The authors investigated the method for determination of xylitol in blood, and obtained satisfactory results by following procedure; Namely, glucose in blood is decomposed with alkali and eliminated by anion exchange resin. Xylitol is oxidised with sodium periodate and formaldehyide formed is colorated by chromotropic acid. Optical density is measured at 570mμ. According to this method the recovery percent of xylitol in blood is about. 94%. Furthermore, we investigated the changes of xylitol and glucose concentration in blood after administration of xylitol to rabbits.
A Simple and accurate method for the determination of insulin antibody was elaborated. In the technique small and stable polyazostyrene-insulin particles could agglutinate with insulin antibody with high sensitivity. The method has an advantage in its reproducibility, because the polystyrene-insulin latex can be preparedwith the constant amount of insulin as antigen and can be stored under 0°C at least six monthes. It was particulary suitable to determine the antibody to a minor molecular peptide-hormone, insulin.