巻号一覧
193 巻 (2001)
- 4 号 p. 259-
- 3 号 p. 163-
- 2 号 p. 79-
- 1 号 p. 1-
193 巻, 1 号
January
選択された号の論文の8件中1～8を表示しています
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Regular Contributions
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Yo Tsuchiya, Hisayoshi Hayashi, Yuichi Suzuki2001 年 193 巻 1 号 p. 1-11
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーThe membrane transport mechanism for regulating the intracellular pH value (pHi) was investigated in mouse distal colon crypt cells. pHi was measured by microfluorometry in an isolated crypt fragment loaded with the pH-sensitive fluoroprobe, 2', 7'-bis-(2-carboxyethyl)-5-(6)carboxyfluorescein. The pHi recovery process after acid loading induced by a 40 mM NH4Cl prepulse was almost totally dependent on Na+ in both the presence and absence of CO2/HCO3− in the perfusion solution. In the CO2/HCO3−-free, HEPES-buffered solution, amiloride partially inhibited the pHi recovery rate from acid loading with an ED50 value of 15 μM and maximum inhibition of 83%. In a CO2/HCO3− solution, amiloride inhibited the pHi recovery rate with an ED50 value of 18 μM, which was similar to that in the HEPES-buffered solution, while the rate of pHi recovery remaining in the presence of the maximum effective concentration of amiloride was significantly larger than that in the HEPES-buffered solution. The Na+-dependent pHi recovery from the acid loading was significantly less (by 18%) in the presence of forskolin. These results suggest that the pHi recovery from acid loading was mediated by 1) amiloride-sensitive Na+/H+ exchanger, 2) the amiloride-insensitive Na+/H+ exchanger, and 3) the Na+- and HCO3−-dependent acid extruder. The pHi recovery could be inhibited by cAMP.抄録全体を表示PDF形式でダウンロード (855K) -
Tsukasa Igarashi, Ryo Konno, Satoshi Okamoto, Takuya Moriya, Shinji Sa ...2001 年 193 巻 1 号 p. 13-25
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーOur objective is to investigate the involvement of granule-mediated apoptosis in the cyclic changes of the endometrium. We demonstrated the localization of CD56, perforin, granzyme B and caspase-3 in the endometrium by immunohistochemistry. We also confirmed the localization of perforin by immuno-electron microscopy, and demonstrated apoptosis in endometrial glandular cells by TdT-mediated dUTP-biotin nick end labeling (TUNEL) and electron microscopy. Uterine CD56-positive natural killer (NK) cells expressed perforin and granzyme B in its cytoplasm. Uterine NK cells increased significantly in the endometrial stroma during the secretory phase, and peaked during the late secretory phase. These cells started decreasing in number during the menstrual period. In endometrial glandular cells, caspase-3 and TUNEL-positive cells increased significantly from the late secretory phase, with apoptosis reaching a peak during the menstrual period. Using electron microscopy, we observed uterine NK cells with chromatin rich, segmented nuclei and intracytoplasmic granules in the stroma obtained from late secretory phase endometria. These cells extended projections to the lining of endometrial glandular cells and attached to form a cell-to-cell contact. In addition, nuclear chromatin was observed to have already cohered and small cytoplasmic organelles were beginning to disappear, suggesting that these endometrial glandular cells were undergoing apoptosis. Utilizing immuno-electron microscopy, intracytoplasmic granules in uterine NK cells were stained with anti-perforin antibody. The findings of this study suggest that granule-mediated apoptosis in endometrial glandular cells induced by NK cells expressing perforin and granzyme B may be associated with the onset of menstruation.抄録全体を表示PDF形式でダウンロード (2571K) -
Daizoh Saitoh, Khan Z. Shirani, William G. Cioffi, Takako Kizaki, Hide ...2001 年 193 巻 1 号 p. 27-36
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーTo examine whether thermal injury alters the superoxide dismutase (SOD) concentrations in various types of tissue or plasma, we studied the plasma and tissue Mn- and Cu/Zn-SOD levels in a rodent burn model. The animals were resuscitated with saline (50 mg/kg, ip) immediately following thermal injury and thereafter were sacrificed at either 6 or 24 hours post-burn. The Mn- and Cu/Zn-SOD levels were measured using an enzyme-linked immunosorbent assay (ELISA). The plasma Mn- and Cu/Zn-SOD concentrations significantly increased 6 hours after the injury and positively correlated with the burn size. The kidney Mn-SOD concentrations were significantly higher 24 hours after the injury in the animals with 30% burns than in those with either sham or 50% burn injuries. The lung Cu/Zn-SOD concentrations were also significantly higher 6 hours after the injury in animals with 30% burns than in the other two types above. These findings suggest that the changes in the SOD concentrations after burn injury vary according to the type of SOD and also the type of tissue. As a result, the SOD concentrations may play some role in the early response to thermal trauma.抄録全体を表示PDF形式でダウンロード (655K) -
Tadahiko Ito, Kenji Harada, Goro Takada2001 年 193 巻 1 号 p. 37-44
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーWe studied morphological changes in the left and right ventricles of fetal rats in late-gestation using rapid whole-body freezing technique. Pregnant Wistar rats (term, 21.5 day) were immediately frozen in liquid nitrogen on 17-, 18-, 19-, 20-, and 21-day of gestation. The frozen fetal hearts were serially sectioned with a sliding microtome and photographed. The ventricular volume, mass, wall thickness, and area of valvular orifice were measured on the photographs. During the study period, the left and right ventricular volumes increased very rapidly (9.9-fold and 7.6-fold, respectively) compared with the increase in the body weight (4.0-fold); the volumes divided by body weight increased linearly. Left and right ventricular masses also rapidly increased (5.9-fold and 5.0-fold, respectively). Mass/volume ratios for the two ventricles rapidly decreased. The wall thicknesses divided by body weights rapidly decreased with the progression of the gestational age. The left and right ventricles at 17 day of gestation have relative hypertrophy and relatively large valvular orifices as compared with those in terminal gestation. The improvement of the relative hypertrophy of the ventricles may indicate the morphological and functional maturation of the fetal heart.抄録全体を表示PDF形式でダウンロード (463K) -
Takuma Narita, Jun Koshimura, Hiroyuki Meguro, Hiroji Kitazato, Hiroki ...2001 年 193 巻 1 号 p. 45-55
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーTo establish the method by which the optimal dietary protein content for type 2 diabetic patients with nephropathy could be determined, dietary protein content was reduced in gradated steps and renal function was evaluated at the completion of each diet. Eight type 2 diabetic patients with microalbuminuria were examined in this study. Renal function, urinary albumin excretion rate (AER) and urinary excretion rates of prostaglandins were evaluated at the completion of each of three consecutive one-week dietary periods where the protein content was 1.2, 0.8 and 0.6 g·kg Body Weight (BW)−1·day−1 on the first, second and third week, respectively. Filtration fraction (FF), AER and urinary excretion rates of prostaglandin E2 and 6-keto-prostaglandin F1α significantly decreased in response to reduced dietary protein content from 1.2 to 0.8 g·kg BW−1·day−1. No additional decreases in FF, AER and urinary excretion rates of these two prostaglandins were obtained after the 0.6 g·kg BW−1·day−1 low protein diet period. The method evaluating renal hemodynamics at the completion of several consecutive one-week dietary periods was confirmed to be useful to determine the optimal protein contents in type 2 diabetic patients with nephropathy. The result showed that the optimal protein content in type 2 diabetic patients with microalbuminuria was 0.8 g·kg BW−1·day−1 and protein restriction of less than 0.8 g·kg BW−1·day−1 was not necessary for patients with this stage of diabetic nephropathy. A part of reasons in which FF decreased after reduced protein content in diet may be due to decreased prostaglandins production in the kidneys.抄録全体を表示PDF形式でダウンロード (694K) -
Atsushi Yamadori, Kyoko Suzuki, Masumi Shimada, Takashi Tsukiura, Taka ...2001 年 193 巻 1 号 p. 57-65
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーTwo cases of isolated retrograde amnesia were reported. Both showed the same clinical pattern in development and resolution of amnesia despite of different etiologies. Sudden insult to the brain (trauma in Case 1 and viral encephalitis in Case 2) caused concurrent antero- and retrograde amnesia. Fortunately both recovered from the anterograde amnesia completely. However, both were left with a period of postictal amnesia of a few months and retrograde amnesia of up to 14 months' duration. The analysis of their pattern of temporal evolution and dissolution of amnesia support the hypothesis that recently acquired episodic information requires a certain amount of constant activation for a certain period of time in order to be organized into a durable memory. The nature of this activation as well as its origin remains to be solved.抄録全体を表示PDF形式でダウンロード (654K)
Case Reports
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Mihoko Watanabe, Masahiro Kohzuki, Kenichi Meguro, Yoko Goto, Tokutaro ...2001 年 193 巻 1 号 p. 67-72
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーThis paper reports a case of a 71-year-old pulmonary emphysema patient with neuropsychological impairments that were markedly improved 6 months after he underwent lung volume reduction surgery (LVRS). He also underwent pulmonary rehabilitation before and after surgery. He was suspected of having memory impairment and attention disorder when he was referred for rehabilitation. The neuropsychologic test showed a general cognitive impairment, attention disorder, and verbal memory impairment. Magnetic resonance imaging showed moderate atrophy of the left hippocampal area, cortex, and lacunae infarction in the periventricular area. Interestingly, scores of the neuropsychologic test, as well as severity of dyspnea and lung function, remarkably improved 6 months after LVRS. These results suggested that the neuropsychological impairments in pulmonary emphysema patients can be improved after lung reduction surgery.抄録全体を表示PDF形式でダウンロード (578K) -
Thrombotic Stroke in a Child with Diarrhea-Associated Hemolytic-Uremic Syndrome with a Good RecoveryTohru Nakahata, Hiroshi Tanaka, Takashi Tateyama, Tomomi Ueda, Koichi ...2001 年 193 巻 1 号 p. 73-77
発行日: 2001年
公開日: 2005/02/15
ジャーナル フリーA boy aged 3.5 years with post-diarrheal hemolytic-uremic syndrome (HUS) was referred to our hospital because of convulsion and stupor. He had been admitted to a regional hospital with a 3-day history of bloody diarrhea, colic abdominal pain and fever. Two days later, he had complained of generalized seizures and oliguria. On admission, he developed anuria, and serum blood nitrogen and creatinine increased to 56 mg/100 ml and 2.8 mg/100 ml, respectively. Platelets decreased to 42 000/μl. Under the diagnosis of HUS, a continuous hemodiafiltration treatment had to be instituted. Computed tomography of his head at hospital day 5 revealed abnormal low density area of infarction with edema in both the basal ganglia involving with the posterior limb of internal capsule. Serum titer of IgM antibody to Escherichia coli O157 showed positive value. Although his anuria and stupor persisted over 10 days, he recovered without serious complications. These clinical observations may indicate that patients with similar lesions do not necessarily have serious morbidity.抄録全体を表示PDF形式でダウンロード (539K)
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