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Volume 157 (1989)
- Issue 4 Pages 289-
- Issue 3 Pages 185-
- Issue 2 Pages 83-
- Issue 1 Pages 1-
Volume 157, Issue 4
Displaying 1-10 of 10 articles from this issue
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JIRO HIROSUMI, YASUYOSHI OUCHI, MASAE WATANABE, JUN KUSUNOKI, TETSURO ...1989 Volume 157 Issue 4 Pages 289-300
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSHIROSUMI, J., OUCHI, Y., WATANABE, M., KUSUNOKI, J., NAKAMURA, T. and ORIMO, H. Effects of Growth Factors on Cytosolic Free Calcium Concentration and DNA Synthesis in Cultured Rat Aortic Smooth Muscle Cells. Tohoku J. Exp. Med., 1989, 157 (4), 289-300- Proliferation of vascular smooth muscle cells (SMCs) has been considered to be an important process in the development of atherosclerosis. This study was conducted to investigate the role of cytosolic free calcium in DNA synthesis of SMCs stimulated by growth factors. Platelet-derived growth factor (PDGF), epidermal growth factor (EGF) and somatomedin-C (Sm-C) increased [3H]thymidine incorporation, an index of DNA synthesis, and cell number of rat aortic SMCs after 36hr of incubation. Cytosolic free calcium concentration ([Ca++]i) in quiescent SMCs, measured by using quip 2, was 178+ 18nM (n=15). Both PDGF and EGF provoked a rapid and transient rise in [Ca++]i, while Sm-C did not alter [Ca++]i. Nifedipine (3x10- 6M) suppressedthe rise in [Ca++]i provoked by PDGF and EGF. On the other hand, nifedipine suppressed the enhancement of DNA synthesis provoked by EGF, but did not suppress those by PDGF and Sm-C. These results suggest that the transient rise in [Ca++]i plays an important role in the proliferation of SMCs stimulated by EGF, while the rise in [Ca++]i is not involved in the mechanism of proliferation of SMCs provoked by Sm-C. The role of cytosolic free calcium in the proliferation of SMCs provoked by PDGF was not definitive.View full abstractDownload PDF (1195K) -
MASAHIRO KOHZUKI, KEISHI ABE, MINORU YASUJIMA, YUTAKA KASAI, MASAO HIW ...1989 Volume 157 Issue 4 Pages 301-311
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOHZUKI, M., ABE, K., YASUJIMA, M., KASAI, Y., HIWATARI, M., KANAZAWA, M., SATO, M., OMATA, K., KUDO, K., TAKEUCHI, K. and YOSHINAGA, K. Blood Pressure and Renal Responses to Synthetic Rat Atrial Natriuretic Factor in Deoxycorticosterone Acetate-Salt Hypertension. Tohoku J. Exp. Med., 1989, 157 (4), 301-311- To assess possible roles of atrial natriuretic factor (ANF) in the regulation of blood pressure in deoxycorticosterone acetate (DOCA)-salt hypertensive rats, we performed two series of experiments. First, we studied acute hypotensive, and natriuretic and diuretic effects of ANF in pentobarbital-anesthetized DOCA-salt hypertensive rats and age-matched controls. A synthetic rat ANF was intravenously administered as a bolus at doses of 0.5, 2.5 and 5.0μg/kg. In DOCA-salt rats, a significant decrease in mean arterial pressure was observed at a dose of 5.0μg/kg, whereas at a dose of 2.5μg/kg in control rats. On the other hand, the diuretic and natriuretic effects of ANF were observed at a dose of 2.5μg/kg in DOCA-salt rats and 5.0μg/kg in control rats. Second, we examined c hronic effect of ANF on the development of hypertension in DOCA-salt rats. The DOCA-salt rats, given 1% NaCl solution for drinking, were continuously infused with ANF (15, 75 and 150μg/kg/day) or vehicle (physiological saline) into the jugular vein by osmotic minipumps for up to 14 days. In DOCA-salt treated rats, ANF at doses of 75 and 150μg/kg/day attenuated significantly the development of hypertension, although ANF at a dose of 15μg/kg/day did not. The hypotensive effect of ANF was sustained throughout the experimental period and the effect of ANF at a dose of 150μg/kg/day was more prominent than that of this peptide at a dose of 75μg/kg/day. ANF did not induce any significant changes in urine volume, fluid intake and urinary excretion of sodium and potassium in DOCA-salt rats when compared to those in vehicle-infused DOCA-salt rats. These results indicate that DOCA-salt rats are more sensitive to ANF in diuretic and natriuretic effects, and less sensitive to ANF in hypotensive effect compared to control rats. Moreover, it is suggested that ANF can affect the regulation of blood pressure by its vascular effect in the development of hypertension in DOCA-salt rats.View full abstractDownload PDF (1163K) -
TAIRA OKAMOTO, SHINICHI OIKAWA, TAKAYOSHI TOYOTA1989 Volume 157 Issue 4 Pages 313-324
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSOKAMOTO, T., OIKAWA, S. and TOYOTA, T. Effect of Eye-Derived Growth Factors from Rabbit Retina on Proliferation of Endothelial Cell from Marginal Vessels. Tohoku J. Exp. Med., 1989, 157 (4), 313-324- The biological activity of eye-derived growth factor(s) (EDGF) was studied in endothelial cells and other cell types. The endothelial cells of rabbit ears were aseptically collected from marginal vessels in a short time without contamination with other cell types. The cells were cultured in 10% FBS-MEM that possessed Factor VIII antigen and Weibel-Palade bodies, and showed monolayer cobble-stone formation. The retinal extracts from rabbits promoted not only endothelial cells but also fibroblasts and smooth muscle cells. Partially purified EDGF was obtained from retinal extracts by means of acetone precipitation and successive chromatographic separation with Heparin Sepharose and Sephadex G-200. The molecular weight was estimated at more than 150kDa by gel filtration. The partially purified EDGF promoted proliferation of endothelial cells from rabbit ear marginal promoted vessels and bovine aorta, but did not affect smooth muscle cells, skin fibroblasts and Swiss albino 3T3 cells. Thus rabbit EDGF might be cell specificity in stimulating cell proliferation.View full abstractDownload PDF (1109K) -
NAOKO KONO, YASUNI NAKANUMA1989 Volume 157 Issue 4 Pages 325-335
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKONO, N. and NAKANUMA, Y. Localization of Actin and Cytokeratin in Hepatocytes and Biliary Epithelial Cells in Normal Human Livers Using a Low Temperature Resin and Post-Embedding Immunohistochemistry. Tohoku J. Exp. Med., 1989, 157 (4), 325-335- Using a low temperature resin Lowicryl K4M and post-embedding immunogold labeling, localization of actin and cytokeratin was examined in hepatocytes and biliary epithelial cells in normal human livers. Preservation of the liver tissues was satisfactory at the electron microscopic level. In immunolabeling of actin, gold particles were mainly found on microvilli and around the junctional complexes and at cell borders in the hepatocytes as well as biliary epithelial cells. In immunolabeling of cytokeratin, the gold particles were rather preferentially found in the perinuclear cytoplasm and around the junctional complexes in the biliary epithelial cells and hepatocytes. These particles were mainly found to be located on the intermediate filaments at higher magnification, although a considerable number of intermediate filaments were not labeled in the hepatocytes and biliary epithelial cells. Double staining method using the gold particles of different sizes clearly confirmed the abovementioned distribution of actin and cytokeratin in the same cells. From these observations, it was suggested that post-embedding immunoelectron microscopy using Lowicryl K4M is a useful tool for analysis of cytoskeletal organization in the liver tissue.View full abstractDownload PDF (4955K) -
YOSHIKAZU SATO, HARUO NAKATSUKA, TAKAO WATANABE, SHIGERU HISAMICHI, HI ...1989 Volume 157 Issue 4 Pages 337-343
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSATO, Y., NAKATSUKA, H., WATANABE, T., HISAMICHI, S., SHIMIZU, H., FUJISAKU, S., ICHINOWATARI, Y., IDA, Y., SUDA, S., KATO, K. and IKEDA, M. Possible Contribution of Green Tea Drinking Habits to the Prevention of Stroke. Tohoku J. Exp. Med., 1989, 157 (4), 337-343- Among 5910 nondrinking and nonsmoking women (of≥40 years of age) in a prefectural city of Sendai, and two villages of Tajiri and Wakuya in Miyagi prefecture, Japan, medical history of stroke was less frequently observed among those who took more green tea in daily life. No relation with tea drinking was observed for hypertension history. The uneven distribution of stroke history was detectable even after the effects of age, location of residence, and high salt intake were ruled out. The incidence of stroke and cerebral hemorrhage during a 4-year follow-up of the study population was twice or more times higher in those who took less green tea (<5 cups a day) than in those who took more (≥ cups daily).View full abstractDownload PDF (681K) -
NSIALA MBAKI, NAOTO RIKITOMI, MORITOSHI AKIYAMA, KEIZO MATSUMOTO1989 Volume 157 Issue 4 Pages 345-354
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMBAKI, N., RIKITOMI, N., AKIYAMA, M. and MATSUMOTO, K. In Vitro Adherence of Streptococcus pneumoniae to Oropharyngeal Cells: Enhanced Activity and Colonization of the Upper Respiratory Tract in Patients with Recurrent Respiratory Infections. Tohoku J. Exp. Med., 1989, 157 (4), 345-354- In vitro adherence of Streptococcus pneumoniae (S. pneumoniae) to oropharyngeal cells was assessed in 3 age matched groups of 29 subjects. The first group included patients with chronic pulmonary disease and recurrent respiratory infections due to S. pneumoniae or a recent respiratory infection caused by this organism. Patients of the second group has similar underlying pulmonary disease to the first group, but they had had no S. pneumoniae respiratory infection for at least the past 3 years. Healthy subjects or patients without underlying pulmonary disease constituted the third group. The mean adherence of S. pneumoniae to oropharyngeal cells was 10.6±4.7 bacteria (bact.)/cell in the first group, 3.6±2.8 bact./cell in the second group and 1.9±2.1 bact./cell in the third group. A significant difference was found (p<0.01) when the mean of the first group was compared to those of the second and third groups, whereas there was no significant difference between the means of the second and third groups. In addition, a survey of bacterial colonizationof the upper respiratory tract was conducted in all the subjects included in this study. Over 6 months, 5 of the 8 patients in the first group had throat cultures (5 cases) and nasal cultures (3 cases) positive for S. pneumoniae, while no one in the second or third group was a carrier of this bacterium in the upper respiratory tract during the same period. Patients with recurrent or recent respiratory infections caused by S. pneumoniae are characterised by an in vitro enhanced ability of this organism to adhere to their oropharyngeal cells. In vivo, this phenomenon is reflected by an increased susceptibility of the upper respiratory tract to colonization by S. pneumoniae.View full abstractDownload PDF (3209K) -
TATSUO KOHRIYAMA, MASARU KURIYAMA, RYOICHI HIWATARI, MITSUHIRO OSAME, ...1989 Volume 157 Issue 4 Pages 355-363
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOHRIYAMA, T., KURIYAMA, M., HIWATARI, R., OSAME, M. and IGATA, A. Partial Characterization of Leucocyte α-Glucosidase in Late Onset Glycogenosis Type II. Tohoku J: Exp. Med., 1989, 157 (4), 355-363- We describe partial characterization and properties of leucocyte α-glucosidase from a patient with clinical features intermediate of juvenile and adult onset forms of glycogenosis type II. Acid and neutral α-glucosidase activities toward 4-methylumbelliferyl glucopyranoside as substrate were studied in total leucocytes, and separately in lymphocytes and granulocytes. Lymphocytes, which showed markedly reduced activities of acid α-glucosidase in the patient, are the most reliable peripheral blood cells for the diagnosis of glycogenosis type II. Moreover, the ratio of acid/neutral α-glucosidase activities, especially in lymphocytes, is a useful parameter for the diagnosis. In lymphocytes, the Km values of both acid and neutral α-glucosidases were essentially the same between the patient and normal controls; the Vmax value of acid α-glucosidase from the patient was markedly reduced, and the Vmax value of neutral α-glucosidase from the patient was reduced by 36% as compared with that from normal controls. Heat-inactivation experiments revealed that acid α-glucosidase activities of lymphocytes were relatively heatstable, while both acid and neutral α-glucosidases of granulocytes were heat-labile. No differences in these properties, however, could be detected between the patient and normal controls.View full abstractDownload PDF (895K) -
YOICHI SUGITA, HITOSHI SUZUKI, KYOJI TASAKI1989 Volume 157 Issue 4 Pages 365-372
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSSUGITA, Y., SUZUKI, H. and TASAKI, K. Human Rods are Acting in the Light and Cones are Inhibited in the Dark. Tohoku J. Exp. Med., 1989, 157 (4), 365-372 -The light- and color detection thresholds were measured at three different states of retinal adaptation, i.e., light- and dark adapted states and the intermediate state which was obtained just before the Kohlrausch kink in the course of dark adaptation. At 20° from fixation in the nasal visual field, the wavelength function was examined. The color threshold was higher in the dark adapted state than in the state just before the kink, and the amount of increase was more prominent when the wavelength of the stimulus light was closer to the peak wavelength of rod sensitivity. It was concluded that the rod activation interferes with the cone sensitivity, and that the interference effect becomes stronger as a function of the responsiveness of rods. On the other hand, the spectral sensitivity curve determined in the light adapted state could be divided into two parts. The curve for shorter wavelength than 500nm was well fitted by the scotopic luminosity curve, suggesting that the light threshold for shorter wavelength is determined by the rod system even in the light adapted state.View full abstractDownload PDF (652K) -
TOYOHIRO TERASAKI1989 Volume 157 Issue 4 Pages 373-379
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTERASAKI, T. Effects of Autonomic Drugs on Intraluminal Pressure and Excretion of Rat Seminal Vesicles In Vivo. Tohoku J. Exp. Med., 1989, 157 (4), 373-379- The effects of intravenously administered autonomic drugs on the Intraluminal pressure and the excretion volume of the seminal vesicles in rats were studied. Both α1-adrenergic and cholinergic stimulants produced a rapid increase in pressure and fluid excretion. α1-Adrenergic stimulants elicited a sharp and single increase in pressure, while cholinergic stimulants caused a sharp increase with multiple peaks.View full abstractDownload PDF (671K) -
HARUO NAKATSUKA, MIYUKI KASAHARA, TAKAO WATANABE, MASAYUKI IKEDA1989 Volume 157 Issue 4 Pages 381-392
Published: 1989
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSNAKATSUKA, H., KASAHARA, M., WATANABE, T. and IKEDA, M. Continuity of Leanness/Obesity from Childhood to Adolescence. Tohoku J. Exp. Med., 1989, 157 (4), 381-392- Continuity and changes in leanness/obesity were studied following growth of the same individuals born in 1966 to 1968. Data on height and weight at ages 11, 14 and 18 were available for 770 boys and 1070 girls, and Rohrer's and Quetelet's indices were calculated from the data. In both indices, there were significant correlations between the values of the same individuals at age 11 and at age 14, and also at age 11 and at age 18. The subjects were divided at each age into 10 ranks of equal size so that those in Rank 1 (lean) and Rank 10 (obese) had the lowest and the highest index, respectively. The rate of those who were in Rank 1 or Rank 10 at age 11 but in medium ranks (Ranks 4-7) at age 18 was about 10 (lean) or 22% (obese) among boys, and 17 (lean) or 15% (obese) among girls when based on Rohrer's index. The analysis with Quetelet's index gave similar results. Children in the medium ranks also shifted into other ranks as they grew. Weight was more powerful than height as the determinant of the shifts from lean obese to medium and also from medium to lean/obese ranks.View full abstractDownload PDF (1196K)
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