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Volume 161 (1990)
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- Issue 3 Pages 159-
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Volume 161, Issue 3
Displaying 1-11 of 11 articles from this issue
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HIDEAKI TAKEMATSU, YASUKAZU OHMOTO, HACHIRO TAGAMI1990 Volume 161 Issue 3 Pages 159-169
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKEMATSU, H., OHMOTO, Y. and TAGAMI, H. Decreased Levels of IL-1α and β in Psoriatic Lesional Skin. Tohoku J. Exp. Med., 1990, 161 (3), 159-169- Interleukin 1 (IL-1), which mediates a wide range of biological activities, is thought to play an important role in many inflammatory and immunologic diseases. Normal human epidermal keratinocytes constitutively produce IL-1. Based on our previous data indicating decreased IL-1 activity in psoriatic scale extracts, in the present study, we measured immunoreactive IL-1α and β levels in the suction blister fluids as well as in the psoriatic scale extracts using enzyme immunoassay for IL-1α and β. The results showed that although similarly low levels of IL-1α were detectable in the suction blister fluid from normal and psoriatic Lesional skin, and that no IL-1β was found in most of the blister fluids, indicating that IL-1α is major IL-1 species produced by human skin. As compared to those in the blister fluids, IL-1α levels in the horny tissue extracts were found to be much higher, and they were significantly higher in the orthokeratotic stratum corneum extracts than in the psoriatic scale extracts. However, gel filtration of the orthokeratotic horny tissue extracts demonstrated that constituents for immunoreactive IL-1α and β were quite variable depending upon the source of the horny tissues. The present study has confirmed that IL-1 levels in the psoriatic scale extracts are decreased when compared with those in the orthokeratotic horny tissue possibly due to an increased epidermal proliferation activity associated with its high turnover rate. The role of IL-1 psoriatic lesions remains unknown.View full abstractDownload PDF (1113K) -
JUN-ICHI KADOTA1990 Volume 161 Issue 3 Pages 171-183
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKADOTA, J. Detection of Three Sialylated Tumor-Associated Antigens in Malignant Serous Effusions. Tohoku J. Exp. Med., 1990, 161 (3), 171-183- One hundred thirty-seven fluid samples from patients with malignant diseases, including 58 with lung cancer and 36 with gastric carcinoma, were screened for three tumor-associated carbohydrate antigens. The antigens tested were sialylated Lewis× (SLEX) antigen, sialylated SSEA-1 (SLX) antigen, and sialylatedBATU Lewisa (CA19-9) antigen. Thirty-four fluid samples from patients with benign diseases including 20 with tuberculous pleurisy were also tested for these three antigens as a control. SLEX antigen had the highest positivity rate among the three antigens both in the pleural effusions of patients with pulmonary adenocarcinoma and for all types of lung cancer (64.1% and 46.6%, respectively). In cancers of digestive system, the percentage of SLEX positivity was almost same as that for CA19-9, and higher than that for SLX. A combination assay using the three antigens resulted in an increased rate of detection of tumor-associated antigens (68% of 58 lung cancers including 83% of 39 adenocarcinomas, as well as 77% of 63 digestive system cancers including 84% of 36 gastric carcinomas). Gel filtration of pleural effusions or bile using sephacryl S-1000 showed that these three antigens were eluted in the void volume, indicating a molecular weight of >2×106. Thus, all 3 antigens possibly exist on carrier protein with a high molecular weight. These observations suggest the potential application of these antigens to distinguish between benign and malignant fluid, and to monitor the effects of the treatment of various types of cancer.View full abstractDownload PDF (1238K) -
KOICHI TABAYASHI, PETER P. MCKEOWN, MASAKI MIYAMOTO, ANDREW E. LUEDTKE ...1990 Volume 161 Issue 3 Pages 185-197
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTABAYASHI, K., MCKEOWN, P.P., MIYAMOTO, M., LUEDTKE, A.E., THOMAS, R., BREAZEALE, D.G., MISBACH, G.A., ALLEN, M. and IVEY, T.D. Myocardial Preservation: A Comparison of Oxygenated Crystalloid and Blood Cardioplegia. Tohoku J. Exp. Med., 1990, 161 (3), 185-197-The purpose of this experiment was to compare myocardial protective effect after global ischemia using oxygenatedcrystalloid (CCcO2) and an oxygenated blood (BCcO2) cardioplegic solutions. Post-ischemic ventricular performance was studied in 2 equal (n=7) groups of dogs subjected to 120min of global ischemia induced at average myocardial temperatures of 8°C in the CCcO2 group and 18°C in the BCcO2 group. Left ventricular (LV) function included analysis of LV systolic function (global and regional function), LV diastolic function (chamber and myocardial stiffness) and LV relaxation was measured by sonomicrometry and Millar micrometers. Data were processed with a Dec PDP-11/23 computer. In vitro oxygen content (Vol%) measured 3.2±1.0 (CCcO2) and 9.5±0.3 (BCcO2). Percent recoveries of LV global function (LVSP, loop area, % shortening, LV dp/dt, mean VCF and E max) in the CCcO2 group were approximately the same as those in the BCcO2 group. There were no significant differences in LV regional function (loop area and % shortening) after ischemia between the two groups. The chamber and myocardial stiffness after ischemia in the CCcO2 group were almost the same as the baseline values. Values in the BCcO2 group were reduced significantly compared to the baseline level. There were significant differences in post-ischemic chamber and myocardial stiffness between the two groups. Post-ischemic maximum negative LV dp/dt in both groups decreased significantly compared to the baseline values. However, the time constant and diastolic interval after ischemia in both groups were approximately the same as the baseline values. We conclude that there were no significant differences in myocardial protective effect between the CCcO2 and BCcO2 groups, and both methods preserved the ischemic myocardium well.View full abstractDownload PDF (1375K) -
KAZUYA KITAMURA, TOSHIO TAKAHASHI, AKINORI NOGUCHI, KEN-ICHIRO TAKASHI ...1990 Volume 161 Issue 3 Pages 199-207
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKITAMURA, K., TAKAHASHI, T., NOGUCHI, A., TAKASHINA, K., TSURUMI, H. and YAMAGUCHI, T. Binding, Internalization and the Cytotoxicity of Monoclonal Antibody A7-Neocarzinostatin Conjugates (A7-NCS) in Target Cells. Tohoku J. Exp. Med., 1990, 161 (3), 199-207-To study the mechanism of action of the monoclonal antibody A7-neocarzinostatin conjugates (A7-NCS), the internalization of the antibody and its conjugate into target cells was examined. The incubation of radiolabeled A7 and A7-NCS with target cells revealed that both were taken up by target cells in a time dependent fashion. The immunocytochemical study using anti-NCS also revealed the intracellular localization of the conjugates. The cytotoxicity of the conjugate was markedly reduced when the binding sites were occupied by an excess of antibody on the cell surface. These results showed that A7-NCS was internalized into target cells and that its cytotoxicity was mediated through specific binding and internalization.View full abstractDownload PDF (1181K) -
KIMIKAZU KOJIMA, AKIHITO ISHIZAKA, EIKI OSHIKA, YUICHI TAGUCHI, KAZUHI ...1990 Volume 161 Issue 3 Pages 209-215
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSKOJIMA, K., ISHIZAKA, A., OSHIKA, E., TAGUCHI, Y., TOMIZAWA, K., NAKANISHI, M., SAKIYAMA, Y. and MATSUMOTO, S. Quantitation of IgG Subclass Antibodies to Pneumococcal Capsular Polysaccharides by ELISA, Using Pneumovax®-Specific Antibodies as a Reference. Tohoku J. Exp. Med., 1990, 161 (3), 209-215-A quantitative enzyme-linked immunosorbent assay (ELISA) method has been developed to assay the levels of IgG subclasses to pneumococcal capsular polysaccharides (PCP) by using a reference standard. This standard solution containing specific antibodies to a polyvalent pneumococcal vaccine (Pneumovax®®) was purified from the serum of an immunized healthy adult by affinity chromatography. In order to determine the predominant response to Pneumovax in the four IgG subclasses, specific IgG subclasses in preimmune and postimmune sera from six healthy adults were assessed quantitatively by the ELISA. With regard to peak concentrations after immunization, there was a marked increase in the IgG2 subclass, compared with those of IgG1 and IgG3. Such a quantitative assay of Pneumovax-specific IgG subclass antibodies is useful for the direct evaluation of immune responses to immunization with a polyvalent pneumococcal vaccine, and at the same time, for estimating the IgG2 response to PCP antigens in individuals.View full abstractDownload PDF (731K) -
YUKITAKA FUKUSHIMA, YASUSHI NAKAMOTO, AKIRA B. MIURA, SHIGERU MIYAGATA ...1990 Volume 161 Issue 3 Pages 217-225
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSFUKUSHIMA, Y., NAKAMOTO, Y., MIURA A.B., MIYAGATA, S. and TSUCHIDA, S. The Inhibitory Factors of Hematopoiesis in Chronic Hemodialysis Patients Treated with Recombinant Human Erythropoietin. Tohoku J. Exp. Med., 1990, 161 (3), 217-225-In order to clarify possible factors responsible for varying responses in uremic patients treated with recombinant human erythropoietin (rHuEPO), we determined the inhibitory effects of ten uremic sera on the erythroid progenitors (CFU-E) and erythroid bursts (BFU-E). We also measured plasma EPO titers, Fe, UIBC, ferritin, PTH-C, β2-microglobulin, and aluminum in all ten patients. The inhibitor of CFU-E but not BFU-E, was present in the serum of the single anemic patient whose recovery took longer after the administration of rHuEPO. He did not have such conditions as iron deficiency, excess of aluminum, or chronic inflammation. The remaining patients, who had no CFU-E or BFU-E inhibitors, were good responders to rHuEPO. In none of ten patients were there inhibitors of granulocyte-macrophage progenitors (CFU-GM) or any differences in the other parameters. Although the inhibitory factor of CFU-E can be overcome with a larger dose, its prior determination may be useful to set out minimal effective dose of EPO treatment.View full abstractDownload PDF (908K) -
NARIHIDE GOSEKI, MITSUO ENDO1990 Volume 161 Issue 3 Pages 227-239
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSGOSEKI, N. and ENDO, M. Thiol Depletion and Chemosensitization on Nimustine Hydrochloride by Methionine-Depleting Total Parenteral Nutrition. Tohoku J. Exp. Med., 1990, 161 (3), 227-239 -As an adjunct to cancer chemotherapy, we had succeeded in creating the methionine depletion in vivo, using the technique of total parenteral nutrition (TPN) by infusing an amino acid solution devoid of methionine and cysteine, as sole nitrogen source (Met-deplet. TPN). In Experiment 1, we demonstrated that the marked depletion of thiol was induced both in the liver and tumor tissues by Met-deplet. TPN in Sato lung carcinoma (SLC)-bearing rats. Then in Experiment 2, we also confirmed the presence of the enhancing effect on nimustine hydrochloride (ACNU) in Met-deplet. TPN to SLC. The tumor proliferation was inhibited significantly by Met-deplet. TPN even in conjunction with a small dose of ACNU, which showed no anti-tumor effect on the rats treated with methionine-containing amino acid solution, without apparent increase of the side effects in comparison with those in the control groups. On the other hand, to determine the carcinostatic effect on tumor-bearing animals, not only the size of the tumor but also its components, especially the percentage of necrotic tumor tissue (necrotic index), were considered to be important.View full abstractDownload PDF (1266K) -
TAKU MATSUBARA1990 Volume 161 Issue 3 Pages 241-250
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSMATSUBARA, T. Beneficial Effects of Low-Flow Perfusion Resumed Early after Zero-Flow Ischemia on Myocardial Energy Metabolism and Mechanical Function: 31P-NMR Study in the Isolated Perfused Rat Heart. Tohoku. J. Exp. Med., 1990, 161(3), 241-250-Effects of low-flow perfusion after zero-flow ischemia on myocardial mechanical function and energy metabolism were studied with 31P nuclear magnetic resonance spectroscopy, using isolated perfused rat hearts. After control perfusion, hearts were randomly divided into five experimental groups: Groups I and II were subjected to zero-flow ischemia of 40 and 60min, respectively. In groups III-V the perfusion was resumed at a rate of 0.1ml/min after 40 (group III), 30 (group IV) and 20 (group V)-min of zero-flow ischemia in order to compare the effects of low-flow perfusion with those of persistent zero-flow. After these interventions all the hearts were perfused for 40min at a normal flow rate. Compared with the hearts exposed to total ischemia of 60min, the preservation of high energy phosphate compounds (HEP) was better in groups with early low-flow perfusion; Creatine phosphate (CrP) levels, which had decreased rapidly after induction of zero-flow ischemia, increased gradually after initiation of the low-flow perfusion and reached significantly higher levels at the end of ischemic period in groups IV and V than in group II (p<0.05). The decrease in adenosine triphosphate (ATP) was likewise significantly suppressed by low-flow perfusion (groups IV and V>group II). Restoration of CrP levels after complete reperfusion was also significantly greater in group V than in group II. The recovery of ATP after complete reperfusion was also much better in group V being comparable to those in group I, although the total duration of ischemia was longer in group V than in group I. These results indicate the beneficial effects of low-flow perfusion on the preservation during ischemia and recovery after reperfusion of myocardial REP.View full abstractDownload PDF (1016K) -
YOSHIAKI TAKAYAMA, SHUICHI ICHIKAWA, TETSUO SAKAMAKI, KAZUHIKO MURATA1990 Volume 161 Issue 3 Pages 251-252
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSTAKAYAMA, Y., ICHIKAWA, S., SAKAMAKI, T. and MURATA, K. Increase in Hematocrit by Nffedipine in Hypertensive Patients. Tohoku J. Exp. Med., 1990, 161 (3), 251-252 -The hematocrit, hemoglobin, serum total protein and serum albumin concentration increased significantly following sublingual administration of nifedipine in patients with essential hypertension (p<0.05). The mean increase in hematocrit at 60min after administration was 1.5%. The results suggest a decrease in plasma volume, perhaps due to diuresis and a shift of fluid from the intravascular to the extravascular space.View full abstractDownload PDF (227K) -
YOSHIYUKI UENO, KOJU KOBAYASHI, HIROSHI SUZUKI, TAKESHI YAMAMOTO, TAKA ...1990 Volume 161 Issue 3 Pages 253-255
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSUENO, Y., KOBAYASHI, K., SUZUZKI, H., YAMAMOTO, T. and TOYOTA, T. Production of Monoclonal Antibodies against Recombinant HbcAg. Tohoku J. Exp. Med., 1990, 161 (3), 253-255-Three monoclonal antibodies against recombinant HBcAg were obtained from hybridomas fused between mouse myeloma line NS1 and splenocytes of immunized Balb/C mice. They specifically bound to recombinant HBcAg. Subtypes of these monoclonal antibodies were IgM and IgA.View full abstractDownload PDF (189K) -
WEISHI CHEN, HIROTO NAKADAIRA, MASASHI TSUNODA, HIROSHI MANO, SHUKO TA ...1990 Volume 161 Issue 3 Pages 257-259
Published: 1990
Released on J-STAGE: August 31, 2006
JOURNAL FREE ACCESSCHEN, W., NAKADAIRA, H., TSUNODA, M., MANO, H., TAKAGI, S., YAMAMOTO, M., KINEBUCHI, H., KATO, K. and AKAI, S. Selenium Contents in Human Gallbladder Bile. Tohoku J. Exp. Med., 1990, 161 (3), 257-259 -The selenium contents in human gallbladder bile were analyzed. Thirty-seven subjects were studied; 22 patients with cholelithiasis in Niigata Prefecture and 15 patients (13 with cholelithiasis and 2 with gallbladder polypus) in Kochi Prefecture. Five ml of bile was withdrawn with a syringe from the gallbladder during the operation and stored at -20°C until analysis. For the analysis by gas chromatograph with an electron-capture detector, 0.2ml of sample was used. The mean selenium contents in bile were 269±39.0 (mean±S.D.) ng/ml for the subjects in Niigata and 285±84.4ng/ml in Kochi; without significant difference. Of 37 samples analyzed, the mean content was 276±61.0ng/ml.View full abstractDownload PDF (327K)
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