Japanese Journal of Tropical Medicine and Hygiene
Online ISSN : 2186-1811
Print ISSN : 0304-2146
ISSN-L : 0304-2146
26 巻, 4 号
選択された号の論文の3件中1~3を表示しています
  • KATSUYUKI YUI, AKIHIKO YANO
    1998 年 26 巻 4 号 p. 305-318
    発行日: 1998/12/15
    公開日: 2011/05/20
    ジャーナル フリー
    The cDNA for a member of stress induced 70-kDa protein family (hsp70) from Toxoplasma gondii was cloned. The deduced amino acid sequence revealed a 667 amino acid protein70-80% homologous to other parasite and mammalian hsp70s. Southern blot analysis suggested that it is encoded by an intronless gene. A protein of-77-kDa was identified in a lysate of T. gondii tachyzoites by mAbs generated against the recombinant hsp70 protein. Anti-human hsp70 mAb also cross-reacted with a T gondii protein of the identical molecular weight. However, immunoprecipitation and Western blot analysis of these proteins indicated that it was distinct from the cloned hsp70 product, suggesting that T gondii expresses another hsp 70-like protein. Among 22 mice infected with a low virulence Fukaya strain of T. gondii, 6 mice exhibited significant humoral anti-hsp70 Ab responses. This Ab responses peaked at 1-2 weeks of infection, plateaued for 2-3 weeks and gradually declined to nearly undetectable levels at 6 weeks of infection. In contrast, the levels of serum Ab specific for soluble tachyzoite Ags continued to increase during the infection in all mice examined. These features suggest that pathogen-derived hsp70 may play a unique role in the induction and maintenance of the host immune responses.
  • TETSU YAMASHIRO, SITHAT INSISIENGMAY, YASUKO HONMA, NAOMI HIGA, MIKA E ...
    1998 年 26 巻 4 号 p. 319-322
    発行日: 1998/12/15
    公開日: 2011/05/20
    ジャーナル フリー
    An epidemic of Vibrio parahaemolyticus gastroenteritis with several outbreaks occurred in Vientiane, People's Democratic Republic of Lao (Laos), an inland country in August and September in 1997. Serotypes of the 36 isolated V.parahaemolyticus strains from the patients were all O3 : K6. The organisms grew in peptone water supplemented with 10%NaCl. All isolates were positive for Kanagawa phenomenon, and they were positive for tdh, negative for trh and urease. Genomic patterns were not completely identical, as determined by pulsed-field gel electrophoresis. The causative food was suspected to be a papaya salad containing minced and salted fresh water crab. Although a Kanagawa phenomenon positive V.parahaemolyticus strain was isolated from the suspicious food, the serotype was O2 : K28, tdh and trh were negative.
  • SOMBAT TREEPRASERTSUK, DUANGRUDEE CHINDANOND, POLRAT WILAIRATANA, SRIV ...
    1998 年 26 巻 4 号 p. 323-326
    発行日: 1998/12/15
    公開日: 2011/05/20
    ジャーナル フリー
    Concomitant infection with malaria and filariasis is known to occur in animals and the coinfection appears to lessen the severity of malaria. We report here the incidence of co-infection with filariasis among 4, 201 malaria patients admitted to the Bangkok Hospital for Tropical Diseases, Mahidol University, Thailand, between 1995 and 1997. There were eight patients (0.2%) with microfilariae (all Wuchereria bancrofti) in the peripheral blood smear. Four of the 8 patients had falciparum malaria and two patients among this group had cerebral malaria which responded to treatment without any long term sequelae. The rest four patients, three had vivax malaria while the last one had uncomplicated mixed infection of falciparum and vivax malaria. Filariasis was asymptomatic in all patients and cured with diethylcarbamazine. The eight patients resided along the Thai-Myanmar border, which is known to be endemic for both diseases. Our findings indicate the existence of co-infection of malaria and filariasis in Thailand, especially among patients from the Thai-Myanmar border. From this small number of patients, it is difficult to conclude that filariasis affects the severity of malaria. However, the data does emphasize that early diagnosis and early treatment of both infections is possible.
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