巻号一覧
157 巻 (1989)
- 4 号 p. 289-
- 3 号 p. 185-
- 2 号 p. 83-
- 1 号 p. 1-
157 巻, 3 号
選択された号の論文の12件中1～12を表示しています
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Expression Forms and Characteristics of Carr-Purcell-Meiboom-Gill Sequence Magnetic Resonance ImagesSUSUMU YAMADA, TAIJI MATSUZAWA, KENJI YAMADA, SHUICHI ONO, TAKASHI HIS ...1989 年 157 巻 3 号 p. 185-192
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーYAMADA, S., MATSUZAWA, T., YAMADA, K., ONO, S. and HISHINUMA, T. Expression Forms and Characteristics of Carr-Purcell-Meiboom-Gill Sequence Magnetic Resonance Images. Tohoku J. Exp. Med., 1989, 157 (3), 185-192 -We propose two expression forms of Carr-Purcell-Meiboom-Gill (CPMG) sequence magnetic resonance images using the imaging parameters of recovery time (Tr), echo interval (Te), echo number (n) from 1 to N (number of echoes). An echo image can be expressed as a form of CPMG (Tr, Texn) and the other image synthesized by summing consecutive echo images numbered from n1 to n2 (1≤n1< n2≤N) as a form of CPMG (Tr, Te×n1-n2). The CPMG (Tr, Te×n) images are weighted by T1, T2 or proton density and show the better morphological representation and contrast resolution of brain tumor lesion. The difference of signal intensities among the tissues is enhanced on the synthesized CPMG (Tr, Te×n1-n2) images with the increased signal-to-noise ratio. The calculated T2 images are also observed. These forms expound the scanning conditions and image characteristics and make easy to be compared with the spin echo images.抄録全体を表示PDF形式でダウンロード (4778K) -
ICHIRO NAKAZAWA, MASAMOTO IWAIZUMI1989 年 157 巻 3 号 p. 193-198
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーNAKAZAWA, I. and IWAIZUMI, M. A Role of the Cancer Cell Membrane Fluidity in the Cancer Metastases: An ESR Study. Tohoku J. Exp. Med., 1989, 157 (3), 193-198 - AH66F or Yoshida sarcoma (YS) cells were transplanted intraperitoneally into male Donryu rats. Cancer cells obtained from ascites were suspended in saline solution (107cells/ml) after washing. Then, 0.1ml of each suspension obtained from both strains was injected into the tail vein of 5 rats, respectively. Each metastatic nodule, 1mm or less in a diameter, thus obtained was then injected into the peritoneal cavity in which these metastatic cells come to free. After 10 days, cancer cells obtained from each ascites were suspended in phosphate buffered saline (Ca2+ and Mg2+ free, pH 7.2) after washing. Each suspension (107cells/ml) was violently vibrated with a definite amo unt of 5-doxylstearic acid and spin labeling of cancer cell membrane was done. Furthermore, each specimen thus obtained was subjected to the electron spin resonance (ESR) measurement and the order parameter was determined from the spectra. In both YS and AH66F strains, the cell membrane fluidity of the metastatic cancer cell was increased at each temperature measured from 5°C through 35°C. The results obtained here suggest that the change of the cell membrane fluidity of cancer cell is closely related with the cancer metastases.抄録全体を表示PDF形式でダウンロード (597K) -
SHIGETOSHI CHIBA, MIYOKO TSUKADA1989 年 157 巻 3 号 p. 199-204
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーCHIBA, S. and TSUKADA, M. Pharmacological Features of Vascular Responses of Isolated Rat Common Carotid Arteries to Vasoactive Substances Revealed by the Cannula Inserting Method. Tohoku J. Exp. Med., 1989, 157 (3), 199-204 -Using the cannula inserting method, we studied vascular responses of isolated rat common carotid arteries to 15 vasoactive substances. Intraluminal injections of phenylephrine, norepinephrine (NE), angiotensin II, 5-hydroxytryptamine (5-HT) and prostaglandin F2α (PGF2α) induced a strong vasoconstriction in such a way that a maximum increase in perfusion pressure amounted to approximately 50 mmHg. The order of their potencies were as follows: Phenylephrine ≥NE≥angiotensin II>PGF2α≥5-HT<<KCl. The selective alpha-2 agonists, xylazine and clonidine, induced no or a slight vasoconstriction. Tyramine, ATP and acetaldehyde induced only a slight constriction. Histamine, adenosine and acetylcholine (ACh) induced no effect. In preparations preconstricted by a high dose of NE, ACh and isoproterenol induced a vasodilation in a dose-related manner. It is concluded that the cannula inserting method is useful for investigating vascular responses in isolated and perfused rat vessels.抄録全体を表示PDF形式でダウンロード (609K) -
KUNIHIKO ITOH, TOSHIRO MAJIMA, KIYOTO EDO, MICHINAO MIZUGAKI, NAKAO IS ...1989 年 157 巻 3 号 p. 205-214
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーITOH, K., MAJIMA, T., EDO, K., MIZUGAKI, M. and ISHIDA, N. Suppressive Effect of 1-Methyladenosine on the Generation of Chemiluminescence by Mouse Peritoneal Macrophages Stimulated with Opsonized Zymosan. Tohoku J. Exp. Med., 1989, 157 (3), 205-214 - The preparation of an in vitro assay system for the immunosuppressive activities of modified nucleosides on macrophage (Mφ) functions is described. Briefly, Listeria-elicited mouse peritoneal Mφs (Lm-Mφs) were treatd with nucleosides in vitro for 18 hr at less than 1mM concentration and chemiluminescence (CL) was measured after stimulation with opsonized zymosan. To confirm the usefulness of this assay (in vitro test), the immunosuppressive activities of 1-methyladenosine (m1Ado) and adenosine (Ado) were determined by mouse Listeria infection (in vivo test), CL generation by Mφs obtained from the peritoneal cavity of nucleoside-treated mice (in vivo-in vitro test), and the in vitro test. The immunosuppressive activity of m1Ado detected by the in vitro test was confirmed by the in vivo and the in vivo-in vitro tests. As for Ado, no immunosuppression was detected by the in vivo and the in vivo-in vitro tests though a potent suppressive effect was detected by the in vitro test. The ineffectiveness of Ado can be explained by the in vivo conversion of Ado to an inactive form. Thus, the proposed in vitro test seems to be useful, with the provision that the known in vivo metabolism is taken into consideration.抄録全体を表示PDF形式でダウンロード (936K) -
Establishment of an Etoposide (VP-16)-Resistant Subline of THP-1 Human Monocytic Leukemia Cell LinesYASUO SAIJO, NOBUKO KUMANO, SHUJI SUZUKI, SADAHIRO KOINUMARU, SHUNICHI ...1989 年 157 巻 3 号 p. 215-219
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーSMJO, Y., KUMANO, N., SUZUKI, S., KOINUMARU, S., SUGAWARA, S., OIZUMI, K. and MOTOMIYA, M. Establishment of an Etoposide (VP-16)-Resistant Subline of THP-1 Human Monocytic Leukemia Cell Line. Tohoku J. Exp. Med., 1989, 157 (3), 215-219 - A VP-16-resistant subline was established from THP-1 human monocytic leukemia cells by subculturing in stepwise increasing concentrations of the drug. The resistant cells (THP-1/E) were capable of sustaining continuous growth in 1.0μg/ml VP-16. The 50% inhibition dose (IC50) was 1.4μg/ml or a 35-fold of the parent cells. A more than 18-fold IC50 was also obtained with teniposide (VM-26). However, no substantial cross-resistance was observed with 5 other antineoplastic drugs, IC50 values being limited below several times of THP-1.抄録全体を表示PDF形式でダウンロード (442K) -
YUTAKA IMAI, KEISHI ABE, SHUICHI SASAKI, NAOYOSHI MINAMI, MINORU NIHEI ...1989 年 157 巻 3 号 p. 221-227
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーIMAI, Y., ABE, K., SASAKI, S., MINAMI, N., NIHEI, M., MUNAKATA, M., SEKINO, H. arid YOSHINAGA, K. Modulation of Cardiovascular Depressant Action of Clonidine by Pentobarbital Anesthesia in Rats. Tohoku J. Exp. Med., 1989, 157 (3), 221-227 - The influence of pentobarbital anesthesia (5mg/100g, i.p.) on the cardiovascular depressor effect of clonidine administered intracerebroventricularly (i.c.v.) and intravenously (i.v.) was examined in rats. In conscious rats the hypotensive potency of i.v. clonidine [effective dose of clonidine which induced a decrease in blood pressure of 15mmHg; ED15=7 (μg/kg)] is greater than that of i.c.v. clonidine (ED15=24.3), while in anesthetized rats that of i.c.v. clonidine (ED15-0.7μg/kg) was greater than that of i.v. clonidine (ED15= 3.5μg/kg). Pentobarbital anesthesia potentiated the hypotensive potency of i.c.v. clonidine in conscious rats by 50 times, while it potentiated that of i.v. clonidine only by 2 times. The bradycardic potency of i.v. and i.c.v, clonidine was also potentiated by pentobarbital anesthesia. These pieces of evidence suggest that in conscious rat, the hypotensive action of i.c.v. clonidine is opposed by a centrally mediated hypertensive effect of the drug and that pentobarbital anesthesia suppresses the clonidine sensitive pressor center and so potentiates the hypotensive effect of i.c.v. clonidine抄録全体を表示PDF形式でダウンロード (644K) -
KAZUHIRO KIDO, YASUMOTO MAGARA, TOHRU FURUICHI, MASAYUKI IKEDA1989 年 157 巻 3 号 p. 229-239
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーKIDO, K., MAGARA, Y., FURUICHI, T. and IKEDA, M. Statistical Approach towards Point Sources of Groundwater Pollution with Tetrachloroethylene: A Field Study. Tohoku J. Exp. Med., 1989, 157 (3), 229-239-Tetrachloroethylene contamination of well water occurred in a primarily residential area. To search for point source(s) of tetrachloroethylene contamination, 91 water samples were collected on three separate occasions from 41 shallow wells scattered in the areas. Three methods of groundwater level analysis (limited to 30 wells), cluster analysis of water quality indicators and contour drawing of tetrachloroethylene concentrations were applied. The former two analyses showed that the pollution took place in aquifers of two terraces out of the three in the polluted area. The contour mapping demonstrated the presence of three spots of suspected pollution sources as the estimated points of highest tetrachloroethylene concentrations. The available information suggested the existence of a facility with possible use of tetrachloroethylene in the past.抄録全体を表示PDF形式でダウンロード (999K) -
SHINSUKE YOSHIMURA, KIYOSHI IMAI, YUZO HAYASHI, KOROKU HASHIMOTO1989 年 157 巻 3 号 p. 241-249
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーYOSHIMURA, S., IMAI, K., HAYASHI, Y. and HASHIMOTO, K. Role of Renin-Angiotensin System in the Pathogenesis of Spontaneous Myocardial Fibrosis in Sprague-Dawley Rats: Effect of Long-Term Administration of Captopril. Tohoku J. Exp. Med., 1989, 157 (3), 241-249-The role of endogenous rennin-angiotensin system in the pathogenesis of spontaneous myocardial fibrosis in male and female Sprague-Dawley rats was examined by long-term inhibition of endogenous angiotensin II production. For this purpose captopril, angiotensin I converting enzyme inhibitor (ACEI), was given at 30 or 100mg/kg/day mixed with commercial powdered standard diet (CE-2, Japan Clea Co., Tokyo) for 52 weeks. Myocardial lesions consisting of focal fibrosis and minute necrotic foci of muscle cells intermingled with monocytic infiltration appeared preferentially in the subendocardial areas of the left ventricle. The incidence of myocardial fibrosis was very low in female rats, but in male rats, the incidence and severity of the lesion was significantly reduced in the captopril-treated groups than in the control group. These findings suggest that endogenous angiotensin II acts as a facilitatory effect on the pathogenesis of spontaneous myocardial lesion which can be effectively reduced by long-term administration of nontoxic dose of ACEI. The authors speculate that ACEI may have a therapeutic use in clinical cases of idiopathic myocarditis.抄録全体を表示PDF形式でダウンロード (2260K) -
NARIHIDE GOSEKI, MITSUO ENDO, TOKIO ONODERA, GORO KOSAKI1989 年 157 巻 3 号 p. 251-260
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーGOSEKI, N., ENDO, M., ONODERA, T. and KOSAKI, G. Influence of L-Methionine-Deprived Total Parenteral Nutrition on the Tumor Tissue and Plasma Amino Acids Fraction and the Host Metabolism: Experimental Study with Sato Lung Carcinoma-Bearing Rats. Tohoku J. Exp. Med., 1989, 157 (3), 251-260-Creation of an amino acid imbalance, particularly curtailment of L-methionine, at the tumor cell level is thought to have a favorable effect on the inhibition of tumor growth. In the present study, we examined the influence of a specially-formulated amino acid mixture, avoid of sulfur-containing amino acids (L-methionine and L-cysteine), on the growth and amino acid fraction of Sato lung carcinoma (SLC) and the host metabolism in SLC-bearing rats. The rats were treated by total parenteral nutrition containing the above amino acid mixture, plus other nutrients (methionine-deprived TPN) for 10 days. Tumor growth began to decrease 4 days after the start of this treatment and the size was significantly less at the end of the treatment than in rats receiving conventional TPN with general purpose Vuj-N type amino acid solution as a protein source. The tumor-to-carcass weight ratio also showed a similar trend. In biochemistry, the albumin level and albumin-to- globulin ratio were significantly lower than in the rats receiving conventional TPN but other parameters such as total protein, glucose, GOT and GPT were not affected by the treatment. In the amino acid fraction of the tumor tissue extraction, both L-methionine and L-tyrosine were decreased and L-serine was increased significantly compared with the control group.抄録全体を表示PDF形式でダウンロード (773K) -
YUICHIRO TANAKA, SHIGETOSHI CHIBA1989 年 157 巻 3 号 p. 261-267
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーTANAKA, Y. and CHIBA, S. A Modified Cannula Inserting Method for Measuring Vascular Responsiveness of the Common Carotid Artery of the Dog. Tohoku J. Exp. Med., 1989, 157 (3), 261-267- A modified cannula inserting method was developed to investigate effects of either intra- or extraluminal application of vasoactive drugs on the isolated vessel which was cannulated and suspended in an organ bath and perfused under a constant flow rate with modified Krebs solution. By inserting a newly developed stainless steel cannula into the vessel, the space between the internal surface of the vessel and the cannula was filled with perfusate and perfused by a pump. Drugs were administered intraluminally through a cannula or extraluminally in the bath. The perfusate after passing through the inside of the vessel was introduced to the tubing and then drained to the outside. Although a lot of methods have been used to observe contractile responses of isolated vessels, this method may be superior to them as follows: (1) drugs act selectively on the inner or outer surface of the vessel; (2) vascular responses to drugs, not only constriction, but also dilatation, are readily observed in a relatively larger artery (1-3mm I.D.); 3) the vascular reactivity is kept in a stable condition over 7-8hr at 37°C because reproducible responses were consistently observed. We used this preparation to examine the constrictive and dilatory responses of the canine common carotid artery to extraluminally administered oxyhemoglobin, and intraluminally injected 5-hydroxytryptamine, nor-epinephrine and ACh.抄録全体を表示PDF形式でダウンロード (613K) -
HIROSHI SHIMIZU, KENJI HOSODA, MASAHIKO MIZUMOTO, YOSHIO KUROKI, HIDEK ...1989 年 157 巻 3 号 p. 269-278
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーSHIMIZU, H., HOSODA, K., MIZUMOTO, M., KUROKI, Y., SATO, H., KATAOKA, K., HAGISAWA, M., FUJIMOTO, S. and AKINO, T. Improved Immunoassay for the Determination of Surfactant Protein A (SP-A) in Human Amniotic Fluid. Tohoku J. Exp. Med., 1989, 157 (3), 269-278- A simple, improved immunoassay for the determination of human surfactant protein A (SP-A) in human amniotic fluid was developed. The immunoreaction with a monoclonal antibody PC6-immobilized plastic bead, peroxidase-labeled monoclonal antibody PE10 and amniotic fluid sample diluting in the buffer containing 0.6% sodium dodecyl sulfate/2% Triton X-100, was carried out simultaneously at 45°C for 30min in test tubes. After washing the bead with 2% skimmilk in phosphate buffered saline containing 1% Triton X-100, the peroxidase reaction was developed by adding the substrate reagent and the absorbance was measured. The amniotic fluid obtained at full term was used as a standard instead of purified SP-A, because of the stability of the antigenicity. This immunoassay method was used to measure SP-A in 69 samples of amniotic fluid from 22 to 41 weeks of gestation. The result indicated that the SP-A values obtained by the present immunoassay can be used for predicting the fetal lung maturity. This simplified monoclonal immunoassay completed the measurement within 1hr, and so it could be used routinely in clinical laboratory.抄録全体を表示PDF形式でダウンロード (921K) -
MASAHIRO KOHZUKI, KEISHI ABE, MINORU YASUJIMA, YUTAKA KASAI, MASAYUKI ...1989 年 157 巻 3 号 p. 279-288
発行日: 1989年
公開日: 2006/08/31
ジャーナル フリーKOHZUKI, M., ABE, K., YASUJIMA, M., KASAI, Y., KANAZAWA, M., SATO, M., HIWATARI, M., OMATA, K., KUDO, K., TAKEUCHI, K. and YOSHINAGA, K. Acute and Chronic Effects of Synthetic Atrial Natriuretic Factor on Blood Pressure and Sodium Excretion in Spontaneously Hypertensive Rats. Tohoku J. Exp. Med., 1989, 157 (3), 279-288- To assess possible roles of atrial natriuretic factor (ANF) in the regulation of blood pressure in spontaneously hypertensive rats (SHR), we performed two series of experiments. First, we studied acute hypotensive, natriuretic and diuretic effects of ANF in pentobarbital-anesthetized SHR and age-matched Wistar-Kyoto rats (WKY). A synthetic ANF of 25 amino acid residues was intravenously administered as a bolus at doses of 0.1, 1.0, 2.5 and 5.0 μg/kg. In SHR group, a significant decrease in mean arterial pressure (MAP) was observed at a dose of 1.0μg/kg, and the decrease was dose-dependent. On the other hand, in WKY group, the hypotensive effect of ANF was not observed until a dose of 5.0μg/kg. The diuretic and natriuretic effects of ANF were observed at a dose of 2.5μg/kg in SHR and 5.0μg/kg in WKY, respectively. Second, we also studied chronic effect of ANF on the development of hypertension in 6- week-old SHR. The SHRs, on regular diet or given 1% NaCl solution for drinking, were continuously infused into the jugular vein by osmotic minipumps with ANF (15, 75 and 150μg/kg/day) or vehicle (physiological saline) as controls for up to 14 days. ANF at a dose of 150μg/kg/day attenuated transiently the development of hypertension in the sodium-loaded SHR. However, the blood pressure returned to control levels by day 5. ANF at doses of 15 and 75μg/kg/day did not affect the development of hypertension. In SHR on regular diet, ANF at a dose of 150μg/kg/day did not affect the development of hypertension. In addition, ANF did not induce any significant changes in urine volume, fluid intake, and urinary excretion of sodium and potassium in SHR, whether they were sodium-loaded or not, when compared to those in vehicle-infused SHR. These results indicate that there may be a significant difference in the sensitivity to diuretic, natriuretic and hypotensive actions of ANF between SHR and WKY. Moreover, it is suggested that ANF may play significant roles by its vascular effect at the early stage of development of hypertension in sodium-loaded SHR.抄録全体を表示PDF形式でダウンロード (1048K)
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