It was found that the determination of ammonia using Nessler's reagent was seriously suffered from the interference of formaldehyde. Thus the comparison was made of the analyses of ammonis using Nessler's and pyridine-pylazolone (p-p) procedures. It was found that the p-p procedure was free from the interferance from the major air pollutants and gave considerably lower ammonia level than the Nessler's procedure. The procedures for the sampling of ammonia were also tested. Although the sampling into 1/50 N sulfuric acid gave considerably lower efficiency of sampling with prolonged sampling, the sampling on glass fiber filter impregnated with sulfuric acid provided the high efficiency of sampling even with the flow rate of sampling of as high as 15 l/min. The atmospheric concentration at mountain, coast, residential and business areas measured by p-p procedure mostly ranged from 5 to 15 μg/m³ and the maximum concentration of 30 μg/m³ occurred at Chuo University.

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Today most people recognize some relation between air pollution and prevalence of chronic bronchitis. However, as reports on prognosis of chronic bronchitis is still very poor, it is regarded as a mild case which annoys people merely with cough and sputum.
Here, we would like to discuss on the prognosis of chronic bronchitis and clarify actual effect of air pollution on human health, i. e. actual character of the prevalence of chronic bronchitis, which, we are afraid, seems to be made light of unjusty.
General population, 39, 207 over 40 years old, were taken as the objective for this investigation. These 39, 207 were idntical to those for the epidemiological study of chronic bronchitis (1962-1964) including Japanese version of standard questionnaire of British Medical Reaserch Council's Committee, Chest-X ray examination and respiratory function test.
The result obtained is as follows:
Death rate in the chronic bronchitis group is higher than the control group having neither cough nor sputum.
Difference between the above two death rate is remarkable in old age. and the observing time advanced.
Age adjusted death rate of respiratory disese including cor-pulmonale and emphysema in chronic bronchitis group is approximary five times greater than the death rate in the group having no cough and sputum.

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Suspended particulates data sampled by high volume air samplers at the six sites in Osaka City were statistically analysed.
The concentations of suspended particulate matter, sulfates and benzene soluble organic matter collected during 1966 to 1969 were log.normally distributed.
In industrial area, mean concentration of suspended particulates and sulfates were respectively 404 μg/m³ and 35.4 μg/m³.
Correlation between suspended particulate matter and sulfates, nitrates, benzene soluble organic matter concentrations were also analysed.

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