I investigated the oxidation of the vitamin C like substance in human milk and that of the ascorbic acid by means of the Arakawa Reagent III and knew that peroxide and guaiacol (or guaiacol-like substance) are necessary for this oxidation. Further, I knew that an addition of the reagent can oxidize a large amount of ascorbic acid, and was able to differentiate between human milk with AR 5' (-)* and Arakawa-positive milk made Arakawa-negative by ascorbic acid.
I examined the blood platelet count of 266 apparently healthy lactating mothers with different Arakawa's reaction. The platelet count of 27 Arakawa-positive mothers was within normal limits in 84% of the cases, and only 15% showed a slight increase of the count, the average count being 340 thousands in all the Arakawa-positive cases. The count of Groups II and III (the groups of the better intermediate Arakawa's reaction) showed a normal count in 43% of the cases, a slight increase in 28%, a moderate increase in 24% and in intense or remarkable increase in 5%, the average count being 431 thousands. The count of Groups III and IV (the groups of the worse intermediate Arakawa's reaction) showed a normal count in 25%, a slight increase in 34%, a moderate increase in 24% and a remarkable increase in 13%, the average count being 487 thousands. The count of Groups VI and VII (the groups of completely or almost completely negative Arakawa's reaction) showed a normal count in only 16%, a slight increase in 36%, a moderate increase in 32% and a remarkable increase in 16%., the average count being 504 thousands (Cf. Table 11). Thus, the platelet count ran parallel to the intensity of Arakawa's reaction.
As stated above, all the 4 rabbits used showed methyl glyoxal like substance in the milk, which showed a certain decrease (up to 2.0 mgrms. %) on a subcutaneous use of vitamin B, until the qualitative tests became negative. When monoiodacetic acid was subcutaneously injected, the methyl glyoxal like substance increased gradually, which was proved both quantitatively and qualitatively. Arakawa's reac-tion became weaker and weaker, as the substance increased. Body weight showed a decrease. These changes due to monoiodacetic acid were recovered by vitamin B1 alone when they were of a light degree (as in Rabbit 2), but, when they were of a serious degree (as in Rabbits 3 and 4), only a combined use of yakriton and vitamin B1 was able to improve the condition. It is to be remarked here, especially because Geiger and Rosenberg10) reported that an administration of vitamin B caused a temporary increase of methyl glyoxal in urine of B-avitaminotic dogs, that temporary increase of methyl glyoxal like substance soon after the use of vitamin B1 was seen in the rabbit's milk (Cf. Rabbits 2 and 3) in my own experiment.
An Kaninchen wiekt die Galle bei der Einführung in den Darmkanal auf den Dickdarm und etwas schwächer auch auf den Dünndarm tonussteigeind und peristaltikbeschleunigend. Diese fördernde Wirkung kommt dadurch zustande, dass die Galle die sensiblen Nervenenden der Schleimhaut angreift and mittelbar den Auerbach schen Nervenplexus erregt. Die Galle hat die Wirkung, direkt den Auerbach schen Nervenplexus zu lähmen, aber dringt in die Darmwand der gesunden Schleim haut aus schwer hinein, so dass these Wirkung bei der Einführung der Galle in den Darmkanal kaum in Betracht kommt. Wenn sie aber in einer genügenden Menge schnell in die Wand hineindringt, wie bei der Applikation auf die seröse Haut, so muss anstatt der fördernden eine hemmende Zustandsänderung eintreten.
The rabbits, well surviving 64 to 160 days the last suprarenal-ectomy were definitely more sensitive to histamine applied hypoder-mically, even though a little, than the normal controls. But the rabbits, well surviving 64 to 118 days the medulli-suprarenalectomy, had quite the same degree of resistance against subcutaneous application of histamine. The subcutaneous maximum non-lethal dose of hista-mine and the minimum lethal dose are respectively 10 mgrms. and 35 ingrms. for the normal rabbit and the doubly medulli-suprarenalec-tomized, and respectively less than 10 mgrms. and just 30 mgrins, for the rabbits, doubly suprarenalectoiized. The loss of medullary tissue from suprarenal glands does not alter, if at all, the susceptibility of the animal, against histamine intoxica-tion, contrary to the view of an American author. The increase of susceptibility to histamine in the doubly suprarenalectomized rabbit is therefore due to insufficiency of the suprarenal cortical tissue.
The common carotid arteries were clamped in the dogs, whose epinephrine secretion was measured by means of the method of Satake and others. No anaesthesia was used; the dog was not fastened. The observation extended for 1-2.5 hours after clamping. The clamping caused blood pressure elevation, but did not bring about any alteration in the epinephrine output rate from the supraren-als nor any change in the blood sugar concentration.