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Volume 55, Issue 8
Displaying 1-27 of 27 articles from this issue
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Hiroo Kitazawa, Yuji Fujikawa, Jiroo Adachi, Nobukazu Tanaka1989 Volume 55 Issue 8 Pages 1297-1304
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDuring the period of 1969 to 1985, annual catch of roundnose flounder increased gradually with some fluctuations till 1978 and then began to decrease in a medium pair trawl fishery of the south-western Japan Sea and its adjacent wasers. This paper examined a recent trend of this stock, using lenght compositions and catch by market size categories in the fishery from 1980 to 1985. The estimated age compositions gave 1.2-1.3 year-1 as estimates of total mortality coefficient in the period of 1980 to 1985. The age compositions also revealed that a recruitment index exponentially decreased at an annual rate of 18% with year at least in the period of 1979 to 1984. Moreover, the exploitation rate for the 1-year-old flounder of 1982 and 1983 year classes decreased to be about one-half of that of 1978-1981 year classes, which were relatively large. Consequently, a decrease of the stock is considered to be caused mainly by a high fishing pressure exerted on the stock, especially on young age classes, in the late 1970's when other important target species continued to decline.View full abstractDownload PDF (504K) -
Tadashi Isshiki, Kenji Kawai, Riichi Kusuda1989 Volume 55 Issue 8 Pages 1305-1310
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe occurrence and levels of yellowtail ascites virus (YAV) and anti-YAV neutralizing antibody were examined in cultured yellowtail Seriola quinqueradiata sampled from one net-pen in Uranouchi-Bay, Kochi Prefecture where an outbreak of viral pancreatic-hepatic necrosis occurred. The sampling period was May 22 to December 2, 1986, the disease was prevalent during May 22 to June 18. The percentage of YAV positive samples in organs of the sampled fish increased after the outbreak of the disease showing 100% during and just after the period of highest mortality, then with decreasing disease prevalence. YAV was not further isolated after 2 weeks from the period of prevalent disease. The mean virus titer (TCID50/g or ml) was 106.6 in the liver, 106.5 in the pyloric caeca, 106.4 in the intestine, 105.7 in the gill, 105.4 in the stomach, 105.3 in the kidney and 106.7 in the ascites fluid. Neutralizing antibody was detected in 10.8% of the fish sampled after July 5. The titers (ND50) ranged from 1:22 to 1:3, 822. No relationship was noted between the level of neutralizing antibody titer and the sampling period or the body weight of the fish.View full abstractDownload PDF (448K) -
Takeshi Hidaka, Kunio Shirakihara, Yasunori Ouchi1989 Volume 55 Issue 8 Pages 1311-1318
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn the Chikuzenkai catch statistics by age and fishery were prepared on red sea bream and stock size was estimated using the cohort analysis in order to get fundamental information on the management.
The ages in the catches ranged from 0 to 6 years old with composition extremely skewed to the younger ages less than 3 which were exploited mainly by one-boat gochi net (surrounding seine) and two-boat gochi net. The older fish were exploited by angling, longline and bottom gillnet.
The number of fish recruited at July of age 0 (three months old) were estimated to be 22-34 millions from 1978 to 1982. The mean survival rates were 24% to age 1, 7% to age 2, 2% to age 3. Reduction of the high fishing pressure to the younger fish was suggested to increase the catches of the older and larger fish.View full abstractDownload PDF (521K) -
Kunio Shirakihara, Takeshi Hidaka, Shinichi Nagahama1989 Volume 55 Issue 8 Pages 1319-1324
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA simple method was developed for analysing the regulation effects in multiple fisheries exploiting one stock competitively, with special reference to the red sea bream stock in the Chikuzenkai. This method is applicable when fisheries are stable from year to year in spite of recruitment fluctuation and when natural mortality rates, individual growth and prices of catches are yearly unchanged.
Two types of fishery production (yield per recruit and fishing revenue per recruit by fishery) were evaluated from an arbitrary cohort, whose values are the same among different cohorts. The sensitivities of such relative production against a reduction in the fishing intensity of a specific fishery were calculated.
Regulation of a particular fishery for the young red sea bream of 0 and 1 year old will benefit other fisheries. Such regulation at specific ages in months will even increase production of the regulated fishery itself.View full abstractDownload PDF (402K) -
Kaworu Nakamura, Imelda Echavarria1989 Volume 55 Issue 8 Pages 1325-1329
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFeeding experiments were undertaken to investigate the acclimation ability of the prawn Penaeus japonicus to different photoperiods. Activity patterns were observed under 24 h photoperiods as 12L12D(12 h, light;12 h, dark), 12D12L, 24dim, 24D and 12dim12D. Forthe individuals of 12D12L and 24D, histological samplings of the brain and optic ganglion were conducted to examine activities of the intercerebral and medulla terminalis cells. The cellular activity was estimated by measuring the relative size of the nucleus (nucleo-cytoplasm ratio).The natural feeding rhythm was disturbed or changed according to the feeding time under respective photoperiods. Achievements of the artificial control of the daytime feeding were shown in the cases of 12D12L, 24dim and 12dim12D, provided that feedings were conducted during morning hours. The cellular activity on the nucleo-cytoplasm ratio indicated that the medulla termnalis cell would continue to show higher values during the daytimc than duringthe night-time regardless of reversal and continuous dark conditions. For the activity of theintercerebral cell, its proper rhythm was disturbed under 24D condition. These results seemedto support the existence of the biological clock in the medulla terminalis.View full abstractDownload PDF (345K) -
Keizou Yabuki1989 Volume 55 Issue 8 Pages 1331-1338
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe present paper treated the age determination of yanagimushigarei Tanakius kitaharai (Pleuronectidae) from otoliths (sagittae). The otoliths used were collected from the 4067 specimens caught off Kyoto Prefecture by a research beam trawl and a Danish seine from 1981 to 1983 and gill nets in 1986. There appear alternating concentric opaque and hyaline zones in an otolith. Each zone was easily read in most otoliths, but acetate replicas of frontal sections were made for a small number of undecipherable otoliths. An opaque zone was formed from spring to summer and corresponded to the duration of a fast growth. Results from age determination based on the number of opaque zones show that the 1980 year-class is a dominant yearclass. Otoliths are recognized to be useful for the age determination of yanagimushigarei.View full abstractDownload PDF (1239K) -
Toshio Yotsui1989 Volume 55 Issue 8 Pages 1339-1342
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTetraspores liberated from the carposporic germlings grew into filamentous thalli. The tissues seperated from the surface layer of the gametophytes, erect Nemalion thalli, regenerated filaments similar to the tetrasporic germlings. When the filaments of tetrasporic germlings and gametophytic tissues were cut into small pieces with razors, they regenerated shoots and grew into new filamentous thalli. By means of cutting treatment, the filamentous thalli propagated in culture. Before cultivation, the filaments were allowed to attach on nori-nets as seedlings according to the following procedures: the filaments propagated in culture were cut into small pieces, and the pieces were scattered on the netting twines immersed in culture vessels fullfiled with culture media, and kept aeration with air compressor for 2 to 3 months. After transfer into the sea in winter, the pieces of the filamentous thalli on the netting twines gave rise to erect thalli in a week, and grew to adult cylindrical Nemalion thalli of 10 to 15cm length after 4 to 5 months.View full abstractDownload PDF (1773K) -
Jun Kohbara, Kazuya Fukuda, Iwao Hidaka1989 Volume 55 Issue 8 Pages 1343-1347
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe feeding-stimulatory effectiveness of a jack mackerel Trachurus japonicus white muscle extract and a synthetic mixture based on a reported analysis of an extract of the jack mackerel white muscle for young yellowtails Seriola quinqueradiata was examined by applying them in starch pellets. Pellets of the natural extract and the synthetic mixture were each offered to the yellowtails separately. The effectiveness of the two stimulants were examined by counting the number of pellets swallowed within the first one min after application of 100 pieces to a group of 20 fish.
The pellets containing the natural extract were completely swallowed, while only 40% of those containing the synthetic mixture were eaten within the test period. The acceptability of both kinds of pellets markedly decreased at one tenth dilution. The effectiveness of the synthetic mixture showed a tendency to increase with increasing concentration, but attained only a 60% of that of the natural extract at a concentration five times that of the original.
The stimulatory effectiveness for the palatine taste receptors of the two kinds of stimulants was also studied by recording the electrical response from the taste nerve. The natural extract was found to be more effective than the synthetic mixture over a wide concentration range tested. The threshold for the two was around -5 and -2 log unit dilution respectively.
From both behavioral and electrophysiological examinations, the above findings suggest that an unknown substance (s) missing in the synthetic mixture may participate in the high stimulatory effectiveness of the natural extract.View full abstractDownload PDF (403K) -
Masaei Kanematsu, Masachika Maeda, Kenzo Yoseda, Hirotaka Yoneda1989 Volume 55 Issue 8 Pages 1349-1352
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIn recent years, fish farmers have begun to culture Nannochloropsis extensively in tanks because it is one of the essential algal foods used to rear especially rotifers. However, algal grazing protozoa frequently occur and reduce the number of Nannochloropsis cells by feeding of them, thus reducing their numbers from 10million cells/ml in a single day. This phenomenon severely disrupts the food rearing process in mariculture.
This protozoa is about 10μm in size, round in shape, has a colorless body, two flagella and a silicated skeletal component. Based on these characteristics, this protozoa was identified as Paraphysomonas sp. It was observed that the growth rate of Paraphysomonas increased with decreasing salt concentration in seawater. The antibiotics, Terramycin was found to repress the growth rate temporary and nickel sulfate was found to reduce a little the activity of this flagellate. It was also observed that when the salt concentration of seawater was increased and physical stress was induced by water disturbance, the growth of the flagellate was repressed as well.View full abstractDownload PDF (2139K) -
Taichiro Fujimura, Tetsuo Kawai, Minoru Shiga, Tadahiko Kajiwara, Akik ...1989 Volume 55 Issue 8 Pages 1353-1359
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSProtoplasts could be isolated from thalli of Ulva pertusa using a mixed enzyme solution of Cellulase Onozuka R-10, Macerozyme R-10, and Driselase. The isolated protoplasts were spherical and 10-40 μm in diameter. The number of protoplasts was 4.8×106 from 1g of fresh weight of the thalli. The purification and fractionation of heterogeneous protoplasts were carried out by a Percoll/mannitol discontinuous density gradient centrifugation, successively, affording a homogeneous population of protoplasts. The percentage of viable protoplasts were more than 90%. The isolated protoplasts developed to the thalli through regeneration of new cell walls, repeating cell divisions, and forming cell colonies. The regenerated plants were obtained on a preparative scale by using an aeration culture of the thalli through an axenic filter in Provasoli's enriched sea water medium (PES) containing antibiotics at 14-18°C under white fluorescent light (14:10h L:D). The present method of the preparative scale-culture might be a large supply of plants for production of valuable substances.View full abstractDownload PDF (2413K) -
Takahito Kojima, Kazumasa Yoza, Hideo Soeda1989 Volume 55 Issue 8 Pages 1361-1370
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAs fish reef block hung in sea water is oscillated by rolling or pitching of derrick barge, theproblem is arising about the safety and accuracy of setting fish reef blocks. In this study, weprepared cubes and fish reef block models and were swung by string from a point which wasoscillated horizontally in still water. By measuring horizontal displacement of model, we calculated velocity and acceleration. Assumed that drag coefficient CD and added mass coefficientCA were not varied with a short period Δt elapsed, we obtained continuous values of CD and CA for each time from the solution of equation of oscillation.
The values of CD and CA during oscillation varied widely with time elapsed and were different from the values obtained in steady flow. Therefore, equation of motion expressed well the oscillation of models by substituting CD and CA obtained from displacement of oscillation into this equation.View full abstractDownload PDF (420K) -
Varin Tanasomwang, Kiyokuni Muroga1989 Volume 55 Issue 8 Pages 1371-1377
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSInvestigations on the aerobic bacterial flora in the intestine of larval and juvenile stages of rockfish Sebastes schlegeli, tiger puffer Takifugu rubripes and red grouper Epinephelus akaara were carried out in relation to the microflora of ambient waters and feeds.
The numbers of bacteria on ZoBell's 2216e agar in the intestine of rockfish (6-18mm in total length), tiger puffer (4-21mm) and red grouper (5-15mm) ranged from 6.0×103 to 3.1×106, 8.6×104 to 2.4×106, and 1.4×103 to 2.6×105 CFU/fish, respectively. A distinct decrease in the bacterial numbers was observed in a group of rockfish when their feeds were changed from live diets (rotifer, brine shrimp and/or copepods) to artificial diet and minced fish. The level of bacterial counts were 104, 107-108, 105, 104 CFU/ml or g respectively in water, live diets, minced fish and artificial diet.
The intestinal microflora of rockfish and tiger puffer was characterized by predominating groups of Vibrio, Acinetobacter and Pseudomonas, while that of red grouper was comprised almost exclusively of Vibrio. However, the incidence of vibrios was much reduced in the group of rockfish which were fed rotifers medicated with sodium nifurstyrenate. The bacterial composition in the intestine of these fishes was found influenced by the microflora of foods ingested rather than ambient water.View full abstractDownload PDF (464K) -
Mikio Oguri1989 Volume 55 Issue 8 Pages 1379-1381
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSHistological investigation was carried out to clarify the distribution of renal juxtaglomerular (JG) cells in the shortnosed sea horse Hippocampus brevirostris known as one of the aglomerular teleosts. The fish were obtained at the Zoological Station of Naples. The kidney tissues from 10 specimens were fixed in Helly's fluid and Bowie's method was applied to tissue stain-ing for light microscopy. In all aglomerular teleosts examined hitherto, the location of JG cells is limited to the inside of the renal parenchyma, though the pattern of distribution shows variation according to species. On the contrary, JG cells in the sea horse were not detected within the renal parenchyma. But they are present as clusters of Bowie-positive cells in the extrarenal connective tissue between the parenchymal portions. This is the first document on the unique distribution of JG cells in the extrarenal region.View full abstractDownload PDF (796K) -
Kenji Hayashi1989 Volume 55 Issue 8 Pages 1383-1387
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe lipid class compositions of the livers of two different size groups and of the mantles of gonatid squid Gonatopsis borealis from the northwest Pacific Ocean were investigated.
In this species, the liver had a remarkably high level of lipids (26.5%), which were characterized by diacyl glyceryl ethers (16.3%) and triglycerides (33.0%). The liver lipids of the small-size squids (mantle length: 13.4 cm) contained relatively higher levels of diacyl glyceryl ethers (27.0%) than those (10.3%) of the large ones (22.5 cm). On the contrary, the mantle contained only trace amounts of these lipids but were high in phospholipids (84.0%).
In the livers of the two different size groups and the mantle of this species, glyceryl ethers originating from diacyl glyceryl ethers were very similar in composition; the most abundant component was chimyl alcohol (38.6 to 45.9% and 66.2%, respectively). Among the tissues examined, differences were found in the percentage of component fatty acids of the total lipids.View full abstractDownload PDF (340K) -
Fukue Shirane, Ichiro Miyazaki, Hiroshi Nozaki1989 Volume 55 Issue 8 Pages 1389-1394
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSHexane extracts from five species of seaweeds in the Inland Sea were analysed, and their content and composition were compared with each other. n-Alkanes, fatty acids and sterols were isolated by silica gel column and thin layer chromatography, and identified by GC, GC-MS, IR and NMR measurements. Composition and amount of n-alkanes and fatty acids are little different between Rhodophyta and Phaeophyta. But, in sterols, cholesterol is dominant in Rhodophyta and fucosterol in Phaeophyta.View full abstractDownload PDF (365K) -
Toshio Takeuchi, Seok-Joong Kang, Takeshi Watanabe1989 Volume 55 Issue 8 Pages 1395-1405
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAtlantic salmon Salmo salar were kept in water with different NaCl concentration (salinity: 0, 1 and 2%) for 48h in order to determine the effects of environmental salinity on lipid classes and their fatty acid composition of gills.
The percentage of gill weight to body weight decreased in fish kept in water with 1 and 2% salinity for 24h. The total lipid of gills (mg/100g fish body weight) also decreased from 897mg of the initial value to 727 and 747mg after adaptation to 1 or 2% salt water for 24h. This was mainly due to decrease of triglyceride class of gill lipids. On the other hand, the content of polar lipids such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was not influenced by salinity levels during 48h adaptation.
The adaptation of salmon to 1 or 2% salt water for 24h remarkably reduced the level of n-3 highly unsaturated fatty acids, especially docosahexaenoic acid (DHA) of PC in gill, while no change was observed in fatty acids of PE. On the other hand, all fatty acids in gill nonpolar lipids were decreased. These results suggest that some selected fatty acids such as DHA in gill polar lipids may have an important role for salinity adaptation.View full abstractDownload PDF (655K) -
Ryuji Ueno, Xiao-Hong Yu, Chong-Sheng Yuan1989 Volume 55 Issue 8 Pages 1407-1413
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTwo acidic β-N-acetylhexosaminidase isoenzymes, form-1 and form-2, were purified to an electrophoretically homogeneous from carp white blood cells, and the enzymatic properties of the two purified enzymes were studied. The two forms, form-1 and form-2, had common pH optimum of 5.0, similar Km values of 0.2mM, and the same molecular weights of 116, 000. They were inhibited to a similar extent by N-acetylgalactosamine and N-acetylglucosamine. They showed both β-N-acetylglucosaminidase and β-N-acetylgalactosaminidase activities. The form-1 and form-2 differed in thermostability and electrophoretic mobility. The form-2 was more heat-liable and had more anodic mobility than the form-1. The isoelectric points of the form-1 and form-2 were 6.2 and 4.8, respectively. The form-2 could be converted to a form that resembles the form-1 by neuraminidase treatment. The properties of the two forms in carp blood were similar with those of hexosaminidases A and B in human tissues.View full abstractDownload PDF (479K) -
Hideki Kishimura, Kenji Hayashi1989 Volume 55 Issue 8 Pages 1415-1420
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA kind of trypsin-like enzyme was purified from crude extracts of pyloric caeca acetone powder of the starfish Asterias amurensis by the following methods; ammonium sulfate precipitation, gel filtration on Sephacryl S-200, and DEAE-cellulose column chromatography.
The SDS-polyacrylamide gel electrophoresis pattern showed that the trypsin-like enzyme was homogeneous. Its molecular weight was estimated to be about 28, 000. The optimum pH and temperature of the trypsin-like enzyme for Tos-Arg-OMe hydrolysis were at around pH 8.0 and 5°C. The trypsin-like enzyme was unstable over 40°C or below pH 5.0, and was neither activated nor stabilized by calcium ions.View full abstractDownload PDF (446K) -
Risako Matsui, Hazime Yamauchi, Hiroshige Suganuma, Masami Ishida, Shi ...1989 Volume 55 Issue 8 Pages 1421-1426
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSIt was previously shown that among fishes in the suborder Salmonoidei, chum salmon and rainbow trout which belong to Salmonidae possessed the skin Type I collagens with a subunit composition of α1α2α3, contrasted to the collagen with (α1)2α2 of ayu which belongs to Plecoglossidae. In this study, soluble skin and/or muscle Type I collagens were isolated from seven fish species in Salmonoidei and characterized with respect to their subunit composition.
Chromatographic and electrophoretic analyses revealed the existence of α1α2α3 heterotrimers in the skin Type I collagens of three other salmonid fishes (Japanese char, masu salmon, and coho salmon) and their α3 chains were distinct in chromatographic behaviour on CM-cellulose from those of many other teleosts. The muscle Type I collagen of Japanese char, however, was composed virtually of an (α1)2α2 heterotrimer, although the possible presence in trace amounts of an α1α2α3 heterotrimer could not be excluded; the same result was previously obtained for the muscle Type I collagen of chum salmon. These composite results indicated the tissue-specificexpression in salmonid fish of a Type I collagen α3 gene. On the other hand, the skin or muscle Type I collagens of several non-salmonid fishes (capelin, Japanese smelt, ayu, and icefish) were found to lack an α3 chain characteristic of many groups of teleosts and existed as (α1)2α2 heterotrimers.View full abstractDownload PDF (2603K) -
Mutsuo Hatano, Koretaro Takahashi, Ayumi Onishi, Yoshiaki Kameyama1989 Volume 55 Issue 8 Pages 1427-1433
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDigital image processing of fall chum salmon was carried out in order to find an objective criterion to analogize the flesh redness from the nuptial coloration.
The external white distribution rate was significantly correlative to the red and yellow distribution rates. And those rates, especially at the ventral to the lateral line portion, showed a negative correlation with the flesh redness represented as “a” value. On the other hand, the external white distribution rate showed a positive correlation with the “a” value of the flesh especially near the lateral line. This tendency was somewhat more specific in female fish than in male fish. And it was considered to be desirable to discriminate the sex of the fish when the flesh redness is ranked from the external characteristics.View full abstractDownload PDF (379K) -
Hisashi Murata, Kiyoshi Yamauchi1989 Volume 55 Issue 8 Pages 1435-1439
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSWe had inadvertently observed an ordinary muscle of cultured red sea bream, Pagrus major, to give an abnormally high 2-thiobarbituric acid (TBA) value. In order to evaluate the extent of lipid peroxidation in cultured fish tissues, we investigated the relationship of ordinary muscle and liver TBA values of 3-year-old cultured red sea breams with their dietary α-tocopherol and lipid levels, including the comparison of the cultured fish and wild red sea breams. The α-tocopherol contents of both tissues from the cultured fish were positively correlated with those of their diets and negatively correlated with the respective tissue TBA values. Thus, a depletion of α-tocopherol from the diets resulted in accelerating lipid peroxidation in the cultured fish tissues with the remarkable effect especially in the case of the ordinary muscle. In addition, the
cultured fish muscle gave an abnormally high TBA value as compared with the wild fish, although their α-tocopherol contents were not significantly different from each other. The results obtained suggested that the cultured fish muscle may be more susceptible to lipid peroxidation than the wild fish and that the dietary α-tocopherol of the cultured fish plays an important role to protect the ordinary muscle against lipid peroxidation.View full abstractDownload PDF (328K) -
Isao Sugahara, Toshio Kimura, Masami Aizawa, Yoshinobu Hayakawa1989 Volume 55 Issue 8 Pages 1441-1447
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSNitrogen-scavenging bacteria were isolated from the waters of Ise Bay, Owase Bay, Kumano Nada, Satsunan Shoto and the surrounding areas during 1983-1986.
Most of the nitrogen scavengers were gram negative, asporogenous motile rods with polar flagella. All isolates were capable of assimilating NH4+ as a sole source of nitrogen. Pseudomonas (52.3%) was the most dominant bacterial genus. Pseudomonas and Vibrio comprised 78.5% of the nitrogen-scavenging bacteria isolated from coastal and oceanic waters of Japan.
The optimum temperature, pH and NaCl concentration for the growth of the representative strains S10, S14 and S174 was near 30°C, pH 7.0-7.5, and 4.0-6.0% (w/v), respectively. Glucose was effective for the growth of nitrogen scavengers. Nitrogen-scavenging bacteria effectively utilized NH4Cl among nitrogenous compounds tested as a source of nitrogen.View full abstractDownload PDF (517K) -
Isao Sugahara, Koichiro Hayashi, Toshio Kimura, Yoshinobu Hayakawa, Ma ...1989 Volume 55 Issue 8 Pages 1449-1456
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSAcetylene-reducing activity of nitrogen-fixing bacterium isolated from coastal region was examined.
The optimum temperature and pH for the acetylene-reducing activity of Vibrio sp. AH 30 was 27-31°C and pH5.0-7.0, respectively. Although Vibrio sp. AH 30 produced nitrogenase in the presence of oxygen, oxygen inhibited considerably acetylene-reducing activity. Low concentrations (0.1-0.2g/l) of polypepton and yeast extract enhanced acetylene reduction by Vibrio sp. AH ?? 30 cells. Sodium pyruvate, mannitol, ATP, NADP(H) and Na2S2O4 also stimulated acetylene-reducing activity of Vibrio sp. AH 30 cells.
The acetylene-reducing activity of nitrogen-fixing bacteria isolated from marine environ-ments was only 1/10 of that of terrestrial N2-fixing ones.View full abstractDownload PDF (510K) -
Kunihiko Konno, Yoh-ich Ueda1989 Volume 55 Issue 8 Pages 1457-1462
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSChanges in several properties of carp myofibrils upon thermal treatment were investigated. Mg-ATPase activity changed in a biphasic manner; increase in an early phase and subsequent decrease. The apparent Mg-ATPase enhancement upon heating was deeply dependent upon ATP and KCl concentration of the assay medium. The first order rate constant of Mg-ATPase enhancement was analyzed by correcting the Mg-ATPase activity using remaining native myosin in myofibrils, and was larger than that of Ca-ATPase inactivation. The extracted protein from myofibrils with 0.5M KCl reduced in an earlier phase of heating, and the decreasing rate was much larger than that of Ca-ATPase inactivation. The large part of the ATPase activity was recovered in the insoluble fraction.View full abstractDownload PDF (418K) -
Kenji Hayashi1989 Volume 55 Issue 8 Pages 1463
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (96K) -
Kaoru Yamanaka, Jun-Gang Wu, Nobuyuki Komagata, Atsushi Suzuki, Ren Ku ...1989 Volume 55 Issue 8 Pages 1465
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (944K) -
Kenji Sato, Reiji Yoshinaka, Mamoru Sato1989 Volume 55 Issue 8 Pages 1467
Published: August 25, 1989
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (98K)
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