meat and sardine oil by adding NaAlg and dialysing against CaCl2 Solution. We tried to shorten the time of dialysis and to prevent quality deterioration in this process. The dynamic rigidity G' and dynamic loss G″ were measured to examine the theological state of the mixture before dialysis. Jelly strength and breaking deformation of the products were measured to observe the progress of the alginate-calcium reaction during the dialysis process. The G' and G″ of the mixture with both sardine oil and water were much smaller than those of the mixture using sardine oil only. The jelly strength and breaking deformation of the products with both sardine oil and water were much larger than those of the products with sardine oil only. It was concluded from the above findings that the smaller the G' and G″ were, the faster the alginate-calcium reaction proceeded. In the sensory evaluation, raw and cooked products with 0.8% NaAlg, sardine oil and water were better than those with 2% NaAlg, sardine oil and water about their texture. The large breaking deformation of both raw and cooked products with 0.8% NaAlg may be related to the good scores.抄録全体を表示PDF形式でダウンロード (1223K) -
矢野 友紀, Remy E. P. Mangindaan, 松山 博子1989 年 55 巻 10 号 p. 1815-1819
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーSchizophyllan, scleroglucan and lentinan, which are β-1, 3-glucans derived from Schizophyllum commune, Sclerotium glucanicum and Lentinus edodes, respectively, were evaluated for their ability to enhance protection to Edwardsiella tarda infection in carp Cyprinus carpio. Intraperitoneal injections of these polysaccharides (2-10mg/kg) to carp 6 and 3 days prior to intraperitoneal challenge with E. tarda (5×107CFU/100g) resulted in a significantly increased survival rate. In all polysaccharide-treated groups, the rapid elimination of the challenge bacteria from the blood was observed and phagocytic activity of pronephros cells against baker's yeast was significantly elevated. Furthermore, it was shown that these polysaccharides activated the alternative complement pathway of carp. These results indicate that schizophyllan, scleroglucan and lentinan enhance the resistance of carp to bacterial infection through the activation of the non-specific immune system.抄録全体を表示PDF形式でダウンロード (356K) -
赤羽 義章, 志水 寛1989 年 55 巻 10 号 p. 1821-1826
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーChanges in the Water Holding Capacity of Surimi Caused by the Addition of Water and Sodium Chloride and by the Subsequent Heat Treatment Yoshiaki Akahane*2 and Yutaka Shimizu*3
Changes in the states of water in frozen surimi of Alaska pollack during grinding with water (25%) and Nacl (2.5%), and the subsequent heating (90°C, 120°C), were investigated. The water in surimi was categorized into three types: Ip, IIp and IIIp, by using our press method. Type Ip water, to be regarded as bulk phase water, increased with adding water into thawed surimi, whereas types IIp plus IIIp water, tightly immobilized in protein network, changed little. However, the type Ip water in ground surimi markedly decreased with the addition of NaCl while types IIp plus IIIp water increased, according to the hydration of the proteins. After the salt-ground surimi was heated, type Ip water increased again and types IIp plus IIIp water decreased proportionally. On the other hand, the type Ip water could be classified into two further sub-states, Ip-A and Ip-B, by compressing gel slices under a series of low pressures between 3 and 10kg/cm2. The type Ip-A water was regarded as “free” and Ip-B as “entrapped” in gels. With the passing of heating time, Ip-A water went on increasing and Ip-B did the reverse, although Ip (Ip-A plus Ip-B) water changed little. The increase of Ip-A water was accompanied by the apparent increment of the released water on the cut surface of specimen. The amount of Ip-A from gels highly correlated with “Hardness” as measured by a texturometer.抄録全体を表示PDF形式でダウンロード (499K) -
赤羽 義章, 志水 寛1989 年 55 巻 10 号 p. 1827-1832
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーWater in Alaska pollack surimi and its gel was categorized into types Ip-A (“free water”), Ip-B (loose “entrapped water”), and IIp (tight “entrapped water”) plus IIIp (“immobilized water”) by our press method. The maximal Ip-A and the minimal Ip-B and IIP plus IIIp in amounts were estimated in both non-salt-ground and salt-ground surimi at a pH of about 5; that is, the water holding capacity (WHC) of surimi was minimal at this pH. The amount of Ip-A in salt-ground surimi with pHs below 5 was larger than in the non-salt-ground one. With the increase of pHs above 5, WHC of surimi markedly increased and the amount of Ip-A decreased more in the salt-ground surimi than in the non-salt-ground material. The amount of Ip-A in surimi at pH7, near the ordinary pH value of surimi for kamaboko, reached a minimum after the addi-tion of 2-3% NaCl.
After heating surimi with pHs between 5 and 9 at 90°C for 40 min, amounts of Ip-A were also less in salt-ground surimi gels than in non-salt-ground ones. Especially small amounts of Ip-A and large amounts of Ip-B were determined in the salt-ground surimi gel at pH 7, near the ordinary pH value of kamaboko with a high WHC. The salt-ground surimi gel with large amount of Ip-A and the low gel-strength was significantly saltier than that with a small amount of Ip-A and the high gel-strength.抄録全体を表示PDF形式でダウンロード (2487K) -
渡部 終五, 潮 秀樹, 岩本 宗昭, Mohamed Kamal, 井岡 久, 橋本 周久1989 年 55 巻 10 号 p. 1833-1839
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe progress of rigor-mortis was followed together with ATP degradation and lactate accumulation during storage at 0 and 10°C of sardine and mackerel spiked at the brain. Both sardine and mackerel showed rapid progress of rigor-mortis, attaining a full-rigor state at a few hours after death. Although rigor-mortis exhibited a large individual variation, its rate was apparently slower with samples stored at 10°C than with those stored at 0°C for both species. ATP and lactate concentrations in sardine muscle were relatively constant a few hours after death irrespective of the storage temperature, in contrast with rigor-mortis progress. Therefore, many sardine samples exhibited a high rate of rigor-mortis even when they maintained a high concentration of ATP, especially with those stored at 0°C. Mackerel gave rise to a more reasonable relationship between rigor-mortis progress and ATP degradation than did sardine. Correlation of ATP content with that of lactate did not markedly differ from each other between samples stored at 0 and 10°C and was calculated to be around r=-0.8 for both sardine and mackerel. It was noted that sardine and mackerel relaxed from a rigor-state at a few hours after spiking when stored at 10°C.抄録全体を表示PDF形式でダウンロード (488K) -
昌子 有, 佐伯 宏樹, 中村 誠, 笹本 泰彦, 野中 道夫1989 年 55 巻 10 号 p. 1841-1844
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーTo investigate the effect of high pressure on fish myofibrillar protein, the inactivation of carp myofibrillar Ca-ATPase in the presence and absence of sorbitol was measured.
The results obtained were as follows: 1) The pressure-induced inactivation of myofibrillar Ca-ATPase accelerated with increasing in pressure and in treating time under the pressure. The myofibrillar Ca-ATPase was inactivated almost completely by a pressure of 2.0 kbar at 0°C for 60min. 2) The mode of pressure-induced inactivation was biphasic first order step; a fast early phase followed by a slow phase. 3) The inactivation of myofibrillar Ca-ATPase induced by the pressure was markedly protected by the addition of sorbitol. The protecting effect of sorbitol depended upon the sorbitol concentration, but not on the myofibrillar protein concentra-tion in the system.
The results suggested that sorbitol suppressed the pressure-induced inactivation of myo-fibrillar Ca-ATPase through the change of water structure in the medium.抄録全体を表示PDF形式でダウンロード (336K) -
Suda Tandavanitj, 奥谷 康一1989 年 55 巻 10 号 p. 1845-1849
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe structure of the extracellular, acidic polysaccharide produced by a marine Pseudomonas No. 32 has been investigated. Isolation and purification of the polysaccharide have been accomplished by precipitation from the culture supernatant with Cetavlon and ethanol, followed by DEAE-cellulose ion-exchange chromatography.
The structure of the purified homogeneous preparation has been investigated by methylation analysis, periodate oxidation, Smith degradation and 1H and 13C NMR spectroscopy. Sulfate was determined by ion-chromatography. It was concluded that the polysaccharide is composed of linear disaccharide repeating-units having the following structure:
→4)-α-L-Rha p-(1→3)-β-D-Man p-(6-O-sulfate) (1→抄録全体を表示PDF形式でダウンロード (1047K) -
森 建太, 尾島 孝男, 西田 清義1989 年 55 巻 10 号 p. 1851-1856
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーCa- and Mg-ATPase activities of akazara striated adductor myosin showed a characteristic feature with a minimum value at about 0.16 M KCl similarly to those of chicken gizzard myosin but not of rabbit skeletal myosin.
On the other hand, all of those myosins showed simlar KCl concentration dependences of filament formation. However, the filaments of the chicken gizzard myosin were dissociated by the addition of μM level of ATP, although akazara and rabbit myosin filaments were dissociated upon addition of mM level of ATP.
Accordingly, the relationship between ATPase activity and filament forming ability is not the same in akazara myosin as that in chicken gizzard myosin.抄録全体を表示PDF形式でダウンロード (2539K) -
尾島 孝男, 西田 清義1989 年 55 巻 10 号 p. 1857-1863
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーThe roles of regulatory light chains in the biochemical function of molluscan myosin were studied by hybridizing “desensitized” akazara scallop myosin with the regulatory light chains of rabbit skeletal, chicken gizzard, rainbow trout dorsal, and crayfish abdominal myosins. By measuring the Mg-ATPase activities of myosin and actomyosin, and the superprecipitation activity of actomyosin, the following results were obtained:
1) All the foreign regulatory light chains we tested, led to the inhibition of Mg-ATPase activities of both desensitized myosin and acto-desensitized myosin in the absence of Ca2+. Whereas, whether the inhibition of Mg-ATPase activities was neutralized by Ca2+ or not, i.e. Ca2+-sensitivity of the Mg-ATPase activities recovered or not, was dependent on the species of the regulatory light chain. In this respect, these regulatory light chains could be classified into two groups; One includes the regulatory light chains of akazara scallop, chicken gizzard, and crayfish abdominal myosins, which afford the Ca2+-sensitivity to desensitized myosin, and the other includes those of rabbit skeletal and rainbow trout dorsal myosins, which afford no Ca2+-sensitivity.
2) The superprecipitation activity of skeletal acto-akazara scallop myosin was remarkably decreased by the removal of regulatory light chains. However, the decreased superprecipitation activity recovered on the binding of either akazara scallop regulatory light chains or foreign regulatory light chains. These results may indicate that foreign regulatory light chains as well as molluscan regulatory light chains can maintain the myosin conformation that is indispensable for actin-myosin interaction.抄録全体を表示PDF形式でダウンロード (573K) -
杉田 治男, 宮島 千尋, 小引 義昭, 出口 吉昭1989 年 55 巻 10 号 p. 1865
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (90K) -
佐伯 宏樹, 若目田 篤, 市原 泰幸, 笹本 泰彦1989 年 55 巻 10 号 p. 1867
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (106K) -
二村 義八朗, 山川 紘1989 年 55 巻 10 号 p. 1869
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (109K) -
朝比奈 潔, 卜部 暁人, 坂井 貴行, 廣瀬 一美, 日比谷 京1989 年 55 巻 10 号 p. 1871
発行日: 1989/10/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (84K)
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