巻号一覧
155 巻 (1988)
- 4 号 p. 305-
- 3 号 p. 213-
- 2 号 p. 107-
- 1 号 p. 1-
155 巻, 1 号
選択された号の論文の11件中1～11を表示しています
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HIROMI OGURA, KENZABURO TANI, HITOSHI KANNO, TAKAYUKI MORISAKI, MUNEYU ...1988 年 155 巻 1 号 p. 1-9
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーOGURA, H., TANI, K., KANNO, H., MORISAKI, T., ITO, M., KUBONISHI, S., MIYAZAKI, K., TAKEUCHI, T., FUJII, H. and MIWA, S. Molecular Analysis of Lesch-Nyhan Syndrome Found in Japan. Tohoku J. exp. Med., 1988, 155 (1), 1- 9-Molecular analysis of four unrelated patients with Lesch-Nyhan (L-N) syndrome was performed. All four cases had typical clinical features of L-N syndrome, and the activities of hypoxanthine-guanine phosphoribosyltransferase (HPRT) were absent. No structural gene abnormalities were found by Southern blot analysis in all cases, and a decrease of HPRT mRNA was not detected by dot blot analysis in two of the four cases. However, HPRT enzyme proteins were detected by Western blot analysis in all cases. Our results showed that the production of immunologically reactive but enzymatically inactive mutant HPRT protein was pathogenic for all four patients.抄録全体を表示PDF形式でダウンロード (1432K) -
KUNITOSHI NAKAGAWA, KIYOAKI OUCHI, SHUJI MATSUBARA, MASANORI SUZUKI1988 年 155 巻 1 号 p. 11-21
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーNAKAGAWA, K., OUCHI, K., MATSUBARA, S. and SUZUKI, M. Significance of Activation of Reticuloendothelial Function after Hepatectomy in Cirrhotic Rats. Tohoku J. exp. Med., 1988, 155 (1), 11-21-Effect of hepatectomy on the prognosis of cirrhotic rats prepared by oral administration of thioacetamide was studied from the standpoint of the reticuloendothelial function and energy metabolism of the liver. OK-432, a streptococcal preparation, was used to activate reticuloendothelial functions. Administration of OK-432 to cirrhotic rats prior to 70% hepatectomy significantly prevented the elevation of serum GOT, GPT and LDH, the prolongation of blood coagulation and the decrease of serum complement level. Hepatic ATP synthesis and RNA content were significantly increase by the use of OK-432. These findings suggest that activation of reticuloendothelial functions at the time of massive hepatectomy in cirrhotic rats may diminish hepatic injury, maintain serum complement level, and improve protein synthesis of the liver.抄録全体を表示PDF形式でダウンロード (1360K) -
SHINICHI OKUYAMA, HITOSHI MISHINA1988 年 155 巻 1 号 p. 23-39
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーOKUYAMA, S. and MISHINA, H. Cancer Incidence in the Population of Nagasaki City 30 Years after Atomic Bombing. Tohoku J. exp. Med., 1988, 155 (1), 23-39-In order to attest the evolutionary hypothesis of non-epithelial- epithelial tumor shift (Okuyama and Mishina 1986), the published cancer incidence data for the population of Nagasaki City during the period of 1973-1977 was studied along with appropriate control populations of Fukuoka City, and Miyagi and Osaka prefectures. Significant increments of non-epithelial tumors including leukemias, and epithelial tumors such as cervical cancers and choriocarcinomas were observed. An inference was made to the post-atomic bomb radiation carcinogenesis that different organ systems responded to that irradiation with different oncogenic patterns: Neoplasms of the pre-vertebral organs (non-epithelial organ systems) were the first to react and tapered long (curve type I); Tumors of the vertebral but pre-mammalian (those of the digestive tract, respiratory and thyroid) could have formed two separate peaks, one for the early and high dosage and the other for the late and low dosage (II); With breast cancer or the mammalian symbol tumor, there was a single peak reflecting the cohort of reproductives (III). The evolutionary and phylogenetic progression could have taken place from I through III. Thus, cancer incidence analysis may be helpful in deciphering the atomic bomb radiation carcinogenesis.抄録全体を表示PDF形式でダウンロード (1662K) -
TOHRU IKEUCHI, MINEHIKO YOKOYAMA, KENZO OHTSUKI, MINEHIKO YOKOYAMA1988 年 155 巻 1 号 p. 41-56
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーIKEUCHI, T., YOKOYAMA, M. and OHTSUKI, K. Characterization of a Polypeptide-Dependent Membrane Protein Kinase that Specifically Phosphorylates NS Protein of Vesicular Stomatitis Virus in Vitro. Tohoku J. exp. Med., 1988, 155 (1), 41-56-Phosphorylation of membrane-associated proteins by protein kinases in the membrane fraction from HeLa S3 cells was rapidly increased when the cells were infected with vesicular stomatitis virus (VSV). SDS-PAGE followed by autoradiography revealed polypeptides with molecular sizes of Mr. 53, 000, 44, 000, 42, 000, 35, 000, 30, 000 and 27, 000 in the kinase fraction from uninfected cells to be highly phosphorylated. Virus-coding NS protein (Mr. 40, 000) was phosphorylated when the membrane fraction from virus-infected cells was incubated with [γ-32P]ATP in the presence of histone H1 and Mg2+ Under these conditions, histone H1 functioned as a stimulator for NS protein phosphorylation by the kinases. One (kinase III) of the membrane-associated kinases was partially purified from HeLa S3 cells using FPLC (type Mono Q) after DEAF-cellulose column chromatography. The enzymatic properties of kinase III were similar to those reported for a Polypeptide-dependent protein kinase (protein kinase P), because (a) both kinases highly phosphorylated β-casein, although no phosphorylation was observed with histories; (b) several endogenous substrates from HeLa S3 cell membrane were phosphorylated by the kinases in the presence of basic proteins, such as histones, protamine and poly-Lys; (c) their activity was insensitive to a low concentration (19μg/ml) of heparin, which highly inhibited casein kinase II activity; and (d) the kinases were extractable from the plasma membrane using Triton X-100. In addition, provided evidence suggests that kinase III may play an important role in an early stage of VSV replication through its specific phosphorylation of NS protein and membrane proteins in virus infected cells.抄録全体を表示PDF形式でダウンロード (2105K) -
YOSHIKATSU KIKUTA, KANAKO SANJO, KATSUO NAKAJIMA, ITSUKI ASHIZAWA, MOT ...1988 年 155 巻 1 号 p. 57-70
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーKIKUTA, Y., SANJO, K., NAKAJIMA, K., ASHIZAWA, I. and OJIMA, M. Primary Aldosteronism in Childhood Due to Primary Adrenal Hyperplasia. Tohoku J. exp. Med., 1988, 155 (1), 57-70-We present an unusual case of primary aldosteronism in childhood. A 9-year-old boy had hypertension, hypokalemia, hyporeninemia and hyperaldosteronism. Dexamethasone administration decreased plasma aldosterone transiently but failed to correct the hyperaldosteronism, excluding dexamethasone-suppressible hyperaldosteronism. Plasma aldosterone decreased with upright posture and showed a circadian rhythm. Spironolactone treatment normalized blood pressure and serum potassium and lowered aldosterone secretion. During the studies, plasma aldosterone correlated with serum cortisol but not with plasma renin. Preoperative results indicated that this patient presented the functional features of aldosteronoma. Adrenal computed tomography, scintigraphy and left venography were not diagnostic of adrenal lesions. The left adrenal venous sampling showed hypersecretion of aldosterone from the left adrenal gland. The left adrenalectomy revealed micronodular hyperplasia but resulted in a prompt and sustained reversal of hypertension and hyperaldosteronism. These findings suggest that primary aldosteronism in this patient resulted from primary adrenal hyperplasia. Thus, adrenal hyperplasia is a heterogenous group of disorders and carefully selected studies allow prospective selection of appropriate treatment.抄録全体を表示PDF形式でダウンロード (1310K) -
HIROSHI NAKAMURA, AKIRA TAKAGI, AKIKO TSUDA, TAKESHI MOMOTSU, SEIKI IT ...1988 年 155 巻 1 号 p. 71-80
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーNAKAMURA, H., TAKAGI, A., TSUDA, A., MOMOTSU, T., ITO, S. and SHIBATA, A. Residual Beta-Cell Function and Metabolic Stability in Insulin-Dependent Diabetes Mellitus. Tohoku J. exp. Med., 1988, 155 (1), 71-80-Using the modified sensitive method of measurement of urinary C-peptide immunoreactivity (CPR) excretion, we studied whether positive correlation between residual beta-cell function and metabolic consequence is present in insulin-dependent diabetes mellitus (IDDM) or not. After urinary CPR excretions were measured for 3 days in 40 IDDM, they were divided to three groups: group A, urinary CPR excretion <1μg/day (n=16); group B, 1-4μg/day (n=15); group C, ≥4μg/day (n=9). All subjects were treated with intensive insulin therapy for 1-2 months, and 1) fasting blood glucose (FBS) for one week, 2) 24-hr urinary sugar excretion (US) for one week, 3) M-value for daily variation of blood glucose, and 4) hemoglobin A1 (HbA1) were measured before and after intensive insulin treatment. And, we calculated mean and standard deviation (S.D.) of FBS and US. After intensive insulin therapy, a significant difference of the S.D. of FBS was seen between group A and group B (p<0.01). And, a significant difference of M-value was found between group A and group B (p<0.05). As significant differences were observed between group A and group B, which could not be divided into two groups by conventional CPR measurement, it seems likely that metabolic stability in IDDM mainly result from minimal remaining residual beta-cell function.抄録全体を表示PDF形式でダウンロード (892K) -
HIROSHI NAGANUMA, MOTOKO OJIMA, NOBUAKI SASANO1988 年 155 巻 1 号 p. 81-96
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーNAGANUMA, H., OJIMA, M. and SASANO, N. 11β-Hydroxylase in Mitochondrial Fractions of Functioning and Non-Functioning Adrenocortical Tumors. Tohoku J. exp. Med., 1988, 155 (1), 81-96-The activity of 11β-hydroxylase was investigated in the mitochondrial fractions of 19 surgically resected adrenocortical tumors associated with Cushing's syndrome (4 adenomas and 2 carcinomas), primary aldosteronism (8 adenomas), adrenogenital syndrome (AGS) (2 carcinomas) and no hormonal symptoms (3 adenomas). Five adrenal cortices from patients with mammary carcinoma, renal cell carcinoma and pheochromocytoma were used for the normal control. The activities of 11β-hydroxylation of deoxycorticosterone and of 11-deoxycortisol in the control adrenal cortices were 0.66-2.16 pmole/mg protein/min (mean: 1.28 pmole/mg protein/min) and 0.25-0.77 pmole/mg protein/min (mean: 0.56 pmole/mg protein/min), respectively. The activities in adenomas and carcinomas associated with Cushing's syndrome were in the range of normal control. The activities in aldosteronomas were significantly higher in 4 cases than those of the normal control and in the range of the normal control in 4 cases, suggesting that the higher activity of 11β-hydroxylase is one of the important factors causing mineralocorticoid excess. The activities in adrenocortical carcinomas with AGS were significantly lower, corroborating clinical findings of androgen excess with suppressed production of mineralocorticoid or glucocorticoid. The activities in two cases of nonfunctioning adenomas were in the range of the normal control, but the third case showed higher activity than the normal control. These results show that the abnormal activity of mitochondrial 11β-hydroxylase exists in some aldosteronomas, non-functioning adrenocortical adenomas and carcinomas with AGS.抄録全体を表示PDF形式でダウンロード (4655K) -
TOKUTARO SATO, HISAO SASAKI, RYOICHIRO WATANABE, KAORU YOSHINAGA1988 年 155 巻 1 号 p. 97-100
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーSATO, T., SASAKI, H., WATANABE, R. and YOSHINAGA, K. Enhancement of Pentose Phosphate Pathway in Vascular Intima from Diabetic Rabbit. Tohoku J. exp. Med., 1988, 155 (1), 97-100-Activity of pentose phosphate pathway in the intima grown inside vascular prosthesis was studied in alloxan diabetic rabbits. Ratio of 14CO2 production from 1-14C-glucose and 6-14C-glucose was 7.8 in the nondiabetic group and 78.9 in the diabetic group. Statistically positive correlation was found between the ratio and the levels of blood glucose or plasma triglyceride. These results suggest that pentose phosphate pathway in the vascular intima is active, and enhanced to a great extent in diabetes.抄録全体を表示PDF形式でダウンロード (2614K) -
NORIO SHIBUYA, TATSUO OHTA, HIROSHI SAKAI, KAZUO ENDOH, MASAHARU YAMAM ...1988 年 155 巻 1 号 p. 101-102
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーSHIBUYA, N., OHTA, T., SAKAI, H., ENDOH, K. and YAMAMOTO, M. Mutagenicity of the Ash of Rice Straws by Ames' Test. Tohoku J. exp. Med., 1988, 155 (1), 101-102-Mutagenicity of fly ashes and bottom ashes of rice straw and rice husk was assayed by Ames' test. With respect to rice-straw ash, the extract from the fly ash was found to be more mutagenic than that from the bottom ash. In the case of rice husk, the mutagenicity of extract from the bottom ash was stronger than that from the fly ash. The extract from rice-husk bottom ash showed the strongest mutagenic activity among the four.抄録全体を表示PDF形式でダウンロード (184K) -
NOBUO OHYA, JYONGSU HUANG, TOSHIHARU FUKUNAGA, HIROHISA TOGA1988 年 155 巻 1 号 p. 103-104
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーOHYA, N., HUANG, J., FUKUNAGA, T. and TOGA, H. Mouth Pressure Curve on Abrupt Interruption of Airflow during Forced Expiration. Tohoku J. exp. Med., 1988, 155 (1), 103-104-The mouth pressure curve after abrupt interruption during forced expiratory maneuver was investigated to evaluate the collapsing state of the airway downstream to the choke point. Immediately after the airflow interruption at the mouth by means of the electromagnetic valve, the mouth pressure suddenly increased (1st phase), followed by a slower rise (2nd phase) within about 100msec until the pressure reaches the alveolar pressure. The pleural and alveolar pressures remained constant during this process. It was evidenced that, from point of view of mean flow, the airflow flowed at a rate of Vmax through the choke point during the second phase. Thus, it is strongly suggested that the choke point remained at the same point during the 2nd phase. From these results, the 2nd phase of the mouth pressure is expected to represent the specific characteristics for the downstream airway.抄録全体を表示PDF形式でダウンロード (165K) -
NOBUO OHYA, JYONGSU HUANG, TOSHIHARU FUKUNAGA, HIROHISA TOGA1988 年 155 巻 1 号 p. 105-106
発行日: 1988年
公開日: 2006/08/31
ジャーナル フリーOHYA, N., HUANG, J., FUKUNAGA, T. and TOGA, H. Airway Pressure-Volume Curve Estimated by Flow Interruption during Forced Expiration. Tohoku J. exp. Med., 1988, 155 (1), 105-106-The pressure-volume characteristics of the airway downstream to the choke point was estimated with abrupt interruption of airflow at the mouth during forced expiration. The pressure was measured at the mouth and the volume was given by the maximum flow immediately before interruption multiplied by interruption time. The compliance of the airway downstream to the choke point, i.e., a slope of the pressure-volume curve, was 1.04ml/cmH2O at 50% FVC, and increased in lower lung volumes, in 7 healthy subjects. Whereas, in a case of tracheo-bronchopathia osteochondroplastica, its value decreased markedly.抄録全体を表示PDF形式でダウンロード (160K)
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