This review describes the oligosaccharide structures of glycosphingolipids in invertebrates, focussing especially on Protostomia animals such as Arthropoda, Annelida, Mollusca, Nematoda, Brachiopoda, Bryozoa and Platyhelminthes, as exemplified primarily by our own studies. Glycosphingolipid fractionation is performed by adsorption on ion-exchange resin into neutral, acidic, polar, and zwitterionic types. In vertebrates, neutral glycosphingolipid sugars consist of Glc, Gal, GlcNAc, GalNAc, and Fuc. Invertebrate glycosphingolipids, on the other hand, contain not only these sugars but also Man, which is well represented in Mollu and Arthro series oligosaccharide core structures. Moreover, some invertebrate glycosphingolipids have been found to contain Xyl and various
O-methyl sugars. Although ganglioside has not been detected in Protostomia, other acidic glycosphingolipids containing uronic acid or inositol phosphate have been characterized. Polar glycosphingolipids containing phosphoethanolamine or aminoethylphosphonate have been studied. Finally, a group of zwitterionic glycosphingolipids containing phosphocholine have been discovered. Although studies undertaken on glycosphingolipids of invertebrates are rather limited so far, it is hoped that further research may lead to a molecular phylogeny based on glycosphingolipid structures.
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