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Volume 59, Issue 9
Displaying 1-27 of 27 articles from this issue
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Yuji Uozumi, Hiroe Ohara1993 Volume 59 Issue 9 Pages 1469-1477
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSStatoliths of Nototodarus sloanii collected around New Zealand were examined to determine age. By comparing the increase in number of increments in statoliths with the number of days elapsed between sampling dates, the periodicity of the increment formation was validated. Estimated hatching month ranged from January to December. This result suggests that spawning occurs throughout the year. The relationship between statolith radius and dorsal mantle length suggests daily increment width must reflect squid growth well. Back-calculation was applied to compensate for the short sampling period. The differences in size at age are significant between the seasonal groups. The winter group is larger than the summer one between about 100 and 230 days old. This difference became smaller and finally the summer group became larger than the winter one at around 250 days old and after. It is suggested that differences in growth between the groups are consistent with seasonal changes in water temperature.View full abstractDownload PDF (1228K) -
Katsunori Tachihara, Ryousei Ebisu, Yasuo Tukashima1993 Volume 59 Issue 9 Pages 1479-1488
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe spawning, embryonic development, and morphological changes of the purplish amberjack Seriola dumerili were observed using specimens reared at the Aquaculture Research Laboratory of Nagasaki Prefectural Institute of Fisheries. Spawning was induced by intra-mascular injection of pituitary homogenate of the chum salmon Oncorhynchus keta and the genital hormone. Spawning occurred 36 hours after hormone treatment and 1, 400, 000 and 7, 000, 000 eggs were obtained in 1990 and 1991, respectively. The eggs were pelagic, spherical, and 1.05-1.20mm in diameter with a single oil globule of 0.22-0.28mm. The larvae hatched out 37 hours after spawning (W. T. 24.6°C). The newly hatched larvae were 2.7-3.1mm in length with 26 myotomes and a single oil globule. The yolk was mostly absorbed on the 4th day after hatching when the larvae attained 3.9mm in length and began to feed on rotifers. In the postlarval stage (6.3mm B. L.) the rudiments of ventral, dorsal, and anal fins began to develop and the vertebra was well ossified. In the juvenile stage (11.2mm B. L.) the membranous fins disappeared and fish gathered around drifting algae. Later in the juvenile stage (35.0mm B. L.) six or seven lateral bands appeared in the trunk as well as a band from the eye to the anterior part of the dorsal fin base. At a body length of 100mm, they reached the stage of young fish, while at a size of over 170mm in body length a yellow vertical stripe appeared from behind the operculum to the caudal peduncle. The smallest size of sexual maturation was thought to be about 600mm. Notochord or standard length at each growth stage was as follows: 2.7-3.9mm (yolk sac stage), 3.9-4.7mm (preflexion stage), 4.8-8.5mm (postflexion stage), 10-100mm (juvenile stage), 100-600mm (young stage), and over 600mm (adult stage).View full abstractDownload PDF (1288K) -
Kaworu Nakamura1993 Volume 59 Issue 9 Pages 1489-1493
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe gonopore site in the male Japanese spiny lobster Panulirus japonicus was identified by observations of the exuviae and histological preparations of the genital accessory apparatus as helicoid and articular condyle. The exuvial observation revealed that the gonopore opened at the bottom of a deep fold between the helicoid and condyle. The histology of the apparatus showed that it was structured without septa by superficial infolding of the cuticle, being distributed with connective tissue, haemocoelom, a few muscle fibers, and numerous nerve cords which suggested this apparatus to be a sensory organ. The inner wall of the distal was deferens at the gonopore had an epidermis instead of the epithelium which covered the proximal vas deferens.View full abstractDownload PDF (2963K) -
Kaworu Nakamura1993 Volume 59 Issue 9 Pages 1495-1501
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSTo define the role of the gill under cold air, common carp Cyprinus carpio acclimated to 17°C were exposed to 7°C air after being equipped with a hood, mask, plug, or bag, and their survival percentage was obtained. Secondly, carp acclimated to 7°C were exposed to 7°C air for 6.5-8h, 1) to analyze blood pH and gas, 2) to observe the gill histologically, and 3) to measure the activity of succinate dehydrogenase (SDHase) by methylene blue reduction for tissues including the gill of the specimens.
The survival percentage suggested that respiration via the gill occurred to a certain extent in cold air. This was supported by the results of blood gas analysis, histology, and SDHase measurement; in survivors, P02 showed an increasing tendency, and secondary lamellae as well as SDHase activity of the gill were revealed to be in an active condition.View full abstractDownload PDF (1929K) -
Hiroshi Kabasawa, Sadako Ooka-Souda1993 Volume 59 Issue 9 Pages 1503-1507
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSLight pulses of 15min to 6h were given to the hagfish Paramyxine atami, individuals displaying a free-running rhythm in locomotor activity under continuous darkness. The fish sensitively responded to the pulses; the active phase was promptly delayed or advanced when pulses were respectively given at the beginning or at the end of the duration of locomotor activity (α). The responses to light pulses in P. atami were characterized both by an initial phase-shift and by the changing period length (τ) in the ensuing active phase. In contrast, a closely related hagfish species Eptatretus burgeri responded to the light pulses by taking another stable course of the ensuing active phase with relatively constant τ
These results suggest that the sensitivity to light pulses in P. atami is higher than that in E. burgeri.View full abstractDownload PDF (321K) -
Eiichi Hasegawa1993 Volume 59 Issue 9 Pages 1509-1514
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe present investigation is an attempt to study the effect of light upon the behavior of fish in twilight vision, in which visual pigments in the outer segments of rods are strongly bleached. The optomotor reaction in albino and pigmented moenkhausia Moenkhausia sanctaefillomenae and rose bitterling Rhodeus ocellatus ocellatus were examined after light and dark adaptation.
Albino individuals were less reactive than pigmented ones, and the reaction was inferior in the case of light conditions with similar absorbance to the visibility by individuals.
These results suggest that light, similar to the visibility of fish, may possibly inhibit theonset of visual behavior. The method of utilization of fish lamps ought to be given all due consideration in light fishing.View full abstractDownload PDF (890K) -
Tohru Mukai, Noritatsu Sano, Kohji Iida, Seiji Sasaki1993 Volume 59 Issue 9 Pages 1515-1525
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe dorsal aspect target strength functions of ten species of fish collected by bottom trawl were measured at two frequencies. The average TS were calculated with respect to fish tilt angle distribution, which is assumed to have a mean value of -5 degrees and a standard deviation of 15 degrees. Both maximum TS and average TS were normalized with the fish length (L[cm]) squared and the 2/3 power of body weight (W[g]), namely,
TS=20logL+A, TS=20/3log W+B.
The results obtained were as follows:
(1) The target strength is generally higher at low frequency (25kHz) than at high frequency (100kHz). But for two species (Chub mackerel and Kandari), neither maximum nor average TS differ between low and high frequencies.
(2) The target strength of Silver pomfret, a bladderless fish, is clearly lower than that of bladder fish at two frequencies.
(3) The normalized TS (A or B) were calculated for the maximum TS (Amax or Bmax) and average TS (Aavg or Bavg). The results are: Amax = -63 - -59 dB, Aavg = -68 - -64 dB at low frequency; Amax = -65 - -59 dB, Aavg = -72 - -65 dB at high frequency, and Bmax = -52 - -45 dB, Bavg = -57 - -50 dB at low frequency; Bmax = -54 - -45 dB, Bavg = -60 - -52 dB at high frequency.View full abstractDownload PDF (643K) -
Ryosuke S. Isono, Yasuo Itoh, Hideaki Kinoshita, Katsutoshi Kido1993 Volume 59 Issue 9 Pages 1527-1533
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSOzonation of seawater produces residual oxidants which do not occur in fresh water. Most of them are bromine compounds, such as HBrO. In this study, the acute toxicity of these ozone-produced oxidants (OPO) to eggs and larvae of Japanese whiting, Sillago japonica, was tested under static-flow conditions of water with a temperature of 21.6 ?? 23.1°C, salinity 33.4 ?? 34.4‰, and pH 8.2 ?? 8.3. In analysis of the results, initial OPO concentrations measured as total residual chlorine were given importance since OPO disappeared gradually through contact with eggs or larvae. Based on the initial OPO concentration and normal survival rate, LC50's were estimated as 0.18mgC1/l (after 38h) and 0.23mgC1/l (after 24h) for eggs and larvae, respectively. Eggs were found to delay hatching. Many eggs exposed to near sublethal OPO stayed unhatched beyond the expected hatching time. After that, the death of these eggs was observed.View full abstractDownload PDF (495K) -
Shi-Yen Shiau, Tsai-Shen Hsu1993 Volume 59 Issue 9 Pages 1535-1537
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSL-ascorbic acid (C1), L-ascorbyl-2-sulfate (C2), and L-ascorbyl-2-monophosphate (C3-M) were each incorporated into a laboratory-made P. monodon diet. Diets were extracted by deionized water or by 5% meta-phosphoric acid and then subjected to standing for 0, 10, 20, 30, 40, 50, and 60min before high performance liquid chromatography (HPLC) analysis. Results indicated that the laboratory diet-making process destroyed about 67-75% of C1 and 21-24% of both C2 and C3-M. C2 and C3-M were stable before the analysis after either the water or meta-phosphoric acid extraction. On the other hand, a steady decline of 27.8, 41.1, 62.6, 73.9, 81.7, and 85.7% in C1 activity was found when extracted by water and allowed to stand for 10, 20, 30, 40, 50, and 60min, respectively, before the HPLC analysis.View full abstractDownload PDF (243K) -
Laura Pastoriza, Gabriel Sampedro1993 Volume 59 Issue 9 Pages 1539-1544
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSUsing frozen ray as a raw material and utilizing the muscle of this species as a principal component, three types of canned ray wing products with different packing liquids (garlic, pickle, and mustard sauces) have been developed as well as different canned pates similar to liver or salmon pate, and pates with pork meat and ray liver as ingredients. In the raw material, yield during processing, proximate analysis, and nitrogenous compounds were determined. In the final products, quality assessments were carried out during the storage period.
When the fish was cut up it was seen that the yield from the skinned wings corresponded to approximately 38% and that of the liver to 3% of the total fish weight. The proximate analysis of the muscle showed 2.07% protein nitrogen, 78% moisture content, and a fat content lower than 1%. The urea content reached the level of 2%. In the canned ray wing products it was observed that the intermuscular cartilage fraction was gelatinised after thermal treatment and subsequent storage, which increased the yield of the product and also avoided the necessity of filleting prior to canning. All the canned pates proved to have excellent textural, spreading, and sensory characteristics.View full abstractDownload PDF (385K) -
Akio Ozawa, Mikio Satake, Takao Fujita1993 Volume 59 Issue 9 Pages 1545-1549
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSLipids in the muscle of wild Kokanee salmon Oncorhynchus nerka adonis were analyzed and compared with those of cultured ones.
The lipid content of the cultured fish was higher than that of the wild ones. In both wild and cultured fish, the highest content was found in the ventral muscle, followed by the dorsal and tail muscles in that order. Triglycerides were more abundant in the cultured fish than in the wild ones, while phospholipids were vice versa. Triglycerides were richest in the ventral muscle in both wild and cultured fish. On the other hand, phospholipids were richest in the dorsal muscle in the wild fish, but in the tail muscle in the cultured ones.
Fatty acid composition of total lipids revealed that the wild fish had 16:1, 18:3n3, 18:4, 20:4n6, and 20:5, while the cultured ones had 16:0, 18:0, 18:1n9, 18:2, 20:1, and 22:6.View full abstractDownload PDF (352K) -
Huai-Jen Tsai, Hong-Ming Chen, Chu-Fang Lo1993 Volume 59 Issue 9 Pages 1551-1558
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA complementary DNA (564 base pairs) encoding for the mature region of the growth hormone (GH) of yellowfin porgy Acanthopagrus latus was amplified by polymerase chain reaction. This resultant DNA fragment, ypmGH cDNA, was inserted into baculovirus expression vectors, pAc7, under the control of the polyhedrin promoter. A 22 kDa protein, similar to that of authentic GH, was expressed intracellularly by the insect cell line of Spodoptera frugiperda 21-AE infected by recombinant baculovirus. This protein was reactive with rabbit polyclonal antibodies raised against natural chum salmon GH. Furthermore, a protein band with 22 kDa labeled with [35S] methionine was detected in tha lysate of cells infected with recombinant virus containing ypmGH eDNA. Results from treatment with tunicamycin, an inhibitor of N-link glycosylatíon, demonstrated that the N-glycosylation did not occur in the recombinant GH produced by this system.View full abstractDownload PDF (3232K) -
Seiichi Ando, Yusuke Mori, Kaworu Nakamura, Akira Sugawara1993 Volume 59 Issue 9 Pages 1559-1564
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe characteristics of lipid accumulation were examined in the muscle and liver from rive species of commercially important fish. The five species of fish could be clearly divided into two groups based on the lipid ratio of muscle to liver. Puffer and flounder fell into the group with a low lipid ratio, indicating that lipid was accumulated in their liver but not in the muscle. The lipid ratios of red sea bream, amberjack, and striped jack were much higher than those of puffer and flounder, and these fish accumulated lipid in their muscle as well as in their liver. Phosphatidylcholine and triglyceride were main lipid components in the muscle of fish with low and high lipid ratios, respectively. Triglyceride was also a main lipid component of the liver. In addition, considerable amounts of steryl ester were detected in the livers of flounder and puffer. Hepatosomatic index (HSI), the weight percentage of liver to body varied extensively in the five species. The highest and lowest HSI values were obtained in puffer and red sea bream, respectively. The lipid content of liver increased with HSI. The differences of lipid distributioh in muscle and liver were histologically observed. The tissues with high levels of lipid tended to possess numerous osmiophilic droplets. The histology of both muscle and liver showed specific distribution of osmiophilic droplets, supporting the results of chemical analysis of lipid.View full abstractDownload PDF (1389K) -
Seiichi Ando, Yusuke Mori1993 Volume 59 Issue 9 Pages 1565-1571
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSWe examined the lipoprotein features in the serum from puffer, flounder, red sea bream, amberjack, and striped jack in connection with the lipid levels of muscle and liver. Serum lipoproteins consisted of very low density lipoprotein (VLDL) and high density lipoprotein (HDL). HDL was a major lipoprotein in the five species. Species specific differences were found in the levels of VLDL. Considerable amounts of VLDL as well as HDL were present in the serum of flounder and striped jack, while the level of VLDL was extremely low in the serum of puffer. Flounder possessed unique VLDL whose main lipid was cholesteryl ester (CE) but not triglyceride (TG). The VLDLs other than that of puffer possessed apolipoprotein (apo) B- and A-I-like proteins as common components. In addition, the apolipoprotein with molecular weight (Mr) 75K was present in the VLDLs from flounder, red sea bream, and amberjack. The apolipoprotein with Mr 75K might be associated with the CE level of VLDL, and this apolipoprotein was absent in the VLDL of striped jack. The HDLs other than that of red sea bream possessed both apo A-I- and A-II-like proteins, while red sea bream possessed only apo A-I-like protein in its HDL. A close relationship was found between lipoprotein and muscle and liver lipid levels. Striped jack, with the highest level of muscle lipid, possessed the highest TG level of VLDL of the five species. Puffer, with the highest level of liver lipid, possessed the lowest level of lipoproteins. Lipoproteins synthesized in the liver seemed to be associated with the storage sites of lipid in fish.View full abstractDownload PDF (1104K) -
Takeshi Watanabe, Juadee Pongmaneerat, Shuichi Sato, Toshio Takeuchi1993 Volume 59 Issue 9 Pages 1573-1579
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe utilization of some alternative protein sources, i.e. defatted soybean meal (SBM), corn gluten meal (CGM), and meat meal (MM), as a partial replacement for fish meal was studied with rainbow trout having an average body weight of about 3g. The combination of those protein meals was used to replace 55, 64, 73, 82, and 91% of fish meal in the control diet containing 42% protein. Duplicate tanks of fish were fed to satiation at 15°C for 13 weeks.
Fish fed all the SBM, CGM, and MM combination diets produced a good growth rate, feed efficiency, and voluntary food intake comparable to the control group. Although the PER values of the experimental diets containing alternative protein sources were lower than that of the control, there was no marked difference in protein and energy retention except for the 91% fish meal replaced diet. However, this diet still seemed to be econom-ically beneficial as a relief for a short supply of fish meal. All the test proteins were well digested, assimilation values ranging from 91 to 93%. Hepatosomatic index and chemi-cal composition of the whole body did not vary from the control group.View full abstractDownload PDF (446K) -
Yang Wang, Kunio Kobayashi, Rizald Max Rompas, Yuji Oshima, Nobuyoshi ...1993 Volume 59 Issue 9 Pages 1581-1585
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSA study on the relation between the toxicity of fenitrothion (FS) and its metabolism in kuruma prawn Penaeus japonicus treated with piperonyl butoxide (PB) was performed to elucidate the cause of the remarkable reduction of FS toxicity in the prawn by PB treatment, using 14C labeled FS.
The PB treatment did not affect FS absorption by the prawn from media, but it considerably inhibited the biotransformation of FS to fenitrooxon (FO), i.e. the reduction of FS toxicity in the prawn by PB treatment was attributed to the decrease in FO formation. The minimum lethal concentration of FO in the prawn was presumed to be 10-14pmol/g, whereas that of FS was higher than 400pmol/g. According to the amounts of FS and its metabolites found in kuruma prawn treated with and without PB, the main metabolic pathway of FS in the prawn was presumed to be FS→FO→desmethylfenitrooxon→3-methyl-4-nitrophenol→its conjugates.View full abstractDownload PDF (331K) -
Takafumi Shikata, Toshiro Masumoto, Sadao Shimeno1993 Volume 59 Issue 9 Pages 1587-1591
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSThe effect of feeding rate on the hepatopancreatic enzyme activities and body composition in carp was studied by feeding a high protein diet at different feeding rates (100, 70, 30, and 0% of satiation). As the feeding rate decreased, so did the hepatopancreatic glycogen and crude fat contents, as well as serum triglyceride, phospholipid, cholesterol, glucose, free amino acid, and total protein levels. Serum free fatty acid level was higher in the 0% (starvation) group than in the other feeding groups. The activities of dehydro-genases in the pentosephosphate cycle and amino acid degrading enzymes showed decreasing tendencies as the feeding rate decreased, while almost no changes were detected in the activities of glucose-6-phosphatase, pyruvate kinase, and NADP-malate dehydrogenase. These results suggest that severe restricted feeding of a high protein diet depresses amino acid catabolism and lipogenesis and stimulates glycogen and fatty acid mobilization, but maintains glycolysis and gluconeogenesis.View full abstractDownload PDF (365K) -
Takeshi Suzuki, Yumiko Yoshie, Takaaki Shirai, Toshiyuki Hirano1993 Volume 59 Issue 9 Pages 1593-1597
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSChanges in dissolved carbohydrates from Gagome Kjemaniella crassifolia by gamma-irradia-tion and boiling were investigated. When pulverized Gagome was irradiated in the dry state, solubilized carbohydrate levels did not change at dose levels up to 50 kGy; however, the molecular weights of main components in the soluble matter decreased. The amount of dis-solved carbohydrates increased with dosage increments of gamma rays when irradiated in water, and their molecular weights decreased markedly. When Gagome was irradiated with a dose of 10 kGy in the dry state, and then boiled in water, the amounts of carbohydrates dissolved were markedly high at 80°C during 10-h boiling in comparison with those of the non-irradiated control. On the other hand, at 100°C no difference was observed after 4-h boiling. There was no significant difference in the quantity of dissolved carbohydrates or their molecular weights between irradiated and non-irradiated samples heated at 120°C for up to 12 h. From these results dietary fibers in Gagome were found to be extracted and degradated by irradiation.View full abstractDownload PDF (335K) -
Yasuhiro Funatsu, Hajime Hosokawa, Shoichi Nanbu, Ken-ichi Arai1993 Volume 59 Issue 9 Pages 1599-1607
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSWalleye pollack surimi without any additives was ground with 2.5% NaCl, 0.3% sodium polyphosphate, and 0 ?? 8% sorbitol. The salt-ground meat was incubated at 20°C and 40°C for setting, and again at 90°C for 20min to prepare setting-heating gel. Breaking strength (BS) and breaking strain (bs) of the setting and setting-heating gels with the cross-linking of myosin heavy chain (HC) were investigated as a function of setting time.
The values of BS and bs of the gels formed through setting with 0.8% sorbitol were higher than those of the gels formed by setting without sorbitol.
During the setting at 20°C, cross-linked myosin HCs with small molecular sizes in the gels with 8% sorbitol were formed in a large quantity as compared with those in the gels without sorbitol. However, in the setting at 40°C, the increase of cross-linked myosin HC in the gels with 8% sorbitol was in a small quantity and was nearly identical to that of the gel without sorbitol.
These results indicated that sorbitol in surimi affected the cross-linking of myosin HCs of the salt-ground meat and contributed to an improvement in gel formation of the meat.View full abstractDownload PDF (1032K) -
Michiko Takai, Koji Yamazaki, Yuji Kawai, Norio Inoue, Haruo Shinano1993 Volume 59 Issue 9 Pages 1609-1615
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSFour kinds of “ika-shiokara”, namely “akazukuri”, “kurozukuri”, “shirozukuri” and “kurozukuri (hakuhi)” were prepared with a combination of squid liver and/or ink and skinning treatment. Changes in chemical properties, VB-N, TMAO-N, TMA-N, free amino acids, water activity, and pH were compared among them during the ripening process. The addition of squid liver and ink and the skinning treatment played an important role in each “ika-shiokara”. The VB-N, water activity, and pH values decreased with each treatment. The amount of free amino acids was increased by the addition of liver at an early stage of ripening stage. The extent of the increase was noticeably accelerated in the absence of the skinning treatment. At that time, the amount of amino acids contributing to “umami” was also increased in the “akazukuri”. The above findings support the explanation that the “akazukuri” is flavored and the “kurozukuri” can be stored for a longer period.View full abstractDownload PDF (513K) -
Michiko Takai, Koji Yamazaki, Yuji Kawai, Norio Inoue, Haruo Shinano1993 Volume 59 Issue 9 Pages 1617-1623
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESS“Akazukuri”, “kurozukuri”, “shirozukuri” and “kurozukuri (hakuhi)” were prepared and comparisons made among them to detect differences in viable cell counts and microflora during the ripening process. The viable cell counts and the rate of bacterial growth were depressed by the addition of squid liver and/or ink and by the removal of skin. The squid ink had a particularly strong effect, and elongated the period of edibility. Staphylococcus epidermidis has never been isolated from “ika-shiokara” with the addition of ink, and was therefore isolated without ink. In the in vitro experiments, both S. epidermidis and S. aureus were inhibited by the extracts of heated squid ink. This finding suggests that there are heat stable inhibitory substances in the squid ink.View full abstractDownload PDF (489K) -
Yasuo Makinodan, Takayuki Nakagawa, Masao Hujita1993 Volume 59 Issue 9 Pages 1625-1629
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSWe attempted to discover whether cathepsins of squid Todarodes pacificus mantle muscle and liver have an effect on the textural change of ika-shiokara (salted squid preserves) during ripening was examined. The existence of cathepsin D-like, B-like, and L-like enzymes (D, B, and L) in the two tissues was ascertained from the effects of pH and inhibitors on the hydrolysis of hemoglobin, Z-Arg-Arg-MCA, and Z-Phe-Arg-MCA by enzyme preparations. Every muscle enzyme was almost completely inhibited in the presence of 7% (w/v) NaCl, whereas liver B and L were considerably stable even in 15% NaCl. During the ripening of shiokara made by mixing muscle and liver with the addition of 7% NaCl and 3mM NaN3, the specific activity of muscle B and L increased with the penetration of liver enzymes to the muscle, and a remarkable hydrolysis of myofibrillar proteins accompanying the organoleptic softening of texture was observed after 3 days' ripening by SDS-polyacrylamide gel electrophoresis. Under the present experimental condition, the effect of bacteria on the ripening of shiokara seemed to be negligible. From these results, the hydrolysis of myofibrils by liver cathepsin B and L seemed to be involved in the textural change of ika-shiokara during ripening.View full abstractDownload PDF (1135K) -
Katsuji Morioka, Yutaka Shimizu1993 Volume 59 Issue 9 Pages 1631
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (112K) -
Takeshi Suzuki, Kiyonori Nakai, Yumiko Yoshie, Takaaki Shirai, Toshiyu ...1993 Volume 59 Issue 9 Pages 1633
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (93K) -
Aditep Nontratip, Hideaki Yamanaka1993 Volume 59 Issue 9 Pages 1635
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (112K) -
Ryutaro Ueda, Haruo Sugita, Yoshiaki Deguchi1993 Volume 59 Issue 9 Pages 1637
Published: 1993
Released on J-STAGE: February 29, 2008
JOURNAL FREE ACCESSDownload PDF (104K) -
1993 Volume 59 Issue 9 Pages 1668
Published: 1993
Released on J-STAGE: April 21, 2008
JOURNAL FREE ACCESSDownload PDF (14K)
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