All issues
Volume 154 (1988)
- Issue 4 Pages 315-
- Issue 3 Pages 215-
- Issue 2 Pages 95-
- Issue 1 Pages 1-
Volume 154, Issue 2
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
TAKASHI YOHKAICHIYA, HIROSHI HOSHIAI, SHIGEKI VEHARA, AKIRA YAJIMA1988 Volume 154 Issue 2 Pages 95-100
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSYOHKAICHIYA, T., HOSHIAI, H., VEHARA, S. and YAJIMA, A. Fibronectin Localization in the Mouse Embryo from the Two Cell Stage to the Morula Stage. Tohoku J. exp. Med., 1988, 154 (2), 95-100 - Among the characters of fibronectin, the adhesiveness may play an important role in embryo implantation. It is concluded that, using the direct and indirect immunofluorescence techniques, fibronectin was not found on the zona pellucida, but on the embryo surface even from the two cell stage. The results imply that (i) fibronectin is not an initial differentiation marker for the stage of endoderm, which used to be postulated, (ii) the surface fibronectin which has been produced at least from the two cell stage is covered by the zona pellucida to mask its adhesiveness during the oviduct transport, and (iii) after hatching in the uterus cavity the surface fibronectin facilitates the embryo implantation.View full abstractDownload PDF (1475K) -
MARIKO KAMBE, YASUSHI MITACHI, RYUNOSUKE KANAMARU, AKIRA WAKUI1988 Volume 154 Issue 2 Pages 101-110
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAMBE, M., MITACHI, Y., KANAMARU, R. and WAKUI, A. Responsiveness of Peripheral Blood Lymphocytes from Cancer Patients and Healthy Donors to Interleukin-2 (IL-2). Tohoku J. exp. Med., 1988, 154 (2), 101-110 - The proliferative response of the peripheral mononuclear cells (MNC) from cancer patients and healthy individuals to IL-2 was studied by use of the quantitative microwell assay of the3H-TdR incorporation into the cells in vitro. After the addition of phytohemagglutinin (PHA), MNC acquired the reactivity to IL-2 within 3hr, and reached a maximum after 12 to 17hr of incubation. Although the IL-2 response of PHA-activated MNC from cancer patient was lower than that from healthy donor, there was no significant difference in the kinetics of proliferation. The maximum response of PHA-activated MNC from both cancer patients and healthy donors to IL-2 was observed at 96hr and 84hr, respectively. When monocytes were removed from MNC, IL-2-associated growth of the remaining fraction of MNC was decreased to 40-70% in both cancer patients and healthy donors. Furthermore, monocytes from cancer patients did not affect the IL-2 responsiveness of lymphocytes from healthy donors, and vice versa. The number of T lymphocytes having IL-2 receptors (IL-2R) from cancer patients was lower than that from healthy donors. These facts indicated that the lower IL-2 response of MNC from cancer bearer was due to the decreased number of T lymphocytes possessing IL-2R, and not due to monocytes themselves.View full abstractDownload PDF (939K) -
RYUICHI NAKAMURA, SAYURI WATANABE, TAKETOSHI HANDA, ISAMU MOROHASHI1988 Volume 154 Issue 2 Pages 111-113
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, R., WATANABE, S., HANDA, T. and MOROHASHI, I. The Relationship between Walking Speed and Muscle Strength for Knee Extension in Hemiparetic Stroke Patients: A Follow-Up Study. Tohoku J. exp. Med., 1988, 154 (2), 111-113 - Walking speed and isokinetic strength of knee extension were examined in 18 hemiparetic stroke patients prior to, and 4 and 8 weeks after gait training. Although increase of the walking speed did not coincide with that of the isokinetic strength of the affected and non-affected sides, regression analyses revealed that the strength of the affected side was the primary determinant of the walking speed and the variance explained by it gradually increased with a period of the training.View full abstractDownload PDF (303K) -
KAZURO KAISE, NOBUKO KAISE, KATSUMI YOSHIDA, YOICHI ITAGAKI, YOSHINORI ...1988 Volume 154 Issue 2 Pages 115-124
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKAISE, K., KAISE, N., YOSHIDA, K., ITAGAKI, Y., KISO, Y., ARAI, T., YAMAMOTO, M., SAKURADA, T., SAITO, S. and YOSHINAGA, K. Properties of Thyrotropin (TSH) Binding to Plasma Membranes Prepared from Rat Fat Tissues. Tohoku J. exp. Med., 1988, 154 (2), 115-124 - Rat fat membranes were prepared from male Sprague-Dawley rats. At 37°C, TSH binding to rat fat membranes was rapid and unstable, although the binding reached a steady state after 2hr and unchanged up to 24hr at 4°C. The binding to rat fat membranes was significantly inhibited by 50mM NaCl and almost completely inhibited by 150mM NaCl. TSH binding to rat fat membranes was not affected by 10mM dithiothreitol (DTT) or 1mM diamide although the binding to human thyroid membranes was inhibited by 10mM DTT significantly. Conventional Scatchard analysis revealed a single class of binding site which had lower Ka value (2.6×108M-1) than that of high affinity binding site of human thyroid membranes (9.3×108M-1). Immunoglobulin G (IgG) from patients with Graves' disease inhibited the binding of TSH to rat fat membranes. A significant correlation was observed between the inhibiting activity of Graves' IgG measured with rat fat and human thyroid membranes (r=0.82, p<0.01). In conclusion, TSH receptors on rat fat menbranes were not identical to those on human thyroid membranes, but TSH receptor antibodies crossreacted with TSH receptors in rat fat tissue.View full abstractDownload PDF (882K) -
Pressor Response to Vasopressin and Norepinephrine in DOC-Salt Hypertensive and Prehypertensive RatsYASUYOSHI OUCHI, YOSHIO YAZAKI, RONG-CHI TSAI, TERUNAO ASHIDA1988 Volume 154 Issue 2 Pages 125-133
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOUCHI, Y., YAZAKI, Y., TSAI, R.-C. and ASHIDA, T. Pressor Response to Vasopressin and Norepinephrine in DOC-Salt Hypertensive and Prehypertensive Rats. Tohoku J. exp. Med., 1988, 154 (2), 125-133 - Pressor responses to arginine-vasopressin (AVP) and norepinephrine (NE) were studied in deoxycorticosterone (DOC)-salt hypertensive and prehypertensive rats. DOC-salt rats received weekly subcutaneous injection of DOC acetate (30mg/kg) and given 1% saline for drinking. Salt and control rats received injections of sesame oil and given 1% saline or tap water, respectively. On the 5th day (prehypertensive stage) and at 6th week (hypertensive stage) after treatment had started, pressor responses were studied by measuring changes in mean arterial pressure recorded from the iliac artery in response to i.v. injections of AVP or NE under urethane anesthesia. Pressor response to AVP was enhanced both in DOC-salt hypertensive and prehypertensive rats compared with that in salt and control rats. Pressor response to NE tended to be enhanced in DOC-salt hypertensive rats, however, the enhancement was not observed in the rats in prehypertensive stage. Enhanced pressor response to AVP in DOC-salt prehypertensive rats was not due to the structural change of vascular beds, because peripheral resistance in isolated hindlimb preparations was similar in the three groups. Thus, pressor response to AVP was enhanced even in the prehypertensive stage in DOC-salt rats and the enhancement might be involved in the pathogenesis of hypertension in DOC-salt rats.View full abstractDownload PDF (782K) -
SHIGERU ASAKI, HIROSHI SATO, TOSHIAKI NISHIMURA, SHUNJI OHKUBO, RIN YA ...1988 Volume 154 Issue 2 Pages 135-141
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSASAKI, S., SATO, H., NISHIMURA, T., OHKUBO, S., YAMAGATA, R., ITO, S., SAITO, Y., MIYAZAKI, S. and YAGINUMA, N. Endoscopic Diagnosis and Treatment of Dieulafoy's Ulcer. Tohoku J. exp. Med., 1988, 154 (2), 135-141 - Dieulafoy's ulcer often develops unmanageable severe gastrointestinal hemorrhage and sometimes takes a fatal course. In the past surgical operations were considered to be the only life-saving measure for this lesion. Since 1979, 46 lesions of Dieulafoy's ulcer in 45 cases with active bleeding from exposed blood vessels were treated during emergency endoscopy by the hemostatic method of pure ethanol injection. Transient hemostasis was obtained in all cases. Rebleeding occurred in 5 cases (11%) and pure ethanol injection was performed again. Hemostasis was obtained in all cases, but one case again had rebleeding due to an overlooked Dieulafoy's ulcer located in the gastric fundus. Emergent surgical operation was performed in this case. No case required elective surgery and no deaths were attributed to bleeding. Our method achieved complete hemostasis at a rate of 98% (44 cases) for Dieulafoy's ulcer. The pure ethanol injection method is one of the most effective hemostatic methods for the Dieulafoy's ulcer.View full abstractDownload PDF (766K) -
NAOKO MINEGISHI, MICHIO NAKAMURA, KEN SUZAKI, MASAHIKO TERASAWA, MASAY ...1988 Volume 154 Issue 2 Pages 143-148
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMINEGISHI, N., NAKAMURA, M., SUZAKI, K., TERASAWA, M., MINEGISHI, M., TSUCHIYA, S. and KONNO, T. Chronic Granulomatous Disease with Neutrophil Membrane Cytochrome b Deficiency: Demonstration by Immunochemical Staining with Monoclonal Antibody. Tohoku J. exp. Med., 1988, 154 (2), 143-148 -Cytochrome b deficicency in the peripheral granulocytes of two male patients with chronic granulomatous disease was demonstrated by an immunocytochemical assay using a monoclonal antibody, 7D5, against human neutrophil cytochrome b. A mosaic of cytochrome b positive and negative neutrophils, indicating a carrier state in an X-linked trait, was found in the mother of patient 1 but not in the mother of patient 2.View full abstractDownload PDF (2406K) -
TSUNEO CHIBA, RYOJI OHI, TAKASHI UCHIDA, YUTAKA KAYASHI, IZUMI MOCHIZU ...1988 Volume 154 Issue 2 Pages 149-156
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSCHIBA, T., OHI, R., UCHIDA, T., HAYASHI, Y. and MOCHIZUKI, I. Persistent Jaundice after Hepatic Portojejunostamy in Biliary Atresia: Are the Patients' Prognoses Determined within 3 Months after Surgery? Tohoku J. exp. Med., 1988, 154 (2), 149-156 - Eighteen patients with biliary atresia who showed persistent jaundice after hepatic porto-jejunostomy were studied clinically. It was revealed that: 1) Patients who had abnormally high preoperative values of laboratory data showed persistent jaundice; 2) high portal pressure occurred frequently in patients with persistent jaundice; 3) most patients of persistent jaundice had a history of postoperative cholangitis; 4) in contrast to jaundice-free patients, those with persistent jaundice tended to show elevation in gamma-globulin, TTT and gamma-GTP in the early postoperative period. These results suggest that for patients showing an upward tendency in these laboratory data after surgery, carefully management should be taken, even if the serum bilirubin level continue to decrease.View full abstractDownload PDF (635K) -
TATSUHARU OHNO, TADASHI KANOH, TAKAYO SUZUKI, TOHRU MASUDA, KAGEMASA K ...1988 Volume 154 Issue 2 Pages 157-172
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOHNO, T., KANOH, T., SUZUKI, T., MASUDA, T., KURIBAYASHI, K., ARAYA, S., ARAI, H. and UCHINO, H. Comparative Analysis of Lymphocyte Phenotypes between Carriers of Human Immunodeficiency Virus (HIV) and Adult Patients with Primary Immunodeficiency Using Two-Color Immunofluorescence Flow Cytometry Tohoku J. exp. Med., 1988, 154 (2), 157-172 - A variety of phenotypic abnormalities of peripheral blood lymphocytes from 8 HIV-carriers (HIVC), 6 patients with common variable Immunodeficiency disease (CVID), and 13 patients with selective immunoglobulin deficiency (SIgD) were compared using two-color flow cytometry. There was a close resemblance in phenotypic abnormalities between HIVC and the patients with CVID; i.e., increases in CD8+CD11-, Leu7+CD16- and CD3+DR+ cells, and decreases in CD4+Leu8+, CD4+Leu8-, Leu7+ CD16+ and Leu7-CD16+ cells. The increase in CD3+DR+ cells was due to an increase in CD8+DR+ cells. The CD4/CD8 ratio was inverted in both groups. A strong correlation coefficient (CC) was found only between the CD4/CD8 ratio and CD4+Leu8+ cells in HIVC, while CC was also high between the CD4/CD8 ratio and CD8+CD11- cells in CVID. The phenotypic abnormalities of the patients with SIgD were various and no significant difference was found against the control, except for an increase in CD4+Leu8+ cells and a decrease in CD4+Leu8- cells, which suggests heterogeneity of immunological deficits in this group. In severe immunodeficiency, ineffective killer cells appeared to be induced as a result of an adaptive change involved in recurrent or persistent viral infections, and Leu8 molecule may be concerned in the susceptibility of CD4+cells to HIV.View full abstractDownload PDF (1504K) -
SHUICHI BASAKI, YUTAKA IMAI, KEISHI ABE, MINORU NIHEI, NAOYOSHI MINAMI ...1988 Volume 154 Issue 2 Pages 173-183
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSASAKI, S., IMAI, Y., ABE, K., NIHEI, M., MINAMI, N., MUNAKATA, M., SASAKI, H., SEKINO, H. and YOSHINAGA, K. The Hypotensive Mechanism of Percutaneous Transluminal Dilatation (PTD) in Renovascular Hypertension Due to Bilateral Renal Artery Stenosis. Tohoku J. exp. Med., 1988, 154 (2), 173-183 - The hypotensive mechanism of percutaneous transluminal dilatation (PTD) in renovascular hypertension due to bilateral renal artery was investigated in two patients. Blood pressure was monitored continously before, during and after PTD by use of a new automated blood pressure monitoring device based on finger volume-oscillometry. Plasma renin activity was measured repeatedly before, during and after PTD. A hypotensive effect appeared immediately after PTD and blood pressure remained low in the following observation period without any hypotensive medication. In these cases, the hypotension was accompanied by a transient decrease in heart rate immediately after PTD. The hypotensive response to PTD was not parallel to the basal plasma renin activity, suggesting that the renin-angiotensin system is not necessarily involved in the maintenance of the hypertension before PTD. The autonomic nervous system seemed to play a certain role. Since the hypotension was accompanied by a transient decrease in heart rate immediately after PTD, the hypotension may be induced either by a decrease in sympathetic tone or by an increase in vagal tone at least just after PTD. It is hypothesized that these changes in autonomic nervous activity are mediated centrally through the renal afferent mechanism in response to rapid changes in renal hemodynamics induced by PTD.View full abstractDownload PDF (3035K) -
YOSHIKUNI FUJITA, KOUICHI KAWAJI, TATSUMI MORIYA, KIYOKAZU MATOBA, KOH ...1988 Volume 154 Issue 2 Pages 185-193
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUJITA, Y., KAWAJI, K., MORIYA, T., MATOBA K., ISHII, K., YAJIMA, Y. and OKABE, H. Role of Calcium in Suppression of Glucagon Release from Isolated Diabetic Rat Pancreata. Tohoku J. exp. Med., 1988, 154 (2), 185-193 -To investigate the role of extracellular Ca2+ (Ca2+) in suppression of glucagon release in diabetic animals, pancreata were isolated from streptozotocin-diabetic rats and perfused with a 15mM glucose solution containing one of the following: (1) Ca2+ 2.1mM and insulin 22μU/ml, (2) Ca2+ 2.1mM and insulin 110μU/ml, (3) Ca2+ 2.1mM and insulin (-) or (4) Ca2+ 0.2mM and insulin 110μU/ml. Although perfusion with 22μU/ml of insulin did not alter glucagon release, perfusion with 110μU/ml of insulin in the presence of Ca2+ and glucose significantly reduced the release of glucagon from 1.9±0.3ng/min to 1.2±0.3ng/min for the first 3min. The absence of insulin enhanced glucagon release from the baseline level of 1.0± 0.1ng/min to 1.6±0.3ng/min at 11min and to 1.4±0.2ng/min at 21min. Deprivation of Ca2+ in the perfusate also enhanced glucagon release from the baseline level of 0.8±0.1ng/min to 1.2±0.3ng/min for the first 4min. It is concluded that insulin suppresses glucagon release and Ca2+ is needed for the suppression of glucagon release in the presence of both insulin and glucose in streptozotocin-diabetic rat pancreata.View full abstractDownload PDF (791K) -
Induction of the CD4-8+ Suppressor Phenotype in CD4+8+ Human Thymocytes by Phorbol Myristate AcetateYUKIO SAKIYAMA, AKIHITO ISHIZAKA, TOHRU WATANABE, TADASHI ARIGA, SHUZO ...1988 Volume 154 Issue 2 Pages 195-203
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSAKIYAMA, Y., ISHIZAKA, A., WATANABE, T., ARIGA, T. and MATSUMOTO, S. Induction of the CD4-8+ Suppressor Phenotype in CD4+8+ Human Thymocytes by Phorbol Myristate Acetate. Tohoku J. exp. Med., 1988, 154 (2), 195-203 -Human thymocytes were separated according to differential agglutination by peanut lectin (PNA). In the fractions obtained, the distribution and quantitative expression of CD antigens was determined by two-color fluorescence flow cytometry. A major population of CD4+8+ and minor populations of CD4+8- and CD4-8+ cells existed in unfractionated thymocytes. Agglutination of human thymocytes by PNA led to a highly effective enrichment of the CD4+8+ phenotype in the PNA+ thymocytes, and left three distinct phenotypes, CD4+8+, CD4+8-, and CD4-8+, in the PNA- thymocytes. After treatment of the CD4+8+ cells in both PNA+ and PNA- fractions for 20hr with phorbol 12-myristate 13-acetate (PMA), 60%-90% of the cells expressed the CD4-8+ phenotype, whereas the CD4+8+ phenotype was decreased to 1%-3% of the population. In addition, pretreatment of both PNA+ and PNA- thymocytes with PMA induced suppressor activity in these cells, as shown by inhibition of immunoglobulin secretion by pokeweed mitogen (PWM)-stimulated peripheral blood lymphocytes (PBL).View full abstractDownload PDF (831K) -
TETSUYA NAKAMURA, SHUICHI ICHIKAWA, TETSUO SAKAMAKI, MASAO FUJIE, ATSU ...1988 Volume 154 Issue 2 Pages 205-213
Published: 1988
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKAMURA, T., ICHIKAWA, S., SAKAMAKI, T., FUJIE, M., MAGI, A., KURASHINA, T. and MURATA, K. Plasma Levels of Atrial Natriuretic Peptide in Patients with Borderline and Essential Hypertension. Tohoku J. exp. Med., 1988, 154 (2), 205-213 - Plasma levels of atrial natriuretic peptide (ANP) were measured in outpatients with borderline hypertension (n=15) and essential hypertension (n=13) and in normotensive subject (n=11). There were no significant differences among the three groups in age, serum protein, albumin, or electrolyte levels, plasma renin activity (PRA), or plasma concentrations of aldosterone and cortisol. The plasma ANP levels in the normotensive, borderline hypertensive, and essential hypertensive subjects were 36±6pg/ml ( mean±S.E.), 64±11pg/ml, and 82±14 pg/ml, respectively. The levels in the essential hypertensive subjects were significantly (p<0.05) higher than those in the normotensives. In both borderline and essential hypertensives (n=28), the plasma ANP levels were significantly correlated positively with systolic blood pressure (r=+0.385, p<0.05), and negatively with PRA (r=-0.484, p<0.05) and serum total calcium (r=-0.516, p<0.01). These results suggest that the elevation of circulating ANP in hypertensives is involved in the pathogenesis of hypertension.View full abstractDownload PDF (813K)
- |<
- <
- 1
- >
- >|