日本ハイパーサーミア学会誌
Online ISSN : 1881-9516
Print ISSN : 0911-2529
ISSN-L : 0911-2529
1 巻, 4 号
選択された号の論文の9件中1~9を表示しています
  • 畑山 巧, 加納 永一, 行岡 宗彦
    1985 年 1 巻 4 号 p. 115-129
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    heatshock(以下HSと略す)反応とは生物の体温が至適温度から数度上昇した時細胞においてみられる反応であり,一般に著明な一群のたんぱくの誘導,合成がおこる.特長的なことは,このHS反応は,細菌,酵母,粘菌,植物,昆虫,鳥類,哺乳類などあらゆる生物に共通して認められることである.また,この時産生される数種類のheat shock proteins(以下hspsと略す)のうち分子量60,000~70,000と80,000~90,000の2種の主なhspsは生物種や進化を通して,その構造が保存されている.さらにこのHS反応は,単にHSによってのみならず,アミノ酸アナグロ遷移金属・酸化剤・毒物などへの暴露や,無酸素状態からの回復,ウイルス感染,組織傷害などによっても同様の細胞の反応が認められる.これらのことから,このHS反応は種々のストレスから細胞を守るための基本的かつ原始的な反応であることが示唆される.
    HS反応は,1962年にRitossaによって,Drosophila(ショウジョウバエ)の唾線染色体にHSによりパフが誘導されていることを見い出されて以来,主としてDrosophilaを中心として研究がなされてきた.しかし,この数年来これらのHS反応が他の生物種においても共通して認められることが明らかとなり,種々の生物種において研究がなされてきている.一般にHSに反応して細胞において大きく2つのことがおこる.第一は今まで合成されていた正常のmRNAおよびたんぱくの合成の抑制であり,第二はHSにより誘導された新しいmRNAsの合成とそのたんぱく群(hsps)の特異的な合成である.これらの反応を理解するためには,何がこれらの反応を誘導するのか,また何が上述の2つのeventを制御するのか,またこのようにして産生されたheat shock protein(以下hspと略す)はどのような細胞機能をもつのかなどいろいろな問題が解明されねばならない.
    ここでは現在まで明らかにされてきたHS反応機構およびhspの生物学的役割などについて概説する.
  • 中嶋 和喜, 久住 治男
    1985 年 1 巻 4 号 p. 131-137
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    An agar phantom possessing a cavity, which simulated the rectum, was employed in the experiment to evaluate temperature distribution at RF-heating. In the phantom, the rising temperature around the cavity at 0 and 6 o'clock, where the prostate is situated, was rather low. The temperature, however, rose prominently when the cavity was filled up with a condom containing 5 % NaCl solution. This suggested that NaCl solution facilitated the heating current around the cavity. These observations are thought to be applicable in cases of other malignancies in cavity organs such as the esophagus and bronchus, as well as prostatic cancer.
  • 三好 憲雄, 福田 優
    1985 年 1 巻 4 号 p. 139-145
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    The uptake of hematoporphyrin derivative (HpD) by Ehrlich ascites cancer cells was investigated with measuring of the fluorescence intensity of HpD bound to the cells at various temperatures. It was shown that there are two steps in the HpD binding process by the analysis of the HpD fluorescence emission pattern. (1) Weak binding mode : the fluorescence peak appeared at 617 nm in the early reaction time below 25 °C. (2) Stable binding mode : the fluorescence peak appeared at 630 nm even in the early reaction time above 30 °C and also in later time below 25 °C. Increased amount of HpD molecule was incorporated into the cencer cells at higher temperature and it was considered that the HpD uptake is mainly due to facilitated diffusion. The Arrhenius plots of logarithmic values of the initial velocity and the reciprocal of the absolute temperatures in HpD-uptake reaction was biphasic of which inflexion point being at 28.7 °C. From the plots, the lower activation energy corresponded to the weak binding and the higher one to the stable binding mode. It was considered that critical temperature correlates with the phase change of the membrane lipid to which HpD molecules bind.
    Furthermore, it was found that the S and G2 (+M) phase cells incorporated larger amount of HpD than the G1 phase cells by the cytofluorometer determinations of the amounts of nuclear DNA (Hoechst 33258) and HpD on a single cells.
    Survival curve analysis of the Ehrlich cancer cells in hyperthermia with or without pre-treatment with HpD administration reveald that both incorporated and membrane-bound HpD have a mild protective effect against hyperthermia. Nevertheless, it was suggested that the combination therapy of cancer cells by laser radiation after HpD administration plus hyperthermia might be more effective than that of each single treatment.
  • 伊藤 正敏, 阿部 由直, 松澤 大樹, 佐藤 多智雄, 宍戸 郁郎, 大谷 茂盛
    1985 年 1 巻 4 号 p. 147-153
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    A needle type applicator using heatpipe is proposed for local intracavital or interstitial thermotherapy of malignant neoplasmas. Advantages of heatpipe are as follows : (1) rapid and high heat transfer rate (2) homogenious heating along needle length (3) easy temperature control. Its disadvantages are traumatic and short range of heat transfer in the transaxial direction. But the short range of heating may be a merit to preserve surrounding normal tissues from heat damages and several numbers of heatpipes inserted with appropriate arrdngement may control bulky tumors. Histological studies revealed gradual changes after heat therapy in the trans-axial plane. The tissue close to the heatpipe shows necrosis and between it and viable tumor tissue capillary dilatation with hemorrhage is found. The boundary of tissue damage is so clear that it supports existence of a breaking point in thermal damage in the tissue level.
  • 加藤 博和, 石田 哲哉
    1985 年 1 巻 4 号 p. 155-165
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    RF capacitive type heating has been regarded as a modarity of superficial heating. This heating modarity, however, can be also used for deep heating if some points are taken into account. In this paper, the features of this heating modarity are described and some approaches to the problems of deep heating are discussed.
  • 白土 博樹, 佐藤 隆之, 辻井 博彦, 入江 五朗, 内藤 紘, Tadashi Kamada
    1985 年 1 巻 4 号 p. 167-170
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    To shorten the treatment time of hyperthermia, pulse-heating with high temperature is expected to be as effective as long-time heating with lower temperature. An equipment to pulse out microwave was developed and the temperature distribution in the static phantom was investigated. Surface cooling unit made it possible to heat the deep portion without overheating the surface. The optimum temperature and the interval of pulse should be investigated further.
  • 加藤 和夫, 松田 甚一, 斉藤 義明
    1985 年 1 巻 4 号 p. 171-177
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    We will present a new RF capacitive heating method in order to heat a human deep seated tumor effectively. In our method, a human body is immersed in a water bath and dielectric plates are used to control the RF electric current distribution throughout the human body.
    From the computer simulations, it is shown that a deep seated tumor may be heated safely without generating dangerous hot spots, when a subcutaneous fat layer, bone, etc., are shielded electrically by these dielectric plates.
  • 村上 篤信, 古賀 成昌, 前田 迪郎
    1985 年 1 巻 4 号 p. 179-186
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    Sensitivity of tumor cells to heat is increased when heat is combined with chemotherapy. Two human colon cancer cell lines (WiDr Cell and COLO 205, cell) were implanted in nude mice and examined for the sensitivity to heat with or without chemotherapy. 1x107 tumor cells were subcutaneously implanted in the right leg of 4-to 6 week old male BALB/C nu/nu mice. Local heating was applied when the weight of the implatned tumor reached ca. 400 mg. A thermostatistically regulated water bath provided the heat. The anticancer drugs (1/3 of LD 50) were i. p. infused 3 times at 4 days intervals 1 hr before heating : mitomycin C, cis-diamminedichloroplatinum and 5 fluorouracil by 3, 4. 9 and 25 mg/kg body weight, respectively.
    The following results were obtained.
    1) No antitumor effect was obtained in both tumors by one 30-min thermal session at 42 °C.
    2) Two 30-min thermal sessions at 42 °C exerted a significant antitumor effect on COLO 205 tumor.
    3) At 43 °C, both tumors showed heat sensitivity in one 30-min thermal session.
    4) Heat at 42 °C plus MMC or CDDP produced significantly augmented effects on both tumors by one or two 30-min thermal sessions; heat plus 5 FU was also effectve by two 30-min thermal sessions.
  • 柳川 繁雄, 曽根 康博, 土井 偉誉, 山本 五郎
    1985 年 1 巻 4 号 p. 187-191
    発行日: 1985/12/01
    公開日: 2009/09/29
    ジャーナル フリー
    Surface overheating is a most frequent limiting factor in clinical application of RF capacitive heatig of deep seated tumors. The surface hot spots often occured around the margin of electrodes and were not prevented merely by perfusing cold water into the conventional pads attached to the electrodes. We have designed a new type coolig pad, 33 × 37 cm in size and 2 cm thick, which is put beneath the conventional pad and widely covers the skin surface. In the phantom experiments and clinical trials of deep hyperthermia using Thermotron RF8, the excessive surface heating around the margin of the electrodes completely disappeared by this new cooling pad.
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