NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 55, Issue 9
Displaying 1-28 of 28 articles from this issue
  • Tadanobu Machii, Yukio Nose
    1989 Volume 55 Issue 9 Pages 1493-1497
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The size of Peruvian small purse seines was examined applying the size of anchovy purse seine. Also, a comparison between Peruvian and Japanese purse seines was undertaken. The size adopted was the overall length (Loa; ft) and gross tonnage (GT; t) of the fishing boat, and float line length (Lf; fm), net height (He; fm) and net area (A; fm2) for the fishing net, where the units were fathoms. Analysis was based on the data for 1967, when it was considered that the fishing net in relation to fishing boat had reached a maximum size. The results were expressed for the size of fishing boat as,
    Lf/Loa=3.24, He/Loa=0.484, A/Loa2=1.57, or Lf/GT1/3=49.2, He/GT1/3=7.35, A/GT2/3=362.
    The Lf of Peruvian fishing net was designed shorter than the Japanese one for the same GT.
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  • Hossein Razani, Isao Hanyu, Katsumi Aida, Kiyoshi Furukawa
    1989 Volume 55 Issue 9 Pages 1499-1504
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Under natural conditions, some fish spawn in July-August but the majority spawn in the following March-June. The plasma E2 was at high levels in July and March-April, very low in JanuaryFebruary and rather high in other months.
    When transferred from natural daylength at 24°C to 12L or 16L combined with 24°C on June 22, only a few fish spawned at 24°C/12L with the majority at 24°C/16L in the first 2 months. Then, almost all fish stayed immature for about 11 months. Plasma E2 was low and GtH rather high on both regimes.
    When transferred to 16°C/12L or 16°C/16L, most fish spawned 1-2 times in the first 2 months. At 16°C/16L spawnings were repeated till the 16th month, although intervals were lengthened, while at 16°C/12L spawnings were interrupted for 4-5 months and then resumed. At 16°C/12L, plasma E2 was low in the first six and last 3 months but were high in other months. At 16°C/16L, the E2 fluctuated at rather high levels. The plasma GtH in individual fish in both regimes fluctuated frequently at high levels. At the end, most fish in both regimes were immature and the plasma E2 and GtH were rather low.
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  • Hossein Razani, Isao Hanyu, Katsumi Aida, Kiyoshi Furukawa
    1989 Volume 55 Issue 9 Pages 1505-1510
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Five groups of 2 year old goldfish were cultured at 16°C or 24°C combined with 12L or 16L or natural conditions for 12-17 months starting on June 22.
    Under natural condition, the males were always ready to milt except during August-September and January-March. Plasma testosterone (T) level decreased from late June and reached the lowest in August. The levels were rather high in autumn but lowered again in winter. The T level increased sharply from late March and peaked in April. Plasma gonadotropin (GtH) was low in September and December-February, but high in other months and peaked in August, October, and April.
    At 24°C/12L, most fish milted in September, January-March, and June when plasma T levels were high. In other months, none milted and the T levels were low. Plasma GtH fluctuated in small scales from July till February and in larger scales after March. At 24°C/16L, the males milted heavily in the first month when the T and GtH were at high levels. Thereafter, most fish did not milt, the GtH levels were low, and the T almost undtectable.
    At 16°C combined with 12 or 16L, the males were milting almost continuously. Plasma GtH and T were high and fluctuated several times. Their levels were mostly higher at 12L than at 16L. Key words: Male, Goldfish, Rematuration, Temperature, Photoperiod
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  • Masami Hamaguchi, Takushi Tanaka, Riichi Kusuda
    1989 Volume 55 Issue 9 Pages 1511-1515
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    In order to prepare neutrophils and macrophages, the changes in number and composition of peritoneal exudate cells (PEC) of yellowtail after injection of Pasteurella piscicida formalin killed bacteria (FKB) or proteose-peptone, respectively, were studied. The increases of PEC after injection of P. piscicida FKB was greater than that after injection of proteose-peptone. Most of the cells in the peritoneal cavity of healthy fish were lymphocytes. After injection of any of the two substances, neutrophils, eosinophils and basophils appeared at firstly, and followed by macrophages. The percentage of the lymphocytes gradually decreased. However, the appearance of macrophages in the group injected with P. piscicida FKB was earlier than in the group injected with proteose-peptone. The peak of neutrophils in PEC was observed at 6h after injection of P. piscicida FKB and 12h after injection of proteose-peptone. The peak of macrophages in PEC was observed at 48h after injection of P. piscicida FKB and 192h after injection of proteose-peptone.
    According to the data, P. piscicida FKB shows in elicite the exudation of macrophages into peritoneal cavity than proteose-peptone.
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  • Hiroshi Mayama
    1989 Volume 55 Issue 9 Pages 1517-1522
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The seasonal occurrence of an echinorhynchid acanthocephalan Acanthocephalus sp. in stocked juvenile masu salmon Oncorhynchus masou was examined in the Mena River, southwestern Hok-kaido. The prevalence of infection increased greatly soon after fish were stocked in May but de-creased sharply in summer and early autumn and increased again in late autumn and winter. The mean intensity of infection exhibited more typically seasonal changes than did the prevalence and highly increased in late autumn and winter. Gravid female worms first appeared in June and were the largest proportion between July and September with a peak in August. They decreased in autumn and winter, but immature females increased during these seasons. Isopods Asellus hilge ?? dorfi were infected with Acanthocephalus sp. larvae, and large-sized isopods (⟩4mm) which had higher parasite prevalences than small-sized isopods, were heavily preyed on by juvenile masu salmon during October and January. Thus, the sharp increase in the infection level during late autumn and spring is in accordance with masu's heavy feeding on large-sized isopods in these seasons. The habitat changes of masu salmon from riffles to pools in winter appear to promote the masu's heavy predation on pool-dwelling isopods.
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  • Mitsuhiro Nagata
    1989 Volume 55 Issue 9 Pages 1523-1528
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The satiation time, the satiation amount and the rate of gastric evacuation for juvenile masu salmon Oncorhynchus masou having various sizes were determined at two different water temper-atures (16.2-16.8°C and 8.6-9.4°C). When there was an excess of food, the satiation time was less than 60 min, with a tendency to increase with increasing fish size in both water temperatures. The satiation amount was proportional to W0.715 (W: fish body weight), independent of the water tem-perature. A square root model was more suitable for estimating the gastric evacuation rate than an exponential model. The rate of gastric evacuation for high water temperature was greater than that for low water temperature. From the results of statistical analysis of the difference in the regression parameters between the sized groups, the instantaneous rate of gastric evacuation was constant with respect to the fish size, while the evacuation time was longer in the smaller fish, which consumed a larger meal per unit body weight than in the larger fish.
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  • Jun Ohtomi, Jong-Soo Park, Makoto Shimizu
    1989 Volume 55 Issue 9 Pages 1529-1538
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    From May 1985 to February 1987, sampling surveys with small beam trawlers at 20 stations were carried out every season in Tokyo Bay. With these results and logbook data from several fishermen, the distribution of the Japanese mantis shrimp Oratosquilla oratoria and its relationship to fishing grounds were investigated.
    The distribution of the mantis shrimp covered a wide area of the bay from autumn to winter, but from spring to summer, it was concentrated towards the southern part. From the results of the mark-recapture experiment, this southern concentration is considered to be caused by the movement of the mantis shrimp. The distribution of fishing grounds was concentrated towards the southern part of the bay from summer to autumn and it expands to a wider area from winter to spring.
    In Tokyo Bay, larval mantis shrimp seem to settle in a wide area throughout the bay. As individuals settled in the northern (inner) area of the bay are not fished to any great extent until the following summer, they are secure to be recruited to the stock unless natural death occurs. 1 year-old and over individuals are the target for small beam trawlers and are caught rather intensively in summer and winter.
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  • Mineo Okamoto
    1989 Volume 55 Issue 9 Pages 1539-1546
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Recently underwater observations have begun with small ROVs (Remotely operated vehicle) to observe marine biological resources. The ROV has several advantages compared to diver, because of less limits of observation time and working depth. ROVs are expected to be a good method for biological study around artificial fish reefs. The ability of small observation ROV and divers to distinguish fishes were compared at four characteristic bottom sites. As the result, the ability of ROV was not good enough at the bad visibility areas where young fishes inhabited, but was good at flat bottom areas and relatively good at the artificial reef. In regard to the number of fish species found at the artificial fish reefs settled at a depth of 30m, results of 20 min observations by two divers were superior to those of 360 min ROV observations. When using the ROV for the fishery research in artificial fish reef grounds, the results obtainable are unsuitable to quantitative analysis and extreme care must also be taken not so as to stragle the ROV's cable with fish reefs.
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  • Sakutaro Yamada, Kouji Takagi, Nobuo Hirayama, Kazumi Sakuramoto
    1989 Volume 55 Issue 9 Pages 1547-1552
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    To study the Mazume phenomenon, outdoor tank experiments were conducted taking such factors as the mesh size, the soaking time of the net, the weather and the population density into consideration. The Mazume phenomenon was suggested to exist for higher densities (300 fish) in particular. Higher change of the irradiance per the effective soaking time seems to make fish behavior brisker and the irradiance at which the first catch was observed at dusk was very different from that at which the final catch was observed at dawn.
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  • Chun-woo Lee, Shuzo Igarashi, Takayoshi Mikami, Nariharu Yamashita
    1989 Volume 55 Issue 9 Pages 1553-1558
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The response of pollock to a bait tied to snood and a baited hook were examined in a cylindrical tank using two video cameras from Dec., 1987 to Mar., 1988.
    General features of the hooking process were as follows: pollock swam quickly toward the bait and quickly decelerated in it's immediate vicinity, followed by which a majority of the fish touched the bait, then either sucking it into their mouths or turning away. After sucking the bait into the mouth, hooking took place at the moment the fish made any powerful movement such as jerking or rushing in order to pull the bait off the hook. To be exact, hooking requires a force sufficient to allow the hook point to fully penetrate the inside of the mouth cavity. This force generated at the boundary where a fish's movements are limited by snood length and the velocity difference between the fish's head and the hook in it's mouth. These forces confidence at 95% level ranged from 3.9-5.4 N, with mean value of 4.5 N, as measured by using a steel ring attached to strain gauges placed between the mainline and the snood.
    According to analyses of the video tapes, the time interval between frames was 1/30s, the velocity vector of the fish head at the moment of hooking was described by a jerking move-ment in most cases. However in the case where the bait tied to snood was stolen, the velocity vector consisted mainly of rushing movement.
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  • Oscar Sosa-Nishizaki, Makoto Shimizu, Yukio Nose
    1989 Volume 55 Issue 9 Pages 1559-1564
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Cross-sections of the second dorsal fin rays were used to estimate the age of skipjack tuna Katsuwonus pelamis from the East coast of Japan. For validation of the estimated age, the 19th to 23rd vertebrae centrum were also used to estimate the age. Skipjack tuna were sampled in May, July and September 1985 and from May to November 1986 at Choshi fisheries market (N=230), and from July to October 1986, off Ibaraki (N= 30). The size of the fish ranged from 230 to 624mm in fork length.
    Zero to Five annulus groups were recognized, and 89% of the number of annulus count on cross-sections of the fin rays, agreed with the number of annulus count found on the vertebrae from the same fish. The mean fork length for the fish from the same annulus group are presented and compared with other works. The advantage of using second dorsal fin ray cross sections in the age analysis of skipjack tuna were discussed and are as follows: 1) they are easy to sample, 2) they do not alter the commercial value of the fish, and 3) they can be used as alternative or to validate other aging methods.
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  • Tatsuo Morishita, Kazuaki Uno, Toshiyoshi Araki, Takashi Takahashi
    1989 Volume 55 Issue 9 Pages 1565-1573
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Contents of various nitrogenous compounds in the meats extracts of cultured red sea bream from different localities and those caught in the wild were measured and compared. The cultured fish had a poor content of many free amino acids and trimethylamine oxide, particularly taurine, lysine, alanine and glutamic acid, but were rich in histidine when compared with the wild fish. Fish raised on moist pellets contained more threonine, proline, glycine, histidine, lysine and arginine than fish raised on sardines. Fish pastured in the marifarm contained large amounts of taurine, glycine, alanine, threonine, and proline in comparison with fish cultured in net cages and even with wild fish.
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  • Micho Nonaka, Fumio Hirata, Hiroki Saeki, Yasuhiko Sasamoto
    1989 Volume 55 Issue 9 Pages 1575-1581
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    As a new type of minced fish meat for food stuff, highly nutritional fish meat from sardine minced meat (HNFM) was developed. The manufacturing process is as follows; fresh minced meat is ground in 4 volumes of cold 0.1% NaHCO3 and 0.1% NaCl; removed impurities such as bone and skin; The ground meat is separated from water, lipid and undesirable odor by centrifugation; then it is mixed with additives. HNFM thus obtained contained 60-80% less lipid and about 60% less volatile compounds than the quantities in ordinary sardine surimi, and had only a slight fish flavor.
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  • Hikaru Henmi, Masahiro Hata, Mitsuo Hata
    1989 Volume 55 Issue 9 Pages 1583-1589
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    It was proposed that astaxanthin and/or canthaxanthin in salmon muscle binds with one β-ionone ring to a hydrophobic binding site which exists on the surface of actomyosin. The binding of carotenoids to actomyosin is not specific. The hydroxyl and keto groups of β-end group intensify the binding strength of carotenoids to actomyosin.
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  • Sakae Negishi, Isao Karube
    1989 Volume 55 Issue 9 Pages 1591-1597
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    An enzymatic assay method for IMP determination was developed by using IMP de-hydrogenase purified from Escherichia coli, diaphorase, NAD, thiazolyltetrazolium bromide, and others. An application of this method to the IMP-test paper method was also tried.
    By this colorimetric method, IMP in fish muscle could be determined very accurately. The coefficients of variation and recovery were 0.2-2.5% and 95-108%, respectively; the correlation coefficient between measurements by this method and HPLC method was 0.996 (n=24). As an application, a test paper was devised. A sheet of filter paper was soaked into a solution containing the two kinds of enzyme, NAD, iodonitrotetrazolium chloride, and the inhibitor of lactate dehydrogenase, this procedure was followed by freeze-drying.
    The K-value (a freshness index of fish) was measured rapidly and easily using a muscle homogenate with a combination of this IMP-test paper and an inosine-hypoxanthine test paper previoush developed. The correlation coefficient between the K-value determined by the test paper method and that by the colorimetric method was r=0.993 (n=20).
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  • Michiyo Murata, Morihiko Sakaguchi
    1989 Volume 55 Issue 9 Pages 1599-1603
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The effects of phosphatase treatment of yellowtail Seriola quinqueradiata muscle extracts and the subsequent addition of inosine 5'-monophosphate (IMP) were investigated to elucidate the role of IMP as a flavor substance in fish meat. The treatment of extracts from the fresh white muscle with acid phosphatase resulted in a remarkable decline of flavor intensity (umani, thickness, sourness, fresh fish flavor, and overall taste quality). IMP added back to the enzyme-treated extracts restored the flavor intensity, depending on the concentrations. There was a slight increase in umami intensity at 0.125μmol/ml of IMP and in thickness intensity together with the umami at 0.25μmol/ml. The addition of IMP at 0.5μmol/ml resulted in significant recoveries of all flavor attributes. When 2μmol/ml of IMP was added, the extract showed the maximum flavor intensity; higher levels of IMP did not enhance the intensity any more.
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  • Manabu Muramoto, Nobuo Seki
    1989 Volume 55 Issue 9 Pages 1605-1613
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Carp myosin was fluorescently labeled with 1, 5 IAEDANS at the SH1 thiol group to enable identification of the head portion of the heavy chain where this group is located. The labeled myosin was heated at 35°C for 10min to induce thermal denaturation (95% loss of myosin Ca-ATPase activity) or unheated, and then digested by five different proteases (i. e. calpain, a-chymotrypsin, trypsin, papain, and subtilisin) under the same conditions in 0.05M NaCl at neutral pH, respectively. The proteolytic products were analyzed by SDS-PAGE. The digestion of the unheated native myosin showed protease specific fragmentation patterns. It was, however, found from the denatured and aggregated myosin that all proteases used produced preferentially Mr=150k fragment, corresponding to myosin rod-like fragment due to the absence of the fluorescence, and many other small fragments derived from myosin head portion. The denatured myosin was rapidly digested than native one.
    The cathepsin D from carp skeletal muscle exhibited dual PHoptima at pH3.0 and 4.5 when carp myosin was used as the substrate. At PH3.0, cathepsin D produced mainly Mr=140k and 160k fragments containing the fluorescence on SDS-PAGE from the denatured myosin which was soluble below pH4.0 even though in 0.05M NaCI, while in the denatured and aggregated form above pH4.0 myosin heavy chain was degraded to Mr=150k fragment without the fluorescence.
    It was therefore concluded that the proteolytic cleavage of the denatured and aggregated myosin is remarkably influenced by the specific structural changes of the substrate rather than protease specificity.
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  • Manabu Muramoto, Nobuo Seki
    1989 Volume 55 Issue 9 Pages 1615-1622
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Filamentous carp myosin was incubated at 29°C with or without calpain in the medium containing 0.05M NaCI and 5mm Ca2+ at neutral pH. After the incubation both myosins were solubilized with either SDS, 4M urea or 0.6M NaCl, and then applied on gel filtration chromatography using agarose gel to analyze the mode of existence of the myosins.
    In the presence of SDS, the incubated myosin without calpain as well as native one was fully dissociated into its subunits, heavy and light chains, whereas calpain digested myosin existed as a mixture of Mr=150, 000 (150k) fragment and many other small fragments derived from myosin head portion, S-1.
    In the presence of 4M urea, it was shown that myosin heavy chain and light chains were dissociated from each other but existed independently in their aggregated forms. The 150k fragment also existed in its aggregated form.
    In the presence of 0.6M NaCl, the incubated myosins regardless of, with or without calpain were eluted from the gel column as a single peak at the void volume. It was, therefore, indicated that all myosin fragments, 150k and S-1 fragments, produced by calpain digestion existed in a form of noncovalently associated aggregate rather than a mixture of the fragments in the medium at physiological and higher salt concentrations.
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  • Mutsuo Hatano, Masao Mizogami, Akira Sugawara, Seiichi Ando
    1989 Volume 55 Issue 9 Pages 1623-1627
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The lipid biosynthetic ability in the liver using [1-14C] sodium acetate and [1-14C] oleic acid as precursors and the in vitro hepatic lipase activity were examined to clarify the lipid metabolism of chum salmon. The decrease of triglyceride in the muscle and liver, and the increase of free fatty acid in the liver were found in spawning salmon. The lipase activity increased markedly during spawning migration. The high levels of sterol and triglyceride biosynthetic activities were found in the pre-spawning salmon, whereas phospholipid biosynthesis was almost the same level during spawning migration. The lipid metabolism in the liver was presumed to affect the lipid in the muscle of spawning salmon.
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  • Katsuhiko Harada
    1989 Volume 55 Issue 9 Pages 1629-1634
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The attraction activities for three species of test animals, abalone Haliotis discus, oriental weatherfish Misgurnus anguillicaudatus and yellowtail Seriola quinqueradiata were behaviorally and statistically investigated mainly of L-and antero-dipeptides composed of a neutral amino acid, glycine and one of the basic L-ones, arginine, histidine and lysine at N-terminal. In a comparison test between two kinds of n-and L-dipeptides, and antero-and retro-ones having respectively N-terminal histidine and C-terminal histidine, the activities of L-and retro-types were respectively appreciably higher than those of the D-and antero-types. Among thirty-three specimens of L-dipeptides tested, only glycylalanine was a common attractant for all three test animals, while arginylalanine, alanylhistidine, valyllysine and other 4 peptides were attractants for two species, and
    glycylaspartic acid and other 11 peptides were effective for one species. The attraction activity of dipeptides depended on the species of test animals. In comparison between the activities of dipe-ptides and those of their constituent amino acids, some dipeptides showed apparent synergistic effect in highest degree for both abalone and oriental weatherfish, but the effectiveness of all the attractive peptides for the yellowtail was less than additive.
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  • Takeshi Watanabe, Maria S. Izquierdo, Toshio Takeuchi, Shuichi Satoh, ...
    1989 Volume 55 Issue 9 Pages 1635-1640
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Efficacy of EPA and DHA as the EFA for larval red seabream was evaluated by feeding them rotifers enriched with respectively methyl oleate, EPA, DHA and n-3 HUFA mixture for 8 days in 100l tanks.
    The larvae fed on rotifers containing a low percentage of n-3 HUFA showed low growth and survival rates together with a high incidence of the so-called hydrops. These larvae also showed poor vitality and all of them died in the both vitality tests. The growth and survival rates were effectively improved by the incorporation of EPA and DHA or n-3 HUFA mixture into the rotifers. However, the incidence of hydrops was not completely prevented by EPA and the n-3 HUFA mixture, but was almost totally prevented by DHA. The growth and the survival rate after vitality test were also highest in the larvae fed the DHA-rotifer.
    The fatty acid analysis on the total lipids of larvae has shown that the level of assimilation of DHA was much higher than EPA, although EPA may partly be converted to 22:5n-3 and that no retroconversion of DHA may be available. Thus DHA is found to be superior to EPA as EFA for larval red seabream.
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  • Hideki Ushio, Shugo Watabe, Kanehisa Hashimoto
    1989 Volume 55 Issue 9 Pages 1641-1648
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Fragmented sarcoplasmic reticulum (FSR) was separated from carp ordinary muscle and purified into light (LSR), intermediate, and heavy sarcoplasmic reticulum (HSR) fractions by a sucrose gradient method. Electron microscopy disclosed that most vesicles in FSR fraction were round-shaped, with a few elliptical and asymmetric vesicles. LSR fraction was rich in elliptical and tubule vesicles, while HSR fraction showed many round vesicles with feet structures.
    Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that these fractions had a 97, 000 (97k) Da component as the major. Two ?? 70kDa and one each of 35kDa and 10kDa components exhibited a Ca2+-binding ability in 45Ca autoradiography.
    Ca2+-ATPase and Ca2+ uptake activities of carp FSR were highly dependent on temperature, both being reduced with decreasing reaction temperature.
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  • Akihide Takiguchi
    1989 Volume 55 Issue 9 Pages 1649-1654
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Effects of NaCl on the oxidation and hydrolysis of lipids in salted sardine Sardinops melanostictus fillet, iwashi hirakiboshi, during storage were investigated. Salted sardine fillets with NaCl contents of 0.39, 2.83, 5.36 and 9.19% were prepared and stored at 5°, -3°, -20° and -35°C. The degree of lipid oxidation of the samples was evaluated. When the peroxide value of the extracted lipid and the content of highly unsaturated fatty acid (HUFA) remained unchanged, the extent of lipid hydrolysis was measured by the acid value.
    During salting and drying processes, carotenoid and tocopherol contents in the lipids decreased with increase of NaCl content of the sample. At -3°, -20° and -35°C, lipid oxidation in the samples with NaCl contents of 2.83, 5.36 and 9.19% commenced immediately after storage, while that of 0.39% began 5-30 days after storage, depending upon temperature. The rates of lipid oxidation increased with increase of NaCl content. Lipid hydrolysis occurred during storage at 5°, -3° and -20° and the rates of hydrolysis depended upon the NaCl content and storage temperature: the lower the NaCl content was, the faster hydrosis proceeded. The hydrolysis may be attributed to endogenous enzyme system in sardine meat. In the samples stored at -35°C, little lipid hydrolysis occurred throughout the entire 180-day storage period, regardless of NaCl content of the sample.
    From these findings, it is clear that NaCl accelerates lipid oxidation and inhibits lipid hydrolysis in the salted sardine fillet during storage, though the degrees of accelerative and inhibitory effects of NaCl depend on the NaCl concentration and the storage temperature.
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  • Shigefumi Sasaki, Toru Ota, Toru Takagi
    1989 Volume 55 Issue 9 Pages 1655-1660
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The fatty acid compositions of the lipids in various organs from masu salmon and pink salmon, and in latter canned flesh have been investigated with open-tubular gas-liquid chromatography. All samples showed at least 59 peaks of which 54 components were identified. The major fatty acids of these samples were 14:0, 16:0, 16:1 (n-7), 18:0, 18:1 (n-9), 18:1 (n-7), 20:1 (n-11), 20:1 (n-9), 20:5 (n-3), 22:1 (n-11 and n-13), 22:1 (n-9), 22:5 (n-3) and 22:6 (n-3). The sum of these acids was more than 85% of the total acids in each sample. The major polyunsaturated fatty acids were 20:5 (n-3) (6.34-9.91% in masu salmon, 8.19-16.47% in pink salmon) and 22:6 (n-3) (9.98-18.44% in masu salmon, 4.88-8.56% in pink salmon). The polyunsaturated fatty acids having n-3 structure were contained more in the liver than in other organs. The canned pink salmon flesh showed various fatty acid compositions. Especially, the canned pink salmon flesh C contained 22:1 (n-11 and n-13), ten times as much as the canned pink salmon flesh A.
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  • Joebert D. Toledo, Hisashi Kurokura, Shogoro Kasahara
    1989 Volume 55 Issue 9 Pages 1661
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • Hiroaki Saito, Kunisuke Nakamura
    1989 Volume 55 Issue 9 Pages 1663
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • Morihiko Sakaguchi, Michiyo Murata, Jong-Bae Kim
    1989 Volume 55 Issue 9 Pages 1665
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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  • Takao Matsuno, Eiji Yamashita
    1989 Volume 55 Issue 9 Pages 1667
    Published: September 25, 1989
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
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