NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 56, Issue 9
Displaying 1-27 of 27 articles from this issue
  • Kazuo Fujino, Shigeru Nakajima, Yasushi Ogasawara, Yutaka Yonezawa, Ka ...
    1990 Volume 56 Issue 9 Pages 1371-1381
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Analyses on genotypic compositions in parents and their normal diploid and triploid offspring sib populations have made it possible to estimate rate of gene-centromere recombination at seven loci in the pacific abalone Haliotis discus hannai. Similar analyses on parentage in genotypic compositions in the three mating groups of the Pacific abalone allowed to estimate rate of gene-centromere recombination at alpha-glycerophosphate dehydrogenase, superoxide dismutase, and esterase·M loci. Comparisons between the observed and expected frequencies of genotypes dis-closed a significant excess of homozygotes at Es•M locus commonly in normal diploid and triploid offspring sib populations in some mating group but not in the other. The observation on normal diploid seemed to be consonant with those reported before on juvenile animals sampled from wild population. A series of more careful examination on probable involvement of some influencing mechanism (s) in the occurrence of deviations in genotypic compositions at the locus is now under way.
    Download PDF (865K)
  • Katsumi Tsukamoto, Kazuo Uchida
    1990 Volume 56 Issue 9 Pages 1383-1392
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Interstock differences in spacing pattern and jumping activity were compared for 5 groups (stocks) of juvenile ayu: landlocked lake stock (Wild-L), amphidromous river stock (Wild-R), and reared fish from 3 hatcheries (Reared-A, -B, -C).
    Reared-A and -B formed a tight aggregation (φ15-30cm; diameter) in the daytime, while Wild-L dispersed throughout an experimental tank (φ108cm), and Wild-R exhibited behaviour intermediate between Wild-L and hatchery fishes, forming a loose aggregation and/or schooling in a circle (φ70-80cm). At night, however, there was no difference between the 4 groups; i.e. all fish moved randomly and were inactive. If assuming that a spacing pattern was maintained by an in-terindividual distance (Optimum Distance to the Nearest Neighbour: ODNN) peculiar to a fish group, Wild-L appeared to have a large ODNN, while Reared-A and -B, a small ODNN, and Wild-R, intermediate value.
    Jumping activity was highest in Wild-L (77.9: percentage of the first-time jumpers), followed by Wild-R (60.4), Reared-B (56.3), Reared-C (56.0) and Reared-A (33.6). Diel variations in jumping behaviour of the 5 fish groups were synchronized with each another, showing remarkable peaks from 14:00 till 20:00 hours, corresponding to a rise in temperature of river water.
    In conclusion, spacing and jumping behaviour were closely related to each other: i.e. fish with the larger ODNN in spacing presented the more active jumping behaviour.
    Download PDF (966K)
  • Kazuo Uchida, Katsumi Tsukamoto, Takeshi Kajihara
    1990 Volume 56 Issue 9 Pages 1393-1399
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The jumping behaviour against a waterfall, of ayu is known to be closely related with the tendency to migrate upstream in a river. In order to determine the triggering mechanism for upstream migration in the ayu, the effects of environmental factors on jumping activity were ob-served.
    Jumping behaviour was active in the time corresponding to daytime even under constant light conditions, LL and DD photoperiods, as well as 16L8D. The jumping activity was facilitated by changes in light intensity, a raise in the water temperature, shallow water depth, and deprivation of food. Accordingly, these changes in environmental factors appeared to have the potential to act as a trigger of the migration.
    Download PDF (499K)
  • Kazuo Uchida, Katsumi Tsukamoto, Rikizo Ishida, Shigeo Ishii, Takeshi ...
    1990 Volume 56 Issue 9 Pages 1401-1405
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The spacing pattern and jumping activity were compared for 3 different stocks of juvenile ayu, reared under identical hatchery conditions for about 3 months after fertilization, which were the offspring of landlocked fish from Lake Biwa (LAKE), amphidromous type from the Kiso River (RIVER), and the 14th generation hatchery strain kept at Gunma prefecture (HR-G).
    LAKE dispersed widely in an experimental tank (95cm in diameter) during the daytime, while HR-G formed a tight aggregation and/or schooling with the mean diameter of 48cm. Jumping activity was the highest in LAKE (4.0 times per individual), followed by RIVER (3.3) and HR-G (3.0). These results suggest that parental origin, or genetic factor, controls the spacing and jumping behaviours of the ayu, and thus upstream migration of this species since these behaviours are known to have a close relationship with a tendency to migrate upstream.
    Download PDF (371K)
  • Lucia Fidhiany, Akihiro Kijima, Yoshihisa Fujio
    1990 Volume 56 Issue 9 Pages 1407-1411
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The levels of reproductive isolation between the local populations of the freshwater shrimp Palaemon paucidens in Japan, were estimated on the basis of mating experiments. Postmating isolation has been clearly observed between the A- and B-types which are genetically differentiated based on isozymic analysis. A higher level of premating isolation has been observed between local populations within the A-type, inhabiting ponds and upperstream of rivers. No premating isolation has been observed among different local populations in the B-type, inhabiting downstream or estuarine area. The levels of reproductive isolation are related to the level of genetic differentiation obtained from the different genetic distances.
    Download PDF (336K)
  • Kaworu Nakamura, Keishi Seki
    1990 Volume 56 Issue 9 Pages 1413-1417
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Histological observations were conducted in order to investigate the developmental process of internal organs of the prawn Penaeus japonicus. Organogenesis during metamorphosis was traced from the nauplius to the postlarva stage. Regarding the nervous and digestive systems, the following tissues were differentiated during the six nauplius stages; brain, suboesophageal ganglion, thoracic ganglion, foregut, midgut and hindgut. The midgut gland showed incomplete differenti-ation at nauplius 6. Until this stage, yolk cells remained in the midgut cavity. The midgut gland developed four lobes during the zoea and mysis stages. The midgut cecum was also differentiated at these stages. The results indicated that the larval feeding which begins at the initial zoea stage is not synchronized with the establishment of the digestive gland.
    Download PDF (1099K)
  • Akira Komaru, Hirokazu Matsuda, Takashi Yamakawa, Katsuhiko T. Wada
    1990 Volume 56 Issue 9 Pages 1419-1422
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    For efficient induction of triploid pearl oyster, the effect of cytochalasin B (CB) treatment on meiosis and fertilization of eggs in Japanese pearl oyster Pinctada fucata martensii was investigated. CB treatment (0.1mg/1) was initiated at 5 min after insemination for 10 min (CB 1) or initiated at 15 min after insemination for 15 min (CB 2). In CB 2, two groups of chromosomes at anaphase of second meiosis became two female pronuclei at 33 min after insemination due to the lack of second polar body formation. In CB 2, 51.1% of eggs had two female pronuclei and one male pronucleus at 33 min after insemination. Chromatin of these three pronuclei gradually condensed and became metaphase chromosomes for first cleavage. Eggs with three pronuclei seemed to develop into triploid embryos. In CB 1 the percentage of eggs with three pronuclei was lower (16.6% at 33 min) than that of CB 2. In CB 1 we classified abnormal chromosome behavior of eggs into following typical two types: (1) both first and second polar bodies formation was inhibited. Eggs formed four or five pronuclei. (2) At anaphase of first cleavage one pronucleus derived from second polar body was still observed. The percentage of these abnormalities was higher in CB 1 than in CB 2.
    Download PDF (713K)
  • Patrick Durand, Katsuhiko T. Wada, Akira Komaru
    1990 Volume 56 Issue 9 Pages 1423-1425
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    A combination of caffeine treatment with heat shock treatment on eggs was examined to induce triploidy in the pearl oyster Pinctada fucta martensii. At 31.5°C, different concentrations from 1mM to 13mM of caffeine were applied to eggs before the second meiosis. The increase of caffeine concentration induced an increasing delay or abnormality of metamorphosis from embryo to D-shaped larvae. Histograms of DNA-content in nuclei of cells from D-shaped larvae showed that the highest percentage (35.8%) of triploid D-shaped larvae was induced by 13mM caffeine.
    Download PDF (445K)
  • Ketut Sugama, Nobuhiko Taniguchi, Singo Seki, Hiroshi Nabeshima, Yoshi ...
    1990 Volume 56 Issue 9 Pages 1427-1433
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Gynogenetic diploid red sea bream Pagrus major were produced by fertilizing the eggs with black sea bream Acanthopagrus schlegeli sperm that had been genetically inactivated with ultraviolet (UV) rays, and then 3min. after insemination, heat shocking the eggs at 35°C for 2.5min. to retain the second polar body. This treatment gives a satisfactory survival rate of more than 70% beyond yolk sac resorption. An attempt to produce gynogenetic diploids by suppression of the first cleavage was unsuccessful.
    There are at least 14 fixed differences at enzyme loci between red and black sea bream that can be detected by electrophoresis. No evidence was found of male contribution in two families of gynogenetic diploids, indicatiing a 100% success rate for induced gynogenesis.
    In this study, no differences in the growth rates between control and gynogenetic offsprings was observed up to the age of 6 months. However, significant differences in the growth rate between the two families were found either in control or gynogenetic groups. These differences may be caused by maternal effects as well as genetic factors.
    Download PDF (738K)
  • Wataru Sakamoto, Itaru Uchida, Yasuhiko Naito, Kazuo Kureha, Manabu Tu ...
    1990 Volume 56 Issue 9 Pages 1435-1443
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Loggerhead turtles can maintain a body temperature several degrees warmer than their environ-ment. Stomach temperature data were compiled in order to see how the female loggerhead turtle adapted her body temperature to the different water temperatures near the frontal zone. She passed from the warm water into the cooler water and out again. In the cooler water, the stomach temperature gradually went down. Although this was largely due to thermodynamic transfer of heat from the skin, it was also found that the female loggerhead turtle drinks cold water during a deep dive, thereby cooling body temperature from the inside. Coming out of the cooler water again, she would make continuous and repeated dives in the shallower layer and raise her body temperature once again through kinetic energy consumption.
    Download PDF (616K)
  • Chokei Itosu, Masabumi Seto, Makoto Nakamura, Sadamitu Aketa
    1990 Volume 56 Issue 9 Pages 1445-1448
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The ocean waves have characteristics as a random process. When we design for structures such as breakwaters and harbors, we widely use “the typical wave” as design waves. The typical wave is a single sinusoidal wave and its energy equals to the total energy of the random surface wave.
    The wave force acting on submerged structures is caused by the orbital velocity of water particles, and, therefore, the composed velocity of two waves coming from the different directions has to be added by the vectorial addition.
    However, the typical wave of scalar composition is generally used as design waves. The total energy of the typical wave is different from the energy due to the velocity of vectorial addition.
    In this paper, we theoretically discussed the relation between design wave height on surface structure (H) and that on submerged structure (Hp). we got a formula HP=aH. a is about 0.9 at typhoon.
    Download PDF (222K)
  • Syuiti Kitada, Kohji Shiota
    1990 Volume 56 Issue 9 Pages 1449-1453
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    This paper describes a field study to estimate instantaneous fishing and natural mortality rates of swimming crab from mark recoveries, denoted by F and M, respectively. Efforts are made to eliminate systematic errors which influence estimates. Paint marks on the carapace of mated or egg holding female crab are assured to be retained for a research period from May through July. The observation is censored at 60 days after release. The goodness of fit of the theoretical model to the recovery data is considered and estimates of F and M are obtained. It is concluded that natural mortality of the crab is small and fishing mortality is large in Hiuchi-Nada area.
    Download PDF (337K)
  • Jian-Ping Yu, Akinori Hino, Tamao Noguchi, Hisatsugu Wakabayashi
    1990 Volume 56 Issue 9 Pages 1455-1460
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Vibrio alginolyticus Y5 isolated from a rotifer-culturing pond showed strong fatal toxicity on the rotifer Brachionus plicatilis, and 24h LD50 was around 105CFU/ml. Its injuriousness depend-ing on exotoxin was not affected by the coexistence of the food algae Nannochloropsis oculata. Its propagaion was remarkably promoted when only yeasts, common food for mass culture of the rotifer, or pond water was placed into seawater. Accordingly, it is thought to have a possibility to bring a sudded mass mortality in a rotifer pond. On the contrary, the type culture V. alginolyticus ATCC 17749 did not affect the survival of the rotifer. The toxic factor is thought not to be attribut-ed to TTX, while V. alginolyticus is known as a TTX producer, because 1, 000MU/ml of TTX did not cause mortality of the rotifer, and moreover, Y5 produced netiher TTX nor Anh-TTX.
    Download PDF (392K)
  • Haruyuki Kanehiro, Ko Matuda
    1990 Volume 56 Issue 9 Pages 1461-1466
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    An estimation method for the weight of netting was proposed and weight properties of several knotted and knotless netting were examined.
    Netting weight (W) could be expressed as a function of number of yarns (n) and mesh size (2L) by the following linear relation, W/(n•de)=A+B•(√n•de/p/2L) where A and B are constants respectively expressed as follows; A=23.8q(kD)2 and B=A(b-2a)kD, where q and kD are the degree of packing of netting twine and a constant relating to the twine diameter, and a and b are constants depending on the type of the knot.
    The values of A and B calculated from the netting weight table (W(n, 2L) data) for knotted and knotless netting were found to be constant regardless of the kind of fiber. However, A was different depending on the kind of netting (knot type and twine construction). The value of B was found to be remarkedly different between knotted and knotless netting; B=0.00134 for knotted netting while B=0.00003-0.00009 for knotless netting. It was shown that the weight ratio of both netting changed in the range of 0.7 to 3 depending on the size of 2L and n.
    Download PDF (415K)
  • Rio Yoguchi, Masayo Okuzumi, Tateo Fujii
    1990 Volume 56 Issue 9 Pages 1467-1472
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Seasonal variation in numbers of histamine-forming bacteria was investigated for a one year period in the water samples collected bimonthly from 3 inshore stations in Tokyo Bay and Sagami Bay. Histamine-forming bacteria isolated from these water samples by using histidine broth were classified into 3 types in respect to growth-temperature/salinity: psychrophilic halophitic group, mesophilic halophilic group, and nonhalophilic group. Psychrophilic halo-philic group bacteria were found at the level of 10-102/10l from autumn to the following spring. Mesophilic halophilic group bacteria were detected 10-103/10l from early summer to autumn when water temperatures rose above 20°C. From the taxonomic characteristics, psychrophilic halophilic histamine-forming bacteria were identified with N-group bacteria. But mesophilic halophilic histamine-formers, which can produce as much histamine as Proteus morganii can, could not be identified with any known species.
    Download PDF (444K)
  • Rio Yoguchi, Masayo Okuzumi, Tateo Fujii
    1990 Volume 56 Issue 9 Pages 1473-1479
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Seasonal variation in the number of halophilic histamine-forming bacteria on/in marine fish and changes in their number on marine fish stored at 25°C were studied.
    Psychrophilic halophilic histamine forming bacteria (N-group bacteria) were detected from viscera and skin of fish throughout one year. Besides these mesophilic halophilic histamine-forming bacteria, which were thought to be a new species, were isolated from mackerel during May and July, and from horse mackerel in June, July, September, and November. The occurrence of these bacteria showed similar seasonal variations to that in sea water reported in our previous report.
    During storage of mackerel at 25°C, both types of halophilic histamine-forming bacteria increased in the fish muscle, they could be detected at a cell count of 10-103/g after 16h of storage. Histamine concentration in the muscle after 24h storage increased to become 70-120mg/l00g. This suggested that these new mesophilic halophilic histamine-formers, along with N-group bacteria, were responsible for the scombroid poisoning.
    Download PDF (376K)
  • Mutsuo Hatano, Haruka Iida, Keisuke Seki, Koretaro Takahashi
    1990 Volume 56 Issue 9 Pages 1481-1484
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Occurrence and causes of the unacceptable taste and coloring of Giant Pacific Oyster Crassostrea gigas during frozen storage were investigated.
    Aldehydes, hydroxy fatty acids and free fatty acids interact synergetically so as to cause the unacceptable taste. It was speculated that dimethylsulfide also plays a subordinate part. The water soluble fraction did not exhibit unacceptable taste, while on the other hand, this fraction was responsible for the onset of unacceptable coloring during frozen storage.
    Since unacceptable coloring occurred prior to the development of any unacceptable taste during frozen storage, it was considered that freezing temperature is much more important than glazing in storing the oyster.
    Download PDF (299K)
  • Masato Kinoshita, Haruhiko Toyohara, Yutaka Shimizu
    1990 Volume 56 Issue 9 Pages 1485-1492
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Modori-inducing proteinase (MIP) could be classified into four types as follows on the basis of the extractability from muscle, the optimum temperature for the activity of myosin heavy chain degradation, and the sensitivity to n-butanol. (1) Sarcoplasmic-50°C-MIP (Sp-50-MIP) which is easily extractable, acts optimally at 50°C, and is not sensitive to n-butanol, (2) sarcoplasmic-60°C-MIP (Sp-60-MIP) which is also easily extractable, acts optimally at 60°C, and is sensitive to n-butanol, (3) myofibrillar-50°C-MIP (Mf-50-MIP) which is tightly associated with myofibrils, acts optimally at 50°C, and is not sensitive to n-butanol, and (4) myofibrillar-60°C-MIP (Mf-60-MIP) which is also tightly associated with myofibrils, acts optimally at 60°C, and is sensitive to n-butanol.
    Twelve fish species examined were classified into the following five groups according to the dis-tribution pattern of the above 4 types of MIP. (1) The species having only Sp-60-MIP, such as walleye pollack, mud dab, rainbow trout, brown croaker and red sea bream, (2) the species having both Sp-50-MIP and Sp-60-MIP, such as threadfin bream, (3) the species having both Mf-50-MIP and Mf-60-MIP, such as crucian carp, Pacific mackerel and file fish, (4) the species having both Sp-60-MIP and Mf-60-MIP, such as nibe croaker and tilpia, and (5) the species having Sp-60-MIP, Mf-50-MIP, and Mf-60-MIP, such as shortfin lizard fish.
    Download PDF (1116K)
  • Etsuo Watanabe, Masakazu Hoshi, Masatoshi Takagi, Yayoi Ikeda, Fumio N ...
    1990 Volume 56 Issue 9 Pages 1493-1498
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    GPT or GPT sensor was prepared by a combination of pyruvate oxidase or pyruvate oxidase-oxaloacetate decarboxylase and an oxygen electrode. The optimum condition for each sensor was established, then GPT activity in fish serum was determined by the proposed GPT sensor. GPT activities in fish serum obtained by the proposed sensor system were in good agreement with those determined by a conventional method. One assay could be completed within 20min. Good results were obtained for continuous determination during 10h (30 assays). The conditions for the determination of GPT activities in fish serum were as follows: pH, 7.0; temperature, 33°C; flow rate of sample solution, 0.1ml/min; flow rate of substrate solution, 0.08ml/min; and sample volume, 10μl.
    Download PDF (450K)
  • Yoshiyuki Kumazawa, Ken-ichi Arai
    1990 Volume 56 Issue 9 Pages 1499-1507
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Carp myosin B(2-3mg/ml) was treated at 10°C with 2.OM NaCl or KCl in the presence of various concentrations of sorbitol. During the treatment of myosin B, changes in ATP sensitivity, the amount of denatured actin, and thermal inactivation mode of Ca-ATPase were investigated with a lapse of time. The ATP sensitivity irreversibly decreased and denatured actin increased with the duration of salt treatment. Thermal inactivation mode of Ca-ATPase changed from a single first order step to a biphasic first order process by the salt treatment. The changes induced by NaCl were relatively larger than those by KCl, and sorbitol reduced these changes.
    After the treatment with 2.0M NaCl for 3h, dissociation of myosin from myosin B was recognized by ultracentrifugal sedimentation analysis and gel filtration profile on Sepharose-CL-4B followed by SDS-polyacrylamide gel electrophoretic analysis, and such salt-induced production of myosin monomer was also reduced by the treatment in the presence of sorbitol.
    These results showed that sorbitol suppressed the dissociation of myosin B into actin and myosin induced by high concentration of salt.
    Download PDF (851K)
  • Yoshirou Miyauchi, Shigeru Kimura
    1990 Volume 56 Issue 9 Pages 1509-1514
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Many of teleosts are known to have Type I collagen with subunit structure of α1α2α3. Carp, however, is exceptional in that an α3 chain is absent from the swim bladder Type I collagen, but is present as a minor subunit in the skin Type I collagen. In this study, the skin α3 chain of carp was isolated by CM-cellulose chromatography in combination with Sepharose CL-4B gel filtration and was characterized with respect to its amino acid composi-tion and peptide map. α3 was found to be genetically distinct from αl and α2 and is as-sumed to exist as an αlα2α3 heterotrimer. Moreover, α3 was intermediate in chemical nature between αl and α2, but showed more similarities to αl than to α2. These results suggest that the skin α3 chain diverged from its al chain.
    Download PDF (936K)
  • Misuzu Matsumoto, Hideaki Yamanaka
    1990 Volume 56 Issue 9 Pages 1515-1520
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The changes in contents of glycogen, glycolytic metabolites, and free amino acids (FAA) were investigated in the kuruma prawn muscle during storage at 5°C and 0°C.
    The concentrations of glycogen and glycolytic intermediates were relatively low. During storage, glycogen and glycolytic intermediates decreased and there were no significant accumula-tions in these substances. From these results, it was concluded that glycogen was metabolized smoothly in the kuruma prawn muscle.
    Six amino acids, Tau, Glu, Pro, Gly, Ala, and Arg, accounted for 97% of the total FAA in the kuruma prawn muscle, especially Gly alone 43%. The total FAA decreased during the early storage and showed maximum levels at the initial stage of decomposition regardless of storage temperatures. These changes in the total FAA were mainly due to the contents of the five major amino acids, except for Tau. Orn was not detected immediately after death and increased gradually during storage. Orn appeared to be useful as a potential index for the freshness of the kuruma prawn muscle.
    Download PDF (422K)
  • Keiko Hatae, Misuzu Matsumoto, Atsuko Shimada, Hideaki Yamanaka, Shugo ...
    1990 Volume 56 Issue 9 Pages 1521-1525
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    The dorsal muscle of a carp was sliced into pieces 3mm thick, and six kinds of “arai” were prepared by washing the slices in water of regulated temperature for a specific time: 3 or 5 min in 0°C water, 70 s or 3 min in 18°C water, and 20 s or 70 s in 49°C water.
    The physical property of “arai” was determined by 7 types of measurement. Factor analysis showed that physical properties, of all kinds of “arai” differed significantly from those of untreated fish slices and that “arai” at 0°C and 18°C treatments resembled each other. Treatment at 49°C yielded different results from the others. Sensory panel members could not discriminate the texture of 0°C and 18°C treatment, though they could discriminate 49°C treatment from the others. All of these samples at 0, 18, and 49°C treatment, were judged to be satisfactory.
    After “arai” treatment, ATP content in the fish slices decreased; the higher the tem-perature of treatment, the more the ATP content decreased.
    Scanning electron micrography, showed fewer lipid droplets on the surface of the “arai” slices than on that of untreated fish slices. “Arai” at 49°C treatment was smooth, so we presumed that there was thermal denaturation on the surface. Slight gaps between muscle fibers were found, which were not found in the case of untreated fish slices.
    Download PDF (633K)
  • Toshio Takeuchi, Julius Sampekalo, Tetsuichi Nomura, Takeshi Watanabe
    1990 Volume 56 Issue 9 Pages 1527
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (111K)
  • Tatsuo Hamano
    1990 Volume 56 Issue 9 Pages 1529
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (100K)
  • Michiyo Murata, Morihiko Sakaguchi
    1990 Volume 56 Issue 9 Pages 1531
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (104K)
  • Haruo Sugita, Naoto Okamoto, Yoshiaki Deguchi
    1990 Volume 56 Issue 9 Pages 1533
    Published: September 25, 1990
    Released on J-STAGE: February 29, 2008
    JOURNAL FREE ACCESS
    Download PDF (97K)
feedback
Top