In order to study on amino acid metabolism in diabetes mellitus, the free plasma amino acids were examined with KLA-2 type Hitachi amino acid autoanalyzer in 34 patients with diabetes mellitus and 12 healthy subjects.
The difference of the free plasma amino acid pattern between diabetics and healthy subjects was observed and the changes in the free plasma amino acid reflecting the various clinical types of diabetes mellitus were analyzed.
Influence of treatments such as diet only, oral hypoglycemic agents or insulin on the plasma free amino acids was studied.
Effect of test meals which contain high, medium or low protein on the free plasma amino acids was also observed.
The results obtained were as follow:
1) Asparatic acid, threonine, glycine and alanine were significantly lower in patients than those in healthy normal subjects.
2) Histidine and arginine were significantly lower in the patients above 50 years of age than those in patients below 49 years old.
3) In the plasma amino acid levels of diabetic patients with FBS above 140mg/dl, arginine, histidine and phenylalanine decreased significantly, compared with those in patients with FBS below 139mg/dl. A tendency of inverse correlation was noted between these amino acids and FBS.
4) In diabetic patients with proteinuria, arginine was significantly decreased but serine was increased.
5) In the patients suffering from diabetes mellitus for one year or more, the plasma level of serine was significantly higher than that in those suffering for 12 months or less.
6) The free plasma amino acid of diabetic patients seemed to have no characteristic pattern in relation to the body types, total serum cholesterol, serum A/G retio or retinal abnormalities.
7) When the patients were controlled on both diet and oral hypoglycemic agents, only cysteine decreased significantly. On the other hand on insulin therapy, serine decreased, but cysteine increased significantly.
8) The free plasma amino acid levels in diabetics seemed to change depending on the protein content of the test meals.
The high protein meal caused increase in the level of arginine, proline, isoleucine and tyrosine. The low protein meal caused increase in the level of aspartic acid. The medium protein meal caused increase in valine, isoleucine, leucine, tyrosine and glycine significantly. A decrease of cysteine was seen by either of these three test meals.
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