巻号一覧
57 巻, 6 号
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木本 秀明, 檜山 節久, 有江 康章, 小川 浩1991 年 57 巻 6 号 p. 1009-1017
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe status of stone flounder Kareius bicoloratus stock in Sûo-Nada of the Seto Inland Sea, which is considered to be decreasing, was reexamined on the basis of a review and synthesis of the growth curve, fecundity, maturity, etc.
The present status of the flounder is judged as being overfished and the age at culling should be raised from one to about 1.6 years old in order to rebuild the stock.抄録全体を表示PDF形式でダウンロード (672K) -
田中 栄次, 松田 皎, 平山 信夫1991 年 57 巻 6 号 p. 1019-1028
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThis paper proposes a simulation model of the gear efficiencies of trawlers for flatfish in order to predict the effectiveness on the limitations of horsepower of engines and gear scales. Gear efficiency of each boat is defined as (catching efficiency)×(swept area per tow)⁄(size of fishing areas). The catching efficiency is presented by a function of the gear scales, the depth of fishing area and the horsepower of engines, with the probabilities of a fish reacting at contact to ground gears and with distance of jump of fish. The swept area per haul is defined as (distance between two otter boards)×(towing speed)×(towing time), and is expressed by a function of the variables and the towing time. The parameters of the probabilities are estimated by the method of least squares from data of the above variables of boats, CPUE in the same month and fishing area and the distance of jump of fish. From the estimations from the data of five trawlers mean catching efficiencies for bastard halibut Paralichthys olivaceus are 0.031-0.125 haul-1 for (distance of jump)=1-5m. The problems in the model and estimation method, the results of the estimations, and the limitation for applications are discussed.抄録全体を表示PDF形式でダウンロード (654K) -
小林 哲, 松浦 修平1991 年 57 巻 6 号 p. 1029-1034
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe longitudinal distribution and process of migration of the Japanese mitten crab Erio-cheir japonicus were investigated in the Kaminokawa River, Kagoshima Pref. The crabs were collected in crab baskets between September 1985 and August 1986. Crabs were distributed in the freshwater zone throughout most of the year, but were present only from September to January and from March to May in the brackish water zone. Adult females were present mainly from August to December in the freshwater zone, and from September to January in the brackish water zone. Puberty molting by females was considered to occur mainly in August. Active migration of crabs large enough to be caught with crab baskets was considered to occur mainly from September to December. Nearly all the year round, larger crabs were distributed in the upper reaches. In the case of females, the minimum size of adults and the maximum size of juveniles gradually increased further upstream. Therefore, it is presumed that crabs migrate upstream as they grow older. Crabs arriving at the upper reaches will grow larger or older during the juvenile stage. Consequently, juveniles of larger size in the upper reaches become larger adults.抄録全体を表示PDF形式でダウンロード (647K) -
中嶋 正道, 木島 明博, 藤尾 芳久1991 年 57 巻 6 号 p. 1035-1041
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーRelationship between fitness advantage of isozymic genotype and a state of genetic constitution in a stock was examined in cultured stocks of rainbow trout in Japan. A number of stocks exhibited a larger average standard length in heterozygouse individuals under a state of “heterozygote excess” stocks at the Idh-3, Pgm-2 and Sod, significantly. However, an additive effect of advantage of heterozygotes at the three loci could not be observed. It sug-gests a fitness advantage of individuals heterozygous at each locus in cultured stocks of rainbow trout. On the other hand, a few stocks exhibited a larger standard length in homo-zygous individuals under a state of “homozygote excess” at the three loci, suggesting a fitness advantage of homozygous individuals. The mechanism of such phenomena could be explained by the relationship between the state of genetic constitution and quantity of dereterious genes in such a stack. An appearance of a larger average standard length in heterozygotes than in homozygotes could be explained by the resulting of a low fitness in homozygote individuals including many deleterious genes in the stocks. An appearance of larger average standard length in homozygotes than in heterozygotes could be explained by the elimination of deleterious genes during the process of inbreeding抄録全体を表示PDF形式でダウンロード (492K) -
荒井 克俊, 藤野 和男, 清 直人, 千葉 毅, 河村 宗郎1991 年 57 巻 6 号 p. 1043-1055
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe present paper describes the successful induction of gynogenetic diploid and triploid animals in the brook trout Salvelinus fontinalis and the hybrid between the brook trout and the Japanese charr S. leucomaenis by application of hydrostatic pressure shock to eggs after fertilization with genetically inert sperm or normal sperm. Analyses on isozyme genotypic compositions in the parents and their gynogentetic and/or triploid offsprings made it possible to estimate the rates of gene-centromere recombination (x, 0≤x≤0.5) at eleven loci, indicating 0.341 at Alcohol dehydrogenase, 0.457 at α-Glycerophosphate dehydrogenase-A, 0.398 at Isocitrate dehydrogenase-C, 0.049 at Lactate dehydrogenase-A1, 0.023 at Lactate dehydrogenase-A2, 0.011 at Lactate dehydrogenase-B2, 0.439 at Malate dehydrogenase-C/D, 0.465 at Malic enzyme-A, 0.443 at Phosphoglucose isomerase-A, 0.045 at Phosphoglucomutase-B and 0.489 at Superoxide dismutase. Map distance thus obtained in the present work revealed the value close to that reported in the other species of the genus Salvelinus and Salmo at each locus of probable homology.抄録全体を表示PDF形式でダウンロード (1740K) -
河原 栄二郎, 稲荷森 輝明, 野村 節三, 高橋 幸則1991 年 57 巻 6 号 p. 1057-1063
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーWhite-spotted char Salvelinus leucomaenis were immunized intramuscularly with Aeromonas salmonicida extracellular products (ECP). By immunization two or three times at four week intervals, the serum agglutinating antibody titers at twelve weeks after the first immunization were raised ranging from 1:128 to 1:512 or from 1:512 to 1:4, 096 respectively. Disc electrophoretic analysis did not show any difference in the protein composition between im-munized and non-immunized fish sera. By immunodiffusion analysis, char anti-ECP sera formed a single precipitating line against ECP and salmolysin. The component protein of the char antisera, which reacted to ECP, also formed a single precipitating line against ECP in β to γ-globulin region by immunoelectrophoresis. The antiserum was separated into three frac-tions by gel filtration on Sepharose 6B, and antibody activity was found in early eluate of the second peak with Kav of 0.4 to 0.6. The results indicate that salmolysin, which is included in ECP, possesses high immunogenicity to white-spotted char.抄録全体を表示PDF形式でダウンロード (1924K) -
堀木 信男1991 年 57 巻 6 号 p. 1065-1070
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーAnnual changes in the catch of marine juvenile ayu were examined with special reference to the cause of the rapidly decreasing catch in recent years.
The influence of various factors such as environmental changes on ayu resources used to be small in the days when good and poor catches of ayu were repeated naturally. Even when an influence was found, it was corrected in a short period. It seems that the abnormal shortage of water in the autumn of 1984 caused a negative effect on the reproduction of ayu and such a situation subsequently resulted in the rapid decrease in the catch of marine juvenile ayu in the spring of the following year. In addition, a positive correlation was found between the amount of precipitation during spawning season and the catch of marine juvenile ayu in the following spring.抄録全体を表示PDF形式でダウンロード (397K) -
吉越 一馬, Yoshio Kô1991 年 57 巻 6 号 p. 1071-1078
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe ultrastructure of midgut cells of six species of parasitic copepods was observed. The anterior zone of the midgut of Conchyliurus quintus, Ostrincola koe, Panaietis yamagutii, Neo-ergasilus japonicus and Lernaea cyprinacea consisted of vacuolar cells and non-vacuolar cells. The vacuolar cells were characterized by sizable endocytotic and secretory vacuoles suggesting active absorptive and secretory functions, respectively. The secretory product containing digestive enzymes was discharged by holocrine secretion. The non-vacuolar cells were char-acterized by the well-developed microvilli suggesting an absorptive function of the cells. In Pseudomyicola spinosus, however, the anterior zone of the midgut consisted of non-vacuolar cells only containing numerous primary lysosome-like vacuoles. Such lysosome-like vacuoles situated just underneath the free surface and the well-developed microvilli of the non-vacuolar cells suggested their secretory and absorptive functions. Numerous bacteria occurred con-sistently in the anterior zone of the midgut of Ps. spinosus.抄録全体を表示PDF形式でダウンロード (4184K) -
板倉 茂, 今井 一郎, 伊藤 克彦1991 年 57 巻 6 号 p. 1079-1088
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーSpatial distribution of cysts of the noxious red tide flagellates Chattonella (Raphidophyceae) in bottom sediments were investigated in the Eastern Seto Inland Sea (Harima-Nada, Osaka-Bay and Kii-Suido, 70-72 stations), in both May 1988 and May 1989. Densities of the cysts in sediments (0-3cm in depth) were enumerated by the direct count method (for total living cysts) and the extinction dilution method (for germinable cysts). The cysts were widely detected in the whole area of the Eastern Seto Inland Sea (0-1, 503 total living cysts/cm3•wet sediment). In these two years, high concentration areas of Chattonella cysts were noticed in northern to eastern middle parts of Harima-Nada, the eastern part of Osaka-Bay, and middle to western parts of Kii-Suido. Local hydrographic and sedimentary conditions presumably account for the condensed deposition of the cysts in these areas. Mean densities of total living cysts between the two years showed little difference in Harima-Nada (201 and 238/cm3) and Osaka-Bay (62 and 79/cm3), whereas the total living cysts decreased about 1/2 in Kii-Suido (from 425 to 198/cm3). Mean densities of germinable cysts decreased <1/2 in Harima-Nada (from 100 to 45/cm3), Osaka-Bay (from 30 to 13/cm3), and Kii-Suido (from 107 to 25/cm3). The percentage of germinable cysts to total living cysts also decreased in each area (Harima-Nada: from 50% to 19%, Osaka-Bay: from 49% to 16%, Kii-Suido: from 25% to 13%) between the two years. The correlation between total living cyst abundance and germinability of the cysts was not significant in either year. The red tide of Chattonella was observed in August 1987, whereas it did not occur in the summer of 1988. The results of this study suggest that the abundance of vegetative cells in the preceding year affects the proportion of germinable cysts to total living cysts in the following summer.抄録全体を表示PDF形式でダウンロード (725K) -
小倉 未基, 石田 行正, 伊藤 外夫1991 年 57 巻 6 号 p. 1089-1093
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーGrowth variation of coho salmon in the western North Pacific Ocean from 1973 to 1987 was examined on the basis of fork lengths and scale measurements. Average fork lengths of coho salmon sampled by gillnets in July in the area of 44-50°N latitude and 160-180°E longitude showed long-term variations, decreasing from 1975 and increasing from 1983, and also showed strong odd-even year fluctuation. The scale measurements indicated that long-term variations in growth occurred during the freshwater period and during the first year of ocean life. Substantial odd-even year fluctuation in growth occurred only during the second year ocean life. We hypothesize that intraspecific density-dependent growth is an important factor affecting growth during the freshwater stage and interspecific density-dependent growth is important during the second year ocean life, the latter due primarily to the overlap of diet between coho and pink salmon.抄録全体を表示PDF形式でダウンロード (504K) -
上田 泰司, 石井 紀明, 中原 元和, 中村 良一, 鈴木 譲, 清水 千秋1991 年 57 巻 6 号 p. 1095-1102
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーAs one of serial studies on the variation of elemental concentrations in marine organisms, major elements (Ca, K, Mg, Na, P) and trace elements (Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Sr and Zn) in 263 specimens of gonad of the sea urchin Strongylocentrotus nudus were determined by inductively coupled plasma atomic emission spectrometry.
The differences of the elemental concentrations in gonad were not significant among three sampling years of 1984, 1985 and 1986, although annual differences of gonad indexes were observed. Seasonal variation in the elemental concentrations was observed on magnesium, sodium, phosphorus and zinc. Furthermore, magnesium, phosphorus and zinc indicated clear differences by sex. For example, the concentration of zinc in ovary was several times higher than that in testis. On the contrary, the concentrations of magnesium and phosphorus in testis were higher than those in ovary.抄録全体を表示PDF形式でダウンロード (342K) -
岸田 達1991 年 57 巻 6 号 p. 1103-1109
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe abundance of larval and juvenile Japanese Spanish mackerel Scomberomorus niphonius in Hiuchi-nada, the central part of the Seto Inland Sea, and its adjacent waters, was investi-gated by net sampling from 1983 to 1988. The relationships between larval/juvenile abundance, CPUE of subsequent 1-year-old fish and oceanographic conditions were examined. There was a significant, positive correlation between the abundance of juveniles and the CPUE of subsequent 1-year-old fish, although no such correlation occurred between postlarval abundance and either the abundance of juveniles or the CPUE of subsequent 1-year-old fish. This indicates that significant mortality occurs throughout the larval stages and that year-class strength seems essentially determined by the juvenile stage. A strong year-class seems to occur when the boundary between stratified Hiuchi-nada, the spawning area, and vertically well mixed adjacent regions becomes obscure in June抄録全体を表示PDF形式でダウンロード (551K) -
上田 宏, 広井 修, 山内 晧平, 原 彰彦, 香川 浩彦, 足立 伸次, 長浜 嘉孝1991 年 57 巻 6 号 p. 1111-1114
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーFollowing the previous study, serum steroid hormone profiles and in vitro steroid hormone production in the testes of male chum salmon Oncorhynchus keta were examined by adding materials sampled at the North Pacific Ocean. The maximum serum level of 11-ketotestos-terone (11-keto T; 56ng/ml at the coastal sea) preceded that of testosterone (T; 48ng/ml at the pre-spawning period). Levels of both androgens in serum decreased in the spawning period when serum 17α, 20β-dihydroxy-4-pregnen-3-one (17α, 20β-diOHprog) level rapidly in-creased. Incubation of testicular fragments from testes of the coastal sea and the pre-spawning period with 17α-hydroxyprogesterone (17α-OHprog; 0.1μg/ml), T (0.1μg/ml) and chum salmon gonadotropin (SGA; 1μg/ml) resulted in elevated 11-keto T concentration in the incubation medium. 17α, 20β-DiOHprog level in medium was highly increased only in the presence of 17α-OHprog. The conversion of 17α-OHprog to 17α, 20β-diOHprog was enhanced by adding SGA to the incubation medium of testicular fragments of individuals collected from the river (pre-spawning period), but not from the coastal sea. These results indicate that 11-lceto T and 17α, 20β-diOHprog respectively are the major steroids responsible for latter phase of sperma-togenesis and spermiation in male chum salmon.抄録全体を表示PDF形式でダウンロード (294K) -
梅津 武司, 村井 衛, 三木 誠, 倉田 洋二1991 年 57 巻 6 号 p. 1115-1120
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーBy neutron activation analysis (NAA), the Ir content of scutes and bone was determined in hatchlings over a 1, 550 day period, following feeding with 100-200mg Ir per capita with a 2000 ppm diet for 14 days. In the specimen of No. 198 Ir ranged from 0.48 to 167 ppb in 46 bone fragments on day 1, 110, in which the highest Ir occurred in the portion with the slowest growth (target), located in the middle of peripheral bone, under the scute junction, near the margin on the ventral side. After 30 years Ir remains at about a 0.7 ppb level, this determined by the regression line between Ir content and time, calculated from 90 results. To discriminate the Ir mark depends on the Ir content in bone of natural turtles (=0.01 ppb), and the detection limit is estimated to be 0.1 ppb. The Ir mark is therefore very useful up to 30 years or more, however, Eu is not useful in turtles.抄録全体を表示PDF形式でダウンロード (378K) -
村本 光二, 神谷 久男1991 年 57 巻 6 号 p. 1121-1126
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーA method was developed for analysis of sugar compositions of glycoproteins electroblotted onto polyvinylidene difluoride (PVDF) membranes after SDS-polyacrylamide gel electrophoresis. It was based on hydrolysis of glycoproteins on PVDF membranes and precolumn derivatization of liberated neutral sugars, sialic acids and aminosugars, respectively, and isocratic reversed-phase high-performance liquid chromatographies of these derivatives. The presence of sialo-glycoproteins in echinoderm shell matrixes was demonstrated by this method.抄録全体を表示PDF形式でダウンロード (1098K) -
村田 裕子, 林 哲仁, 渡辺 悦生, 外山 健三1991 年 57 巻 6 号 p. 1127-1132
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーFrom the point of view of utilization of fish processing waste, the manufacturing method of skipjack spermary extract by autolysis was previously reported.
In this experiment, a higher nitrogen extract than that prepared by autolysis was obtained from skipjack spermary by enzymolysis.
Each of the enzymes which was prepared from the liver or pyloric caecum of skipjack and purchased from a local store was incubated with minced raw spermary and digested at 37-50°C and pH 2.7-8.0 for 4h. Comparison of total extractive nitrogen and free amino acid composi-tion in the supernatant of the materials and sensory evaluation by the panel revealed that enzyme reaction at 37°C and pH 6.4 for 4h with the crude enzymes from the liver was the most convenient for extract production. This manufacturing method was more efficient in the recovery of nitrogenous extractive compounds than autolysis, as already reported. Three commercial enzymes seemed to contribute to the formation of favorable taste in the extract.抄録全体を表示PDF形式でダウンロード (928K) -
土谷 英樹, 関 伸夫1991 年 57 巻 6 号 p. 1133-1139
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーWhen purified α-actinin from carp ordinary muscle was treated with calpain type II from the same muscle, SDS-PAGE revealed that α-actinin subunits were cloven into fragments of slightly lower molecular mass. On Sephacryl S-300 gel filtration, however, calpain-treated α-actinin was eluted at the same position as that of the native one, suggesting that the dimeric subunit composition still remained after calpain digestion under the physiological condition. The binding ability of α-actinin to F-actin at low temperature was slightly increased by calpain treatment.
It was also found that calpain clove not only isolated α-actinin but also α-actinin within carp myofibrils. In addition calpain showed an another function to solubilize and release both native and digested α-actinins from the myofibrils.
These results suggested that α-actinin removing action of calpain rather than α-actinin cleavage would be expected to contribute to the weakening of Z-line.抄録全体を表示PDF形式でダウンロード (2413K) -
西村 昌彦, 信濃 晴雄1991 年 57 巻 6 号 p. 1141-1145
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーSince no means of sterilization is used throughout the manufacturing process of the salted and ripened squid meat product “Ika-Shiokara, ” the quality of the product depends on its associated microbial flora. In the early stage of “Ika-Shiokara” ripening using 10% salts, viable bacteria occured at a level of about 105%/ml of fluid, and then increased gradually with time. The dominant species of bacteria in “Ika-Shiokara” was Staphylococcus epidermidis, but Staphylococcus aureus which closely resembles S. epidermidis in many respects was not detected in all the stages of ripening process. The result suggests that growth of S. aureus was inhibited by some component of “Ika-Shiokara”. We paid attention to trimethylamine-N-oxide (TMAO) and trimethylamine (TMA) which were the characteristic components derived from squid meat. A high amount of TMAO (500-600mg/100ml) was detected in raw squid and in the early ripening process of “Ika-Shiokara”, and it inhibited the growth of S. aureus at this level of concentration but not of S. epidermidis in the nutrient broth.抄録全体を表示PDF形式でダウンロード (367K) -
坂田 泰造, 藤田 義之, 保本 洋之1991 年 57 巻 6 号 p. 1147-1152
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe bacterial strains belonging to the genus Saprospira were isolated from sea water samples in Kagoshima Bay and shrimp aquaculture ponds by using of the mixed culture with marine diatoms and double layer agar techniques. A Saprospira strain SS90-1 formed crater-like plaques on a lawn of Chaetoceros ceratosporum, which enlarged unlimitedly in the way of concentric cycles during an incubation period under illumination. The plaque formation by Saprospira SS90-1 was repressed with antibiotics including penicillin G, ampicillin, strepto-mycin, erythromycin, and tetracycline.抄録全体を表示PDF形式でダウンロード (1195K) -
黄 登福, 蔡 明容, 鄭 森雄, 今村小島 はるみ, 吉田 多摩夫1991 年 57 巻 6 号 p. 1153-1157
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーAttempts were made to elucidate the sublethal responses of common carp Cyprinus carpio exposed to the feed additive butylated hydroxytoluene (BHT). The common carp was admini-stered BHT at sublethal levels such as 0.2-1.6% in the diet for 14 weeks. The growth rate was not found to significantly decrease when common carp were fed a diet with supplements of BHT (p>0.05). However, the declining growth rate was observed to be most significant in the fish fed diet with supplement of 1.6% BHT from the 4th to 8th weeks (p<0.05). The symptoms of hepatopancreas enlargement were commonly found in the fish group fed diet with supplement of BHT. However, this symptom would be alleviated when the fish were switched to the BHT-free dietThe pathological symptoms in hepatopancreas induced by BHT were observed under microscope. The lesions indicated that the enlarged hepatopancreas had heavy steatosis, eosinophilia, and megalocytic hepatosis.抄録全体を表示PDF形式でダウンロード (829K) -
今村小島 はるみ, 吉田 多摩夫, 黄 登福, 蔡 明容, 鄭 森雄1991 年 57 巻 6 号 p. 1159-1164
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe absorption, distribution, and excretion of 14C-BHT in rainbow trout Oncorhynchus mykiss and common carp Cyprinus carpio were examined. The radioactivity was high, as measured by the method of whole-body autoradiography, in the gall bladder and adipose tissue of the two fish species injected intravenously. The radioactivity of the bile was high, as measured by the method of scintillation counting, only in the common carp administered orally. In the excretion profile of 14C-BHT in the bile of rainbow trout, the radioactivity was increasing with time, reached a maximum level at 4 days or one week and then decreased gradually. In the excretion profile of 14C-BHT in common carp, the radioactivity was quickly distributed in tissues at the beginning. After 4 days, the radioactivity was shown to decrease in bile, hepatopancreas, and kidney.抄録全体を表示PDF形式でダウンロード (609K) -
安藤 正史, 豊原 治彦, 志水 寛, 坂口 守彦1991 年 57 巻 6 号 p. 1165-1169
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーTo clarify whether the tenderization of fish muscle is due to the resolution of rigor, changes in the rigor index and breaking strength of muscle were examined as to plaice, parrot bass, yellowtail, carp, red sea-bream, striped grunt, tiger puffer, and rainbow trout. Upon storage at 5°C, most fishes reached maximal rigor within 24h after death. The maximal rigor continued within at least 72h after death and no resolution of rigor was observed within this period. Meanwhile, the breaking strength of muscle of all the fishes tested except for tiger puffer decreased sharply within 24h after death and then decreased gradually, although the values were markedly diverse between the fish species. These findings suggested that fish muscle tenderization proceeded independently of the rigor regardless of fish species.抄録全体を表示PDF形式でダウンロード (250K) -
小笠原 正志, 田中 宗彦, 長島 裕二, 田口 武1991 年 57 巻 6 号 p. 1171-1175
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe effect of added 1-ethyl-3-{3-(dimethylamino)propyl} carbodiimide (EDC) on thermal gelation of actomyosins from oval filefish and flying fish was studied by means of jelly-strength, ATP sensitivity, and SDS electrophoresis. The thermal jelly-strength of both oval filefish and flying fish actomyosins was enhanced by EDC treatment (0.1 and 0.2mg/mg protein). From SDS electrophoretograms it was shown that in EDC-treated actomyosins the decrease of myosin heavy chain and the formation of polymers were observed. The EDC-treatment for acto-myosins resulted in the decrease in the relative viscosity and the disappearance of ATP sensitivity. The EDC-treated actomyosins dissociated into myosin and actin in the presence of ATP. The desalting-aggregation ability of actomyosins disappeared with EDC-treatment.抄録全体を表示PDF形式でダウンロード (298K) -
Mohamed Kamal, 渡部 終五, 橋本 周久1991 年 57 巻 6 号 p. 1177-1184
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーChanges in myofibrillar ATPase activity of ordinary and dark muscle of sardine Sardinops melanosticta were examined in relation with rigor mortis progress and concomitant decline of muscle pH during 24h postmortem period, along with changes during 10 days of ice and frozen storage.
Myofibrillar Ca2+- and EDTA-ATPase activities, and Mg2+-ATPase activity in the presence of Ca2+ of sardine ordinary muscle clearly decreased when “rigor index”, a parameter of rigor tension, proceeded to 50% and muscle pH fell to around 6.4. Mg2+-ATPase activity in the absence of Ca2+ showed a slight decrease under the same conditions. On the other hand, the ATPase activity of the dark muscle started decreasing only at full rigor when muscle pH fell to 6.4 in almost all assay systems, except Mg2+-ATPase activity in the absence of Call which hardly changed during rigor mortis progress. A marked decrease in the activity was observed with both sardine muscles during extended ice storage of 10 days.
Both sardine muscles showed a large fall in myofibrillar ATPase activity, when stored frozen for 10 days. With either muscle, pH decreased down to 5.8-5.9 after one day and remained essentially unchanged thereafter.抄録全体を表示PDF形式でダウンロード (513K) -
川崎 賢一, 大泉 徹, 今野 久仁彦1991 年 57 巻 6 号 p. 1185-1191
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーThe effect of the triglyceride oxidation product (TGOP) of sardine oil on myofibrillar ATPase activities was studied.
Incubation of myofibrils with TGOP increased their Ca-ATPase activity and decreased their K-ATPase activity, which is very similar to the case of the thiol group modification of myosin with thiol reagents. Therefore, oxidation of some thiol groups near the ATPase active site of myosin was suggested.
Ca-ATPase activity enhancement upon TGOP addition was affected not only by the amount of TGOP added but also by its weight ratio to myofibrils. At an identical ratio of TGOP to myofibrils high TGOP concentration caused a large extent of Ca-ATPase enhancement. The TGOP-induced Ca-ATPase enhancement was completely suppressed by diluting a TGOP concentration with a buffer containing 2-mercaptoethanol.
The decay of the K-ATPase activity achieved by incubating myofibrils with TGOP was analyzed by assuming the two first order reactions; a quick decrease in the activity in an early phase was followed by a slower decrease. The first order reaction rate constant estimated from the negative slope of K-ATPase decay was proportional to the TGOP concentration. Therefore, quantitative interaction of TGOP with myofibrillar protein was concluded.抄録全体を表示PDF形式でダウンロード (512K) -
李 南赫, 加藤 登, 中川 則和, 照井 正三郎, 関 伸夫, 新井 健一1991 年 57 巻 6 号 p. 1193-1201
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーIt is already known as “setting phenomena” that the breaking strength of salt-ground meat from walleye pollack, pre-incubated at 30°C or below, increases remarkably when further heated at 90°C.On the other hand, the breaking strengths of salt-ground meat from jurel, preincubated at any given temperature between 20 and 60°C, hardly change upon heating at 90°C.
It is also known that the increase in breaking strength of the heated gel from walleye pollack is strongly correlated with the formation of cross-linked myosin heavy chain in it. However, this is not the case in the heated gels through pre-incubation at any temperature of salt-ground meat from jurel.
The water drip together with released protein isolated from the heated gel of walleye pollack, formed by preincubation at 30°C or below, is small in quantity, whereas those from the heated gels of jurel, formed at any preincubation temperature, are of relatively large quantity.抄録全体を表示PDF形式でダウンロード (1132K) -
岸 肇, 埜澤 尚範, 関 伸夫1991 年 57 巻 6 号 p. 1203-1210
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリー(1) Carp ordinary muscle contained TGase activity of 1.5unit per g of wet weight which was easily extractable with a solution at low ionic strength. The TGase, partially purified by DEAE-cellulose and gel filtration chromatographies, showed molecular weight of about 80, 000 and Ca2+-requirement for full activation. These properties characterize the enzyme as a “tissue” TGase.
(2) It was observed that rate and extent of TGase-catalyzed incorporation of MDC into myosin B and myofibrils were lower than those into acetylated casein.
(3) The reactivity of TGase on carp myosin B and myofibrils was fluctuated by the conformational alteration of the substrate proteins; the extent of MDC incorporation appeared to be 2.4-fold elevated in “soluble myosin B” than “insoluble myosin B” or myofibrils at identical amount of the enzyme.
(4) MDC was preferentially labeled on myosin heavy chain, actin, and troponin-T among the constituent proteins of myofibrils.抄録全体を表示PDF形式でダウンロード (785K) -
長崎 慶三, 内田 有恆, 石田 祐三郎1991 年 57 巻 6 号 p. 1211-1214
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーA monoclonal antibody GN-89 which recognizes the cell surface of Gymnodinium nagasaki-ense G-303-ax2 was obtained. It was reactive with nine strains of Gymnodinium nagasakiense (Gymnodinium Type-'65) and one strain of Gyrodinium sp., but not with Gymnodinium breve, Gymnodinium catenatum, Gymnodinium cf. nagasakiense (Gyrodinium cf. aureolum Hulbert), and some other genera of red tide algae by means of indirect fluorescent immunoassay. The antigen recognized by GN-89 seemed to be very hydrophobic, because GN-89 reacted with the antigen which was dissolved with diethyl ether but not with distilled water. The antigen had a polypeptide moiety, and the antigenic determinant was very heat-stable.抄録全体を表示PDF形式でダウンロード (1148K) -
内田 卓志1991 年 57 巻 6 号 p. 1215
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (824K) -
浜田 盛承1991 年 57 巻 6 号 p. 1217
発行日: 1991/06/25
公開日: 2008/02/29
ジャーナル フリーPDF形式でダウンロード (107K)
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