巻号一覧
55 巻, 3 号
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平山 信夫, 山田 作太郎, 菊地 弘, 山田 潤一1989 年 55 巻 3 号 p. 409-416
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーAnalysis using a modified DeLury model to represent daily variation of the catchability coefficient was undertaken. The data utilized were from abalone Haliotis discus hannai fisheryof Kisakata, Akita prefecture in July 1985 and 1986.
A multiplier for the catchability coefficient was expressed in a nonlinear term based on the ratio of operating hours spent for catching abalone to the total fishing hours. The exponent of the nonlinear term, additional parameter to DeLury model, was evaluated by mutliple regression coefficient. Results of the estimates were as follows; abundance is about 3-4t, the catchability coefficient is about 3×10-4h-1, and the daily fishing rate is 3-4%.抄録全体を表示PDF形式でダウンロード (495K) -
中村 義治, 平山 信夫, 秋元 義正1989 年 55 巻 3 号 p. 417-422
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーA method of analysis was developed to estimate biomass and yield of Sakhalin surf clam Spisula sachalinensis through fishing season in dredge fishery.
This model is based on successive growth process of shell size, and composed of three fundamental equations: biomass change, yield, and growth speed of the shell during the fishing season.
Solving the three equations simultaneously, the biomass and CPUE are calculated successively at each time. When applied to actual data of the dredge fishery, the method was found highly valid.抄録全体を表示PDF形式でダウンロード (361K) -
宗原 弘幸, 島崎 健二1989 年 55 巻 3 号 p. 423-429
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーHistological observations were carried out on the ovaries of masked greenling Hexagrammos octogrammus collected in Usujiri, southern Hokkaido, Japan, for the purpose of clarifying the annual reproductive cycle and the first maturational process.
The development of ovaries during maturation was divided into the following six phases;(1) recovery phase (immature phase in case of under-spawners), (2) pre-vitellogenic phase, (3) vitellogenic phase, (4) maturing phase, (5) early spawning phase, (6) late spawning phase.
The vitellogenic phase proceeded for a single month (mid August-mid September), as did the spawning phase (late September-late October).
There were three characteristic features in the mode of oocyte development. The first feature was the transference from the peri-nucleolus stage to the yolk vesicle stage had finished when the most advanced oocytes in an ovary were at the migratory nucleus stage. Second, the maturing through the migration of nucleus to the ovulation progressed intermittently as a clutch. The third feature was oocytes at the oil droplet stage, the early yolk globule stage and the late yolk globule stage began degenerating while the last clutch in this spawning period was at the pre-mature or more developing stage.抄録全体を表示PDF形式でダウンロード (1515K) -
菅野 泰次1989 年 55 巻 3 号 p. 431-439
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーIn order to investigate morphological variations in the ten herring populations in the Far Eastern waters, five meristic and five morphometric characters on body length were examined by employing statistical analysis.
No significant difference was recognized between sexes in all characters except for a BW/BL relation, and significant differences among ten populations were recognized in nine characters except pelvic fin rays. The number of pairs of populations showing significant differences in five characters or more was 31 (69 percent) of all combinations suggesting the diversity of the stocks.
Four groups were distinguished by cluster analysis using Mahalanobis's generalized distances with nine characters and, further, the coefficients of differences on the head length-body length relation showed such large values as to suggest status of subspecies between the groups of the highest distances.抄録全体を表示PDF形式でダウンロード (706K) -
菅野 泰次1989 年 55 巻 3 号 p. 441-445
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーGeographic and environmental causes of variations in meristic and morphometric characters were examined for the ten herring populations in the Far Eastern waters. A study of the correlations between the characters of each population and the latitude, salinity and water temperature at the spawning grounds showed that five characters of initial growth, upper gill rakers, lower gill rakers, head length-body length relation and predorsal length-body length relation are closely related to the latitude or water temperature, but showed no relation with the salinity in the spawning grounds.
Three factors were extracted by factor analysis for the ten morphological characters in populations. One of them which seems to have no correlation with the three environmental properties examined is the factor suggesting genetic differences between the populations. The variations in morphological characteristics may be associated with each population's ecological, adaptitional and quantitative properties during their evolutional courses in the Pacific Ocean.抄録全体を表示PDF形式でダウンロード (434K) -
古丸 明, 和田 克彦1989 年 55 巻 3 号 p. 447-452
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーTriploids were induced in the scallop, Chlamys nobilis, by treating the fertilized eggs with cytochalasin B (0.5mg/l) or hydropressure (200kg/cm2). The highest percentage of triploid was 71.4% in 0.5mg/l CB treatment. When diploids were in spawning period, most triploids had spermatocytes or developing oocytes. However, neither matured oocytes nor spermatozoa were observed in triploids. Although at 9 months of age no quantitative characters differed significantly between diploids and triploids, at 14 months the shell width and meat weight of triploids were greater than those of diploids. The relation between gametogenesis and growth in triploid scallops is discussed in this paper.抄録全体を表示PDF形式でダウンロード (1709K) -
湯本 勲, 絵面 良男, 木村 喬久1989 年 55 巻 3 号 p. 453-462
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーDistribution of the Alteromonas sp., the causative agent of red-spots on the culture bed of makonbu sporophytes in the culture tanks, was estimated at sites of makonbu cultivation in Funka Bay, Hokkaido. This marine bacterium began to appear late in June to early in July when water temperature rose above 13°C, and during the next month appeared in high frequency in this area. But the rate of detection became low when temperature decreased, and the organism could not be detected from winter to spring. It is obvious that the frequency of occurrence of the organism in this area changes during the year.
Attempts were also made to evaluate the route of invasion of this organism into the algal culture tank. The organism was detected from mature fronds and zoospores of makonbu used for cultivation in the nursery. Additionally, the organism, a marine bacterium, requires salts for growth and is labile in heat and dryness. These results suggest that the invasion route of the organism into the algal culture was through mature fronds of makonbu growing in coastal areas contaminated with this organism.抄録全体を表示PDF形式でダウンロード (643K) -
飯田 貴次, 高橋 一孝, 若林 久嗣1989 年 55 巻 3 号 p. 463-465
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe effects of seasonal changes and sexual maturation on the serum bactericidal activity in rainbow trout Salmo gairdneri were investigated.
Two fish populations separated in spawning season were studied. One population reared under natural environmental conditions had spawning period between September and November, while the other reared under controlled day length had spawning period between April and June.
The sera collected from trout were pooled together and mixed with an Escherichia coli suspension. This mixture was incubated at 25°C for 6h. After incubation the number of viable bacteria were counted by the plate counting method and the survival rate was calculated.
Among the heated (45°C for 30 min) sera, the bacteria multiplied about 10 times during the 6h incubation. In contrast, the bacterial count in the normal sera decreased in number to about one tenth of the initial count after 6h. However, the normal sera sampled during spawning period showed an increase of bacteria. This change of the serum bactericidal activity level was observed in both fish populations. In addition, the bactericidal activity level of the sera from fish before being stripped was lower than that from fish after being stripped. From these results, it is probable that the decrease of the serum bactericidal activity during the spawning period increase the susceptibility to disease in fish.抄録全体を表示PDF形式でダウンロード (223K) -
Kasthuri Venkateswaran, 中野 宏幸, 橋本 秀夫1989 年 55 巻 3 号 p. 467-473
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーEcology of the free living forms of bioluminescent bacterial population along with the distribution of total heterotrophs in various sub-water regions of the Seto Inland Sea were attempted. Surface water bolstered the growth of total heterotrophs whereas bottom water endures luminescent procaryotes. Amongst various locale of the sampling area, high population were encountered in the inner regions when collated with the open regions of the Seto Inland Sea. Of 1182 strains identified, 10 different groups of total heterotrophs (867) as well as 8 species of bioluminescent bacteria (315) were observed. Pseudomonas-Alteromonas group was prevalent among TVC and these were decreased from the distance of the coast. Vibrio harveyi was found to be distributed more in all the sampling stations where as 2 species of the genus Photobacterium were noticed less frequently during the present investigation. However, substantiated proportions of V. fischeri was also recorded with some unusual members. Statistical analyses showed that salinity indirectly governs the distribution of these bacterial population and the analyses of variance revealed that these were distributed homogenously and were found to be significant in some sub-water regions.抄録全体を表示PDF形式でダウンロード (497K) -
吉岡 基, 会田 勝美, 羽生 功1989 年 55 巻 3 号 p. 475-478
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーCorrelation between serum progesterone levels and female reproductive status was examined in 46 striped dolphins and 11 short-finned pilot whales taken by the driving fishery. Progesterone levels in immature, resting and lactating individuals were as low as approximately 1ng/ml or below for both species. Pregnant striped dolphins maintained high progesterone levels of 30-40ng/ml throughout the gestation period with fetus over 10cm. The levels of pregnant short-finned pilot whales were also high ranging from 3 to 9ng/ml, but they were lower than those of the pregnant striped dolphins. In striped dolphins, the diameter of corpus luteum of ovulation showed significant positive correlation to serum progesterone levels.抄録全体を表示PDF形式でダウンロード (332K) -
福田 穎穂, 加藤 宜昭, 佐伯 宏樹, 佐野 徳夫1989 年 55 巻 3 号 p. 479-484
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe immunogenic activities of attenuated IHNV clones, AF71-1 and AF71-1-CL3, were examined in this study. These two clones had been purified from successively subcultured IHNV by plaquing, subsequently selected through infectivity trials. Rainbow trout and/or kokanee were immunized with the two IHNV clones by intraperitoneal (IP) injection or water-born method, subsequently challenged with virulent IHNVs. By IP injection, the immunization with AF71-1 was extremely effective in rainbow trout and kokanee, but less or no effect by water-born immunization. The immunogenic activity of AF71-1•CL3, which had been clone purified from AF71-1 by limiting dilution method, was determined in rainbow trout and almost the same results as AF71-1 were obtained. Remarkable protective effect was achieved in AF71-1•CL3 immunized fish by IP injection at the amount of 102.0TCID50/fish.抄録全体を表示PDF形式でダウンロード (477K) -
タナンゴナン・ ジーン・ベラン, 田川 正朋, 田中 克, 平野 哲也1989 年 55 巻 3 号 p. 485-490
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーEffects of thyroid hormones and rearing temperature on Japanese flounder metamorphosis were studied by measuring tissue concentration of thyroxine (T4) by radioimmunoassay. Larvae reared at 15 or 22°C of various developmental stages were quickly frozen and stored at about -32°C until assayed. Non-detectable values of T4 were obtained for pre-metamorphic larvae at both 15 and 22°C. The T4 level increased to a maximum of 16.2ng/g during late metamorphosis, and then decreased slightly. Higher rearing temperature accelerated growth and metamorphosis and also induced earlier increase in tissue T4 levels. Comparison of larvae of similar developmental stages reared in different temperatures revealed no significant difference in T4 levels. These results indicate that thyroid hormones play an important role in Japanese flounder metamorphosis.抄録全体を表示PDF形式でダウンロード (426K) -
吉川 弘正, 上野 三郎, 満田 久輝1989 年 55 巻 3 号 p. 491-498
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe efficacy of cold anesthesia in the transportation of live fish was evaluated using carp acclimated at 23°C. The carp could be safely maintained in an anesthetic state for 5h in water kept at 4°C and in the anesthetized or sedate state for 24h at 8-14°C. Some anesthetized carp showed signs of convulsion when they received external stimuli, and bled mainly from gills. Hemorrhage became distinct with the decrease in temperature and the duration of the cold treatment. The sedate carp showed no such excitement and bleeding. The sedate state is considered to be adequate for transportation, regardless of the anesthetic time; 14°C seems to be the optimal temnerature when the carp are acclimated to 23°C.抄録全体を表示PDF形式でダウンロード (649K) -
河原 栄二郎, 川合 研児, 楠田 理一1989 年 55 巻 3 号 p. 499-501
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーYellowtail Seriola quinqueradiata were infected with Pasteurella piscicida by intramuscular injection, oral infection or immersion exposure. The fate of the pathogen in the tissues was examined by fluorescent antibody technique at selected intervals after infection. After intramuscular injection, the bacteria were located initially in the spleen and kidney then they spread to the gills, pyloric caecum, intestine and heart. After oral infection, the pathogen was found in the stomach immediately after infection then rapidly spread to most of the organs. After immersion exposure, the gill was found to be the most probable site where the pathogen invades. The gill, pyloric caecum, liver, spleen, kidney and heart are the organs in which the bacteria proliferate after invasion.抄録全体を表示PDF形式でダウンロード (232K) -
木村 清志, 岡沢 孝治, 森 浩一郎1989 年 55 巻 3 号 p. 503-506
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe present report deals with the reproductive biology of the tidepool gunnel Pholis nebulosa (Pisces: Pholididae) with particular reference to the morphology of the gonads, seasonal cycle of gonadal maturation, breeding season, size and age at the first sexual maturity, fertility, and sex ratio, based on 355 individuals taken from Ago Bay during the period from April 1980 to May 1982. The testis was bilobed, but the ovary was monolobed in shape. Vitellogenic oocytes in the ovaries and spermatozoa in the testes were present during the period from October to December. Spawning generally began in late November and ended in late December. The minimum sizes at the first maturity for females and males were 15 and 16cm in total length (TL), respectively. The ages of the fish at these sizes were estimated to be age 2 for both sexes. A female spawned only one time in a breeding season. The fertility (F) could be represented by the number of vitellogenic oocytes in the ovary, and it was expressed by the following equation: F=638TL-8672. The sex ratio varied significantly from 1:1 (P<0.01) in favor of females (56.9%).抄録全体を表示PDF形式でダウンロード (1312K) -
加藤 登, 橋本 昭彦, 中川 則和, 新井 健一1989 年 55 巻 3 号 p. 507-513
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーTo prepare the frozen surimi from fish species containing a large part of red muscle as Pacific mackerel and sardine; dilute alkaline (NaHCO3) solution is usually used instead of water for washing of minced meat. For the purpose of further improvement of quality of frozen surimi, we have previously proposed a new procedure by introducing underwater mincing of such fish species.
In order to examine the efficiency of this procedure, the fish muscle in the rigor state with low pH, was filleted and minced in a dilute NaHCO3 solution. The dehydrated minced meat was then mixed with sugar and polyphosphate, and stored at -30°C. During the above treatment, pH of the minced meat was adjusted to around 7.0 and temperature below 10°C.
The improved method could increase Ca-ATPase activity of the frozen surimi by 20% on average and jelly strength of kamaboko prepared from the same surimi by 64% on average, as compared with those obtained by the ordinary method. The jelly strength of kamaboko went up to 610-910g for Pacific mackerel surimi and to 800-1, 260g for sardine surimi.抄録全体を表示PDF形式でダウンロード (608K) -
邱 思魁, 松居 隆, 鴻巣 章二1989 年 55 巻 3 号 p. 515-519
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーAlaska pollack roe and its salted product (“Tarako”) were analyzed extensively for nitrogenous and nonnitrogenous extractive components to elucidate their changes during salting. The change in proximate composition was also examined. The results obtained are summarized as follows.
1) The protein content decreased somewhat, but the lipid content hardly changed during the salting. The content of ash increased remarkably due to salting, and the extractive nitrogen increased to some extent.
2) As for the nitrogenous extractive components in the roe, free amino acids, especially Tau, Ala, Glu, and Leu, were predominant. Considerable amounts of creatine, trigonelline, and trimethylamine oxide were also found. The levels of nucleotides and related compounds were rather low.
3) Among the analyzed nonnitrogenous extractive components in the roe, lactic acid and inorganic ions, such as Na+, K+, Cl-, and PO43-, were dominant. It is noticeable that a relatively large amount of sugar alcohols was detected.
4) The most striking changes in extractive components during the salting were the large decrease in Tau and the large increase in Na+ and Cl-.
5) Free amino acids and inorganic ions were found to constitute the most part of extractives from “Tarako”, suggesting that they were the principal contributors to its flavor.抄録全体を表示PDF形式でダウンロード (323K) -
竹内 俊郎, 渡辺 武, 佐藤 秀一, Ricardo C. Martino, 位田 俊臣, 矢口 正直1989 年 55 巻 3 号 p. 521-527
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーFeeding experiments were conducted with small size of carp in the aquarium to reconfirm the practical levels of protein and energy in carp diets which have been determined with large size of carp raised in the net cages in Lake Kasumigaura. For this purpose juvenile carp weighing 5-7g were fed on experimental diets prepared using practical ingredients and commercial diets for 3-6 weeks.
The feeding results in the aquarium were found to be almost the same as those obtained with large size of carp in the net cages. Namely, it was found that the dietary protein level can be reduced from 39% to 31-35% without reduction of growth and feed efficiency by elevating digestible energy (DE) level above 350kcal/100g diet. On the other hand, some commercial diets low in the amount of available phosphorus resulted in poor growth. The results also indicated that the contents of crude protein, DE and available phosphorus are important factors affecting the growth performance and feed efficiency together with nitrogen excretion in terms of the protein utilization efficiency.抄録全体を表示PDF形式でダウンロード (534K) -
塚正 泰之, 志水 寛1989 年 55 巻 3 号 p. 529-534
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe gel-forming properties of the dorsal muscle from six species of Clupeiformes (sardine, nchovy, pacific herring, round herring, scaled sardine and gizzard shad) and three species of Salmonoidei (rainbow trout, amago salmon and ayu) were compared. The potential gel-forming ability was different among the species. It was corresponding to the protein content in the muscle of those species except anchovy and ayu. By the “setting” (gelation at room temperature) and “modori” (thermal gel degradation at temperature around 60°C) inducing properties of the meat paste, Clupeiformes were divided into three groups; (1) very easily-setting and easilymodori inducing group, sardine, anchovy and pacific herring; (2) easily-setting and very easilymodori inducing group, round herring and scaled sardine; (3) easily-setting and easily-modori inducing group, gizzard shad, and Salmonoidei were divided into two groups; (1) hardly-setting and very easily-modori inducing group, ayu; (2) hardly-setting and hardly-modori inducing group, rainbow trout and amago salmon. Modori phenomena observed for sardine, pacific herring and anchovy, was quite peculiar with respect to that the degradation occurred in a wide range of temperature, between 60°C and 85°C, and did not accompany the breakdown of myosin heavy chain, which is known as an indicator for the typical modori phenomenon.抄録全体を表示PDF形式でダウンロード (503K) -
加藤 尚, 黒倉 寿, 中川 平介, 笠原 正五郎1989 年 55 巻 3 号 p. 535-540
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーEgg lipids of cultured and wild ayu Plecoglossus altivelis were compared. Female fish were obtained from 4 different rivers and 7 hatcheries.
The oil globule mainly consisted of triglycerides (TG), while TG and phospholipids (PL) were dominant in yolk. In the lipid class composition, significant differences could not be found among the samples. Depending on the difference in fatty acid composition in TG of the oil globule and TG and PL in yolk, samples could be divided into two groups. First group was characterized by relatively higher content of C18:1, C18:2 and C22:6. Second group was characterized by higher content of C16:1, C18:3ω3 and C20:5. First group consisted of only the samples obtained from cultured fish and dominant part of the second group was occupied by the samples from wild fish. However, in the samples from cultured fish, there was a sample which could be categorized to the second group.抄録全体を表示PDF形式でダウンロード (492K) -
趙 舜榮, 遠藤 泰志, 藤本 健四郎, 金田 尚志1989 年 55 巻 3 号 p. 541-544
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーTo examine the involvement of enzymes in the oxidation of fish and fishery products, the effect of pre-cooking on oxidative stability of lipids in salted and dried sardine was examined. Lipid oxidation proceeded very rapidly even at 5°C. Particularly in the early stage of autoxidation, phospholipids have been suggested to be the major contributors to the development of lipid oxidation. Oxygen absorbed into salted and dried sardine from the early stage of autoxidation seems to be consumed readily, not only for hydroperoxide formation but also for secondary oxidation product formation. However, lipid oxidation was found to be remarkably retarded by pre-cooking the raw sardine material. Therefore, it was recognized that enzymatic oxidation is an important factor for the oxidative deterioration of salted and dried sardine.抄録全体を表示PDF形式でダウンロード (305K) -
遠藤 泰志, 藤本 健四郎, 趙 舜榮, 金田 尚志1989 年 55 巻 3 号 p. 545-552
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーA model of fish meats was prepared by combining several defatted freeze-dried tissues of sardine with ethyl eicosapentaenoate (EPA). The effects of fish tissues on EPA oxidation were compared quantitatively with those of casein and defatted soybean meal by oxygen uptake, conjugated diene formation, composition of oxidation products and reactions with proteins and other constituents, i.e. browning and fluorescence formation. Furthermore, to observe the involvement of enzymes in the oxidation, raw fish tissue preparations were also incubated with ethyl EPA.
The prooxidant activity of skin, among various sardine tissues was the strongest. The prooxidants in the skin were assumed to be enzymes labile to heat. Hydroperoxide determination (peroxide value) is less reliable as a quantitative measure of oxidation in fish and fishery products because of the instability of hydroperoxides in fish model systems. Thus, the determination of relatively stable secondary products or termination products such as polymers, fluorescent materials and browning matter are thought to be more reliable in evaluating oxidative deterioration in fish and fishery products.抄録全体を表示PDF形式でダウンロード (566K) -
小林 博1989 年 55 巻 3 号 p. 553-557
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーThe effects of the red tide, blooms of Chattonella sp. cultured, on the secretion of gill mucus were investigated in the red sea bream, Pagrus major. Mucous solution was prepared in water extraction from the excised gills. On the day before the experiment, 14C-glucose (5μCi per fish) or14C-glucosamine (0.5μCi per fish) was injected into dorsal muscle of the fish, since radioactivity of the mucous solution was the maximum level on the day following the injection and decreased day by day. In the case of 14C-glucose, radioactivity of the mucous solution extracted from the fish exposed to the red tide was lower than that in the control one, and discharge of the mucus owing to the red tide occurred within 15 min after the exposure. In the case of 14C-glucosamine, no significant difference of the radioactivity was found between the red tide experiment and the control one, although amount of sugar of the mucous solution increased remarkably in the red tide experiment. A possibility is assumed that the mucous solution is composed of several components and the red tide induces some changes in the components.抄録全体を表示PDF形式でダウンロード (401K) -
I. Ketut Suwetja, 堀 貫治, 宮沢 啓輔, 伊藤 啓二1989 年 55 巻 3 号 p. 559-566
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーMarine invertebrates consisting of 9 species of mollusks, 7 of crustaceans and 3 of echinoderms, were analyzed for ATP-related compounds, homarine, and trigonelline during ice storage. The determination was carried out by a newly improved HPLC system which made it possible to separate and micro-quantify the 9 authentic ATP-related compounds. The results showed that besides adenine nucleotides, IMP was detected at the range of 0.1 to 5.5μmol/g in the muscles of all species of mollusks and crustaceans examined, and AdR in only both of the muscle of the oyster Crassostrea gigas and the viscera of the echinoderm Anthocidaris crassispina.
In general, crustaceans accumulated the higher level of IMP than AMP and mollusks did the opposite one during ice-storage. There was no common feature among species of these invertebrates in change in content of IMP during ice-storage.
Two UV-absorbing betaines, homarine and trigonelline could be determined by this HPLC system together with ATP-related compounds. Concentrations of homarine and trigonelline in the muscles of invertebrates examined, ranged from 0.66 to 20.00μmol/g and from 0 to 1.50μmol/g, respectively. There were no apparent changes in content of both betaines during storage in ice up to 10 days.抄録全体を表示PDF形式でダウンロード (501K) -
尾島 孝男, 横本 敬紀, 西田 清義1989 年 55 巻 3 号 p. 567-574
発行日: 1989年
公開日: 2008/02/29
ジャーナル フリーWhen akazara scallop striated adductor myosin reacted with 2, 4, 6-trinitrobenzenesulfonate, one mol of trinitrophenyl(TNP)-group was rapidly incorporated into one mol of myosin. The amount of the TNP-group incorporated in the myosin was not influenced by the presence of 2mM sodium pyrophosphate (PPi), however, the effects of trinitrophenylation on myosin ATPase ac-tivities were remarkably affected by the presence of PPi. Thus, the Ca-ATPase activity was decreased by trinitrophenylation to 80% of its original in the absence of PPi, but to 40% in the presence of PPi. The Mg-ATPase activity both in the presence and absence of Ca2+ was increased by trinitrophen, ?? tion in the absence of PPi, maintaining a relatively high Ca2+-sensitivity. But, in the presence of PPi the activity in the absence of Ca2+ was significantly increased and that in the presence of Ca2+ was slightly decreased, losing its Ca2+-sensitivity.
Both the decreased ATPase activity and Ca2+-sensitivity of TNP-myosin were found to recover by the subsequent dithiothreitol (DTT) -treatment of the myosin. Moreover, the reactivity of the SH1-type thiol of the myosin with N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)-ethylenediamine was found to be remarkably lowered by trinitrophenylation in the presence of PPi but recovered by the DTT-treatment, indicating that trinitrophenylation blocked the SH-group(s) of myosin.
From these results, it is concluded that the triritrophenylation of akazara scallop myosin, especially in the presence of PPi, is accompanied by the modification of the SH-group (s) and that the modification of SH-group causes a decrease not only in myosin ATPase activity but also in Ca2+-regulating ability.抄録全体を表示PDF形式でダウンロード (1502K) -
渡邊 精一1989 年 55 巻 3 号 p. 575
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (78K) -
小林 博, 高橋 幸則, 伊丹 利明1989 年 55 巻 3 号 p. 577
発行日: 1989/03/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (110K)
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