The pigment composition of chlorophyll-protein complexes (CPC, zones I-IV) separated by SDS-PAGE from the brown macroalgae
Hizikia fusiformis was determined by using HPLC. The HPLC pigment profiles of chloroplasts showed the presence of chlorophyll a (Chl a), chlorophyllc (Chl c), fucoxanthin, β-carotene, and three minor xanthophylls.
While Chl c was not found in zone I, in CPC, and β-carotene was present in both zone I and zone II, and fucoxanthin, neofucoxanthin, diadinoxanthin and diatoxanthin were found in all of zones I-IV
In the brown orange zone IV, the molar ratio of Chl a/fucoxanthin was the lowest value of all zones, and Chl a, Chl c and fucoxanthin were present in a molar ratio of 3.3:1:2.7.
The yield of chloroplasts reached the maximum value in August, and since then, showed a tndency to decrease according to the growth of
H. fusiformis. In fronds and chloroplasts, the molar ratio of accessory pigments per Chl a became maximum in March.
We demonstrated that zone IV contained over three times as much accessory pigments as other CPC, and that it was a main light-harvesting complex in
H. fusiformis. Furthermore, wo found that molar ratios of xanthophylls and Chl c per Chl a were vaziable throughout the period of growth of
H. fusiformis.
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