Thermal Medicine(Japanese Journal of Hyperthermic Oncology)
Online ISSN : 1881-9516
Print ISSN : 0911-2529
ISSN-L : 0911-2529
Volume 8, Issue 4
Displaying 1-10 of 10 articles from this issue
  • Kenzo Ohtsuka, Yasushi Hayashi, Mitsuo Yamane, Hirotomo Hattori
    1992 Volume 8 Issue 4 Pages 241-274
    Published: December 01, 1992
    Released on J-STAGE: January 28, 2010
    JOURNAL FREE ACCESS
    When living cells are exposed to non-lethal heat shock, they acquire a transient resistance to a subsequent heat challenge as determined by the increase in cell survival. This phenomenon is termed thermotolerance. In addition, it has recently been demonstrated that non-lethal mild heat shock induced a transient thermotolerant state of cellular structures and functions such as protein and RNA synthesis, RNA splicing, cytoskeletons and cell morphology. On the other hand, a brief heat shock and other environmental stresses are known to induce a family of proteins, called heat shock or stress proteins (hsps). There is much evidence to support the correlation between the development of thermotolerance and the enhanced synthesis of heat shock proteins, but some reports do not concur. Recently, some hsps (especially hsp70) has been shown to have a molecular chaperoning activity involved in folding and unfolding of proteins and assembly and disassembly of protein complexes under normal growth temperature. Also, hsp70 and DnaK protein (prokaryotic homologue of eukaryotic hsp70) have been suggested to associate with heat- or stress-denatured (unfolded) proteins and repair (fold) them using the energy of ATP.
    In this review, we attempted to interprete the phenomenon of thermotolerance based on the putative functions of hsps (especially hsp70) which have a molecular chaperoning activity and a protein repair enzyme activity.
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  • Satoshi Harada, Tohru Yanagisawa
    1992 Volume 8 Issue 4 Pages 275-286
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    Anti-tumor effects of 5-FU, FT-207, and FT-207 + uracil against Meth A fibrosarcoma and Sarcoma-180 with two repetitions of 43°C hyperthermia were examined in vivo, in BALB/c mice.
    The anti-tumor effect was evaluated in terms of tumor growth inhibition by measuring the tumor size for 7 days, monitoring drug concentrations with HPLC and GC, and histopathological study. Additionally, decomposition from FT-207 to 5-FU by heat was tested at 37°C and 43°C.
    As for the tumor growth inhibition, the combination of hyperthermic treatment and FT-207, and FT-207 + uracil showed a synergistic effect, but 5-Fu not, to the both cell lines. With hyperthermic treatment, FT-207 + uracil inhibited tumor growth significantly (p<0.05) more effectively than FT-207. After the second hyperthermic treatment, a slightly lowered tumor response to each of drugs was also observed. After the first hyperthermic treatment, increase in the drug concentrations of FT-207 + uracil and FT-207 was observed, while, there was a substantial significant decrease in that of 5-FU. After the second application of hyperthermic treatment, the concentrations of all the drugs decreased. Pathologically, tumor revealed severe congestion under the treatment of hyperthermia. As for the decomposition from FT-207 to 5-FU by heat, a slight amount of FT-207 changed into 5-FU.
    In conclusion, hyperthermia in conjunction with drug therapy is considered to be effective as a cancer treatment. However, it has a tendency of being influenced by the membrane transport systems of the chemotherapeutic agents. And it is assumed that the frequent combination of the two kinds of treatment contradict the chemotherapeutic effect due to decrease the drug concentration by hyperthermia.
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  • Youko Hishikawa Itoh, Mariko Aihara, Hiroshi Hori, Takashi Oguri, Nobu ...
    1992 Volume 8 Issue 4 Pages 287-290
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    To confirm thermotolerance in blood coagulation system, some activities concerning blood coagulation were compared between first and second heating of rabbits.
    The first heating of rabbits showed the significant prolongation of APTT, decrease of coagulation factors and platelets as like DIC, but the second heating of rabbits did not show significant changes in coagulation system.
    From these results, we concluded that thermotolerance was induced also in blood coagulation system of the preheating animals.
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  • Jun-ichi Asaumi, Shoji Kawasaki, Koji Nishikawa, Masahiro Kuroda, Seir ...
    1992 Volume 8 Issue 4 Pages 291-299
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    An adriamycin (ADR) -resistant cell line derived from Ehrlich ascites tumor cells (wild EATC) was established in our laboratory. In this report, difference for thermosesitivity and thermotolerance between wild EATC and ADR resistant EATC were observed. ADR uptake and killing effect of ADR in thermoresistant-induced wild EATC in comparison with non-treated wild EATC also were evaluated.
    1) There was no difference for thermosensitivity between wild EATC and ADR resistant EATC.
    2) ADR resistant EATC showed bigger magnitude of thermotolerance than wild EATC.
    3) ADR uptake in thermoresistant-induced wild EATC showed no change in comparison with that in wild EATC. The killing effect of ADR also had no change in both cells.
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  • Yukio Ohizumi, Satoshi Imamiya, Hiroshi Maezawa, Tomoyuki Mori
    1992 Volume 8 Issue 4 Pages 300-308
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    Combining effect of hyperthermia and intratumoral injection of epinephrine and anti-cancer drugs was studied using Lewis lung carcinoma. Epinephrine injected intratumorously sensitized the effect of hyperthermia and the effect of PEP, DWA2114R, and MMC injected intratumorously. These anticancer drugs injected intratumorously also had synergistic effect with hyperthermia. The combining effect of them was the greatest and almost additive of the combined effect of anti-cancer drugs and hyperthermia and that of epinephrin'e and hyperthermia. The combining effect tended to be more prominent at lower temperature (40.5°C) than that at higher one (43.5°C), especially in DWA2114R. Number of lung metastases was not differect significantly among each treatment. These findings suggested that the combined treatment would be useful, especially in mild hyperthermia.
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  • Daisuke Watanabe, Michio Miyakawa, Yoshiaki Saitoh
    1992 Volume 8 Issue 4 Pages 309-317
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    The feasibility of the chirp radar-type microwave computed tomography as the non-invasive thermometry system for hyperthermia has been discussed. The temperature resolution which has never been measured accurately up to the present time is basically determined by the resolution in attenuation measurement of microwaves and the accuracy of the bolus temperature control system. By improving the experimental system including the temperature control system, we succeeded in imaging of the temperature change by 1°C.
    The time required for the measurement which is required about 100 minutes now must be reduced for the practical use of the microwave CT. In this paper, the possibility of data reduction has also been discussed by comparing the quality of CT images reconstructed from reduced amount of data. From the experimental results, it is concluded that the amount of data required to reconstruct the CT images, even in the case of temperature imaging, can be reduced to at least one half by keeping the spatial resolution of 1 cm and the temperature resolution of 1 °C.
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  • An Experimental Study Using Phantoms
    Masahiro Kuroda, Keiji Inamura, Seiji Tahara, Seiichi Mimura, Junichi ...
    1992 Volume 8 Issue 4 Pages 318-325
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    Simultaneous radiohyperthermotherapy (SRH) is a combined hyperthermia-radiation therapy in which radiation is given during heating. Mutual interference between the high energy radiotherapy system (Toshiba LMR-15A) and the 13.56MHz capacitive heating system (Omron HEH-500C) was examined using phantoms prior to clinical trials. Phantoms were irradiated and heated simultaneously at right angles. The energy and flatness of irradiation were measured using films and were not affected by the heating system within the range of clinical use. The temperature increase was measured with a thermocouple thermometer, and the temperature distribution was determined by liquid crystal thermometer. The high energy radiotherapy system did not affect the heating device set power, the temperature increase and distribution during simultaneous ti eatiiieiiL. This study clarified that these apparatuses work simultaneously without clinically significant mutual interference. SRH using these apparatuses can be safely applied to clinical study.
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  • In Vitro Study and Clinical Experience
    Masahiro Kuroda, Keiji Inamura, Eiichi Makihata, Seiji Tahara, Seiichi ...
    1992 Volume 8 Issue 4 Pages 326-335
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    Simultaneous radiohyperthermotherapy (SRH) is a combined hyperthermia-radiation therapy in which radiation is given during heating. An in vitro study was performed on NIH3T3 cells using 60Co γ-ray for irradiation and a water bath for heat treatment. The thermal enhancement ratio increased after simultaneous treatment compared to sequential treatment with heating for 90 min at 43°C and irradiation. Simultaneous treatment was more cytotoxic than sequential treatment with heating for 20 min at 45°C and irradiation of 6 Gy. A clinical trial was performed on a 57-year-old female with post-operative recurrence of rectal carcinoma. This is the first reported clinical case treated with true SRH in which external irradiation was administered during mid RE capacitive heating. Twelve SRH treatments were performed on the recurrent lesion at a frequency of twice a week for six weeks using the high energy radiotherapy system (Toshiba LMR-15A) and the 13.56MHz capacitive heating system (Omron HEH-500C). Average temperature at the time of irradiation in mid heating was 41.1°C. The patient received 60 Gy in 30 fractions over six weeks. There was a dramatic reduction in pain after treament. The tumor marker CEA level decreased after treatment. On CT images taken after treatment, the tumor site became a low density area which indicated necrosis. There were no side effects encountered. These results suggest that further clinical study of SRH should be performed to clarify its advantages.
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  • Keizou Yamamoto, Kenji Nagata, Toshiko Siga, Takashi Murata, Yoshimasa ...
    1992 Volume 8 Issue 4 Pages 336-344
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
    36 patients with inoperable malignant pelvic tumors were treated by local hyperthermia combined with radiotherapy. The purpose of this study is to evaluate the thermometry results and the antitumor effects of this treatment.
    The 36 patients consisted of 14 colorectal cancers, 6 uterine cancers, 7 metastatic bone tumors, 5 urinary bladder cancers and 4 ovarian cancers. Hyperthermia was performed by 8 MHz RF capacitive heating equipment, once or twice a week after irradiation, four to 10 sessions in total. All cases underwent radiotherapy (total dose, 2050Gy), and 14 patients received combination chemotherapy in an addition to radiothermotherapy.
    20 (55.6%) of the 36 patients could undergo intratumor thermometry by direct puncture. In 8 cases (40.0%) of these 20 patients, the maximum tumor core temperature (Tmax) could be elevated to more than 42°C, and the mean Tmax was 41.7± 0.3°C (40.044.7°C). There was a significant correlation between Tmax and electric output during heating. Any tumors could not be heated to more than 42°C using electric output under 600 watt. And electric output was limited by a painful sensation due to overheating of subcutaneous fat tissue.
    The local response rate based on tumor regression of all cases was 38.9% (CR 1, PR 13, NC 22). The tumor response was dependent on Tmax and the tumor size. The response rate was fairly higher in the higher Tmax (≥ 42°C) group than the lower Tmax (< 42°C) group (75% vs 25%). And greater response was observed in small tumors. On the contrary, large tumors over 10cm in diameter tended only to become necrotic, but not to become smaller.
    There was no clinically severe complication associated with hyperthermia in this trial. So this combination therapy is a very promising therapeutic modality for unresectable malignant pelvic tumors.
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  • Kosuke Ueda, Yasuhiko Masui
    1992 Volume 8 Issue 4 Pages 345-351
    Published: December 01, 1992
    Released on J-STAGE: September 29, 2009
    JOURNAL FREE ACCESS
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