Japanese Journal of Tropical Medicine and Hygiene
Online ISSN : 2186-1811
Print ISSN : 0304-2146
ISSN-L : 0304-2146
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Volume 17, Issue 1
Displaying 1-5 of 5 articles from this issue
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  • MORPHOLOGICAL OBSERVATION OF LARVAL AND PUPAL RESPIRATORY APPARATUS OF MANSONIA (MANSONIOIDES) UNIFORMIS (THEOBALD) (DIPTERA, CULICIDAE) BY SCANNING ELECTRON MICROSCOPE
    MISAO IWAKI
    1989 Volume 17 Issue 1 Pages 1-7
    Published: March 15, 1989
    Released on J-STAGE: May 20, 2011
    DOIhttps://doi.org/10.2149/tmh1973.17.1
    JOURNAL FREE ACCESS
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    The respiratory organs of Mansonia uniformis larvae and pupae were minutely observed by scanning electron microscope (SEM). Crown-shaped, gill-like, thin filmy and sponge-like tissues were observed at the tip of the siphon of the 1st and 4th instar larva. Similarly, characteristic structure consisting of fine, slender loop (like capillary blood vessels) was observed at the tip of the pupal trumpet. The auther suggested that immatures of Mansonia take in water soluble oxygen with the respiratory “gills” which exist at the tip of the siphon of the larva and at the tip of the trumpet of the pupa.
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  • IL-2 PRODUCTION INHIBITORY ACTIVITY OF THE SERA IN TRYPANOSOMA BRUCEI GAMBIENSE INFECTED MICE
    DAO TAO, MINORU HIGASA, HIKOSHIRO MIYAMOTO, KAZUHIKO YAMADA, TOSHINORI ...
    1989 Volume 17 Issue 1 Pages 9-15
    Published: March 15, 1989
    Released on J-STAGE: May 20, 2011
    DOIhttps://doi.org/10.2149/tmh1973.17.9
    JOURNAL FREE ACCESS
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    African trypanosome infections result in severe immunosuppression. Previous studies have shown that this suppression is due, at least in part, to suppressor cells (T cells and macrophages) or impairment of IL-2 production.
    In the present study, the effects of Trypanosoma brucei gambiense infection on serum levels of IL-2 inhibitor and Concanavalin A (Con A) induced IL-2 production of spleen cells were investigated. The results showed the ability of infected spleen cells to produce IL-2 was markedly reduced and IL-2 production of normal spleen cells was inhibited by the infected sera. But the IL-2 dependent CTLL-2 cell proliferation was not inhibited by those sera. These results suggest that depletion of IL-2 production during T. b. gambiense infection may be due to the increase of an inhibitor of IL-2 production.
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  • ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) IN THE DETECTION OF HEPATITIS-B SURFACE ANTIGEN IN BLOOD DRIED ON FILTER PAPER
    SHINYA MATSUDA, HIROAKI KAHYO
    1989 Volume 17 Issue 1 Pages 17-25
    Published: March 15, 1989
    Released on J-STAGE: May 20, 2011
    DOIhttps://doi.org/10.2149/tmh1973.17.17
    JOURNAL FREE ACCESS
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    In an attempt to find a convenient method for the seroepidemiological study of HBsAg in developing countries, ELISA using blood samples dried on filter paper was evaluated. The results of this method were in complete agreement with both those obtained on original sera by RIA and with those obtained on sera by ELISA. We did not experience any false positive or false negative results. In this study the sample dried on filter paper was eluted by 1 ml of PBS (pH 7.2). Dilution rates were calculated by the calibration line of each serum. Dilution rates were between 0.041 and 0.082. The coefficient of variation of absorbance value of 10 samples of each case was between 5.0% and 14.8%, which indicated an acceptable intratest reproducibility of this method. In addition, storage at 20°C, 20°C with desiccants and 3°C for up to 21 days did not alter the sensitivity and specificity of this method. This method will be useful for the seroepidemiological study of HBV in developing countries.
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  • DIAGNOSIS OF PNEUMOCYSTIS CARINII IN SPUTUM LIQUEFIED WITH THE MUCOLYTIC AGENT DITHIOTHREITOL (SPUTASOL)
    TSUNEZO SHIOTA
    1989 Volume 17 Issue 1 Pages 27-31
    Published: March 15, 1989
    Released on J-STAGE: May 20, 2011
    DOIhttps://doi.org/10.2149/tmh1973.17.27
    JOURNAL FREE ACCESS
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    1. Light microscopic investigations : Sputasol treatment did not affect the staining properties of either the cyst wall or the parentheses-like structures by Gomori's methenamine silver nitrate (GMS) stain. Detection of Pneumocystis carinii was easier in the Sputasol-treated preparations because it was possible to prepare thin smears of the sediment, and because the cysts were concentrated. The amount of time needed for microscopic examination was reduced to the decreased amount of sediment in the Sputasol-treated samples. 2. Transmission electron microscope investigations : In the ultrastructure of P. carinii cysts stained with GMS stain, the heavier deposition of silver particles on the internally thickened parts of the cyst wall corresponded exactly with the distinctive appearance of the parentheses-like structures seen in light microscopic observations.
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  • Special lecture
    1989 Volume 17 Issue 1 Pages 37-103
    Published: March 15, 1989
    Released on J-STAGE: May 20, 2011
    DOIhttps://doi.org/10.2149/tmh1973.17.37
    JOURNAL FREE ACCESS
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