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Volume 86 (1965)
- Issue 4 Pages 301-
- Issue 3 Pages 201-
- Issue 2 Pages 93-
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Volume 86, Issue 1
Displaying 1-10 of 10 articles from this issue
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Yutaka Matsuya1965 Volume 86 Issue 1 Pages 1-8
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA serum-free culture medium was devised which supports the growth of L line cell subcultured in a serum containing usual medium and enables it to propagate from its low density of inoculum. This serum-free medium consists of Eagle's minimum essential medium supplemented with bovine albumin, insulin, serine, pyruvate, protamine (or FeCl3) and lactalbumin hydrolysate in replacement of serum. This medium supported the cellular growth from such low density of the inoculum as that employed in “single cell culture technique” and colony forma-tion was observed with the above technique.View full abstractDownload PDF (808K) -
Kenichi Iwatsuki, Tsuneo Yusa, Yoshifumi Kataoka1965 Volume 86 Issue 1 Pages 9-18
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSSome of the clinically used muscle relaxants have been reported to exhibit certain effects other than muscular relaxation. Among them the effects upon the cardiovascular system are of clinical importance. This paper dealt with the results of investigating the effects of various muscle relaxants upon ventricular contractile force directly measured by the method of a heart-lever system in dogs. d-Tubocurarine chloride caused a marked decrease in ventricular con-tractile force, associated with a concomitant fall in aortic and pulmonary arterial blood pressure. These changes seemed to be attributed mainly to a direct action of histamine released by d-tubocurarine. Succinylcholine chloride resulted in a transitory increase in ventricular contractile force, associated with a slight rise in aortic blood pressure. These changes seemed, to be due to a sympa-thomimetie action of succ.inylcholine. Decamethonium bromide, hexamethylene-1, 6-bis-carbaminoylcholine iodide, gallamine triethiodide as well as diallyl-nor-toxiferine showed little effects upon ventricular contractile force.View full abstractDownload PDF (4452K) -
Tatsuo Koyanagi, Keiko Oikawa1965 Volume 86 Issue 1 Pages 19-22
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn rats fed a riboflavin-deficient diet containing soybean oil, fatty acid con-tent in serum with 4 double bonds was lower, whereas that with 2 double bonds was higher than fatty acid in serum of control animals. The low percentage of fatty acid with 4 double bonds in serum of deficient animals showed perhaps the failure of synthesis of arachidonic acid from linoleic acid in these animals.View full abstractDownload PDF (219K) -
Isamu Kaito, Kazuo Wakui1965 Volume 86 Issue 1 Pages 23-32
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe micromethod technique for the determination of enzyme activities in liver is out lined.
It is demonstrated that the micromethod technique is reliable.
Glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline and acid phosphatases, glucose-6-phosphataso, catalase, aldolase and arginase activities in liver and serum were measured in various liver and malignant diseases.
Routine hepatic function tests were done simultaneously and the results were compared with the results of hepatic enzyme studies. This study showed significant correlation existing only between alkaline phosphatase activity in liver and A/G ratio. Simultaneously done histological studies demonstrated significant correlation existing only between the alkaline phosphatase activity in liver and the grade of fibrosis in the liver. The duration of the hepatic disease correlated significantly with glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities in the liver.
Although the changes in enzyme activities of the serum and the liver in majority of the cases showed concomitant and symmetrical pattern of the changes, enougn number of cases deviated from this general rule, suggesting the necessity of further study in regard to the mechanism of the increase of these enzyme activities in the serum, mode of activation of the liver enzymes, its turnover, in short, the dynamic mechanism of the liver enzymes.View full abstractDownload PDF (646K) -
Kazumori Sakai1965 Volume 86 Issue 1 Pages 33-42
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn dogs anesthetized with sodium pentobarbital, adrenal venous blood was collected and analyzed for adrenaline and noradrenaline by the fluorimetric method. Four groups of experiments were performed: (1) Bleeding in intact dogs. (2) Infusion of dextran or Ringer's solution after bleeding in intact dogs. (3) Bleeding after section of the splanchnic nerves. (4) Bleeding after section of the buffer nerves for blood pressure. A definite increase in adrenal medullary secretion was observed in intact dogs when over one-fourth of the total blood volume was bled. Infusion of dextran or Ringer's solution after bleeding was found to inhibit the adrenal medullary response to hemorrhage. After section of the splanch is nerves the secretion of adrenaline and noradrenaline of the adrenal gland on that side was not increased by bleeding. After section of the buffer nerves for blood pressure the adrenal medullary secretion was increased after bleeding, though there was a delay in reaching high level of adrenal medullary secretion.View full abstractDownload PDF (574K) -
Marked Elevation of Fibrinolytic Activity of Blood Obtained from Vasa Efferentia of the UterusMasahiro Maki, Masataka Nagayama, Kyoko Sasaki, Takeshi Yoneya1965 Volume 86 Issue 1 Pages 43-50
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTissue activator of the plasmin system is easily extracted into such high molar solutions as 1-2 M KSCN, KCl, KI or 30% sucrose, but not into physiological saline solution. A question, therefore, arises as to by what mechanism such activator can enter into the circulation. In order to clarify the activation mechanism of the plasmin system and to determine whether or not the tissue activator can enter into the circulation, fibrinolytic activity of blood obtained from the vasa efferentia of the uterus was determined and compared with that from the cubital vein.
A marked elevation of fibrinolytic activity was observed in blood from the vasa efferentia of the uterus. It was also found that the endometrial tissue activator extracted into KSCN solution was completely transferred to the physiological saline-soluble form after the Sephadex column chromatography.
Thosc findings may suggest one possible mechanism of entrance of the tissue activator into the circulation.View full abstractDownload PDF (401K) -
Sigetosi Kamiyama, Kozo Ishiguro, Yoshiaki Kawakami, Satoru Kobayashi, ...1965 Volume 86 Issue 1 Pages 51-64
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA long-period experimental study on the effect of the diets of different calcium content was conducted using 136 albino rats of Wistar strain for 24 morAbs. Differentiation of mortality and mean body weight among those groups was marked at the first twelve months compared to the second. Balance study between in-take and output, and also quantitative analysis of minerals in tissue and organ speci-mens revealed that the adaptation for the low calcium diet was accomplished by the later stage of life, and the analytical values between groups stayed within individual variation. However histopathological investigation revealed that the specific changes attributable to the low calcium diet appeared as local-ized fibrotic foci scattered in ventricular wall and scattered softening foci of status spongiosus accompanied with glia cell swellings in diencephalon after long-period feeding with the calcium deficient diet.View full abstractDownload PDF (2909K) -
Seigi Tsuchida, Yukio Kimura, Takashi Someno, Hiroatsu Sugawara1965 Volume 86 Issue 1 Pages 65-76
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSUreteral activity during the intravesical infusion was examined by urometry and electro-ureterograpby in 12 normal persons and a patient with vesico-ureteral reflux. The urometrograms and electro-ureterograms in normal persons indicated that when the bladder was distended with physiologic saline solution, the ureteral peristalsis responded so as to overcome the increased intravosical pressure. In the case with vesico-ureteral reflux, the ureteral peristalsis was found to be preserved. The values measured on the urometrogram and electro-ureterogram were all within the range of normal ones, and the urine was transported smoothly from the renal pelvis to the bladder without the reflux, when the intravesical pressure was below 10cm H2O. When the intravesical pressure was raised up to 10cm H2O, the reflux appeared. Even in this ureter persitalsis responded so as to flow out the refluxed urine. These findings suggest that ureteral perisitalis, if it remains, works to repress the veiico-ureteral reflux as far as it can resist the increased intravesical pressure and that it works against the reflux, when it actually occurs.View full abstractDownload PDF (2290K) -
Keishi Abe, Naoya Watanabe, Naofumi Kumagai, Isao Miwa, Koichi Mouri, ...1965 Volume 86 Issue 1 Pages 77-83
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAn assay method for the activity of kininase in human venous blood was described. It consists of the incubation of synthetic bradykinin with venous blood at 37°C for 10 minutes and the biological estimation of bradykinin after the in-cubation.
This method is simple and accurate, and can be easily carried out.
The kininase activities were examined on 32 human subjects by this method. Tho rates of inactivation of bradykinin wore 44_??_72 per cent in 11 healthy persons. In most of the patients under various clinical conditions, the values were similar in range to the normal. The patients with liver disease and some of the leukemic patients showed elevated kininase activities.View full abstractDownload PDF (329K) -
Yutaka Matsuya1965 Volume 86 Issue 1 Pages 84-92
Published: June 25, 1965
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA serum-free medium containing albumin and lactalbumin hydrolysate as the two unidentified components was described in the previous paper. The albumin could not replaced with linoleic acid. One of the action of albumin in the serum-free medium may have an ability to inhibit tryptic digestion of surface of the cells at the time of cell dispersion treatment.
Lactalbumin hydrolysate was regularly less active than Bacto-peptone. The L line cells have been successfully adapted to the serum-free medium from the low inoculum. The problem of adaptation of cells cultured in serum-free media was discussed.View full abstractDownload PDF (1085K)
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