Acrolein and its related compounds react with m-Aminophenol to form fluorescent products under the acidic condition. Analytical method for Acrolein and its related compounds in the atmosphere was investigated emplying the above reaction. The recommended procedure is as follows: Measure 4 ml of the sample solution containing up to 1000 μM of Acrolein and its related compounds into a test tube with stopper. After 1 ml of a mixed reagent (100 mg of m-Aminophenol and 120 mg of Hydroxylamine hydrochloride are dissolved in 10 ml of 1 M HCl) and 1 ml of 5M HCl are added, the sample solution is maintained in a water bath at 100°C for 10 minutes. This solution is cooled to room temperature with water. The fluorescence intensity is measured on a fluorometer whose excitation and emission wavelengths are 350 and 505 nm, respectively. Hydroxylamine hydrochloride is a masking reagent. Some aldehydes and ketones were reacted with Hpdroxylamine hydrochloride to form oximes. Diketones as Acetylacetone and Glyoxal were masked remarkably. Acetaldehyde and Propanal, from which α, β unsaturated aldehydes were formed by Aldol condensation, were also masked remarkably. Crotonaldehyde, Methyl vinyl ketone, 2-Hexenal and Methacrolein were also masked. Very little masking effect was observed with Acrolein. When Sodium nitrite and Sodium sulfite were made to coexist more than 20 and 5 times with Acrolein respectively, these lead to a negative error for the determination. The calibration curve had a satisfactory linearity in the range of 0.05 to 3.0μM for Acrolein, and the detection limit was 0.05μM. The coefficient of variation was found to be within 2.90%. Lower αβ unsaturated aldehydes and ketones (Crotonaldehyde, Methyl vinyl ketone, 2-Hexenal and Methacrolein), would interfere the determination of acrolain.

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