Multi-agent simulations are key technologies to know the behavior of complex systems. Each agent in the multi-agent simulations is designed with probabilistic actions to other agents and environment. Hence, the deterministic assessment of complex systems with multi-agent simulations is difficult. Recently, uncertainty quantification attracts much attention to quantitatively assess the uncertainty of simulation results. In this paper, uncertainty quantification of multi-agent based traffic simulation is conducted and its characteristics are discussed. We conducted a series of traffic simulations in Okayama City based on the actually performed traffic census and investigate the relationship between the number of trials of traffic simulations and their reliability.