The long-term effects of pancreas transplantation on diabetic nephropathy have not been determined. The perfection of a technique for vascularized transplantation in the inbred rat pancreas with or without duodenum has made it possible to conduct such studies in our laboratory. Diabetes was induced by administration of Streptozotocin (65mg) to rats. Urine protein determinations were performed weekly on 24 hour samples. Animals were biopsied as early as 2 months after the onset of diabetes and renal tissue was obtained for light microscopy (H & E, PAS, PAS silver methenamine and trichrome stain) and electron microscopy. Significant alterations were observed from 2 months and increased in severity with time. These changes included an increase in mesangial matrix as demonstrated by light and electron microscopy. These changes were associated with a marked increase in protein excretion. Such changes were not observed in animals receiving pancreatic isografts following induction of diabetes.
Plasma pancreatic glucagon, insulin and glucose were measured hourly in four healthy adult subjects during a 24-hour period of daily life. The antiglucagon serum 30 K used for the immunoassay of pancreatic glucagon proved highly specific for pancreatic glucagon. Plasma glucose and insulin levels increased after each meal and were invariable during the night. The increase of plasma insulin after supper was less than that after breakfast irrespective of its higher blood sugar level, suggesting a lower glucose tolerance in the evening. Plasma pancreatic glucagon showed a fairly constant level during day and night in normal subjects just as the total glucagon level reported by others.