巻号一覧
157 巻 (1989)
- 4 号 p. 289-
- 3 号 p. 185-
- 2 号 p. 83-
- 1 号 p. 1-
157 巻, 2 号
選択された号の論文の11件中1～11を表示しています
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KAZUYA KITAMURA, TOSHIO TAKAHASHI, TOSHIHARU YAMAGUCHI, TAKASHI YOKOTA ...1989 年 157 巻 2 号 p. 83-93
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーKITAMURA, K., TAKAHASHI, T., YAMAGUCHI, T., YOKOTA, T., NOGUCHI, A., AMAGAI, T. and IMANISHI, J. Immunochemical Characterization of the Antigen Recognized by the Murine Monoclonal Antibody A7 against Human Colorectal Cancer. Tohoku J. Exp. Med., 1989, 157 (2), 83-93-The nature of the antigen recognized by the murine monoclonal antibody A7 (Mab A7) against human colorectal carcinoma was investigated using immunochemical and biochemical techniques. Binding activity of 125I-labeled Mab A7 was examined using various human cancer cell lines. Mab A7 gave the highly specific binding to colon cancer cell lines, SW1116 and WiDr, and gave only a very weak or no reactivity to gastric cancer cell lines, pancreas cell lines or lung cancer cell lines. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting of the extractable antigen from SW1116 showed a single band at approximately 45, 000 dalton formed by 125 I-labeled Mab A7. Treatment of SW1116 with sodium periodate, pronase and ficin resulted in the loss of antigenic activity. These data strongly suggest that the antigen recognized by Mab A7 is composed of glycoprotein. Competitive binding analysis to the surface of the colon cancer cell line using polyclonal anti-CEA and Mab A7 as well as immunoblotting analysis using monoclonal anti-CEA and Mab A7 suggested that the antigen recognized by Mab A7 was different from CEA. Moreover, this antigen was also found in surgical specimens of colorectal cancer patients and its molecular property was identical to the antigen extracted from SW1116.抄録全体を表示PDF形式でダウンロード (1838K) -
FUMIHITO TAGUCHI, JINICHI SUZUKI, MOTOYASU MURANAKA, NORMAN B. ANDERSO ...1989 年 157 巻 2 号 p. 95-106
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーTAGUCHI, F., SUZUKI, J., MURANAKA, M., ANDERSON, N.B. and WILLIAMS, R.B., Jr. Beta and Alpha Adrenergic Reactivity Elicitable Stress Study with Special Reference of Electrocardiographic T-Wave Amplitude. Tohoku J. Exp. Med., 1989, 157 (2), 95-106-Twenty-six healthy young Caucasian males were defined into high hostile (Hi-Ho) group and low hostile (Lo-Ho) group assessed by Cook-Madley's Hostility (Ho) scale. Mental arithmetic task (MA) and forehead cold stimulus task (FCS) were loaded to both Hi-Ho and Lo-Ho groups. Electrocardiographic T-wave amplitude (TWA), heart rate (HR) and coefficient of variance of 100 R-R intervals (CVR-R) were measured continuously during MA and FCS task periods. Greater TWA attenuation was found in Hi-Ho group (p< 0.05). Although no significant intergroup difference was represented in HR and CVR-R, HR increased significantly (p<0.01) in whole subjects and CVR-R was tend to be suppressed during MA period. In addition, comparison of these physiological responses were performed between Type-A and Type-B groups classified by Jenkins Activity Survey Form-T (JAS-T). There was no significant difference in reactivity of TWA, HR and CVR-R to both two tasks between high and low Type-A scored groups. Previous data suggested that the TWA reactivity in Hi-Ho subjects to cognitive stress showed similar pattern in Type-A individuals. However, autonomic nervous interaction could not be clarified in Hi-Ho subjects. The differentiation of method for assessment of behavioral pattern was also discussed.抄録全体を表示PDF形式でダウンロード (1221K) -
KEIJI TAMURA, HISASHI ASO, TAKEHIKO NAKAMURA, HIROMICHI HEMMI, NAKAO I ...1989 年 157 巻 2 号 p. 107-118
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーTAMURA, K., ASO, H., NAKAMURA, T., HEMMI, H. and ISHIDA, N. Evaluation of Recombinant Human Tumor Necrosis Factor by Scheduled Intratumoral Administration in Mice Bearing Transplantable Tumors. Tohoku J. Exp. Med., 1989, 157 (2), 107-118-The antitumor effect of recombinant human tumor necrosis factor (rTNF) was examined against Meth A fibrosarcoma in BALB/c mice and Sarcoma-180 in ddY mice. Significant hemorrhagic necrosis in tumor tissues occurred within 24hr when optimal rTNF (1, 000 to 5, 000 units per mouse) was injected intratumorally on day 5 after intradermal inoculation of 5×105 tumor cells. Complete tumor regression resulted when two repeated courses of administration a week, each for 3 consecutive days, were given. For this marked effect to occur, however, initial tumor weight should not be greater than 1g. When the initial tumor was greater than 1g the surgical removal of tumor tissues was conducted and followed by rTNF administration. This caused hemorrhagic necrosis and the regression was the case with smaller tumors. When the cured mice were rechallenged with same tumors, more than 60% of mice rejected the tumors in a specific manner. In spite of such demonstration of specific immunity, well-known immunological effector mechanisms such as augmentation of natural killer cell activity, activation of antibody dependent cellular cytotoxicity or induction of interferon activity by rTNF were not detected in normal and tumor-bearing mice, suggesting that the activation of immunoregulatory cells by TNF itself may not involve at least in an early stage of TNF treatment. These results suggest that rTNF is a potent therapeutic agent for a certain solid tumor when the protocol of administration is optimized.抄録全体を表示PDF形式でダウンロード (2149K) -
SHUICHI KATO, SHINOBU SATO, KEIJI TAKAHASHI1989 年 157 巻 2 号 p. 119-129
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーKATO, S., SATO, S. and TAKAHASHI, K. Almitrine Bismesylate Reduces Hypoxic Pulmonary Vasoconstriction in Isolated Rat Lungs. Tohoku J. Exp. Med., 1989, 157 (2), 119-129-The purpose of this study is to test how almitrine bismesylate (Alm) affects the function of pulmonary vasculature during normoxic ventilation, and whether low doses of Alm not causing detectable vasoconstriction during normoxic ventilation potentiate hypoxic pulmonary vasoconstriction (HPVC). Isolated Wistar male rat lungs were perfused with homologous blood at constant flow, and venous and ventilatory pressure. In the first experiment, after equilibration, dose-response curves to Alm (from 0 to 1000ng/ml, n=10) were measured under the ventilation with normoxic gas mixture (21% O2, 5% CO2, 74% N2). It was found that Alm causes a dose-dependent pulmonary vasoconstriction. In the second experiment, low doses of Alm (125ng/ml) or diluent of Alm (malic acid) was injected to the blood reservoier. This doses of Alm did not cause significant vasoconstriction during normoxic gas ventilation compaired with malic acid. After stabilization of pulmonary arterial pressure, the lungs were exposed to three cycles of normoxia (10min) and hypoxia (10min) through ventilation with gas containing 21% or 2% O2 and 5% CO2. It was observed that low doses of Alm significantly reduce HPVC (p<0.05) on the later periods of the first and the second hypoxic challenges. However, no significant difference was revealed among two groups in the third hypoxic challenge. Directly measured blood Alm concentration was significantly lower in the third challenge than in the first challenge. Responses to angiotensin II were not decreased by Alm. In conclusion, high doses of Alm constrict pulmonary vasculature dose-dependently, and low doses of the drug not causing vasoconstriction during normoxia reduce HPVC in rat.抄録全体を表示PDF形式でダウンロード (1075K) -
SHUJI TAKAGI, JUNICHI MINAKUCHI, HIROJI OKAWA, JUNICHI YATA1989 年 157 巻 2 号 p. 131-136
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーTAKAGI, S., MINAKUCHI, J., OKAWA, H. and YATA, J. Interferon-Induced Resistance of Tumor Target Cells against Lysis by Interleukin-2-Activated Killer Cells. Tohoku J. Exp. Med., 1989, 157 (2), 131-136-IFN-γ has been shown to decrease the susceptibility of target cells to NK cell-mediated cytotoxicity. In this report, the effect of IFN-γ on the sensitivity of target cells to killing by various human lymphocyte cytotoxic activities such as NK/K, IL-2-augmented NK/K cell activity, and IL-2-activated killer activity were studied. Although NK-sensitive K562 cells showed marked resistance to NK cell activity as previously reported, the resistance was overwhelmed by augmentation of NK activity with IL-2. IL-2-activated killer cell activity, which can lyse NK-resistant tumor cell lines upon culture in IL-2, showed decreased cytotoxicity against most of the IFN-γ-treated target cells tested. By contrast, no decrease of target cell sensitivity to K cells was observed, even though K cells were treated with IL-2. These findings suggest that as far as NK-resistant tumor cells are concerned, an IFN-dependent mechanism inhibits the Fc receptor-independent mediators of tumor surveillance, but not Fc receptor-dependent ones. This should be considered when planning adoptive immunotherapy of IL-2-activated killer cells for human malignancy.抄録全体を表示PDF形式でダウンロード (554K) -
TAKASHI YOKOTA, TOSHIO TAKAHASHI, TOSHIHARU YAMAGUCHI, KIYOSHI SAWAI, ...1989 年 157 巻 2 号 p. 137-144
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーYOKOTA, T., TAKAHASHI, T., YAMAGUCHI, T., SAWAI, K., HAGIWARA, A., SHIMOTSUMA, M., DOI, M., MASUKO, T. and HASHIMOTO, Y. Scirrhous Carcinoma Cell Invasion into the Stomach Wall Detected by Monoclonal Antibody S202: A Comparison between Immunoperoxidase and Hematoxylin-Eosin Stain. Tohoku J. Exp. Med., 1989, 157 (2), 137-144-We used anti-scirrhous carcinoma monoclonal antibody (MAb) S202, which reacted strongly with scirrhous gastric cancer, to evaluate the depth of cancer cell invasion into the stomach wall. Sixty-eight scirrhous gastric cancer specimens were stained with the avidin-biotin peroxidase complex method using MAb S202. There were some discrepancies between the histological evaluation of the immunoperoxidase (IP) and hematoxylin-eosin (HE) staining regarding the depth of tumor invasion. In two of the 14 HE staining cases in which carcinoma had extended as far as the proper muscular layer, IP staining revealed that the lesions had actually penetrated this layer. As well, in two of the 18 HE staining cases in which carcinoma cells had not penetrated as far as the serosa, IP staining revealed that they had, in fact, reached the serosal surface. The invasive carcinoma cells were more easily discerned using IP staining with MAb S202 rather than using histological staining with HE. Thus, MAb S202 may be useful for precise identification of carcinoma cells抄録全体を表示PDF形式でダウンロード (2571K) -
YOSHIAKI YAJIMA, JO SATOH, ICHIRO FUKUDA, TAKAO KIKUCHI, TAKAYOSHI TOY ...1989 年 157 巻 2 号 p. 145-151
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーYAJIMA, Y., SATOH, J., FUKUDA, I., KIKUCHI, T. and TOYOTA, T. Quantitative Assay of Lentinan in Human Blood with the Limulus Colorimetric Test. Tohoku J. Exp. Med., 1989, 157 (2), 145-151-A conventional limulus test detects not only endotoxin but also β (1→3) glucan. Therefore, using a quantitative limulus test (the limulus Colorimetric test) we studied the pharmacokinetics of lentinan, an antitumor β (1→3) glucan, in the blood of 10 healthy volunteers and three patients with advanced gastric cancer. The calibration curve of lentinan in the human plasma was linear in the range of 0 to 100ng/ml. When incubated with human plasma at 37°C in vitro, lentinan had the recovery of almost 100% as compared to the initial concentration even after 60-min incubation, indicating the stability of lentinan in human plasma. When 1mg of lentinan was intravenously administered over a 2hr period, lentinan concentration reached the maximum levels (50-80 ng/ml) at the end of the drip infusion and decreased gradually thereafter. In the near future, the more appropriate modes of lentinan administration will be determined by further investigation of its kinetics in the human body.抄録全体を表示PDF形式でダウンロード (524K) -
YUKITAKA FUKUSHIMA, MASAKO YANAGISAWA, TADASHI YASUDA, YASUSHI NAKAMOT ...1989 年 157 巻 2 号 p. 153-162
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーFUKUSHIMA, Y., YANAGISAWA, M., YASUDA, T., NAKAMOTO, Y. and MIURA, A.B. Erythropoietic Activity in Culture Media Conditioned by Rat Mesangial Cells. Tohoku J. Exp. Med., 1989, 157 (2), 153-162-Using a tissue culture technique we examined erythropoietin (EPO) producing cells in rat glomeruli. In 5 out of 6 independent glomerular cell cultures, EPO activity was found in the mesangial-cell proliferating phase, but not in the epithelial-cell proliferating phase. Therefore, mesangial cells seemed to be EPO producing cells.抄録全体を表示PDF形式でダウンロード (2518K) -
TAKEHIRO MITSUHASHI, HIDEKI KUWAHARA, YOSHIHIRO IKURA1989 年 157 巻 2 号 p. 163-176
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーMITSUHASHI, T., KUWAHARA, H. and IKURA, Y. Carrageenan-Induced Pulmonary Emphysema of Rabbit. Tohoku J. Exp. Med., 1989, 157 (2), 163-176-A transbronchial injection of 0.75% carrageenan in physiologic saline induced pneumonia followed by emphysema in the insulted lobe. In the stages of pneumonia, scattered infiltration of polymorphonuclear leukocytes was seen throughout the affected lobe within a few days of treatment; later this was replaced by the accumulation of carrageenan-laden macrophages, which lasted for one to two months. Enlargement of alveoli and alveolar ducts appeared 2 weeks to 2 months after the treatment, and pulmonary emphysema was observed at 4 months. The lobes that were not treated with carrageenan were normal in appearance during both the pneumonia and the emphysema. Morphometric analysis of the lung at 4 months showed decrease of the alveoli and/or alveolar ducts and enlargement of thier luminal spaces, also suggesting the development of emphysema. In contrast to various kinds of elastases that are known to produce emphysematous changes in animals, the elastolytic activity of carrageenan solution did not show any such effects, although in the homogenate of the lobes given carrageenan, a moderate but significant increase in the proteinase activities of alveolar macrophages are said to occur (Bowers et al. 1985). It was suggested that carrageenan-induced emphysema is a chronic disorder associated with both carrageenan toxicity and accumulated carrageenan-laden macrophages in the insulted lobes.抄録全体を表示PDF形式でダウンロード (5616K) -
JUNKO MAGARA, MASATO HAGA, WEISHI CHEN, KAZUO ENDOH, MASAHARU YAMAMOTO ...1989 年 157 巻 2 号 p. 177-182
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーMAGARA, J., HAGA, M., CHEN, W., ENDOH, K., YAMAMOTO, M., TSUTSUI, M., KATO, K. and AKAI, S. Rec-Assay of Human Bile for Mutagenicity and Co- Mutagenicity. Tohoku J. Exp. Med., 1989, 157 (2), 177-182-Spore rec-assay of human bile was conducted by the Bacillus subtilis test system to examine possible mutagenicity and co-mutagenicity. Of 26 samples examined, 8 (30.8%) showed mutagenic activity and 23 (88.5%) enhanced the mutagenic activity of mitomycin C.抄録全体を表示PDF形式でダウンロード (483K) -
YOSHIRO KOIWA, NOBUO HOSHI, TADASU OHYAMA, TAKEHIKO TAKAGI, JUN-ICHI K ...1989 年 157 巻 2 号 p. 183-184
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーKOIWA, Y., HOSHI, N., OHYAMA, T., TAKAGI, T., KIKUCHI, J., HONDA, H. and TAKISHIMA, T. The Response of Normal and Failing Heart to Externally Applied Vibration in the Canine Open Chest Preparation. Tohoku J. Exp. Med., 1989, 157 (2), 183-184-We examined the left ventricular functional response to externally applied vibration using four canine open chest preparations. A sinusoidal 30Hz vibration (2.7mm in amplitude) was applied to the ventricular epicardium at each level of propranolol-induced myocardial depression. External vibration in control conditions induced no significant change either in peak left ventricular pressure (LVP) or in stroke volume (SV). With propranolol, 0.1 and 0.3mg/kg, peak LVP and SV were depressed by the application of external vibration, even though there was no significant change of these values in the nonvibrating condition compared to control. We conclude that the ventricular response to vibration depends on the underlying myocardial viability.抄録全体を表示PDF形式でダウンロード (214K)
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